CRISPR Plasmids: gRNAs

The table below lists gRNA plasmids designed to target various genes or genomic regions. It is highly recommended that you determine if your cell type contains the target sequence before using any of these gRNA plasmids and review the publication associated with each plasmid for more information on how it was originally used.

Here are a few questions to consider when choosing a gRNA:

• Does a given gRNA sequence exactly match your genomic target? Variation between a given gRNA sequence and your genomic target may reduce the gRNA activity. The variety in sequence decreases the gRNA activity and increases off-target effect.
• Which species or variant of Cas9 (S. pyogenes, S. aureus etc.) was this gRNA sequence designed for? A given gRNA sequence may only be compatible with a single species or PAM binding variant of Cas9. For instance, wild-type SpCas9 must be used with targets that are upstream of a 5' NGG 3' PAM sequence.
• Which CRISPR application is this gRNA sequence compatible with? CRISPR knockout experiments use targeting sequences within exons, whereas CRISPR activation or repression experiments use targets within promoters.

Do you have gRNAs you'd like to add to the Addgene collection? Click here to start the deposit process and have your plasmids added to the list. You can also email for more information and assistance with the deposit process.

gRNA Plasmids

Please note that the following table lists both published and unpublished plasmids containing gRNA sequences designed to be used with any CRISPR nuclease and function. Please see the publication or plasmid information page, or contact the depositing PI for more information on the experimental use of these plasmids.

	Plasmid	Gene/Insert	Vector Type	PI	Publication
	gRNA_AAVS1-T1	gRNA_AAVS1-T1	Mammalian Expression, CRISPR	Church	RNA-Guided Human Genome Engineering via Cas9. Science. 2013 Jan 3.
	gRNA_AAVS1-T2	gRNA_AAVS1-T2	Mammalian Expression, CRISPR	Church	RNA-Guided Human Genome Engineering via Cas9. Science. 2013 Jan 3.
	gRNA_GFP-T1	gRNA_GFP-T1	Mammalian Expression, CRISPR	Church	RNA-Guided Human Genome Engineering via Cas9. Science. 2013 Jan 3.
	gRNA_GFP-T2	gRNA_GFP-T2	Mammalian Expression, CRISPR	Church	RNA-Guided Human Genome Engineering via Cas9. Science. 2013 Jan 3.
	gRNA_DNMT3a-T1	gRNA_DNMT3a-T1 (Homo sapiens)	Mammalian Expression, CRISPR	Church	RNA-Guided Human Genome Engineering via Cas9. Science. 2013 Jan 3.
	gRNA_DNMT3a-T2	gRNA_DNMT3a-T2 (Homo sapiens)	Mammalian Expression, CRISPR	Church	RNA-Guided Human Genome Engineering via Cas9. Science. 2013 Jan 3.
	gRNA_DNMT3b	gRNA_DNMT3b (Homo sapiens)	Mammalian Expression, CRISPR	Church	RNA-Guided Human Genome Engineering via Cas9. Science. 2013 Jan 3.
	Zebrafish-gRNA-0001	gRNA-apoea (Danio rerio)	CRISPR ; zebrafish expression	Joung	Efficient genome editing in zebrafish using a CRISPR-Cas system. Nat Biotechnol. 2013 Jan 29. doi: 10.1038/nbt.2501.
	Zebrafish-gRNA-0002	gRNA-drd3 (Danio rerio)	CRISPR ; zebrafish expression	Joung	Efficient genome editing in zebrafish using a CRISPR-Cas system. Nat Biotechnol. 2013 Jan 29. doi: 10.1038/nbt.2501.
	Zebrafish-gRNA-0003	gRNA-fh site #1 (Danio rerio)	CRISPR ; zebrafish expression	Joung	Efficient genome editing in zebrafish using a CRISPR-Cas system. Nat Biotechnol. 2013 Jan 29. doi: 10.1038/nbt.2501.
	Zebrafish-gRNA-0004	gRNA-fh site #2 (Danio rerio)	CRISPR ; zebrafish expression	Joung	Efficient genome editing in zebrafish using a CRISPR-Cas system. Nat Biotechnol. 2013 Jan 29. doi: 10.1038/nbt.2501.
	Zebrafish-gRNA-0005	gRNA-gsk3b (Danio rerio)	CRISPR ; zebrafish expression	Joung	Efficient genome editing in zebrafish using a CRISPR-Cas system. Nat Biotechnol. 2013 Jan 29. doi: 10.1038/nbt.2501.
	Zebrafish-gRNA-0006	gRNA-rgs4 (Danio rerio)	CRISPR ; zebrafish expression	Joung	Efficient genome editing in zebrafish using a CRISPR-Cas system. Nat Biotechnol. 2013 Jan 29. doi: 10.1038/nbt.2501.
	Zebrafish-gRNA-0007	gRNA-th1 (Danio rerio)	CRISPR ; zebrafish expression	Joung	Efficient genome editing in zebrafish using a CRISPR-Cas system. Nat Biotechnol. 2013 Jan 29. doi: 10.1038/nbt.2501.
	Zebrafish-gRNA-0008	gRNA-tia1l (Danio rerio)	CRISPR ; zebrafish expression	Joung	Efficient genome editing in zebrafish using a CRISPR-Cas system. Nat Biotechnol. 2013 Jan 29. doi: 10.1038/nbt.2501.
	Zebrafish-gRNA-0009	gRNA-tph1a (Danio rerio)	CRISPR ; zebrafish expression	Joung	Efficient genome editing in zebrafish using a CRISPR-Cas system. Nat Biotechnol. 2013 Jan 29. doi: 10.1038/nbt.2501.
	pX261-U6-DR-hEmx1-DR-Cbh-NLS-hSpCas9-NLS-H1-shorttracr-PGK-puro	humanized S. pyogenes Cas9	Mammalian Expression, CRISPR	Zhang	Multiplex Genome Engineering Using CRISPR/Cas Systems. Science. 2013 Jan 3.
	p426-SNR52p-gRNA.CAN1.Y-SUP4t	CAN1.y gRNA (Saccharomyces cerevisiae)	Yeast Expression, CRISPR	Church	Genome engineering in Saccharomyces cerevisiae using CRISPR-Cas systems. Nucleic Acids Res
	pCRISPR::rpsL	CRISPR::rpsL	CRISPR ; E.coli	Marraffini	RNA-guided editing of bacterial genomes using CRISPR-Cas systems. Nat Biotechnol. 2013 Jan 29. doi: 10.1038/nbt.2508.
	PU6::unc-119_sgRNA	unc-119 targeting sgRNA (Synthetic)	CRISPR	Calarco	Heritable genome editing in C. elegans via a CRISPR-Cas9 system. Nat Methods. 2013 Jun 30. doi: 10.1038/nmeth.2532.
	PU6::klp-12_sgRNA	klp-12 targeting sgRNA (Synthetic)	Worm Expression, CRISPR	Calarco	Heritable genome editing in C. elegans via a CRISPR-Cas9 system. Nat Methods. 2013 Jun 30. doi: 10.1038/nmeth.2532.
	pT7EGFPgRNA	egfp target (Synthetic)	CRISPR	Chen	Efficient multiplex biallelic zebrafish genome editing using a CRISPR nuclease system. Proc Natl Acad Sci U S A. 2013 Aug 5.
	pT7tyrgRNA	tyr gRNA (Danio rerio)	CRISPR	Chen	Efficient multiplex biallelic zebrafish genome editing using a CRISPR nuclease system. Proc Natl Acad Sci U S A. 2013 Aug 5.
	pU6-sgGFP-NT1	sgGFP-NT1	Mammalian Expression, Lentiviral, CRISPR	Qi	CRISPR-Mediated Modular RNA-Guided Regulation of Transcription in Eukaryotes. Cell. 2013 Jul 9. pii: S0092-8674(13)00826-X. doi: 10.1016/j.cell.2013.06.044.
	pU6-sgGAL4-1	sgGAL4-1, Puromycin resistance and mCherry	Mammalian Expression, Lentiviral, CRISPR	Qi	CRISPR-Mediated Modular RNA-Guided Regulation of Transcription in Eukaryotes. Cell. 2013 Jul 9. pii: S0092-8674(13)00826-X. doi: 10.1016/j.cell.2013.06.044.
	pU6-sgGAL4-4	sgGAL4-4, Puromycin resistance and mCherry	Mammalian Expression, Lentiviral, CRISPR	Qi	CRISPR-Mediated Modular RNA-Guided Regulation of Transcription in Eukaryotes. Cell. 2013 Jul 9. pii: S0092-8674(13)00826-X. doi: 10.1016/j.cell.2013.06.044.
	pU6-sgCXCR4-2	sgCXCR4 -2 (Homo sapiens), Puromycin resistance and mCherry	Mammalian Expression, Lentiviral, CRISPR	Qi	CRISPR-Mediated Modular RNA-Guided Regulation of Transcription in Eukaryotes. Cell. 2013 Jul 9. pii: S0092-8674(13)00826-X. doi: 10.1016/j.cell.2013.06.044.
	pU6-sgCD71-2	sgCD71 -2 (Homo sapiens), Puromycin resistance and mCherry	Mammalian Expression, Lentiviral, CRISPR	Qi	CRISPR-Mediated Modular RNA-Guided Regulation of Transcription in Eukaryotes. Cell. 2013 Jul 9. pii: S0092-8674(13)00826-X. doi: 10.1016/j.cell.2013.06.044.
	pSNR52-sgTEF1	sgTEF1 promoter (Saccharomyces cerevisiae)	Yeast Expression, CRISPR	Weissman	CRISPR-Mediated Modular RNA-Guided Regulation of Transcription in Eukaryotes. Cell. 2013 Jul 9. pii: S0092-8674(13)00826-X. doi: 10.1016/j.cell.2013.06.044.
	pSNR52-sgTET	sgRNA targeting endogenous TRE elements of pTET07 promoter (Saccharomyces cerevisiae)	Yeast Expression, CRISPR	Weissman	CRISPR-Mediated Modular RNA-Guided Regulation of Transcription in Eukaryotes. Cell. 2013 Jul 9. pii: S0092-8674(13)00826-X. doi: 10.1016/j.cell.2013.06.044.
	pICH86966::AtU6p::sgRNA_PDS	AtU6p::sgRNA_PDS (Synthetic)	CRISPR ; Plant expression	Kamoun	Targeted mutagenesis in the model plant Nicotiana benthamiana using Cas9 RNA-guided endonuclease. Nat Biotechnol. 2013 Aug 8;31(8):691-3. doi: 10.1038/nbt.2655.
	pVC297 VEGF Site#1	gRNA-VEGF site 1 (Homo sapiens)	Mammalian Expression, CRISPR	Joung	High-frequency off-target mutagenesis induced by CRISPR-Cas nucleases in human cells. Nat Biotechnol. 2013 Jun 23. doi: 10.1038/nbt.2623.
	pVC299 VEGF Site#2	gRNA-VEGF site 2 (Homo sapiens)	Mammalian Expression, CRISPR	Joung	High-frequency off-target mutagenesis induced by CRISPR-Cas nucleases in human cells. Nat Biotechnol. 2013 Jun 23. doi: 10.1038/nbt.2623.
	pVC228 VEGF Site#3	gRNA-VEGF site 3 (Homo sapiens)	Mammalian Expression, CRISPR	Joung	High-frequency off-target mutagenesis induced by CRISPR-Cas nucleases in human cells. Nat Biotechnol. 2013 Jun 23. doi: 10.1038/nbt.2623.
	pFYF1548 EMX1	gRNA-EMX1 (Homo sapiens)	Mammalian Expression, CRISPR	Joung	High-frequency off-target mutagenesis induced by CRISPR-Cas nucleases in human cells. Nat Biotechnol. 2013 Jun 23. doi: 10.1038/nbt.2623.
	pDR366 RNF2	gRNA-RNF2 (Homo sapiens)	Mammalian Expression, CRISPR	Joung	High-frequency off-target mutagenesis induced by CRISPR-Cas nucleases in human cells. Nat Biotechnol. 2013 Jun 23. doi: 10.1038/nbt.2623.
	pDR348 FANCF	gRNA-FANCF (Homo sapiens)	Mammalian Expression, CRISPR	Joung	High-frequency off-target mutagenesis induced by CRISPR-Cas nucleases in human cells. Nat Biotechnol. 2013 Jun 23. doi: 10.1038/nbt.2623.
	pFYF1320 EGFP Site#1	gRNA-EGFP site 1 (Synthetic)	Mammalian Expression, CRISPR	Joung	High-frequency off-target mutagenesis induced by CRISPR-Cas nucleases in human cells. Nat Biotechnol. 2013 Jun 23. doi: 10.1038/nbt.2623.
	pFYF1327 EGFP Site#2	gRNA-EGFP site 2 (Synthetic)	Mammalian Expression, CRISPR	Joung	High-frequency off-target mutagenesis induced by CRISPR-Cas nucleases in human cells. Nat Biotechnol. 2013 Jun 23. doi: 10.1038/nbt.2623.
	pFYF1328 EGFP Site#3	gRNA-EGFP site 3 (Synthetic)	Mammalian Expression, CRISPR	Joung	High-frequency off-target mutagenesis induced by CRISPR-Cas nucleases in human cells. Nat Biotechnol. 2013 Jun 23. doi: 10.1038/nbt.2623.
	pDD122 (Peft-3::Cas9 + ttTi5605 sgRNA)	Cas9 (Synthetic), ttTi5605 sgRNA (Other)	Worm Expression, CRISPR	Goldstein	Engineering the Caenorhabditis elegans genome using Cas9-triggered homologous recombination. Nat Methods. 2013 Sep 1. doi: 10.1038/nmeth.2641.
	pSimpleII-U6-tracr-U6-crRNA(tdTomato)-NLS-NmCas9-HA-NLS(s)	NmCas9 (Other), U6pr-tracrRNA (Other), tdTomato crRNA (Synthetic)	Mammalian Expression, CRISPR	Thomson	Efficient genome engineering in human pluripotent stem cells using Cas9 from Neisseria meningitidis. Proc Natl Acad Sci U S A. 2013 Aug 12.
	pSimpleII-U6-tracr-U6-crRNA(OCT4)-NLS-NmCas9-HA-NLS(s)	NmCas9 (Other), U6pr-tracrRNA (Other), OCT4 crRNA (Synthetic)	Mammalian Expression, CRISPR	Thomson	Efficient genome engineering in human pluripotent stem cells using Cas9 from Neisseria meningitidis. Proc Natl Acad Sci U S A. 2013 Aug 12.
	pSTBlue-U6-sgRNA(tdTomato)	sgRNA-tdTomato (Synthetic)	Mammalian Expression, CRISPR	Thomson	Efficient genome engineering in human pluripotent stem cells using Cas9 from Neisseria meningitidis. Proc Natl Acad Sci U S A. 2013 Aug 12.
	pT7goldRNA	golden gRNA (Danio rerio)	CRISPR	Chen	Efficient multiplex biallelic zebrafish genome editing using a CRISPR nuclease system. Proc Natl Acad Sci U S A. 2013 Aug 5.
	pT7mitfagRNA	mitfa gRNA (Danio rerio)	CRISPR	Chen	Efficient multiplex biallelic zebrafish genome editing using a CRISPR nuclease system. Proc Natl Acad Sci U S A. 2013 Aug 5.
	pT7ddx19gRNA	ddx19 gRNA (Danio rerio)	CRISPR	Wente	Efficient multiplex biallelic zebrafish genome editing using a CRISPR nuclease system. Proc Natl Acad Sci U S A. 2013 Aug 5.
	PM-SP!TA	Bacterial SP crRNA to prototspacer A (Synthetic)	CRISPR	Church	Orthogonal Cas9 proteins for RNA-guided gene regulation and editing. Nat Methods. 2013 Sep 29. doi: 10.1038/nmeth.2681.
	PM-SP!TB	Bacterial SP crRNA to prototspacer B (Synthetic)	CRISPR	Church	Orthogonal Cas9 proteins for RNA-guided gene regulation and editing. Nat Methods. 2013 Sep 29. doi: 10.1038/nmeth.2681.
	PM-NM!TA	Bacterial NM crRNA to prototspacer A (Synthetic)	CRISPR	Church	Orthogonal Cas9 proteins for RNA-guided gene regulation and editing. Nat Methods. 2013 Sep 29. doi: 10.1038/nmeth.2681.
	PM-NM!TB	Bacterial NM crRNA to prototspacer B (Synthetic)	CRISPR	Church	Orthogonal Cas9 proteins for RNA-guided gene regulation and editing. Nat Methods. 2013 Sep 29. doi: 10.1038/nmeth.2681.
	PM-ST1!TA	Bacterial ST1 crRNA to prototspacer A (Synthetic)	CRISPR	Church	Orthogonal Cas9 proteins for RNA-guided gene regulation and editing. Nat Methods. 2013 Sep 29. doi: 10.1038/nmeth.2681.
	PM-ST1!TB	Bacterial ST1 crRNA to prototspacer B (Synthetic)	CRISPR	Church	Orthogonal Cas9 proteins for RNA-guided gene regulation and editing. Nat Methods. 2013 Sep 29. doi: 10.1038/nmeth.2681.
	PM-TD!TA	Bacterial TD crRNA to prototspacer A (Synthetic)	CRISPR	Church	Orthogonal Cas9 proteins for RNA-guided gene regulation and editing. Nat Methods. 2013 Sep 29. doi: 10.1038/nmeth.2681.
	PM-TD!TB	Bacterial TD crRNA to prototspacer B (Synthetic)	CRISPR	Church	Orthogonal Cas9 proteins for RNA-guided gene regulation and editing. Nat Methods. 2013 Sep 29. doi: 10.1038/nmeth.2681.
	EE-SP!gIII	Cas9 (Other), anti-gIII tracrRNA (Synthetic)	CRISPR	Church	Orthogonal Cas9 proteins for RNA-guided gene regulation and editing. Nat Methods. 2013 Sep 29. doi: 10.1038/nmeth.2681.
	M-SP-sgRNA	sgRNA targeting GTCCCCTCCACCCCACAGTG, compatible with S. pyogenes Cas9, hU6 promoter (Synthetic)	CRISPR	Church	Orthogonal Cas9 proteins for RNA-guided gene regulation and editing. Nat Methods. 2013 Sep 29. doi: 10.1038/nmeth.2681.
	M-ST1-sgRNA	sgRNA targeting GTCCCCTCCACCCCACAGTG, compatible with S. thermophilus #1 Cas9, hU6 promoter (Synthetic)	CRISPR	Church	Orthogonal Cas9 proteins for RNA-guided gene regulation and editing. Nat Methods. 2013 Sep 29. doi: 10.1038/nmeth.2681.
	M-NM-sgRNA	sgRNA targeting GTCCCCTCCACCCCACAGTG, compatible with N. meningitidis Cas9, hU6 promoter (Synthetic)	CRISPR	Church	Orthogonal Cas9 proteins for RNA-guided gene regulation and editing. Nat Methods. 2013 Sep 29. doi: 10.1038/nmeth.2681.
	pAc-y1sgRNA-Cas9	Cas9 (Synthetic), dU6-sgRNA (Drosophila melanogaster), y1 sgRNA (Drosophila melanogaster)	Insect Expression, CRISPR	Liu	Mutagenesis and homologous recombination in Drosophila cell lines using CRISPR/Cas9. Biol Open. 2013 Dec 10. pii: bio.20137120v1. doi: 10.1242/bio.20137120.
	pAGM4723::AtU6p::sgRNA2-2x35S-5′UTR::Cas9::NOST-AtU6p::sgRNA1	sgRNA_PDS2-Cas9-sgRNA_PDS1 (Synthetic)	Plant Expression, CRISPR	Kamoun	Plant genome editing made easy: targeted mutagenesis in model and crop plants using the CRISPR/Cas system. Plant Methods. 2013 Oct 11;9(1):39.
	pLX-sgRNA	sgAAVS1	Mammalian Expression, Lentiviral, CRISPR	Sabatini	Genetic screens in human cells using the CRISPR-Cas9 system. Science. 2014 Jan 3;343(6166):80-4. doi: 10.1126/science.1246981. Epub 2013 Dec 12.
	pX330-Cetn1/1	Cetn1 sgRNA1 (Synthetic), humanized S. pyogenes Cas9 (Other)	Mammalian Expression, CRISPR	Ikawa	Generation of mutant mice by pronuclear injection of circular plasmid expressing Cas9 and single guided RNA. Sci Rep. 2013 Nov 27;3:3355. doi: 10.1038/srep03355.
	pLKO.1-puro U6 sgRNA Oct4A -12	Oct4A -12 sgRNA (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Wolfe	Cas9 effector-mediated regulation of transcription and differentiation in human pluripotent stem cells. Development. 2014 Jan;141(1):219-23. doi: 10.1242/dev.103341.
	pLKO.1-puro U6 sgRNA Oct4A -158	Oct4A -158 sgRNA (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Wolfe	Cas9 effector-mediated regulation of transcription and differentiation in human pluripotent stem cells. Development. 2014 Jan;141(1):219-23. doi: 10.1242/dev.103341.
	pLKO.1-puro U6 sgRNA SOX17 -91	Sox17 -91 sgRNA (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Wolfe	Cas9 effector-mediated regulation of transcription and differentiation in human pluripotent stem cells. Development. 2014 Jan;141(1):219-23. doi: 10.1242/dev.103341.
	pLKO.1-puro U6 sgRNA SOX17 -126	Sox17 -126 sgRNA (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Wolfe	Cas9 effector-mediated regulation of transcription and differentiation in human pluripotent stem cells. Development. 2014 Jan;141(1):219-23. doi: 10.1242/dev.103341.
	pLKO.1-puro U6 sgRNA SOX17 -177	Sox17 -177 sgRNA (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Wolfe	Cas9 effector-mediated regulation of transcription and differentiation in human pluripotent stem cells. Development. 2014 Jan;141(1):219-23. doi: 10.1242/dev.103341.
	pLKO.1-puro U6 sgRNA SOX17 -296	Sox17-296 sgRNA (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Wolfe	Cas9 effector-mediated regulation of transcription and differentiation in human pluripotent stem cells. Development. 2014 Jan;141(1):219-23. doi: 10.1242/dev.103341.
	pLKO.1-puro U6 sgRNA CAG	negative control sgRNA (Other)	Mammalian Expression, Lentiviral, CRISPR	Wolfe	Cas9 effector-mediated regulation of transcription and differentiation in human pluripotent stem cells. Development. 2014 Jan;141(1):219-23. doi: 10.1242/dev.103341.
	pSLQ1651-sgTelomere(F+E)	Optimized sgRNA (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Qi	Dynamic Imaging of Genomic Loci in Living Human Cells by an Optimized CRISPR/Cas System. Cell. 2013 Dec 19;155(7):1479-91. doi: 10.1016/j.cell.2013.12.001.
	pSLQ1661-sgMUC4-E3(F+E)	optimized sgRNA (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Qi	Dynamic Imaging of Genomic Loci in Living Human Cells by an Optimized CRISPR/Cas System. Cell. 2013 Dec 19;155(7):1479-91. doi: 10.1016/j.cell.2013.12.001.
	pRS316-RGR-GFP	RGR-GFP (Synthetic)	Bacterial Expression, Yeast Expression, CRISPR	Zhao	Self-processing of ribozyme-flanked RNAs into guide RNAs in vitro and in vivo for CRISPR-mediated genome editing. J Integr Plant Biol. 2013 Dec 30. doi: 10.1111/jipb.12152.
	pRS316-RGR-GFP-mHH	RGR-GFP (Synthetic)	Bacterial Expression, Yeast Expression, CRISPR	Zhao	Self-processing of ribozyme-flanked RNAs into guide RNAs in vitro and in vivo for CRISPR-mediated genome editing. J Integr Plant Biol. 2013 Dec 30. doi: 10.1111/jipb.12152.
	pRS316-RGR-GFP-mHDV	RGR-GFP (Synthetic)	Bacterial Expression, Yeast Expression, CRISPR	Zhao	Self-processing of ribozyme-flanked RNAs into guide RNAs in vitro and in vivo for CRISPR-mediated genome editing. J Integr Plant Biol. 2013 Dec 30. doi: 10.1111/jipb.12152.
	pRS316-RGR-GFP-mm	RGR-GFP (Synthetic)	Bacterial Expression, Yeast Expression, CRISPR	Zhao	Self-processing of ribozyme-flanked RNAs into guide RNAs in vitro and in vivo for CRISPR-mediated genome editing. J Integr Plant Biol. 2013 Dec 30. doi: 10.1111/jipb.12152.
	lentiCRISPR - EGFP sgRNA 1	Cas9 (Synthetic), Puromycin resistance (Other), EGFP sgRNA 1 (Synthetic)	Mammalian Expression, Lentiviral, CRISPR	Zhang	Genome-scale CRISPR-Cas9 knockout screening in human cells. Science. 2014 Jan 3;343(6166):84-7. doi: 10.1126/science.1247005. Epub 2013 Dec 12.
	lentiCRISPR - EGFP sgRNA 2	Cas9 (Synthetic), Puromycin resistance (Other), EGFP sgRNA 2 (Synthetic)	Mammalian Expression, Lentiviral, CRISPR	Zhang	Genome-scale CRISPR-Cas9 knockout screening in human cells. Science. 2014 Jan 3;343(6166):84-7. doi: 10.1126/science.1247005. Epub 2013 Dec 12.
	lentiCRISPR - EGFP sgRNA 3	Cas9 (Synthetic), Puromycin resistance (Other), EGFP sgRNA 3 (Synthetic)	Mammalian Expression, Lentiviral, CRISPR	Zhang	Genome-scale CRISPR-Cas9 knockout screening in human cells. Science. 2014 Jan 3;343(6166):84-7. doi: 10.1126/science.1247005. Epub 2013 Dec 12.
	lentiCRISPR - EGFP sgRNA 4	Cas9 (Synthetic), Puromycin resistance (Other), EGFP sgRNA 4 (Synthetic)	Mammalian Expression, Lentiviral, CRISPR	Zhang	Genome-scale CRISPR-Cas9 knockout screening in human cells. Science. 2014 Jan 3;343(6166):84-7. doi: 10.1126/science.1247005. Epub 2013 Dec 12.
	lentiCRISPR - EGFP sgRNA 5	Cas9 (Synthetic), Puromycin resistance (Other), EGFP sgRNA 5 (Synthetic)	Lentiviral, CRISPR	Zhang	Genome-scale CRISPR-Cas9 knockout screening in human cells. Science. 2014 Jan 3;343(6166):84-7. doi: 10.1126/science.1247005. Epub 2013 Dec 12.
	lentiCRISPR - EGFP sgRNA 6	Cas9 (Synthetic), Puromycin resistance (Other), EGFP sgRNA 6 (Synthetic)	Mammalian Expression, Lentiviral, CRISPR	Zhang	Genome-scale CRISPR-Cas9 knockout screening in human cells. Science. 2014 Jan 3;343(6166):84-7. doi: 10.1126/science.1247005. Epub 2013 Dec 12.
	pMZ284	rrk1-sgRNA (Schizosaccharomyces pombe)	Yeast Expression, CRISPR	Zaratiegui	Implementation of the CRISPR-Cas9 system in fission yeast. Nat Commun. 2014 Oct 29;5:5344. doi: 10.1038/ncomms6344.
	pMZ285	rrk1-sgRNA (Schizosaccharomyces pombe)	Yeast Expression, CRISPR	Zaratiegui	Implementation of the CRISPR-Cas9 system in fission yeast. Nat Commun. 2014 Oct 29;5:5344. doi: 10.1038/ncomms6344.
	pMZ286	rrk1-sgRNA (Schizosaccharomyces pombe)	Yeast Expression, CRISPR	Zaratiegui	Implementation of the CRISPR-Cas9 system in fission yeast. Nat Commun. 2014 Oct 29;5:5344. doi: 10.1038/ncomms6344.
	pUC119-gRNA	guide RNA targeting AtPDS3 (Arabidopsis thaliana)	CRISPR ; Plant expression	Sheen	Multiplex and homologous recombination-mediated genome editing in Arabidopsis and Nicotiana benthamiana using guide RNA and Cas9. Nat Biotechnol. 2013 Aug;31(8):688-91. doi: 10.1038/nbt.2654.
	pSAG1::CAS9-U6::sgUPRT	SAG1 5' UTR (Other)	CRISPR ; Toxoplasma expression	Sibley	Efficient Gene Disruption in Diverse Strains of Toxoplasma gondii Using CRISPR/CAS9. MBio. 2014 May 13;5(3). pii: e01114-14. doi: 10.1128/mBio.01114-14.
	CMVp-dsRed2-Triplex-28-gRNA1-28-pA	dsRed2 (Homo sapiens)	Mammalian Expression, Synthetic Biology	Lu	Multiplexed and Programmable Regulation of Gene Networks with an Integrated RNA and CRISPR/Cas Toolkit in Human Cells. Mol Cell. 2014 May 14. pii: S1097-2765(14)00355-4. doi: 10.1016/j.molcel.2014.04.022.
	CMVp-dsRed2-Triplex-HHRibo-gRNA1-HDVRibo-pA	dsRed2 (Homo sapiens)	Mammalian Expression, Synthetic Biology	Lu	Multiplexed and Programmable Regulation of Gene Networks with an Integrated RNA and CRISPR/Cas Toolkit in Human Cells. Mol Cell. 2014 May 14. pii: S1097-2765(14)00355-4. doi: 10.1016/j.molcel.2014.04.022.
	CMVp-dsRed2-Triplex-28-gRNA3-28-gRNA4-28-gRNA5-28-gRNA6-28-pA	dsRed2 (Homo sapiens)	Mammalian Expression, Synthetic Biology	Lu	Multiplexed and Programmable Regulation of Gene Networks with an Integrated RNA and CRISPR/Cas Toolkit in Human Cells. Mol Cell. 2014 May 14. pii: S1097-2765(14)00355-4. doi: 10.1016/j.molcel.2014.04.022.
	pCCM935	unc-22 sgRNA (Caenorhabditis elegans)	Worm Expression	Mello	A Co-CRISPR Strategy for Efficient Genome Editing in Caenorhabditis elegans. Genetics. 2014 May 30. pii: genetics.114.166389.
	pAdSh.U6.gRNAS1	U6.gRNAS1 cassette (Synthetic)	Mammalian Expression, Adenoviral, CRISPR	Goncalves	Adenoviral vector delivery of RNA-guided CRISPR/Cas9 nuclease complexes induces targeted mutagenesis in a diverse array of human cells. Sci Rep. 2014 May 29;4:5105. doi: 10.1038/srep05105.
	pAdSh.U6.gRNAGFP	U6.gRNAGFP cassette (Synthetic)	Mammalian Expression, Adenoviral, CRISPR	Goncalves	Adenoviral vector delivery of RNA-guided CRISPR/Cas9 nuclease complexes induces targeted mutagenesis in a diverse array of human cells. Sci Rep. 2014 May 29;4:5105. doi: 10.1038/srep05105.
	pCCM936	avr-14 sgRNA (Caenorhabditis elegans)	Worm Expression, CRISPR	Mello	A Co-CRISPR Strategy for Efficient Genome Editing in Caenorhabditis elegans. Genetics. 2014 May 30. pii: genetics.114.166389.
	pCCM937	avr-15 sgRNA (Caenorhabditis elegans)	Worm Expression, CRISPR	Mello	A Co-CRISPR Strategy for Efficient Genome Editing in Caenorhabditis elegans. Genetics. 2014 May 30. pii: genetics.114.166389.
	pXPR_011	EGFP sgRNA (Synthetic)	Mammalian Expression, Lentiviral, CRISPR	Root	Rational design of highly active sgRNAs for CRISPR-Cas9-mediated gene inactivation. Nat Biotechnol. 2014 Sep 3. doi: 10.1038/nbt.3026.
	Human BLIMP1 sgRNA	hBLIMP1 sgRNA (Homo sapiens)	Mammalian Expression, CRISPR	Hanna	SOX17 Is a Critical Specifier of Human Primordial Germ Cell Fate Cell 160, 1–16, January 15, 2015
	Human SOX17 sgRNA	hSox17 sgRNA (Homo sapiens)	Mammalian Expression, CRISPR	Hanna	SOX17 Is a Critical Specifier of Human Primordial Germ Cell Fate Cell 160, 1–16, January 15, 2015
	Human T (BRACHYURY) sgRNA	hT sgRNA (Homo sapiens)	Mammalian Expression, CRISPR	Hanna	SOX17 Is a Critical Specifier of Human Primordial Germ Cell Fate Cell 160, 1–16, January 15, 2015
	pSAG1::CAS9-U6::sg290860-6	CRISPR sg290860-6	CRISPR ; ; Toxoplasma gondii	Sibley	Genetic mapping reveals that sinefungin resistance in Toxoplasma gondii is controlled by a putative amino acid transporter locus that can be used as a negative selectable marker. Eukaryot Cell. 2014 Dec 5. pii: EC.00229-14.
	pMZ288	Cas9 (Other), rrk1:sgRNA (Other)	Yeast Expression, CRISPR	Zaratiegui	Implementation of the CRISPR-Cas9 system in fission yeast. Nat Commun. 2014 Oct 29;5:5344. doi: 10.1038/ncomms6344.
	pMZ289	Cas9 (Other), rrk1:sgRNA (Other)	Yeast Expression, CRISPR	Zaratiegui	Implementation of the CRISPR-Cas9 system in fission yeast. Nat Commun. 2014 Oct 29;5:5344. doi: 10.1038/ncomms6344.
	pMZ381	Cas9 (Other), rrk1:sgRNA (Other)	Yeast Expression, CRISPR	Zaratiegui	Implementation of the CRISPR-Cas9 system in fission yeast. Nat Commun. 2014 Oct 29;5:5344. doi: 10.1038/ncomms6344.
	pX330 Pten	sgRNA targeting Pten (Mus musculus)	Mammalian Expression, CRISPR	Jacks	CRISPR-mediated direct mutation of cancer genes in the mouse liver. Nature. 2014 Aug 6. doi: 10.1038/nature13589.
	pX330 p53	sgRNA targeting p53 (Mus musculus)	Mammalian Expression, CRISPR	Jacks	CRISPR-mediated direct mutation of cancer genes in the mouse liver. Nature. 2014 Aug 6. doi: 10.1038/nature13589.
	pX330 Ctnnb1.1	sgRNA targeting Ctnnb1 (Mus musculus)	Mammalian Expression, CRISPR	Jacks	CRISPR-mediated direct mutation of cancer genes in the mouse liver. Nature. 2014 Aug 6. doi: 10.1038/nature13589.
	pX330 Ctnnb1.2	Ctnnb1 (Mus musculus)	Mammalian Expression, CRISPR	Jacks	CRISPR-mediated direct mutation of cancer genes in the mouse liver. Nature. 2014 Aug 6. doi: 10.1038/nature13589.
	pJA45	rde-1(D718) gRNA (Caenorhabditis elegans)	CRISPR	Fire	Efficient Marker-Free Recovery of Custom Genetic Modifications with CRISPR/Cas9 in Caenorhabditis elegans. Genetics. 2014 Aug 26. pii: genetics.114.169730.
	pJA46	rde-1(D801) gRNA (Caenorhabditis elegans)	CRISPR	Fire	Efficient Marker-Free Recovery of Custom Genetic Modifications with CRISPR/Cas9 in Caenorhabditis elegans. Genetics. 2014 Aug 26. pii: genetics.114.169730.
	pJA14	rde-1(H974) gRNA (Caenorhabditis elegans)	CRISPR	Fire	Efficient Marker-Free Recovery of Custom Genetic Modifications with CRISPR/Cas9 in Caenorhabditis elegans. Genetics. 2014 Aug 26. pii: genetics.114.169730.
	pJA42	rol-6(su1006) gRNA (Caenorhabditis elegans)	CRISPR	Fire	Efficient Marker-Free Recovery of Custom Genetic Modifications with CRISPR/Cas9 in Caenorhabditis elegans. Genetics. 2014 Aug 26. pii: genetics.114.169730.
	pJA50	unc-58(e665) gRNA (Caenorhabditis elegans)	CRISPR	Fire	Efficient Marker-Free Recovery of Custom Genetic Modifications with CRISPR/Cas9 in Caenorhabditis elegans. Genetics. 2014 Aug 26. pii: genetics.114.169730.
	pJA59	unc-109(n499) gRNA (Caenorhabditis elegans)	CRISPR	Fire	Efficient Marker-Free Recovery of Custom Genetic Modifications with CRISPR/Cas9 in Caenorhabditis elegans. Genetics. 2014 Aug 26. pii: genetics.114.169730.
	pJA58	dpy-10(cn64) gRNA (Caenorhabditis elegans)	CRISPR	Fire	Efficient Marker-Free Recovery of Custom Genetic Modifications with CRISPR/Cas9 in Caenorhabditis elegans. Genetics. 2014 Aug 26. pii: genetics.114.169730.
	pJA55	sqt-1(e1350) gRNA2 (Caenorhabditis elegans)	CRISPR	Fire	Efficient Marker-Free Recovery of Custom Genetic Modifications with CRISPR/Cas9 in Caenorhabditis elegans. Genetics. 2014 Aug 26. pii: genetics.114.169730.
	U6>Ebf.774(F+E)	Ebf.774 (F+E) sgRNA	CRISPR	Christiaen	Tissue-specific genome editing in Ciona embryos by CRISPR/Cas9. Development. 2014 Nov;141(21):4115-20. doi: 10.1242/dev.114488.
	U6>Control(F+E)	Control (F+E) sgRNA	CRISPR	Christiaen	Tissue-specific genome editing in Ciona embryos by CRISPR/Cas9. Development. 2014 Nov;141(21):4115-20. doi: 10.1242/dev.114488.
	RFN_EGFP_47	pair of multiplex gRNAs targeting EGFP	Mammalian Expression, CRISPR	Joung	Dimeric CRISPR RNA-guided FokI nucleases for highly specific genome editing. Nat Biotechnol. 2014 Apr 25. doi: 10.1038/nbt.2908.
	RFN_EGFP_80	pair of multiplex gRNAs targeting EGFP	Mammalian Expression, CRISPR	Joung	Dimeric CRISPR RNA-guided FokI nucleases for highly specific genome editing. Nat Biotechnol. 2014 Apr 25. doi: 10.1038/nbt.2908.
	RFN_EGFP_81	pair of multiplex gRNAs targeting EGFP	Mammalian Expression, CRISPR	Joung	Dimeric CRISPR RNA-guided FokI nucleases for highly specific genome editing. Nat Biotechnol. 2014 Apr 25. doi: 10.1038/nbt.2908.
	RFN_EGFP_82	pair of multiplex gRNAs targeting EGFP	Mammalian Expression, CRISPR	Joung	Dimeric CRISPR RNA-guided FokI nucleases for highly specific genome editing. Nat Biotechnol. 2014 Apr 25. doi: 10.1038/nbt.2908.
	RFN_EGFP_83	pair of multiplex gRNAs targeting EGFP	Mammalian Expression, CRISPR	Joung	Dimeric CRISPR RNA-guided FokI nucleases for highly specific genome editing. Nat Biotechnol. 2014 Apr 25. doi: 10.1038/nbt.2908.
	RFN_EGFP_84	pair of multiplex gRNAs targeting EGFP	Mammalian Expression, CRISPR	Joung	Dimeric CRISPR RNA-guided FokI nucleases for highly specific genome editing. Nat Biotechnol. 2014 Apr 25. doi: 10.1038/nbt.2908.
	pJA54	sqt-1(e1350) gRNA1 (Caenorhabditis elegans)	CRISPR	Fire	Efficient Marker-Free Recovery of Custom Genetic Modifications with CRISPR/Cas9 in Caenorhabditis elegans. Genetics. 2014 Aug 26. pii: genetics.114.169730.
	AAV:ITR-U6-sgRNA(Kras)-U6-sgRNA(p53)-U6-sgRNA(Lkb1)-pEFS-Rluc-2A-Cre-shortPA-KrasG12D_HDRdonor-ITR (AAV-KPL)	sgRNA (Synthetic), Renilla luciferase, Cre recombinase, KrasG12D HDR donor (Mus musculus)	Mammalian Expression, Mouse Targeting, AAV, Cre/Lox, CRISPR	Zhang	CRISPR-Cas9 Knockin Mice for Genome Editing and Cancer Modeling. Cell. 2014 Sep 24. pii: S0092-8674(14)01163-5. doi: 10.1016/j.cell.2014.09.014.
	AAV:ITR-U6-sgRNA(LacZ)-pEFS-Rluc-2A-Cre-WPRE-hGHpA-ITR	sgRNA, Renilla luciferase, Cre recombinase	Mammalian Expression, Mouse Targeting, AAV, Cre/Lox, CRISPR, Luciferase	Zhang	CRISPR-Cas9 Knockin Mice for Genome Editing and Cancer Modeling. Cell. 2014 Sep 24. pii: S0092-8674(14)01163-5. doi: 10.1016/j.cell.2014.09.014.
	AAV:ITR-U6-sgRNA(NeuN)-pCBh-Cre-WPRE-hGHpA-ITR	sgRNA, Cre recombinase	Mammalian Expression, Mouse Targeting, AAV, Cre/Lox, CRISPR	Zhang	CRISPR-Cas9 Knockin Mice for Genome Editing and Cancer Modeling. Cell. 2014 Sep 24. pii: S0092-8674(14)01163-5. doi: 10.1016/j.cell.2014.09.014.
	AAV:ITR-U6-sgRNA(LacZ)-pCBh-Cre-WPRE-hGHpA-ITR	sgRNA, Cre recombinase	Mammalian Expression, Mouse Targeting, AAV, Cre/Lox, CRISPR	Zhang	CRISPR-Cas9 Knockin Mice for Genome Editing and Cancer Modeling. Cell. 2014 Sep 24. pii: S0092-8674(14)01163-5. doi: 10.1016/j.cell.2014.09.014.
	AAV:ITR-U6-sgRNA(NeuN)-hSyn-Cre-2A-EGFP-KASH-WPRE-shortPA-ITR	sgRNA, Cre recombinase, EGFP	Mammalian Expression, Mouse Targeting, AAV, Cre/Lox, CRISPR	Zhang	CRISPR-Cas9 Knockin Mice for Genome Editing and Cancer Modeling. Cell. 2014 Sep 24. pii: S0092-8674(14)01163-5. doi: 10.1016/j.cell.2014.09.014.
	pGL3-Basic-8x-gRNA-eGFP	8 copies of gRNA binding site (5'-AAAGGTCGAGAAACTGCAAA-3')		Gersbach	A light-inducible CRISPR-Cas9 system for control of endogenous gene activation. Nat Chem Biol. 2015 Feb 9. doi: 10.1038/nchembio.1753.
	gRNA-eGFP-Reporter	gRNA (5'-AAAGGTCGAGAAACTGCAAA-3')	Mammalian Expression, CRISPR	Gersbach	A light-inducible CRISPR-Cas9 system for control of endogenous gene activation. Nat Chem Biol. 2015 Feb 9. doi: 10.1038/nchembio.1753.
	pCAS	S. pyogenese Cas9 (Other), RNA pol III promoter (tRNA-Tyr) (Saccharomyces cerevisiae), hepatitis delta virus ribozyme, genomic (Other), sgRNA (Synthetic)	Bacterial Expression, Yeast Expression, CRISPR, Synthetic Biology	Cate	Selection of chromosomal DNA libraries using a multiplex CRISPR system. Elife. 2014 Aug 19;3. doi: 10.7554/eLife.03703.
	pLV-sgCDKN1B#2 BFP	BFP	Mammalian Expression, Lentiviral, CRISPR	Vale	A Protein-Tagging System for Signal Amplification in Gene Expression and Fluorescence Imaging. Cell. 2014 Oct 8. pii: S0092-8674(14)01227-6. doi: 10.1016/j.cell.2014.09.039.
	pT7-gRNA:Tyr (albino)	Tyrosinase gRNA (Rattus norvegicus)	CRISPR	Mashimo	Allele-specific genome editing and correction of disease-associated phenotypes in rats using the CRISPR-Cas platform. Nat Commun. 2014 Jun 26;5:4240. doi: 10.1038/ncomms5240.
	pT7-gRNA:Tyr (wild)	Tyrosinase gRNA (Rattus norvegicus)	CRISPR	Mashimo	Allele-specific genome editing and correction of disease-associated phenotypes in rats using the CRISPR-Cas platform. Nat Commun. 2014 Jun 26;5:4240. doi: 10.1038/ncomms5240.
	pT7-gRNA:Asip	Agouti signaling protein gRNA (Rattus norvegicus)	CRISPR	Mashimo	Allele-specific genome editing and correction of disease-associated phenotypes in rats using the CRISPR-Cas platform. Nat Commun. 2014 Jun 26;5:4240. doi: 10.1038/ncomms5240.
	pT7-gRNA:kit-1	v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog gRNA (Rattus norvegicus)	CRISPR	Mashimo	Allele-specific genome editing and correction of disease-associated phenotypes in rats using the CRISPR-Cas platform. Nat Commun. 2014 Jun 26;5:4240. doi: 10.1038/ncomms5240.
	pT7-gRNA:kit-2-1	v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog gRNA (Rattus norvegicus)	CRISPR	Mashimo	Allele-specific genome editing and correction of disease-associated phenotypes in rats using the CRISPR-Cas platform. Nat Commun. 2014 Jun 26;5:4240. doi: 10.1038/ncomms5240.
	pT7-gRNA:kit-2-2	v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog gRNA (Rattus norvegicus)	CRISPR	Mashimo	Allele-specific genome editing and correction of disease-associated phenotypes in rats using the CRISPR-Cas platform. Nat Commun. 2014 Jun 26;5:4240. doi: 10.1038/ncomms5240.
	DR274-eGFP sgRNA	EGFP sgRNA (Synthetic)	CRISPR	Del Bene	Highly efficient CRISPR/Cas9-mediated knock-in in zebrafish by homology-independent DNA repair. Genome Res. 2014 Jan;24(1):142-53. doi: 10.1101/gr.161638.113. Epub 2013 Oct 31.
	gRNA-hIRF-1 #12	gRNA_hIRF1 promoter #12 (Homo sapiens)	Mammalian Expression, CRISPR	Fujii	Efficient isolation of specific genomic regions and identification of associated proteins by engineered DNA-binding molecule-mediated chromatin immunoprecipitation (enChIP) using CRISPR. Biochem Biophys Res Commun. 2013 Sep 13;439(1):132-6. doi: 10.1016/j.bbrc.2013.08.013. Epub 2013 Aug 11.
	gRNA-hIRF-1 #12/pSIR	gRNA_hIRF1 promoter #12 (Homo sapiens)	Mammalian Expression, Retroviral, CRISPR	Fujii	Identification of Proteins Associated with an IFNgamma-Responsive Promoter by a Retroviral Expression System for enChIP Using CRISPR. PLoS One. 2014 Jul 22;9(7):e103084. doi: 10.1371/journal.pone.0103084. eCollection 2014.
	pJW1219	sgRNA(F+E) (Synthetic)	Worm Expression, CRISPR	Ward	Rapid and Precise Engineering of the Caenorhabditis elegans Genome with Lethal Mutation Co-conversion and Inactivation of NHEJ Repair. Genetics. 2014 Dec 9. pii: genetics.114.172361.
	pJW1285	sgRNA(F+E) targeting pha-1 (Synthetic)	Worm Expression, CRISPR	Ward	Rapid and Precise Engineering of the Caenorhabditis elegans Genome with Lethal Mutation Co-conversion and Inactivation of NHEJ Repair. Genetics. 2014 Dec 9. pii: genetics.114.172361.
	pMM178	Cas9, tracrRNA, crRNA (Other)	Bacterial Expression, CRISPR	Lu	Sequence-specific antimicrobials using efficiently delivered RNA-guided nucleases. Nat Biotechnol. 2014 Sep 21. doi: 10.1038/nbt.3011.
	pMM441	Cas9, tracrRNA, crRNA (Other)	Bacterial Expression, CRISPR	Lu	Sequence-specific antimicrobials using efficiently delivered RNA-guided nucleases. Nat Biotechnol. 2014 Sep 21. doi: 10.1038/nbt.3011.
	pRC319	Cas9, tracrRNA, crRNA (Other)	Bacterial Expression, CRISPR	Lu	Sequence-specific antimicrobials using efficiently delivered RNA-guided nucleases. Nat Biotechnol. 2014 Sep 21. doi: 10.1038/nbt.3011.
	px335 Mettl14 sgRNA #2	gccgctcccggatctcctgc (Mus musculus)	Mammalian Expression, CRISPR	Hanna	m6A mRNA methylation facilitates resolution of naïve pluripotency toward differentiation. Science 1 January 2015
	px335 Mettl14 sgRNA #1	gcggcagctcctagctcagc (Mus musculus)	Mammalian Expression, CRISPR	Hanna	m6A mRNA methylation facilitates resolution of naïve pluripotency toward differentiation. Science 1 January 2015
	px330-USP13	USP13 (Homo sapiens)	CRISPR	Ye	USP13 antagonizes gp78 to maintain functionality of a chaperone in ER-associated degradation. Elife. 2014;3:e01369. doi: 10.7554/eLife.01369. Epub 2014 Jan 14.
	px330-gp78	gp78 (Homo sapiens)	CRISPR	Ye	USP13 antagonizes gp78 to maintain functionality of a chaperone in ER-associated degradation. Elife. 2014;3:e01369. doi: 10.7554/eLife.01369. Epub 2014 Jan 14.
	MLM3636-Prnp-CDS-Neg	PrP guiding sequence (Mus musculus)	Mammalian Expression, CRISPR	Schmitt-Ulms	CRISPR-Cas9-Based Knockout of the Prion Protein and Its Effect on the Proteome. PLoS One. 2014 Dec 9;9(12):e114594. doi: 10.1371/journal.pone.0114594. eCollection 2014.
	MLM3636-Prnp-CDS-Pos	PrP guiding sequence (Mus musculus)	Mammalian Expression, CRISPR	Schmitt-Ulms	CRISPR-Cas9-Based Knockout of the Prion Protein and Its Effect on the Proteome. PLoS One. 2014 Dec 9;9(12):e114594. doi: 10.1371/journal.pone.0114594. eCollection 2014.
	pTargetF	sgRNA (Synthetic)	Bacterial Expression, CRISPR	Yang	Multigene editing in the Escherichia coli genome using the CRISPR-Cas9 system. Appl Environ Microbiol. 2015 Jan 30. pii: AEM.04023-14.
	pAN-PBAD-sgRNA-A1NT	A1NT (Synthetic)	Bacterial Expression, CRISPR	Voigt	Multi-input CRISPR/Cas genetic circuits that interface host regulatory networks. Mol Syst Biol. 2014 Nov 24;10:763. doi: 10.15252/msb.20145735.
	pAN-PBAD-sgRNA-A1T	A1T sgRNA (Synthetic)	Bacterial Expression, CRISPR	Voigt	Multi-input CRISPR/Cas genetic circuits that interface host regulatory networks. Mol Syst Biol. 2014 Nov 24;10:763. doi: 10.15252/msb.20145735.
	pAN-PBAD-sgRNA-A2NT	A2NT sgRNA	Bacterial Expression, CRISPR	Voigt	Multi-input CRISPR/Cas genetic circuits that interface host regulatory networks. Mol Syst Biol. 2014 Nov 24;10:763. doi: 10.15252/msb.20145735.
	pAN-PBAD-sgRNA-A2T	A2T sgRNA (Synthetic)	Bacterial Expression, CRISPR	Voigt	Multi-input CRISPR/Cas genetic circuits that interface host regulatory networks. Mol Syst Biol. 2014 Nov 24;10:763. doi: 10.15252/msb.20145735.
	pAN-PBAD-sgRNA-A3NT	A3NT sgRNA (Synthetic)	Bacterial Expression, CRISPR	Voigt	Multi-input CRISPR/Cas genetic circuits that interface host regulatory networks. Mol Syst Biol. 2014 Nov 24;10:763. doi: 10.15252/msb.20145735.
	pAN-PBAD-sgRNA-A3T	A3T sgRNA (Synthetic)	Bacterial Expression, CRISPR	Voigt	Multi-input CRISPR/Cas genetic circuits that interface host regulatory networks. Mol Syst Biol. 2014 Nov 24;10:763. doi: 10.15252/msb.20145735.
	pAN-PBAD-sgRNA-A4NT	A4NT sgRNA (Synthetic)	Bacterial Expression, CRISPR	Voigt	Multi-input CRISPR/Cas genetic circuits that interface host regulatory networks. Mol Syst Biol. 2014 Nov 24;10:763. doi: 10.15252/msb.20145735.
	pAN-PBAD-sgRNA-A4T	A4T sgRNA (Synthetic)	Bacterial Expression, CRISPR	Voigt	Multi-input CRISPR/Cas genetic circuits that interface host regulatory networks. Mol Syst Biol. 2014 Nov 24;10:763. doi: 10.15252/msb.20145735.
	pAN-PBAD-sgRNA-A5NT	A5NT sgRNA (Synthetic)	Bacterial Expression, CRISPR	Voigt	Multi-input CRISPR/Cas genetic circuits that interface host regulatory networks. Mol Syst Biol. 2014 Nov 24;10:763. doi: 10.15252/msb.20145735.
	pAN-PBAD-sgRNA-A5T	A5T sgRNA (Synthetic)	Bacterial Expression, CRISPR	Voigt	Multi-input CRISPR/Cas genetic circuits that interface host regulatory networks. Mol Syst Biol. 2014 Nov 24;10:763. doi: 10.15252/msb.20145735.
	pAN-PBAD-sgRNA-scramble	scramble sgRNA (Synthetic)	Bacterial Expression, CRISPR	Voigt	Multi-input CRISPR/Cas genetic circuits that interface host regulatory networks. Mol Syst Biol. 2014 Nov 24;10:763. doi: 10.15252/msb.20145735.
	pAN-PBAD-sgRNA-VR	VR sgRNA (Synthetic)	Bacterial Expression, CRISPR	Voigt	Multi-input CRISPR/Cas genetic circuits that interface host regulatory networks. Mol Syst Biol. 2014 Nov 24;10:763. doi: 10.15252/msb.20145735.
	pAN-NOR	A2NT sgRNA (Synthetic), A2NT sgRNA (Synthetic)	Bacterial Expression, CRISPR	Voigt	Multi-input CRISPR/Cas genetic circuits that interface host regulatory networks. Mol Syst Biol. 2014 Nov 24;10:763. doi: 10.15252/msb.20145735.
	pAN-AND	A1NT sgRNA (Synthetic), A4NT sgRNA (Synthetic), A2NT sgRNA (Synthetic), A1NT (Synthetic)	Bacterial Expression, CRISPR	Voigt	Multi-input CRISPR/Cas genetic circuits that interface host regulatory networks. Mol Syst Biol. 2014 Nov 24;10:763. doi: 10.15252/msb.20145735.
	pAN-OR-MalT-3NT	A2NT sgRNA (Synthetic), Malt-3NT sgRNA (Synthetic), A2NT sgRNA (Synthetic)	Bacterial Expression, CRISPR	Voigt	Multi-input CRISPR/Cas genetic circuits that interface host regulatory networks. Mol Syst Biol. 2014 Nov 24;10:763. doi: 10.15252/msb.20145735.
	pJZC545	sgRNA + 1x MS2 binding module	Yeast Expression	Qi	Engineering Complex Synthetic Transcriptional Programs with CRISPR RNA Scaffolds. Cell. 2014 Dec 18. pii: S0092-8674(14)01570-0. doi: 10.1016/j.cell.2014.11.052.
	pJZC583	sgRNA + 2x MS2 binding module	Yeast Expression	Qi	Engineering Complex Synthetic Transcriptional Programs with CRISPR RNA Scaffolds. Cell. 2014 Dec 18. pii: S0092-8674(14)01570-0. doi: 10.1016/j.cell.2014.11.052.
	pJZC588	sgRNA + 2x MS2 (wt+f6) binding module	Yeast Expression	Qi	Engineering Complex Synthetic Transcriptional Programs with CRISPR RNA Scaffolds. Cell. 2014 Dec 18. pii: S0092-8674(14)01570-0. doi: 10.1016/j.cell.2014.11.052.
	pJZC548	sgRNA + 1x PP7	Yeast Expression	Qi	Engineering Complex Synthetic Transcriptional Programs with CRISPR RNA Scaffolds. Cell. 2014 Dec 18. pii: S0092-8674(14)01570-0. doi: 10.1016/j.cell.2014.11.052.
	pJZC603	sgRNA + 2x PP7 RNA binding module	Yeast Expression	Qi	Engineering Complex Synthetic Transcriptional Programs with CRISPR RNA Scaffolds. Cell. 2014 Dec 18. pii: S0092-8674(14)01570-0. doi: 10.1016/j.cell.2014.11.052.
	pJZC572	sgRNA + 1x com RNA binding module	Yeast Expression	Qi	Engineering Complex Synthetic Transcriptional Programs with CRISPR RNA Scaffolds. Cell. 2014 Dec 18. pii: S0092-8674(14)01570-0. doi: 10.1016/j.cell.2014.11.052.
	pJZC593	sgRNA + MS2-PP7 RNA binding module	Yeast Expression	Qi	Engineering Complex Synthetic Transcriptional Programs with CRISPR RNA Scaffolds. Cell. 2014 Dec 18. pii: S0092-8674(14)01570-0. doi: 10.1016/j.cell.2014.11.052.
	pJZC523	sgRNA	Yeast Expression	Qi	Engineering Complex Synthetic Transcriptional Programs with CRISPR RNA Scaffolds. Cell. 2014 Dec 18. pii: S0092-8674(14)01570-0. doi: 10.1016/j.cell.2014.11.052.
	pJZC625	sgRNA +1x MS2, Pol II promoter with ribozyme cleavage	Yeast Expression	Qi	Engineering Complex Synthetic Transcriptional Programs with CRISPR RNA Scaffolds. Cell. 2014 Dec 18. pii: S0092-8674(14)01570-0. doi: 10.1016/j.cell.2014.11.052.
	pJZC35	sgRNA	Mammalian Expression, Lentiviral	Qi	Engineering Complex Synthetic Transcriptional Programs with CRISPR RNA Scaffolds. Cell. 2014 Dec 18. pii: S0092-8674(14)01570-0. doi: 10.1016/j.cell.2014.11.052.
	pJZC32	sgRNA, MCP-VP64	Mammalian Expression, Lentiviral	Qi	Engineering Complex Synthetic Transcriptional Programs with CRISPR RNA Scaffolds. Cell. 2014 Dec 18. pii: S0092-8674(14)01570-0. doi: 10.1016/j.cell.2014.11.052.
	pJZC25	sgRNA + 1x MS2 binding module, MCP-VP64	Mammalian Expression, Lentiviral	Qi	Engineering Complex Synthetic Transcriptional Programs with CRISPR RNA Scaffolds. Cell. 2014 Dec 18. pii: S0092-8674(14)01570-0. doi: 10.1016/j.cell.2014.11.052.
	pJZC33	sgRNA + 2x MS2 binding module, MCP-VP64	Mammalian Expression, Lentiviral	Qi	Engineering Complex Synthetic Transcriptional Programs with CRISPR RNA Scaffolds. Cell. 2014 Dec 18. pii: S0092-8674(14)01570-0. doi: 10.1016/j.cell.2014.11.052.
	pJZC34	sgRNA + 2x MS2(wt+f6) binding module, MCP-VP64	Mammalian Expression, Lentiviral	Qi	Engineering Complex Synthetic Transcriptional Programs with CRISPR RNA Scaffolds. Cell. 2014 Dec 18. pii: S0092-8674(14)01570-0. doi: 10.1016/j.cell.2014.11.052.
	pJZC41	sgRNA, PCP-VP64	Mammalian Expression, Lentiviral	Qi	Engineering Complex Synthetic Transcriptional Programs with CRISPR RNA Scaffolds. Cell. 2014 Dec 18. pii: S0092-8674(14)01570-0. doi: 10.1016/j.cell.2014.11.052.
	pJZC39	sgRNA + 1x PP7, mCherry	Mammalian Expression, Lentiviral	Qi	Engineering Complex Synthetic Transcriptional Programs with CRISPR RNA Scaffolds. Cell. 2014 Dec 18. pii: S0092-8674(14)01570-0. doi: 10.1016/j.cell.2014.11.052.
	pJZC40	sgRNA + 2x PP7, mCherry	Mammalian Expression, Lentiviral	Qi	Engineering Complex Synthetic Transcriptional Programs with CRISPR RNA Scaffolds. Cell. 2014 Dec 18. pii: S0092-8674(14)01570-0. doi: 10.1016/j.cell.2014.11.052.
	pJZC101	sgRNA, COM-VP64	Mammalian Expression, Lentiviral	Qi	Engineering Complex Synthetic Transcriptional Programs with CRISPR RNA Scaffolds. Cell. 2014 Dec 18. pii: S0092-8674(14)01570-0. doi: 10.1016/j.cell.2014.11.052.
	pJZC48	sgRNA + 1x COM binding module, COM-VP64	Mammalian Expression, Lentiviral	Qi	Engineering Complex Synthetic Transcriptional Programs with CRISPR RNA Scaffolds. Cell. 2014 Dec 18. pii: S0092-8674(14)01570-0. doi: 10.1016/j.cell.2014.11.052.
	pJZC102	sgRNA	Mammalian Expression, Lentiviral	Qi	Engineering Complex Synthetic Transcriptional Programs with CRISPR RNA Scaffolds. Cell. 2014 Dec 18. pii: S0092-8674(14)01570-0. doi: 10.1016/j.cell.2014.11.052.
	pJZC77	sgRNA, COM-KRAB	Mammalian Expression, Lentiviral	Qi	Engineering Complex Synthetic Transcriptional Programs with CRISPR RNA Scaffolds. Cell. 2014 Dec 18. pii: S0092-8674(14)01570-0. doi: 10.1016/j.cell.2014.11.052.
	pJZC78	sgRNA + 1x COM binding module, COM-KRAB	Mammalian Expression, Lentiviral	Qi	Engineering Complex Synthetic Transcriptional Programs with CRISPR RNA Scaffolds. Cell. 2014 Dec 18. pii: S0092-8674(14)01570-0. doi: 10.1016/j.cell.2014.11.052.
	pJZC103	sgRNA	Mammalian Expression, Lentiviral	Qi	Engineering Complex Synthetic Transcriptional Programs with CRISPR RNA Scaffolds. Cell. 2014 Dec 18. pii: S0092-8674(14)01570-0. doi: 10.1016/j.cell.2014.11.052.
	pJZC73	sgRNA, COM-KRAB	Mammalian Expression, Lentiviral	Qi	Engineering Complex Synthetic Transcriptional Programs with CRISPR RNA Scaffolds. Cell. 2014 Dec 18. pii: S0092-8674(14)01570-0. doi: 10.1016/j.cell.2014.11.052.
	pJZC74	sgRNA + 1x COM binding module, COM-KRAB	Mammalian Expression, Lentiviral	Qi	Engineering Complex Synthetic Transcriptional Programs with CRISPR RNA Scaffolds. Cell. 2014 Dec 18. pii: S0092-8674(14)01570-0. doi: 10.1016/j.cell.2014.11.052.
	pJZC116	sgRNA + 2x MS2 (wt+f6) binding module, MCP-VP64	Mammalian Expression, Lentiviral	Qi	Engineering Complex Synthetic Transcriptional Programs with CRISPR RNA Scaffolds. Cell. 2014 Dec 18. pii: S0092-8674(14)01570-0. doi: 10.1016/j.cell.2014.11.052.
	pKCcas9dO	Scocas9 (Other), sgRNA (), act-orf4 homology arm	Bacterial Expression, CRISPR ; Bacterial Genome Editing	Jiang	One-step high-efficiency CRISPR/Cas9-mediated genome editing in Streptomyces. Acta Biochim Biophys Sin (Shanghai). 2015 Apr;47(4):231-43. doi: 10.1093/abbs/gmv007. Epub 2015 Mar 3.
	LentiCRISPR_Hs_MBD3_ex2	MBD3 gRNA (Homo sapiens)	Lentiviral, CRISPR	Wade	LentiCRISPR_Hs_MBD3_ex2 (unpublished)
	LentiCRISPR_Hs_MBD3_ex3	MBD3 gRNA (Homo sapiens)	Lentiviral, CRISPR	Wade	LentiCRISPR_Hs_MBD3_ex2 (unpublished)
	ptreCas9-mKate2ps-T1gRNA	Cas9–mKate2ps (Synthetic)	Mammalian Expression	Bleris	CRISPR-based self-cleaving mechanism for controllable gene delivery in human cells. Nucleic Acids Res. 2015 Jan 30;43(2):1297-303. doi: 10.1093/nar/gku1326. Epub 2014 Dec 18.
	pCas9–mKate2ps–T1gRNA	Cas9–mKate2ps (Synthetic)	Mammalian Expression	Bleris	CRISPR-based self-cleaving mechanism for controllable gene delivery in human cells. Nucleic Acids Res. 2015 Jan 30;43(2):1297-303. doi: 10.1093/nar/gku1326. Epub 2014 Dec 18.
	pX330_sgACTA2	ACTA2 (Homo sapiens)	Mammalian Expression	Qi	Small Molecules Enhance CRISPR Genome Editing in Pluripotent Stem Cells. Cell Stem Cell. 2015 Feb 5;16(2):142-7. doi: 10.1016/j.stem.2015.01.003.
	PX458_CEBPB_1	gRNA	CRISPR	Mendenhall	CETCh-seq: CRISPR epitope tagging ChIP-seq of DNA-binding proteins. Genome Res. 2015 Sep 9.
	PX458_CEBPB_2	gRNA	CRISPR	Mendenhall	CETCh-seq: CRISPR epitope tagging ChIP-seq of DNA-binding proteins. Genome Res. 2015 Sep 9.
	PX458_RAD21_1	gRNA	CRISPR	Mendenhall	CETCh-seq: CRISPR epitope tagging ChIP-seq of DNA-binding proteins. Genome Res. 2015 Sep 9.
	Adeno EA	U6_sgRNA(Alk)_U6_sgRNA(Eml4)_CBh_FLAG-Cas9 (Mus musculus)	Adenoviral	Ventura	In vivo engineering of oncogenic chromosomal rearrangements with the CRISPR/Cas9 system. Nature. 2014 Dec 18;516(7531):423-7. doi: 10.1038/nature13902. Epub 2014 Oct 22.
	sgRNA1_ASCL1	sgRNA1_ASCL1 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Sato	CRISPR-Cas9-based Photoactivatable Transcription System. Chem Biol. 2015 Feb 19;22(2):169-74. doi: 10.1016/j.chembiol.2014.12.011. Epub 2015 Jan 22.
	sgRNA2_ASCL1	sgRNA1_ASCL2 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Sato	CRISPR-Cas9-based Photoactivatable Transcription System. Chem Biol. 2015 Feb 19;22(2):169-74. doi: 10.1016/j.chembiol.2014.12.011. Epub 2015 Jan 22.
	sgRNA3_ASCL1	sgRNA1_ASCL3 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Sato	CRISPR-Cas9-based Photoactivatable Transcription System. Chem Biol. 2015 Feb 19;22(2):169-74. doi: 10.1016/j.chembiol.2014.12.011. Epub 2015 Jan 22.
	sgRNA4_ASCL1	sgRNA1_ASCL4 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Sato	CRISPR-Cas9-based Photoactivatable Transcription System. Chem Biol. 2015 Feb 19;22(2):169-74. doi: 10.1016/j.chembiol.2014.12.011. Epub 2015 Jan 22.
	sgRNA1_MYOD1	sgRNA1_MYOD1 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Sato	CRISPR-Cas9-based Photoactivatable Transcription System. Chem Biol. 2015 Feb 19;22(2):169-74. doi: 10.1016/j.chembiol.2014.12.011. Epub 2015 Jan 22.
	sgRNA2_MYOD1	sgRNA1_MYOD1 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Sato	CRISPR-Cas9-based Photoactivatable Transcription System. Chem Biol. 2015 Feb 19;22(2):169-74. doi: 10.1016/j.chembiol.2014.12.011. Epub 2015 Jan 22.
	sgRNA3_MYOD1	sgRNA1_MYOD1 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Sato	CRISPR-Cas9-based Photoactivatable Transcription System. Chem Biol. 2015 Feb 19;22(2):169-74. doi: 10.1016/j.chembiol.2014.12.011. Epub 2015 Jan 22.
	sgRNA4_MYOD1	sgRNA1_MYOD1 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Sato	CRISPR-Cas9-based Photoactivatable Transcription System. Chem Biol. 2015 Feb 19;22(2):169-74. doi: 10.1016/j.chembiol.2014.12.011. Epub 2015 Jan 22.
	sgRNA1_IL1RN	IL1RN (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Sato	CRISPR-Cas9-based Photoactivatable Transcription System. Chem Biol. 2015 Feb 19;22(2):169-74. doi: 10.1016/j.chembiol.2014.12.011. Epub 2015 Jan 22.
	sgRNA2_IL1RN	sgRNA2_IL1RN (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Sato	CRISPR-Cas9-based Photoactivatable Transcription System. Chem Biol. 2015 Feb 19;22(2):169-74. doi: 10.1016/j.chembiol.2014.12.011. Epub 2015 Jan 22.
	sgRNA3_IL1RN	sgRNA3_IL1RN (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Sato	CRISPR-Cas9-based Photoactivatable Transcription System. Chem Biol. 2015 Feb 19;22(2):169-74. doi: 10.1016/j.chembiol.2014.12.011. Epub 2015 Jan 22.
	sgRNA4_IL1RN	sgRNA4_IL1RN (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Sato	CRISPR-Cas9-based Photoactivatable Transcription System. Chem Biol. 2015 Feb 19;22(2):169-74. doi: 10.1016/j.chembiol.2014.12.011. Epub 2015 Jan 22.
	sgRNA1_NANOG	sgRNA1_NANOG (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Sato	CRISPR-Cas9-based Photoactivatable Transcription System. Chem Biol. 2015 Feb 19;22(2):169-74. doi: 10.1016/j.chembiol.2014.12.011. Epub 2015 Jan 22.
	sgRNA2_NANOG	sgRNA2_NANOG (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Sato	CRISPR-Cas9-based Photoactivatable Transcription System. Chem Biol. 2015 Feb 19;22(2):169-74. doi: 10.1016/j.chembiol.2014.12.011. Epub 2015 Jan 22.
	sgRNA3_NANOG	sgRNA3_NANOG (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Sato	CRISPR-Cas9-based Photoactivatable Transcription System. Chem Biol. 2015 Feb 19;22(2):169-74. doi: 10.1016/j.chembiol.2014.12.011. Epub 2015 Jan 22.
	sgRNA4_NANOG	sgRNA4_NANOG (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Sato	CRISPR-Cas9-based Photoactivatable Transcription System. Chem Biol. 2015 Feb 19;22(2):169-74. doi: 10.1016/j.chembiol.2014.12.011. Epub 2015 Jan 22.
	sgRNA1_GAL4UAS-Luciferase reporter	sgRNA1 for GAL4UAS-Luciferase reporter (Synthetic)	Mammalian Expression, Lentiviral, CRISPR	Sato	CRISPR-Cas9-based Photoactivatable Transcription System. Chem Biol. 2015 Feb 19;22(2):169-74. doi: 10.1016/j.chembiol.2014.12.011. Epub 2015 Jan 22.
	sgRNA2_GAL4UAS-Luciferase reporter	sgRNA2 for GAL4UAS-Luciferase reporter (Synthetic)	Mammalian Expression, Lentiviral, CRISPR	Sato	CRISPR-Cas9-based Photoactivatable Transcription System. Chem Biol. 2015 Feb 19;22(2):169-74. doi: 10.1016/j.chembiol.2014.12.011. Epub 2015 Jan 22.
	sgRNA3_GAL4UAS-Luciferase reporter	sgRNA3 for GAL4UAS-Luciferase reporter (Synthetic)	Mammalian Expression, Lentiviral, CRISPR	Sato	CRISPR-Cas9-based Photoactivatable Transcription System. Chem Biol. 2015 Feb 19;22(2):169-74. doi: 10.1016/j.chembiol.2014.12.011. Epub 2015 Jan 22.
	sgRNA1_pNeurog2-Luciferase reporter	sgRNA1 for pNeurog2-Luciferase reporter (Synthetic)	Mammalian Expression, CRISPR	Sato	CRISPR-Cas9-based Photoactivatable Transcription System. Chem Biol. 2015 Feb 19;22(2):169-74. doi: 10.1016/j.chembiol.2014.12.011. Epub 2015 Jan 22.
	sgRNA1_Tet-inducible Luciferase reporter	sgRNA1 for Tet-inducible Luciferase reporter (Synthetic)	Mammalian Expression, CRISPR	Sato	CRISPR-Cas9-based Photoactivatable Transcription System. Chem Biol. 2015 Feb 19;22(2):169-74. doi: 10.1016/j.chembiol.2014.12.011. Epub 2015 Jan 22.
	pU6-sgRosa26-1_CBh-Cas9-T2A-BFP	Cas9 (Other), sgRNA targeting ROSA26-1	Mammalian Expression, CRISPR	Kuehn	Increasing the efficiency of homology-directed repair for CRISPR-Cas9-induced precise gene editing in mammalian cells. Nat Biotechnol. 2015 Mar 24. doi: 10.1038/nbt.3198.
	pU6-sgRosa26-1_CBh-Cas9-T2A-BFP-P2A-Ad4E1B	Cas9 (Other), sgRNA targeting ROSA26-1	Mammalian Expression, CRISPR	Kuehn	Increasing the efficiency of homology-directed repair for CRISPR-Cas9-induced precise gene editing in mammalian cells. Nat Biotechnol. 2015 Mar 24. doi: 10.1038/nbt.3198.
	pU6a:sgRNA(tyr)	U6a:sgRNA (tyr) (Danio rerio)	CRISPR	Chen	Multiplex Conditional Mutagenesis Using Transgenic Expression of Cas9 and sgRNAs. Genetics. 2015 Apr 8. pii: genetics.115.176917.
	PX458_GABPA_1	gRNA	CRISPR	Mendenhall	CETCh-seq: CRISPR epitope tagging ChIP-seq of DNA-binding proteins. Genome Res. 2015 Sep 9.
	PX458_GABPA_2	gRNA	CRISPR	Mendenhall	CETCh-seq: CRISPR epitope tagging ChIP-seq of DNA-binding proteins. Genome Res. 2015 Sep 9.
	gRNA-ura-HYB	gBlock product of ura3 deletion gRNA cassette (Synthetic)	Yeast Expression	Jin	Construction of a quadruple auxotrophic mutant of an industrial polyploid saccharomyces cerevisiae strain by using RNA-guided Cas9 nuclease. Appl Environ Microbiol. 2014 Dec;80(24):7694-701. doi: 10.1128/AEM.02310-14. Epub 2014 Oct 3.
	gRNA-trp-HYB	gBlock product of trp1 deletion gRNA cassette (Synthetic)	Yeast Expression	Jin	Construction of a quadruple auxotrophic mutant of an industrial polyploid saccharomyces cerevisiae strain by using RNA-guided Cas9 nuclease. Appl Environ Microbiol. 2014 Dec;80(24):7694-701. doi: 10.1128/AEM.02310-14. Epub 2014 Oct 3.
	gRNA-leu-HYB	gBlock product of leu2 deletion gRNA cassette (Synthetic)	Yeast Expression	Jin	Construction of a quadruple auxotrophic mutant of an industrial polyploid saccharomyces cerevisiae strain by using RNA-guided Cas9 nuclease. Appl Environ Microbiol. 2014 Dec;80(24):7694-701. doi: 10.1128/AEM.02310-14. Epub 2014 Oct 3.
	gRNA-his-HYB	gBlock product of his3 deletion gRNA cassette (Synthetic)	Yeast Expression	Jin	Construction of a quadruple auxotrophic mutant of an industrial polyploid saccharomyces cerevisiae strain by using RNA-guided Cas9 nuclease. Appl Environ Microbiol. 2014 Dec;80(24):7694-701. doi: 10.1128/AEM.02310-14. Epub 2014 Oct 3.
	pRPR1_a1gRNA_RPR1t	a1 gRNA (Saccharomyces cerevisiae)	Yeast Expression	Lu	Tunable and Multifunctional Eukaryotic Transcription Factors Based on CRISPR/Cas. ACS Synth Biol. 2013 Sep 11.
	pRPR1_c1gRNA_RPR1t	c1 gRNA (Saccharomyces cerevisiae)	Yeast Expression	Lu	Tunable and Multifunctional Eukaryotic Transcription Factors Based on CRISPR/Cas. ACS Synth Biol. 2013 Sep 11.
	pRPR1_c2gRNA_RPR1t	c2 gRNA (Saccharomyces cerevisiae)	Yeast Expression	Lu	Tunable and Multifunctional Eukaryotic Transcription Factors Based on CRISPR/Cas. ACS Synth Biol. 2013 Sep 11.
	pRPR1_c3gRNA_RPR1t	c3 gRNA (Saccharomyces cerevisiae)	Yeast Expression	Lu	Tunable and Multifunctional Eukaryotic Transcription Factors Based on CRISPR/Cas. ACS Synth Biol. 2013 Sep 11.
	pRPR1_c4gRNA_RPR1t	c4 gRNA (Saccharomyces cerevisiae)	Yeast Expression	Lu	Tunable and Multifunctional Eukaryotic Transcription Factors Based on CRISPR/Cas. ACS Synth Biol. 2013 Sep 11.
	pRPR1_c5gRNA_RPR1t	c5 gRNA (Saccharomyces cerevisiae)	Yeast Expression	Lu	Tunable and Multifunctional Eukaryotic Transcription Factors Based on CRISPR/Cas. ACS Synth Biol. 2013 Sep 11.
	pRPR1_c6gRNA_RPR1t	c6 gRNA (Saccharomyces cerevisiae)	Yeast Expression	Lu	Tunable and Multifunctional Eukaryotic Transcription Factors Based on CRISPR/Cas. ACS Synth Biol. 2013 Sep 11.
	pRPR1_c7gRNA_RPR1t	c7 gRNA (Saccharomyces cerevisiae)	Yeast Expression	Lu	Tunable and Multifunctional Eukaryotic Transcription Factors Based on CRISPR/Cas. ACS Synth Biol. 2013 Sep 11.
	pRPR1_c8gRNA_RPR1t	c8 gRNA (Saccharomyces cerevisiae)	Yeast Expression	Lu	Tunable and Multifunctional Eukaryotic Transcription Factors Based on CRISPR/Cas. ACS Synth Biol. 2013 Sep 11.
	pRPR1_g1gRNA_RPR1t	g1 gRNA (Saccharomyces cerevisiae)	Yeast Expression	Lu	Tunable and Multifunctional Eukaryotic Transcription Factors Based on CRISPR/Cas. ACS Synth Biol. 2013 Sep 11.
	pRPR1_g2gRNA_RPR1t	g2 gRNA (Saccharomyces cerevisiae)	Yeast Expression	Lu	Tunable and Multifunctional Eukaryotic Transcription Factors Based on CRISPR/Cas. ACS Synth Biol. 2013 Sep 11.
	PX458_ATF1_1	gRNA	CRISPR	Mendenhall	CETCh-seq: CRISPR epitope tagging ChIP-seq of DNA-binding proteins. Genome Res. 2015 Sep 9.
	PX458_CREB1_1	gRNA	CRISPR	Mendenhall	CETCh-seq: CRISPR epitope tagging ChIP-seq of DNA-binding proteins. Genome Res. 2015 Sep 9.
	PX458_CREB1_2	gRNA	CRISPR	Mendenhall	CETCh-seq: CRISPR epitope tagging ChIP-seq of DNA-binding proteins. Genome Res. 2015 Sep 9.
	pCas9–mKate2ps–CgRNA	Cas9–mKate2ps	Mammalian Expression	Bleris	CRISPR-based self-cleaving mechanism for controllable gene delivery in human cells. Nucleic Acids Res. 2015 Jan 30;43(2):1297-303. doi: 10.1093/nar/gku1326. Epub 2014 Dec 18.
	pT7-th-sgRNA	th sgRNA (Danio rerio)	in vitro RNA transcription	Du	Intron targeting-mediated and endogenous gene integrity-maintaining knockin in zebrafish using the CRISPR/Cas9 system. Cell Res. 2015 Apr 7. doi: 10.1038/cr.2015.43.
	pT7-gfap-sgRNA	gfap sgRNA (Danio rerio)	in vitro RNA transcription	Du	Intron targeting-mediated and endogenous gene integrity-maintaining knockin in zebrafish using the CRISPR/Cas9 system. Cell Res. 2015 Apr 7. doi: 10.1038/cr.2015.43.
	AP56-5	sgRNA for APa13 (Caenorhabditis elegans)	Worm Expression, CRISPR	Seydoux	Scalable and versatile genome editing using linear DNAs with microhomology to Cas9 Sites in Caenorhabditis elegans. Genetics. 2014 Dec;198(4):1347-56. doi: 10.1534/genetics.114.170423. Epub 2014 Sep 23.
	AP56-6	sgRNA for APa13 (Caenorhabditis elegans)	Worm Expression, CRISPR	Seydoux	Scalable and versatile genome editing using linear DNAs with microhomology to Cas9 Sites in Caenorhabditis elegans. Genetics. 2014 Dec;198(4):1347-56. doi: 10.1534/genetics.114.170423. Epub 2014 Sep 23.
	AP56-7	sgRNA for APa13 (Caenorhabditis elegans)	Worm Expression, CRISPR	Seydoux	Scalable and versatile genome editing using linear DNAs with microhomology to Cas9 Sites in Caenorhabditis elegans. Genetics. 2014 Dec;198(4):1347-56. doi: 10.1534/genetics.114.170423. Epub 2014 Sep 23.
	AP56-8	sgRNA for APa13 (Caenorhabditis elegans)	Worm Expression, CRISPR	Seydoux	Scalable and versatile genome editing using linear DNAs with microhomology to Cas9 Sites in Caenorhabditis elegans. Genetics. 2014 Dec;198(4):1347-56. doi: 10.1534/genetics.114.170423. Epub 2014 Sep 23.
	AP54-3	sgRNA for APs12 (Caenorhabditis elegans)	Worm Expression, CRISPR	Seydoux	Scalable and versatile genome editing using linear DNAs with microhomology to Cas9 Sites in Caenorhabditis elegans. Genetics. 2014 Dec;198(4):1347-56. doi: 10.1534/genetics.114.170423. Epub 2014 Sep 23.
	AP54-5	sgRNA for APs12 (Caenorhabditis elegans)	Worm Expression, CRISPR	Seydoux	Scalable and versatile genome editing using linear DNAs with microhomology to Cas9 Sites in Caenorhabditis elegans. Genetics. 2014 Dec;198(4):1347-56. doi: 10.1534/genetics.114.170423. Epub 2014 Sep 23.
	pIK208	U6 promoter::dpy-10 sgRNA with a "flipped and extended" sgRNA backbone (Caenorhabditis elegans)	Worm Expression, CRISPR	Katic	CRISPR/Cas9 Genome Editing in Caenorhabditis elegans: Evaluation of Templates for Homology-Mediated Repair and Knock-Ins by Homology-Independent DNA Repair. G3 (Bethesda). 2015 Jun 3. pii: g3.115.019273. doi: 10.1534/g3.115.019273.
	pCASCADE-gltA2	gltA2 guide (Synthetic)	Bacterial Expression	Lynch	SUPERSEDE (unpublished)
	pCASCADE-udhA	udhA guide (Synthetic)	Bacterial Expression	Lynch	SUPERSEDE (unpublished)
	pCASCADE-gltA2-udhA	gltA2-udhA guide array (Synthetic)	Bacterial Expression	Lynch	SUPERSEDE (unpublished)
	pCASCADE	control guideRNA (Synthetic)	Bacterial Expression	Lynch	SUPERSEDE (unpublished)
	pCASCADE-zwf	zwf guide (Synthetic)	Bacterial Expression	Lynch	SUPERSEDE (unpublished)
	APq5271	sgRNA for APa4-2	Worm Expression, CRISPR	Seydoux	Scalable and versatile genome editing using linear DNAs with microhomology to Cas9 Sites in Caenorhabditis elegans. Genetics. 2014 Dec;198(4):1347-56. doi: 10.1534/genetics.114.170423. Epub 2014 Sep 23.
	APq5210	sgRNA for APa4-2	Worm Expression, CRISPR	Seydoux	Scalable and versatile genome editing using linear DNAs with microhomology to Cas9 Sites in Caenorhabditis elegans. Genetics. 2014 Dec;198(4):1347-56. doi: 10.1534/genetics.114.170423. Epub 2014 Sep 23.
	AP303-3	sgRNA for APs1	Worm Expression, CRISPR	Seydoux	Scalable and versatile genome editing using linear DNAs with microhomology to Cas9 Sites in Caenorhabditis elegans. Genetics. 2014 Dec;198(4):1347-56. doi: 10.1534/genetics.114.170423. Epub 2014 Sep 23.
	AP303-4	sgRNA for APs1	Worm Expression, CRISPR	Seydoux	Scalable and versatile genome editing using linear DNAs with microhomology to Cas9 Sites in Caenorhabditis elegans. Genetics. 2014 Dec;198(4):1347-56. doi: 10.1534/genetics.114.170423. Epub 2014 Sep 23.
	AP332-2	sgRNA for APs4	Worm Expression, CRISPR	Seydoux	Scalable and versatile genome editing using linear DNAs with microhomology to Cas9 Sites in Caenorhabditis elegans. Genetics. 2014 Dec;198(4):1347-56. doi: 10.1534/genetics.114.170423. Epub 2014 Sep 23.
	AP332-3	sgRNA for APs4	Worm Expression, CRISPR	Seydoux	Scalable and versatile genome editing using linear DNAs with microhomology to Cas9 Sites in Caenorhabditis elegans. Genetics. 2014 Dec;198(4):1347-56. doi: 10.1534/genetics.114.170423. Epub 2014 Sep 23.
	AP332-4	sgRNA for APs4	Worm Expression, CRISPR	Seydoux	Scalable and versatile genome editing using linear DNAs with microhomology to Cas9 Sites in Caenorhabditis elegans. Genetics. 2014 Dec;198(4):1347-56. doi: 10.1534/genetics.114.170423. Epub 2014 Sep 23.
	AP324-2	sgRNA for APs6	Worm Expression, CRISPR	Seydoux	Scalable and versatile genome editing using linear DNAs with microhomology to Cas9 Sites in Caenorhabditis elegans. Genetics. 2014 Dec;198(4):1347-56. doi: 10.1534/genetics.114.170423. Epub 2014 Sep 23.
	AP324-3	sgRNA for APs6	Worm Expression, CRISPR	Seydoux	Scalable and versatile genome editing using linear DNAs with microhomology to Cas9 Sites in Caenorhabditis elegans. Genetics. 2014 Dec;198(4):1347-56. doi: 10.1534/genetics.114.170423. Epub 2014 Sep 23.
	AP324-4	sgRNA for APs6	Worm Expression, CRISPR	Seydoux	Scalable and versatile genome editing using linear DNAs with microhomology to Cas9 Sites in Caenorhabditis elegans. Genetics. 2014 Dec;198(4):1347-56. doi: 10.1534/genetics.114.170423. Epub 2014 Sep 23.
	AP334-2	sgRNA for APs5	Worm Expression, CRISPR	Seydoux	Scalable and versatile genome editing using linear DNAs with microhomology to Cas9 Sites in Caenorhabditis elegans. Genetics. 2014 Dec;198(4):1347-56. doi: 10.1534/genetics.114.170423. Epub 2014 Sep 23.
	AP334-5	sgRNA for APs5	Worm Expression, CRISPR	Seydoux	Scalable and versatile genome editing using linear DNAs with microhomology to Cas9 Sites in Caenorhabditis elegans. Genetics. 2014 Dec;198(4):1347-56. doi: 10.1534/genetics.114.170423. Epub 2014 Sep 23.
	AP334-6	sgRNA for APs5	Worm Expression, CRISPR	Seydoux	Scalable and versatile genome editing using linear DNAs with microhomology to Cas9 Sites in Caenorhabditis elegans. Genetics. 2014 Dec;198(4):1347-56. doi: 10.1534/genetics.114.170423. Epub 2014 Sep 23.
	APCSD 53	sgRNA for CSD 53	Worm Expression, CRISPR	Seydoux	Scalable and versatile genome editing using linear DNAs with microhomology to Cas9 Sites in Caenorhabditis elegans. Genetics. 2014 Dec;198(4):1347-56. doi: 10.1534/genetics.114.170423. Epub 2014 Sep 23.
	APCSD 54	sgRNA for CSD 54	Worm Expression, CRISPR	Seydoux	Scalable and versatile genome editing using linear DNAs with microhomology to Cas9 Sites in Caenorhabditis elegans. Genetics. 2014 Dec;198(4):1347-56. doi: 10.1534/genetics.114.170423. Epub 2014 Sep 23.
	pJZC42	sgRNA + 1XPP7, PCP-VP64 IRES mCherry	Mammalian Expression	Lim	Engineering Complex Synthetic Transcriptional Programs with CRISPR RNA Scaffolds. Cell. 2014 Dec 18. pii: S0092-8674(14)01570-0. doi: 10.1016/j.cell.2014.11.052.
	pJZC43	sgRNA + 2XPP7, PCP-VP64 IRES mCherry	Mammalian Expression	Lim	Engineering Complex Synthetic Transcriptional Programs with CRISPR RNA Scaffolds. Cell. 2014 Dec 18. pii: S0092-8674(14)01570-0. doi: 10.1016/j.cell.2014.11.052.
	pX330.iGFP1	iGFP	Mammalian Expression	Xue	A versatile reporter system for CRISPR-mediated chromosomal rearrangements. Genome Biol. 2015 May 28;16(1):111.
	pX330.iGFP2	iGFP	Mammalian Expression	Xue	A versatile reporter system for CRISPR-mediated chromosomal rearrangements. Genome Biol. 2015 May 28;16(1):111.
	pX330.iGFP3	iGFP	Mammalian Expression	Xue	A versatile reporter system for CRISPR-mediated chromosomal rearrangements. Genome Biol. 2015 May 28;16(1):111.
	pX330.iGFP5	iGFP	Mammalian Expression	Xue	A versatile reporter system for CRISPR-mediated chromosomal rearrangements. Genome Biol. 2015 May 28;16(1):111.
	pX330.LoxPO	LSL-Tomato	Mammalian Expression	Xue	A versatile reporter system for CRISPR-mediated chromosomal rearrangements. Genome Biol. 2015 May 28;16(1):111.
	pX330.LoxP	LoxP sgRNA	Mammalian Expression	Xue	A versatile reporter system for CRISPR-mediated chromosomal rearrangements. Genome Biol. 2015 May 28;16(1):111.
	pX330.Pten.a	Pten deletion (Mus musculus)	Mammalian Expression	Xue	A versatile reporter system for CRISPR-mediated chromosomal rearrangements. Genome Biol. 2015 May 28;16(1):111.
	pX330.Pten.b	Pten deletion (Mus musculus)	Mammalian Expression	Xue	A versatile reporter system for CRISPR-mediated chromosomal rearrangements. Genome Biol. 2015 May 28;16(1):111.
	Lenti-sgLkb1/Cre	sgLkb1 (Mus musculus)	Mammalian Expression, Mouse Targeting, Lentiviral, Cre/Lox, CRISPR	Winslow	Pancreatic cancer modeling using retrograde viral vector delivery and in vivo CRISPR/Cas9-mediated somatic genome editing. Genes Dev. 2015 Jul 15;29(14):1576-85. doi: 10.1101/gad.264861.115. Epub 2015 Jul 15.
	Lenti-sgNT/Cre	sgNT (Mus musculus)	Mammalian Expression, Mouse Targeting, Lentiviral, Cre/Lox, CRISPR	Winslow	Pancreatic cancer modeling using retrograde viral vector delivery and in vivo CRISPR/Cas9-mediated somatic genome editing. Genes Dev. 2015 Jul 15;29(14):1576-85. doi: 10.1101/gad.264861.115. Epub 2015 Jul 15.
	pCAS9-mCherry-TUBB	gRNA TUBB		Hornung	CRISPaint allows modular base-specific gene tagging using a ligase-4-dependent mechanism. Nat Commun. 2016 Jul 28;7:12338. doi: 10.1038/ncomms12338.
	pCAS9-mCherry-ACTG1	gRNA ACTG1 (Homo sapiens)		Hornung	CRISPaint allows modular base-specific gene tagging using a ligase-4-dependent mechanism. Nat Commun. 2016 Jul 28;7:12338. doi: 10.1038/ncomms12338.
	pCAS9-mCherry-HIST1H4C	gRNA HIST1H4C (Homo sapiens)		Hornung	CRISPaint allows modular base-specific gene tagging using a ligase-4-dependent mechanism. Nat Commun. 2016 Jul 28;7:12338. doi: 10.1038/ncomms12338.
	Oct4-Cr1	gRNA for Crispr targeting the OCT4 stop codon (Homo sapiens)	Mammalian Expression	Huangfu	A CRISPR/Cas-Mediated Selection-free Knockin Strategy in Human Embryonic Stem Cells. Stem Cell Reports. 2015 May 28. pii: S2213-6711(15)00128-9. doi: 10.1016/j.stemcr.2015.04.016.
	Lenti-sgNeo/Cre	sgNeo (Mus musculus)	Mammalian Expression, Mouse Targeting, Lentiviral, Cre/Lox, CRISPR	Winslow	Pancreatic cancer modeling using retrograde viral vector delivery and in vivo CRISPR/Cas9-mediated somatic genome editing. Genes Dev. 2015 Jul 15;29(14):1576-85. doi: 10.1101/gad.264861.115. Epub 2015 Jul 15.
	pKLV2-U6gRNA5(gBFP)-PGKGFP2ABFP-W	U6gRNA cassette, PGKGFP2ABFP cassette, WPRE (Homo sapiens), Guide RNA targeting modified BFP	Lentiviral, CRISPR	Yusa	A CRISPR Dropout Screen Identifies Genetic Vulnerabilities and Therapeutic Targets in Acute Myeloid Leukemia. Cell Rep. 2016 Oct 18;17(4):1193-1205. doi: 10.1016/j.celrep.2016.09.079.
	pKLV2-U6gRNA5(gBFP)-PGKmCherry2ABFP-W	U6gRNA cassette, PGKmCherryABFP cassette, WPRE (Homo sapiens), Guide RNA targeting modified BFP (Synthetic)	Lentiviral, CRISPR	Yusa	A CRISPR Dropout Screen Identifies Genetic Vulnerabilities and Therapeutic Targets in Acute Myeloid Leukemia. Cell Rep. 2016 Oct 18;17(4):1193-1205. doi: 10.1016/j.celrep.2016.09.079.
	p426-SNR52p-gRNA.csr-1.Y-SUP4t	gRNA for csr-1 locus (Other)	Yeast Expression, CRISPR ; N. crassa, Fungi	Hong	Efficient gene editing in Neurospora crassa with CRISPR technology Fungal Biology and Biotechnology 2015, 2:4
	pICSL11057	Promote:TaU6+sgRNA HvPM19_1 (Other)	Plant Expression, Synthetic Biology	Patron	Induction of targeted, heritable mutations in barley and Brassica oleracea using RNA-guided Cas9 nuclease. Genome Biol. 2015 Nov 30;16(1):258. doi: 10.1186/s13059-015-0826-7.
	pICSL11058	Promoter_TaU6 + sgRNA_HvPM19_3 (Other)	Plant Expression, Synthetic Biology	Patron	Induction of targeted, heritable mutations in barley and Brassica oleracea using RNA-guided Cas9 nuclease. Genome Biol. 2015 Nov 30;16(1):258. doi: 10.1186/s13059-015-0826-7.
	pICSL11061	Promoter_AtU6-26 + sgRNA_BolC.GA4a-1 (Arabidopsis thaliana)	Plant Expression, Synthetic Biology	Patron	Induction of targeted, heritable mutations in barley and Brassica oleracea using RNA-guided Cas9 nuclease. Genome Biol. 2015 Nov 30;16(1):258. doi: 10.1186/s13059-015-0826-7.
	pICSL11062	Promoter_AtU6-26 + sgRNA_BolC.GA4a-2 (Arabidopsis thaliana)	Plant Expression, Synthetic Biology	Patron	Induction of targeted, heritable mutations in barley and Brassica oleracea using RNA-guided Cas9 nuclease. Genome Biol. 2015 Nov 30;16(1):258. doi: 10.1186/s13059-015-0826-7.
	AAV_3xgRNA;PTENA, p53B,SMAD4A_CAG_FlPO_synthPA	3xgRNA;PTENA, p53B,SMAD4A (Synthetic), FlPO (Synthetic)	Mammalian Expression, AAV ; Flp/Frt	Elverløv-Jakobsen	Cas9/CRISPR pig transposon plasmid_LIR_TRE_CAG_mKateII-Puro-STOP_mVenus-Cas9-PA-RIR (unpublished)
	px330_PTEN exon 5 gRNA	PTEN exon 5 gRNA (Synthetic)	Mammalian Expression	Elverløv-Jakobsen	Cas9/CRISPR pig transposon plasmid_LIR_TRE_CAG_mKateII-Puro-STOP_mVenus-Cas9-PA-RIR (unpublished)
	px330_P53 exon 8 gRNA	P53 exon 8 gRNA (Synthetic)	Mammalian Expression	Elverløv-Jakobsen	Cas9/CRISPR pig transposon plasmid_LIR_TRE_CAG_mKateII-Puro-STOP_mVenus-Cas9-PA-RIR (unpublished)
	px330_SMAD exon 2 gRNA	SMAD exon 2 gRNA (Synthetic)	Mammalian Expression	Elverløv-Jakobsen	Cas9/CRISPR pig transposon plasmid_LIR_TRE_CAG_mKateII-Puro-STOP_mVenus-Cas9-PA-RIR (unpublished)
	px330_P53 exon 7 gRNA	P53 exon 7 gRNA (Synthetic)	Mammalian Expression	Elverløv-Jakobsen	Cas9/CRISPR pig transposon plasmid_LIR_TRE_CAG_mKateII-Puro-STOP_mVenus-Cas9-PA-RIR (unpublished)
	px330_SMAD exon 9 gRNA	SMAD exon 9 gRNA (Synthetic)	Mammalian Expression	Elverløv-Jakobsen	Cas9/CRISPR pig transposon plasmid_LIR_TRE_CAG_mKateII-Puro-STOP_mVenus-Cas9-PA-RIR (unpublished)
	4xgRNA: PTEN exon 5, p53 exon 8, SMAD4 exon 2, p53 exon 7 probasin_mTQ2_FlpO	4xgRNA: PTEN exon 5, p53 exon 8, SMAD4 exon 2, p53 exon 7 (Synthetic), FlpO recombinase (Synthetic)	Mammalian Expression, CRISPR	Elverløv-Jakobsen	Cas9/CRISPR pig transposon plasmid_LIR_TRE_CAG_mKateII-Puro-STOP_mVenus-Cas9-PA-RIR (unpublished)
	5xgRNA: PTEN exon 5, p53 exon 8, SMAD4 exon 2, p53 exon 7, SMAD4 exon 9 probasin_mTQ2_FlpO	5xgRNA: PTEN exon 5, p53 exon 8, SMAD4 exon 2, p53 exon 7, SMAD4 exon 9 (Synthetic), FlpO recombinase (Synthetic)	Mammalian Expression, CRISPR	Elverløv-Jakobsen	Cas9/CRISPR pig transposon plasmid_LIR_TRE_CAG_mKateII-Puro-STOP_mVenus-Cas9-PA-RIR (unpublished)
	3xgRNA: PTEN exon 5, p53 exon 8, SMAD4 exon 2 probasin_mTQ2_FlpO	3xgRNA: PTEN exon 5, p53 exon 8, SMAD4 exon 2 (Synthetic), FlpO (Synthetic)	Mammalian Expression, CRISPR	Elverløv-Jakobsen	Cas9/CRISPR pig transposon plasmid_LIR_TRE_CAG_mKateII-Puro-STOP_mVenus-Cas9-PA-RIR (unpublished)
	pU6_(GLuc)_sgRNA	Gluc sgRNA (Synthetic)	Mammalian Expression, CRISPR	Rinn	Multiplexable, locus-specific targeting of long RNAs with CRISPR-Display. Nat Methods. 2015 Jul;12(7):664-70. doi: 10.1038/nmeth.3433. Epub 2015 Jun 1.
	pCMV/3' Box_(GLuc)_INT	INT construct_bearing three PP7 Stem-loops (Synthetic)	Mammalian Expression, CRISPR, Synthetic Biology	Rinn	Multiplexable, locus-specific targeting of long RNAs with CRISPR-Display. Nat Methods. 2015 Jul;12(7):664-70. doi: 10.1038/nmeth.3433. Epub 2015 Jun 1.
	PAX6 sgRNA2	sgRNA targeting PAX6 (Homo sapiens)	Mammalian Expression, CRISPR	Zhang	Engineering Human Stem Cell Lines with Inducible Gene Knockout using CRISPR/Cas9. Cell Stem Cell. 2015 Jul 1. pii: S1934-5909(15)00261-1. doi: 10.1016/j.stem.2015.06.001.
	PAX6 sgRNA7	sgRNA targeting PAX6 (Homo sapiens)	Mammalian Expression, CRISPR	Zhang	Engineering Human Stem Cell Lines with Inducible Gene Knockout using CRISPR/Cas9. Cell Stem Cell. 2015 Jul 1. pii: S1934-5909(15)00261-1. doi: 10.1016/j.stem.2015.06.001.
	pX330-sgRNA_Dicer_1	sgRNA mouse Dicer1 (Mus musculus)	Mammalian Expression, Bacterial Expression, Mouse Targeting, CRISPR	Ciaudo	Dicer, a new regulator of pluripotency exit and LINE-1 elements in mouse embryonic stem cells. FEBS Open Bio. 2017 Jan 11;7(2):204-220. doi: 10.1002/2211-5463.12174. eCollection 2017 Feb.
	pX330-sgRNA_Dicer_2	sgRNA mouse Dicer1 (Mus musculus)	Mammalian Expression, Bacterial Expression, Mouse Targeting, CRISPR	Ciaudo	Dicer, a new regulator of pluripotency exit and LINE-1 elements in mouse embryonic stem cells. FEBS Open Bio. 2017 Jan 11;7(2):204-220. doi: 10.1002/2211-5463.12174. eCollection 2017 Feb.
	pX330-sgRNA_Dicer_3	sgRNA mouse Dicer1 (Mus musculus)	Mammalian Expression, Bacterial Expression, Mouse Targeting, CRISPR	Ciaudo	Dicer, a new regulator of pluripotency exit and LINE-1 elements in mouse embryonic stem cells. FEBS Open Bio. 2017 Jan 11;7(2):204-220. doi: 10.1002/2211-5463.12174. eCollection 2017 Feb.
	pMM704	dCas9, LacI, sgRNA		Lu	Programming a Human Commensal Bacterium, Bacteroides thetaiotaomicron, to Sense and Respond to Stimuli in the Murine Gut Microbiota. Cell Syst. 2015 Jul 29;1(1):62-71. doi: 10.1016/j.cels.2015.06.001.
	pMM705	dCas9, LacI, sgRNA		Lu	Programming a Human Commensal Bacterium, Bacteroides thetaiotaomicron, to Sense and Respond to Stimuli in the Murine Gut Microbiota. Cell Syst. 2015 Jul 29;1(1):62-71. doi: 10.1016/j.cels.2015.06.001.
	pMM710	dCas9, LacI, sgRNA		Lu	Programming a Human Commensal Bacterium, Bacteroides thetaiotaomicron, to Sense and Respond to Stimuli in the Murine Gut Microbiota. Cell Syst. 2015 Jul 29;1(1):62-71. doi: 10.1016/j.cels.2015.06.001.
	pMM725	dCas9, LacI, sgRNA, NanoLuc		Lu	Programming a Human Commensal Bacterium, Bacteroides thetaiotaomicron, to Sense and Respond to Stimuli in the Murine Gut Microbiota. Cell Syst. 2015 Jul 29;1(1):62-71. doi: 10.1016/j.cels.2015.06.001.
	pMM731	dCas9, LacI, sgRNA, NanoLuc		Lu	Programming a Human Commensal Bacterium, Bacteroides thetaiotaomicron, to Sense and Respond to Stimuli in the Murine Gut Microbiota. Cell Syst. 2015 Jul 29;1(1):62-71. doi: 10.1016/j.cels.2015.06.001.
	pMM732	dCas9, LacI, sgRNA, NanoLuc		Lu	Programming a Human Commensal Bacterium, Bacteroides thetaiotaomicron, to Sense and Respond to Stimuli in the Murine Gut Microbiota. Cell Syst. 2015 Jul 29;1(1):62-71. doi: 10.1016/j.cels.2015.06.001.
	pMM733	dCas9, LacI, sgRNA, NanoLuc		Lu	Programming a Human Commensal Bacterium, Bacteroides thetaiotaomicron, to Sense and Respond to Stimuli in the Murine Gut Microbiota. Cell Syst. 2015 Jul 29;1(1):62-71. doi: 10.1016/j.cels.2015.06.001.
	pMM750	dCas9, LacI, sgRNA, NanoLuc		Lu	Programming a Human Commensal Bacterium, Bacteroides thetaiotaomicron, to Sense and Respond to Stimuli in the Murine Gut Microbiota. Cell Syst. 2015 Jul 29;1(1):62-71. doi: 10.1016/j.cels.2015.06.001.
	pMM763	dCas9, LacI, sgRNA, NanoLuc		Lu	Programming a Human Commensal Bacterium, Bacteroides thetaiotaomicron, to Sense and Respond to Stimuli in the Murine Gut Microbiota. Cell Syst. 2015 Jul 29;1(1):62-71. doi: 10.1016/j.cels.2015.06.001.
	pMM764	dCas9, LacI, sgRNA, NanoLuc		Lu	Programming a Human Commensal Bacterium, Bacteroides thetaiotaomicron, to Sense and Respond to Stimuli in the Murine Gut Microbiota. Cell Syst. 2015 Jul 29;1(1):62-71. doi: 10.1016/j.cels.2015.06.001.
	pLKO1-puro-U6-sgRNA-TS2	TS2 sgRNA (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Wolfe	DNA-binding-domain fusions enhance the targeting range and precision of Cas9. Nat Methods. 2015 Oct 19. doi: 10.1038/nmeth.3624.
	pLKO1-puro-U6-sgRNA-TS3	TS3 sgRNA (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Wolfe	DNA-binding-domain fusions enhance the targeting range and precision of Cas9. Nat Methods. 2015 Oct 19. doi: 10.1038/nmeth.3624.
	pLKO1-puro-U6-sgRNA-TS4	TS4 sgRNA (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Wolfe	DNA-binding-domain fusions enhance the targeting range and precision of Cas9. Nat Methods. 2015 Oct 19. doi: 10.1038/nmeth.3624.
	pLKO1-puro-U6-sgRNA-LRTM2	LRTM2 sgRNA (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Wolfe	DNA-binding-domain fusions enhance the targeting range and precision of Cas9. Nat Methods. 2015 Oct 19. doi: 10.1038/nmeth.3624.
	pLKO1-puro-U6-sgRNA-KANK3	KANK3 sgRNA (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Wolfe	DNA-binding-domain fusions enhance the targeting range and precision of Cas9. Nat Methods. 2015 Oct 19. doi: 10.1038/nmeth.3624.
	pLKO1-puro-U6-sgRNA-TGM2	TGM2 sgRNA (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Wolfe	DNA-binding-domain fusions enhance the targeting range and precision of Cas9. Nat Methods. 2015 Oct 19. doi: 10.1038/nmeth.3624.
	pLKO1-puro-U6-sgRNA-PLXNB2	PLXNB2 sgRNA (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Wolfe	DNA-binding-domain fusions enhance the targeting range and precision of Cas9. Nat Methods. 2015 Oct 19. doi: 10.1038/nmeth.3624.
	GG-EBNA-OCT4	5 concatenated gRNA transcriptional cassettes targeting OCT4	CRISPR	Otonkoski	Conditionally Stabilized dCas9 Activator for Controlling Gene Expression in Human Cell Reprogramming and Differentiation. Stem Cell Reports. 2015 Sep 8;5(3):448-59. doi: 10.1016/j.stemcr.2015.08.001.
	HPRT2b-pGEM	HPRT gRNA2 (Homo sapiens)	Mammalian Expression	Yan	Enriching CRISPR-Cas9 targeted cells by co-targeting the HPRT gene. Nucleic Acids Res. 2015 Jun 29. pii: gkv675.
	pZDonor-U6-myogenin stop codon gRNA	Myogenin C terminus-targeting guide RNA (Mus musculus)	CRISPR	Gersbach	TMG reporters (unpublished)
	pX330-mitf	mitf-sgRNA (Other), SpCas9 (Synthetic)	Mammalian Expression	Zhao	Efficient CRISPR/Cas9-mediated biallelic gene disruption and site-specific knockin after rapid selection of highly active sgRNAs in pigs. Sci Rep. 2015 Aug 21;5:13348. doi: 10.1038/srep13348.
	CRISPRmTmG2	gRNA that targets near LoxP sites		Cepko	A gene regulatory network controls the binary fate decision of rod and bipolar cells in the vertebrate retina. Dev Cell. 2014 Sep 8;30(5):513-27. doi: 10.1016/j.devcel.2014.07.018. Epub 2014 Aug 21.
	pTC217	Nuclease (Cas9/sgRNA) + Donor + GVR (Other)	Plant Expression, CRISPR	Voytas	High-frequency, precise modification of the tomato genome. Genome Biol. 2015 Nov 6;16(1):232. doi: 10.1186/s13059-015-0796-9.
	pTC223	Nuclease (Cas9/sgRNA) + Donor + GVR (Other)	Plant Expression, CRISPR	Voytas	High-frequency, precise modification of the tomato genome. Genome Biol. 2015 Nov 6;16(1):232. doi: 10.1186/s13059-015-0796-9.
	AP568-2	sgRNA for dpy‐10	Worm Expression, CRISPR	Seydoux	High Efficiency, Homology-Directed Genome Editing in Caenorhabditis elegans Using CRISPR-Cas9 Ribonucleoprotein Complexes. Genetics. 2015 Sep;201(1):47-54. doi: 10.1534/genetics.115.179382. Epub 2015 Jul 17.
	AP568-3	sgRNA for dpy‐10	Worm Expression, CRISPR	Seydoux	High Efficiency, Homology-Directed Genome Editing in Caenorhabditis elegans Using CRISPR-Cas9 Ribonucleoprotein Complexes. Genetics. 2015 Sep;201(1):47-54. doi: 10.1534/genetics.115.179382. Epub 2015 Jul 17.
	lentiCRISPR - C16orf80 sgRNA 2	sgRNA against C16orf80	Mammalian Expression, Lentiviral, CRISPR	Sabatini	Identification and characterization of essential genes in the human genome. Science. 2015 Oct 15. pii: aac7041.
	lentiCRISPR - C16orf80 sgRNA 1	sgRNA against C16orf80	Mammalian Expression, Lentiviral, CRISPR	Sabatini	Identification and characterization of essential genes in the human genome. Science. 2015 Oct 15. pii: aac7041.
	lentiCRISPR - C3orf17 sgRNA 1	sgRNA against C3orf17	Mammalian Expression, Lentiviral, CRISPR	Sabatini	Identification and characterization of essential genes in the human genome. Science. 2015 Oct 15. pii: aac7041.
	lentiCRISPR - C3orf17 sgRNA 2	sgRNA against C3orf17	Mammalian Expression, Lentiviral, CRISPR	Sabatini	Identification and characterization of essential genes in the human genome. Science. 2015 Oct 15. pii: aac7041.
	lentiCRISPR - C9orf114 sgRNA 2	sgRNA against C9orf114	Mammalian Expression, Lentiviral, CRISPR	Sabatini	Identification and characterization of essential genes in the human genome. Science. 2015 Oct 15. pii: aac7041.
	lentiCRISPR - C9orf114 sgRNA 1	sgRNA against C9orf114	Mammalian Expression, Lentiviral, CRISPR	Sabatini	Identification and characterization of essential genes in the human genome. Science. 2015 Oct 15. pii: aac7041.
	lentiCRISPR - DDX3Y sgRNA 1	sgRNA against DDX3Y	Mammalian Expression, Lentiviral, CRISPR	Sabatini	Identification and characterization of essential genes in the human genome. Science. 2015 Oct 15. pii: aac7041.
	lentiCRISPR - DDX3Y sgRNA 2	sgRNA against DDX3Y	Mammalian Expression, Lentiviral, CRISPR	Sabatini	Identification and characterization of essential genes in the human genome. Science. 2015 Oct 15. pii: aac7041.
	lentiCRISPR - Amplicon, BCR/ABL sgRNA 2	sgRNA against BCR/ABLamplicon found in CML-derived K562 cell line	Mammalian Expression, Lentiviral, CRISPR	Sabatini	Identification and characterization of essential genes in the human genome. Science. 2015 Oct 15. pii: aac7041.
	lentiCRISPR - Amplicon, BCR/ABL sgRNA 1	sgRNA against BCR/ABLamplicon found in CML-derived K562 cell line	Mammalian Expression, Lentiviral, CRISPR	Sabatini	Identification and characterization of essential genes in the human genome. Science. 2015 Oct 15. pii: aac7041.
	lentiCRISPR - Amplicon, JAK2 sgRNA 2	sgRNA against JAK2 amplicon found in AML-derived HEL cell line	Mammalian Expression, Lentiviral, CRISPR	Sabatini	Identification and characterization of essential genes in the human genome. Science. 2015 Oct 15. pii: aac7041.
	lentiCRISPR - AAVS1 sgRNA	sgRNA against AAVS1	Mammalian Expression, Lentiviral, CRISPR	Sabatini	Identification and characterization of essential genes in the human genome. Science. 2015 Oct 15. pii: aac7041.
	lentiCRISPR - CTRL sgRNA	non-targeting sgRNA	Mammalian Expression, Lentiviral, CRISPR	Sabatini	Identification and characterization of essential genes in the human genome. Science. 2015 Oct 15. pii: aac7041.
	lentiCRISPR - Amplicon, JAK2 sgRNA 1	sgRNA against JAK2 amplicon found in AML-derived HEL cell line	Mammalian Expression, Lentiviral, CRISPR	Sabatini	Identification and characterization of essential genes in the human genome. Science. 2015 Oct 15. pii: aac7041.
	pHEE2E-TRI	sgRNA targeting TRY and CPC genes (Synthetic), sgRNA targeting ETC2 gene (Synthetic), zCas9 (Other)	Plant Expression, CRISPR ; Plant binary vector	Chen	Egg cell-specific promoter-controlled CRISPR/Cas9 efficiently generates homozygous mutants for multiple target genes in Arabidopsis in a single generation. Genome Biol. 2015 Jul 21;16:144. doi: 10.1186/s13059-015-0715-0.
	pRB1081	dpy-10 gRNA (Synthetic)	Worm Expression, CRISPR	Fire	Cas9 Variants Expand the Target Repertoire in Caenorhabditis elegans. Genetics. 2015 Dec 17. pii: genetics.115.185041.
	sgPal1 (p2Tol-U6-sgPal1-HygR)	sgPal1		Sherwood	Cloning-free CRISPR. Stem Cell Reports. 2015 Oct 27. pii: S2213-6711(15)00284-2. doi: 10.1016/j.stemcr.2015.09.022.
	sgPal7 (p2Tol-U6-sgPal7-HygR)	sgPal7		Sherwood	Cloning-free CRISPR. Stem Cell Reports. 2015 Oct 27. pii: S2213-6711(15)00284-2. doi: 10.1016/j.stemcr.2015.09.022.
	pRB1084	dpy-10 gRNA (Synthetic)	Worm Expression, CRISPR	Fire	Cas9 Variants Expand the Target Repertoire in Caenorhabditis elegans. Genetics. 2015 Dec 17. pii: genetics.115.185041.
	SS9_RNA	SS9 gRNA (Other)	Bacterial Expression, CRISPR	Gill	Rapid and Efficient One-Step Metabolic Pathway Integration in E. coli. ACS Synth Biol. 2016 Apr 22.
	pdCas9-DNMT3A-PuroR_BACH2-sgRNA8	BACH2-sgRNA8 (Homo sapiens)	Mammalian Expression, CRISPR	Zoldoš	Repurposing the CRISPR-Cas9 system for targeted DNA methylation. Nucleic Acids Res. 2016 Mar 11. pii: gkw159.
	pdCas9-DNMT3A-PuroR_BACH2-sgRNA8 (ANV)	BACH2-sgRNA8 (Homo sapiens)	Mammalian Expression, CRISPR	Zoldoš	Repurposing the CRISPR-Cas9 system for targeted DNA methylation. Nucleic Acids Res. 2016 Mar 11. pii: gkw159.
	pdCas9-DNMT3A-PuroR_hNT	Non-targeting sgRNA human (Homo sapiens)	Mammalian Expression, CRISPR	Zoldoš	Repurposing the CRISPR-Cas9 system for targeted DNA methylation. Nucleic Acids Res. 2016 Mar 11. pii: gkw159.
	PX458_ATF4_1	ATF4 (Homo sapiens)	CRISPR	Mendenhall	Mendenhall CRISPR tagging plasmids (unpublished)
	PX458_ATF4_2	ATF4 (Homo sapiens)	CRISPR	Mendenhall	Mendenhall CRISPR tagging plasmids (unpublished)
	PX458_HHEX_1	HHEX (Homo sapiens)	CRISPR	Mendenhall	Mendenhall CRISPR tagging plasmids (unpublished)
	PX458_HHEX_2	HHEX (Homo sapiens)	CRISPR	Mendenhall	Mendenhall CRISPR tagging plasmids (unpublished)
	PX458_PBX2_1	PBX2 (Homo sapiens)	CRISPR	Mendenhall	Mendenhall CRISPR tagging plasmids (unpublished)
	PX458_PBX2_2	PBX2 (Homo sapiens)	CRISPR	Mendenhall	Mendenhall CRISPR tagging plasmids (unpublished)
	PX458_ZNF146_1	ZNF146 (Homo sapiens)	CRISPR	Mendenhall	Mendenhall CRISPR tagging plasmids (unpublished)
	PX458_ZNF146_2	ZNF146 (Homo sapiens)	CRISPR	Mendenhall	Mendenhall CRISPR tagging plasmids (unpublished)
	PX458_ZNF3_1	ZNF3 (Homo sapiens)	CRISPR	Mendenhall	Mendenhall CRISPR tagging plasmids (unpublished)
	PX458_ZNF3_2	ZNF3 (Homo sapiens)	CRISPR	Mendenhall	Mendenhall CRISPR tagging plasmids (unpublished)
	PX458_DNMT3B	DNMT3B (Homo sapiens)	CRISPR	Mendenhall	Mendenhall CRISPR tagging plasmids (unpublished)
	PX458_ZNF219_1	ZNF219 (Homo sapiens)	CRISPR	Mendenhall	Mendenhall CRISPR tagging plasmids (unpublished)
	PX458_ZNF219_2	ZNF219 (Homo sapiens)	CRISPR	Mendenhall	Mendenhall CRISPR tagging plasmids (unpublished)
	PX458_SSRP1_1	SSRP1 (Homo sapiens)	CRISPR	Mendenhall	Mendenhall CRISPR tagging plasmids (unpublished)
	PX458_SSRP1_2	SSRP1 (Homo sapiens)	CRISPR	Mendenhall	Mendenhall CRISPR tagging plasmids (unpublished)
	PX458_TGIF2_1	TGIF2 (Homo sapiens)	CRISPR	Mendenhall	Mendenhall CRISPR tagging plasmids (unpublished)
	PX458_TGIF2_2	TGIF2 (Homo sapiens)	CRISPR	Mendenhall	Mendenhall CRISPR tagging plasmids (unpublished)
	pDECKO_GFP	gRNAs toward GFP (Synthetic)	Mammalian Expression	Guigo	DECKO: Single-oligo, dual-CRISPR deletion of genomic elements including long non-coding RNAs. BMC Genomics. 2015 Oct 23;16(1):846. doi: 10.1186/s12864-015-2086-z.
	pDECKO_Malat1_A	gRNAs toward Malat1 (Homo sapiens)	Mammalian Expression	Guigo	DECKO: Single-oligo, dual-CRISPR deletion of genomic elements including long non-coding RNAs. BMC Genomics. 2015 Oct 23;16(1):846. doi: 10.1186/s12864-015-2086-z.
	pDECKO_Malat1_B	gRNAs toward Malat1 (Homo sapiens)	Mammalian Expression	Guigo	DECKO: Single-oligo, dual-CRISPR deletion of genomic elements including long non-coding RNAs. BMC Genomics. 2015 Oct 23;16(1):846. doi: 10.1186/s12864-015-2086-z.
	pDECKO_Malat1_C	gRNAs toward Malat1 (Homo sapiens)	Mammalian Expression	Guigo	DECKO: Single-oligo, dual-CRISPR deletion of genomic elements including long non-coding RNAs. BMC Genomics. 2015 Oct 23;16(1):846. doi: 10.1186/s12864-015-2086-z.
	pDECKO_Malat1_D	gRNAs toward Malat1 (Homo sapiens)	Mammalian Expression	Guigo	DECKO: Single-oligo, dual-CRISPR deletion of genomic elements including long non-coding RNAs. BMC Genomics. 2015 Oct 23;16(1):846. doi: 10.1186/s12864-015-2086-z.
	pDECKO_Malat1_E	gRNAs toward Malat1 (Homo sapiens)	Mammalian Expression	Guigo	DECKO: Single-oligo, dual-CRISPR deletion of genomic elements including long non-coding RNAs. BMC Genomics. 2015 Oct 23;16(1):846. doi: 10.1186/s12864-015-2086-z.
	pDECKO_TFRC_B	gRNAs toward TFRC (Homo sapiens)	Mammalian Expression	Guigo	DECKO: Single-oligo, dual-CRISPR deletion of genomic elements including long non-coding RNAs. BMC Genomics. 2015 Oct 23;16(1):846. doi: 10.1186/s12864-015-2086-z.
	pDECKO_TFRC_C (with mCherry)	gRNAs toward TFRC (Homo sapiens)	Mammalian Expression	Guigo	DECKO: Single-oligo, dual-CRISPR deletion of genomic elements including long non-coding RNAs. BMC Genomics. 2015 Oct 23;16(1):846. doi: 10.1186/s12864-015-2086-z.
	pDECKO_UCA1	gRNAs toward UCA1 (Homo sapiens)	Mammalian Expression	Guigo	DECKO: Single-oligo, dual-CRISPR deletion of genomic elements including long non-coding RNAs. BMC Genomics. 2015 Oct 23;16(1):846. doi: 10.1186/s12864-015-2086-z.
	AAVS1 T2 CRIPR in pX330	Cas9 and gRNA for targeting the AAVS1 locus in human cells (Homo sapiens)	Mammalian Expression, CRISPR	Kanemaki	Rapid Protein Depletion in Human Cells by Auxin-Inducible Degron Tagging with Short Homology Donors. Cell Rep. 2016 Apr 5;15(1):210-8. doi: 10.1016/j.celrep.2016.03.001. Epub 2016 Mar 24.
	pT7-gRNA_zebrafish_mmp21	zebrafish mmp21 (Danio rerio)	CRISPR	Katsanis	A human laterality disorder caused by a homozygous deleterious mutation in MMP21. J Med Genet. 2015 Dec;52(12):840-7. doi: 10.1136/jmedgenet-2015-103336. Epub 2015 Oct 1.
	pMD19T-slr0230-PL31-sgRNANT1-KmR	PL31-sgRNA NT1	Bacterial Expression	Hudson	Multiple Gene Repression in Cyanobacteria Using CRISPRi. ACS Synth Biol. 2015 Dec 28.
	pMD19T-slr0230-PL22-sgRNANT1-KmR	PL22-sgRNA NT1	Bacterial Expression	Hudson	Multiple Gene Repression in Cyanobacteria Using CRISPRi. ACS Synth Biol. 2015 Dec 28.
	pNICKclos2.0	Cas9 nickase (Other), sgRNA to xylR (Synthetic)	E.coli - clostridium shuttle vector	Yang	CRISPR-based genome editing and expression control systems in Clostridium acetobutylicum and Clostridium beijerinckii. Biotechnol J. 2016 May 23. doi: 10.1002/biot.201600053.
	pCfB3020(gRNA X-2)	guiding RNA (Saccharomyces cerevisiae)	Yeast Expression, CRISPR ; gRNA	Borodina	EasyClone-MarkerFree: A vector toolkit for marker-less integration of genes into Saccharomyces cerevisiae via CRISPR-Cas9. Biotechnol J. 2016 Aug;11(8):1110-7. doi: 10.1002/biot.201600147. Epub 2016 Jun 23.
	pCfB3041(gRNA X-3)	guiding RNA (Saccharomyces cerevisiae)	Yeast Expression, CRISPR ; gRNA	Borodina	EasyClone-MarkerFree: A vector toolkit for marker-less integration of genes into Saccharomyces cerevisiae via CRISPR-Cas9. Biotechnol J. 2016 Aug;11(8):1110-7. doi: 10.1002/biot.201600147. Epub 2016 Jun 23.
	pCfB3042(gRNA X-4)	guiding RNA (Saccharomyces cerevisiae)	Yeast Expression, CRISPR ; gRNA	Borodina	EasyClone-MarkerFree: A vector toolkit for marker-less integration of genes into Saccharomyces cerevisiae via CRISPR-Cas9. Biotechnol J. 2016 Aug;11(8):1110-7. doi: 10.1002/biot.201600147. Epub 2016 Jun 23.
	pCfB3043(gRNA XI-1)	guiding RNA (Saccharomyces cerevisiae)	Yeast Expression, CRISPR ; gRNA	Borodina	EasyClone-MarkerFree: A vector toolkit for marker-less integration of genes into Saccharomyces cerevisiae via CRISPR-Cas9. Biotechnol J. 2016 Aug;11(8):1110-7. doi: 10.1002/biot.201600147. Epub 2016 Jun 23.
	pCfB3044(gRNA XI-2)	guiding RNA (Saccharomyces cerevisiae)	Yeast Expression, CRISPR ; gRNA	Borodina	EasyClone-MarkerFree: A vector toolkit for marker-less integration of genes into Saccharomyces cerevisiae via CRISPR-Cas9. Biotechnol J. 2016 Aug;11(8):1110-7. doi: 10.1002/biot.201600147. Epub 2016 Jun 23.
	pCfB3045(gRNA XI-3)	guiding RNA (Saccharomyces cerevisiae)	Yeast Expression, CRISPR ; gRNA	Borodina	EasyClone-MarkerFree: A vector toolkit for marker-less integration of genes into Saccharomyces cerevisiae via CRISPR-Cas9. Biotechnol J. 2016 Aug;11(8):1110-7. doi: 10.1002/biot.201600147. Epub 2016 Jun 23.
	pCfB3046(gRNA XI-5)	guiding RNA (Saccharomyces cerevisiae)	Yeast Expression, CRISPR ; gRNA	Borodina	EasyClone-MarkerFree: A vector toolkit for marker-less integration of genes into Saccharomyces cerevisiae via CRISPR-Cas9. Biotechnol J. 2016 Aug;11(8):1110-7. doi: 10.1002/biot.201600147. Epub 2016 Jun 23.
	pCfB3047(gRNA XII-1)	guiding RNA (Saccharomyces cerevisiae)	Yeast Expression, CRISPR ; gRNA	Borodina	EasyClone-MarkerFree: A vector toolkit for marker-less integration of genes into Saccharomyces cerevisiae via CRISPR-Cas9. Biotechnol J. 2016 Aug;11(8):1110-7. doi: 10.1002/biot.201600147. Epub 2016 Jun 23.
	pCfB3048(gRNA XII-2)	guiding RNA (Saccharomyces cerevisiae)	Yeast Expression, CRISPR ; gRNA	Borodina	EasyClone-MarkerFree: A vector toolkit for marker-less integration of genes into Saccharomyces cerevisiae via CRISPR-Cas9. Biotechnol J. 2016 Aug;11(8):1110-7. doi: 10.1002/biot.201600147. Epub 2016 Jun 23.
	pCfB3049(gRNA XII-4)	guiding RNA (Saccharomyces cerevisiae)	Yeast Expression, CRISPR ; gRNA	Borodina	EasyClone-MarkerFree: A vector toolkit for marker-less integration of genes into Saccharomyces cerevisiae via CRISPR-Cas9. Biotechnol J. 2016 Aug;11(8):1110-7. doi: 10.1002/biot.201600147. Epub 2016 Jun 23.
	pCfB3050(gRNA XII-5)	guiding RNA (Saccharomyces cerevisiae)	Yeast Expression, CRISPR ; gRNA	Borodina	EasyClone-MarkerFree: A vector toolkit for marker-less integration of genes into Saccharomyces cerevisiae via CRISPR-Cas9. Biotechnol J. 2016 Aug;11(8):1110-7. doi: 10.1002/biot.201600147. Epub 2016 Jun 23.
	pCfB3051(gRNA X-3 XI-2 XII-2)	guiding RNA (Saccharomyces cerevisiae)	Yeast Expression, CRISPR ; gRNA	Borodina	EasyClone-MarkerFree: A vector toolkit for marker-less integration of genes into Saccharomyces cerevisiae via CRISPR-Cas9. Biotechnol J. 2016 Aug;11(8):1110-7. doi: 10.1002/biot.201600147. Epub 2016 Jun 23.
	pCfB3052(gRNA X-4, XI-3, XII-5)	guiding RNA (Saccharomyces cerevisiae)	Yeast Expression, CRISPR ; gRNA	Borodina	EasyClone-MarkerFree: A vector toolkit for marker-less integration of genes into Saccharomyces cerevisiae via CRISPR-Cas9. Biotechnol J. 2016 Aug;11(8):1110-7. doi: 10.1002/biot.201600147. Epub 2016 Jun 23.
	pCfB3053(gRNA X-2, XI-5, XII-4)	guiding RNA (Saccharomyces cerevisiae)	Yeast Expression, CRISPR ; gRNA	Borodina	EasyClone-MarkerFree: A vector toolkit for marker-less integration of genes into Saccharomyces cerevisiae via CRISPR-Cas9. Biotechnol J. 2016 Aug;11(8):1110-7. doi: 10.1002/biot.201600147. Epub 2016 Jun 23.
	pdCas9-M-C4	Repressor C4 (orthogonal T7-lac repressor) (Synthetic)	Bacterial Expression, CRISPR, Synthetic Biology	Koffas	Rapid generation of CRISPR/dCas9-regulated, orthogonally repressible hybrid T7-lac promoters for modular, tuneable control of metabolic pathway fluxes in Escherichia coli. Nucleic Acids Res. 2016 Apr 13. pii: gkw231.
	pdCas9-M-3F2	Repressor C4 (orthogonal T7-lac repressor) (Synthetic)	Bacterial Expression, CRISPR, Synthetic Biology	Koffas	Rapid generation of CRISPR/dCas9-regulated, orthogonally repressible hybrid T7-lac promoters for modular, tuneable control of metabolic pathway fluxes in Escherichia coli. Nucleic Acids Res. 2016 Apr 13. pii: gkw231.
	pdCas9-M-3H5	Repressor 3H5 (orthogonal T7-lac repressor) (Synthetic)	Bacterial Expression, CRISPR, Synthetic Biology	Koffas	Rapid generation of CRISPR/dCas9-regulated, orthogonally repressible hybrid T7-lac promoters for modular, tuneable control of metabolic pathway fluxes in Escherichia coli. Nucleic Acids Res. 2016 Apr 13. pii: gkw231.
	pdCas9-M-1B6	Repressor 1B6 (orthogonal T7-lac repressor) (Synthetic)	Bacterial Expression, CRISPR, Synthetic Biology	Koffas	Rapid generation of CRISPR/dCas9-regulated, orthogonally repressible hybrid T7-lac promoters for modular, tuneable control of metabolic pathway fluxes in Escherichia coli. Nucleic Acids Res. 2016 Apr 13. pii: gkw231.
	pdCas9-M-4F2	Repressor 4F2 (orthogonal T7-lac repressor) (Synthetic)	Bacterial Expression, CRISPR, Synthetic Biology	Koffas	Rapid generation of CRISPR/dCas9-regulated, orthogonally repressible hybrid T7-lac promoters for modular, tuneable control of metabolic pathway fluxes in Escherichia coli. Nucleic Acids Res. 2016 Apr 13. pii: gkw231.
	pdCas9-M-5F5	Repressor 5F5 (orthogonal T7-lac repressor) (Synthetic)	Bacterial Expression, CRISPR, Synthetic Biology	Koffas	Rapid generation of CRISPR/dCas9-regulated, orthogonally repressible hybrid T7-lac promoters for modular, tuneable control of metabolic pathway fluxes in Escherichia coli. Nucleic Acids Res. 2016 Apr 13. pii: gkw231.
	pdCas9-M-1D4	Repressor 1D4 (orthogonal T7-lac repressor) (Synthetic)	Bacterial Expression, CRISPR, Synthetic Biology	Koffas	Rapid generation of CRISPR/dCas9-regulated, orthogonally repressible hybrid T7-lac promoters for modular, tuneable control of metabolic pathway fluxes in Escherichia coli. Nucleic Acids Res. 2016 Apr 13. pii: gkw231.
	pdCas9-M-4A6	Repressor 4A6 (orthogonal T7-lac repressor) (Synthetic)	Bacterial Expression, CRISPR, Synthetic Biology	Koffas	Rapid generation of CRISPR/dCas9-regulated, orthogonally repressible hybrid T7-lac promoters for modular, tuneable control of metabolic pathway fluxes in Escherichia coli. Nucleic Acids Res. 2016 Apr 13. pii: gkw231.
	pdCas9-M-3A2	Repressor 3A2 (orthogonal T7-lac repressor) (Synthetic)	Bacterial Expression, CRISPR, Synthetic Biology	Koffas	Rapid generation of CRISPR/dCas9-regulated, orthogonally repressible hybrid T7-lac promoters for modular, tuneable control of metabolic pathway fluxes in Escherichia coli. Nucleic Acids Res. 2016 Apr 13. pii: gkw231.
	pdCas9-M-1E4	Repressor 1E4 (orthogonal T7-lac repressor) (Synthetic)	Bacterial Expression, CRISPR, Synthetic Biology	Koffas	Rapid generation of CRISPR/dCas9-regulated, orthogonally repressible hybrid T7-lac promoters for modular, tuneable control of metabolic pathway fluxes in Escherichia coli. Nucleic Acids Res. 2016 Apr 13. pii: gkw231.
	pdCas9-M-G6	Repressor G6 (orthogonal T7-lac repressor) (Synthetic)	Bacterial Expression, CRISPR, Synthetic Biology	Koffas	Rapid generation of CRISPR/dCas9-regulated, orthogonally repressible hybrid T7-lac promoters for modular, tuneable control of metabolic pathway fluxes in Escherichia coli. Nucleic Acids Res. 2016 Apr 13. pii: gkw231.
	pX330-sgRNA_Dgcr8_1	DGCR8 (Mus musculus)	Mammalian Expression, CRISPR	Ciaudo	Noncanonical function of DGCR8 controls mESC exit from pluripotency. J Cell Biol. 2017 Jan 18. pii: jcb.201606073. doi: 10.1083/jcb.201606073.
	pX330-sgRNA_Dgcr8_2	DGCR8 (Mus musculus)	Mammalian Expression, CRISPR	Ciaudo	Noncanonical function of DGCR8 controls mESC exit from pluripotency. J Cell Biol. 2017 Jan 18. pii: jcb.201606073. doi: 10.1083/jcb.201606073.
	pX330-sgRNA_Dgcr8_3	DGCR8 (Mus musculus)	Mammalian Expression, CRISPR	Ciaudo	Noncanonical function of DGCR8 controls mESC exit from pluripotency. J Cell Biol. 2017 Jan 18. pii: jcb.201606073. doi: 10.1083/jcb.201606073.
	pX330-sgRNA_Dgcr8_4	DGCR8 (Mus musculus)	Mammalian Expression, CRISPR	Ciaudo	Noncanonical function of DGCR8 controls mESC exit from pluripotency. J Cell Biol. 2017 Jan 18. pii: jcb.201606073. doi: 10.1083/jcb.201606073.
	pX330-sgRNA_Ago2_1	AGO2 (Mus musculus)	Mammalian Expression, CRISPR	Ciaudo	Argonaute 2 Is Required for Extra-embryonic Endoderm Differentiation of Mouse Embryonic Stem Cells. Stem Cell Reports. 2018 Jan 24. pii: S2213-6711(17)30571-4. doi: 10.1016/j.stemcr.2017.12.023.
	pX330-sgRNA_Ago2_2	AGO2 (Mus musculus)	Mammalian Expression, CRISPR	Ciaudo	Argonaute 2 Is Required for Extra-embryonic Endoderm Differentiation of Mouse Embryonic Stem Cells. Stem Cell Reports. 2018 Jan 24. pii: S2213-6711(17)30571-4. doi: 10.1016/j.stemcr.2017.12.023.
	pX458-sgRNA_Ago2_3	AGO2 (Mus musculus)	Mammalian Expression, CRISPR	Ciaudo	Argonaute 2 Is Required for Extra-embryonic Endoderm Differentiation of Mouse Embryonic Stem Cells. Stem Cell Reports. 2018 Jan 24. pii: S2213-6711(17)30571-4. doi: 10.1016/j.stemcr.2017.12.023.
	pX458-sgRNA_Ago2_4	AGO2 (Mus musculus)	Mammalian Expression, CRISPR	Ciaudo	Argonaute 2 Is Required for Extra-embryonic Endoderm Differentiation of Mouse Embryonic Stem Cells. Stem Cell Reports. 2018 Jan 24. pii: S2213-6711(17)30571-4. doi: 10.1016/j.stemcr.2017.12.023.
	pX458-sgRNA_Ago1_1	AGO1 (Mus musculus)	Mammalian Expression, CRISPR	Ciaudo	Argonaute 2 Is Required for Extra-embryonic Endoderm Differentiation of Mouse Embryonic Stem Cells. Stem Cell Reports. 2018 Jan 24. pii: S2213-6711(17)30571-4. doi: 10.1016/j.stemcr.2017.12.023.
	pX458-sgRNA_Ago1_2	AGO1 (Mus musculus)	Mammalian Expression, CRISPR	Ciaudo	Argonaute 2 Is Required for Extra-embryonic Endoderm Differentiation of Mouse Embryonic Stem Cells. Stem Cell Reports. 2018 Jan 24. pii: S2213-6711(17)30571-4. doi: 10.1016/j.stemcr.2017.12.023.
	pX458-sgRNA_Ago1_3	AGO1 (Mus musculus)	Mammalian Expression, CRISPR	Ciaudo	Argonaute 2 Is Required for Extra-embryonic Endoderm Differentiation of Mouse Embryonic Stem Cells. Stem Cell Reports. 2018 Jan 24. pii: S2213-6711(17)30571-4. doi: 10.1016/j.stemcr.2017.12.023.
	pX458-sgRNA_Ago1_4	AGO1 (Mus musculus)	Mammalian Expression, CRISPR	Ciaudo	Argonaute 2 Is Required for Extra-embryonic Endoderm Differentiation of Mouse Embryonic Stem Cells. Stem Cell Reports. 2018 Jan 24. pii: S2213-6711(17)30571-4. doi: 10.1016/j.stemcr.2017.12.023.
	pNICKclos1.0	Cas9 nickase (Other), sGRNA to pyrE	E.coli-clostridium shuttle vector	Yang	CRISPR-based genome editing and expression control systems in Clostridium acetobutylicum and Clostridium beijerinckii. Biotechnol J. 2016 May 23. doi: 10.1002/biot.201600053.
	pdCASclos	dCas9 (Other), sgRNA to spo0A	E.coli-clostridium shuttle vector	Yang	CRISPR-based genome editing and expression control systems in Clostridium acetobutylicum and Clostridium beijerinckii. Biotechnol J. 2016 May 23. doi: 10.1002/biot.201600053.
	B51	gRNA (Mus musculus)	CRISPR	Cepko	A gene regulatory network controls the binary fate decision of rod and bipolar cells in the vertebrate retina. Dev Cell. 2014 Sep 8;30(5):513-27. doi: 10.1016/j.devcel.2014.07.018. Epub 2014 Aug 21.
	B53	gRNA (Mus musculus)	CRISPR	Cepko	A gene regulatory network controls the binary fate decision of rod and bipolar cells in the vertebrate retina. Dev Cell. 2014 Sep 8;30(5):513-27. doi: 10.1016/j.devcel.2014.07.018. Epub 2014 Aug 21.
	B31	gRNA (Mus musculus)	CRISPR	Cepko	A gene regulatory network controls the binary fate decision of rod and bipolar cells in the vertebrate retina. Dev Cell. 2014 Sep 8;30(5):513-27. doi: 10.1016/j.devcel.2014.07.018. Epub 2014 Aug 21.
	B33	gRNA (Mus musculus)	CRISPR	Cepko	A gene regulatory network controls the binary fate decision of rod and bipolar cells in the vertebrate retina. Dev Cell. 2014 Sep 8;30(5):513-27. doi: 10.1016/j.devcel.2014.07.018. Epub 2014 Aug 21.
	pAL-pgi_T	sgRNA pgi (T)	Bacterial Expression, CRISPR, Synthetic Biology	Lu	Corynebacterium glutamicum Metabolic Engineering with CRISPR Interference (CRISPRi). ACS Synth Biol. 2016 Feb 16.
	pAL-pgi_NT	sgRNA pgi (NT) (Synthetic)	Bacterial Expression, CRISPR, Synthetic Biology	Lu	Corynebacterium glutamicum Metabolic Engineering with CRISPR Interference (CRISPRi). ACS Synth Biol. 2016 Feb 16.
	pAL-pck_T	sgRNA pck (T) (Synthetic)	Bacterial Expression, CRISPR, Synthetic Biology	Lu	Corynebacterium glutamicum Metabolic Engineering with CRISPR Interference (CRISPRi). ACS Synth Biol. 2016 Feb 16.
	pAL-pck_NT	sgRNA pck (NT) (Synthetic)	Bacterial Expression, CRISPR, Synthetic Biology	Lu	Corynebacterium glutamicum Metabolic Engineering with CRISPR Interference (CRISPRi). ACS Synth Biol. 2016 Feb 16.
	pAL-pyk_T	sgRNA pyk (T) (Synthetic)	Bacterial Expression, CRISPR, Synthetic Biology	Lu	Corynebacterium glutamicum Metabolic Engineering with CRISPR Interference (CRISPRi). ACS Synth Biol. 2016 Feb 16.
	pAL-pyk_NT	sgRNA pyk (NT) (Synthetic)	Bacterial Expression, CRISPR	Lu	Corynebacterium glutamicum Metabolic Engineering with CRISPR Interference (CRISPRi). ACS Synth Biol. 2016 Feb 16.
	pAL-rfp_T	sgRNA rfp (T) (Synthetic)	Bacterial Expression, Synthetic Biology	Lu	Corynebacterium glutamicum Metabolic Engineering with CRISPR Interference (CRISPRi). ACS Synth Biol. 2016 Feb 16.
	pLCKO_LacZ_sgRNA	LacZ sgRNA (Other)	Mammalian Expression, Lentiviral, CRISPR	Moffat	High-Resolution CRISPR Screens Reveal Fitness Genes and Genotype-Specific Cancer Liabilities. Cell. 2015 Dec 3;163(6):1515-26. doi: 10.1016/j.cell.2015.11.015. Epub 2015 Nov 25.
	pLCKO_PSMD1_sgRNA_1	PSMD1 sgRNA (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Moffat	High-Resolution CRISPR Screens Reveal Fitness Genes and Genotype-Specific Cancer Liabilities. Cell. 2015 Dec 3;163(6):1515-26. doi: 10.1016/j.cell.2015.11.015. Epub 2015 Nov 25.
	pLCKO_PSMD1_sgRNA_5	PSMD1 sgRNA (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Moffat	High-Resolution CRISPR Screens Reveal Fitness Genes and Genotype-Specific Cancer Liabilities. Cell. 2015 Dec 3;163(6):1515-26. doi: 10.1016/j.cell.2015.11.015. Epub 2015 Nov 25.
	pLCKO_PSMB2_sgRNA_1	PSMB2 sgRNA (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Moffat	High-Resolution CRISPR Screens Reveal Fitness Genes and Genotype-Specific Cancer Liabilities. Cell. 2015 Dec 3;163(6):1515-26. doi: 10.1016/j.cell.2015.11.015. Epub 2015 Nov 25.
	pLCKO_PSMB2_sgRNA_5	PSMB2 sgRNA (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Moffat	High-Resolution CRISPR Screens Reveal Fitness Genes and Genotype-Specific Cancer Liabilities. Cell. 2015 Dec 3;163(6):1515-26. doi: 10.1016/j.cell.2015.11.015. Epub 2015 Nov 25.
	pLCKO_EIF3D_sgRNA_1	EIF3D sgRNA (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Moffat	High-Resolution CRISPR Screens Reveal Fitness Genes and Genotype-Specific Cancer Liabilities. Cell. 2015 Dec 3;163(6):1515-26. doi: 10.1016/j.cell.2015.11.015. Epub 2015 Nov 25.
	pLCKO_EIF3D_sgRNA_4	EIF3D sgRNA (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Moffat	High-Resolution CRISPR Screens Reveal Fitness Genes and Genotype-Specific Cancer Liabilities. Cell. 2015 Dec 3;163(6):1515-26. doi: 10.1016/j.cell.2015.11.015. Epub 2015 Nov 25.
	pLCKO_Luciferase_sgRNA	Luciferase sgRNA (Other)	Mammalian Expression, Lentiviral, CRISPR	Moffat	High-Resolution CRISPR Screens Reveal Fitness Genes and Genotype-Specific Cancer Liabilities. Cell. 2015 Dec 3;163(6):1515-26. doi: 10.1016/j.cell.2015.11.015. Epub 2015 Nov 25.
	pMOD8A-iRGR-r11	RGR-r11 (Synthetic)	Yeast Expression, CRISPR, Synthetic Biology	Klavins	Robust digital logic circuits in eukaryotic cells with CRISPR/dCas9 NOR gates bioRxiv 041871
	pMOD8A-RGR-r10	RGR-r10 (Synthetic)	Yeast Expression, CRISPR, Synthetic Biology	Klavins	Robust digital logic circuits in eukaryotic cells with CRISPR/dCas9 NOR gates bioRxiv 041871
	pX462-hPRKAA1-gRNA_A	human AMPK alpha 1 exon1 gRNA (Homo sapiens)	CRISPR	Shaw	Metabolism. AMP-activated protein kinase mediates mitochondrial fission in response to energy stress. Science. 2016 Jan 15;351(6270):275-81. doi: 10.1126/science.aab4138.
	pX462-hPRKAA1-gRNA_B	human AMPK alpha 1 exon1 gRNA (Homo sapiens)	CRISPR	Shaw	Metabolism. AMP-activated protein kinase mediates mitochondrial fission in response to energy stress. Science. 2016 Jan 15;351(6270):275-81. doi: 10.1126/science.aab4138.
	pX462-hPRKAA2-gRNA_A	human AMPK alpha 2 exon1 gRNA (Homo sapiens)	CRISPR	Shaw	Metabolism. AMP-activated protein kinase mediates mitochondrial fission in response to energy stress. Science. 2016 Jan 15;351(6270):275-81. doi: 10.1126/science.aab4138.
	pX462-hPRKAA2-gRNA_B	human AMPK alpha 2 exon1 gRNA (Homo sapiens)	CRISPR	Shaw	Metabolism. AMP-activated protein kinase mediates mitochondrial fission in response to energy stress. Science. 2016 Jan 15;351(6270):275-81. doi: 10.1126/science.aab4138.
	pUC57-sgDedd-1	Dedd (Mus musculus)	Mammalian Expression, CRISPR	Arck	pUC57-sgDedd-1 (unpublished)
	pUC57-sgDedd-2	Dedd (Mus musculus)	Mammalian Expression, CRISPR	Arck	pUC57-sgDedd-1 (unpublished)
	pBluescriptSKII+ U6-sgRNA(F+E) ACTB	U6 promoter driving sgRNA targeting the 3'UTR of ACTB mRNA	Mammalian Expression, CRISPR	Yeo	Programmable RNA Tracking in Live Cells with CRISPR/Cas9. Cell. 2016 Mar 16. pii: S0092-8674(16)30204-5. doi: 10.1016/j.cell.2016.02.054.
	pBluescriptSKII+ U6-sgRNA(F+E) TFRC	U6 promoter driving sgRNA targeting the 3'UTR of TFRC mRNA	Mammalian Expression, CRISPR	Yeo	Programmable RNA Tracking in Live Cells with CRISPR/Cas9. Cell. 2016 Mar 16. pii: S0092-8674(16)30204-5. doi: 10.1016/j.cell.2016.02.054.
	pLC-EGFP-RIP1	RIPK1 sgRNA (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Bornhauser	Activation of concurrent apoptosis and necroptosis by SMAC mimetics for the treatment of refractory and relapsed ALL. Sci Transl Med. 2016 May 18;8(339):339ra70. doi: 10.1126/scitranslmed.aad2986.
	pLC-RFP657-CASP8	CASP8 sgRNA (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Bornhauser	Activation of concurrent apoptosis and necroptosis by SMAC mimetics for the treatment of refractory and relapsed ALL. Sci Transl Med. 2016 May 18;8(339):339ra70. doi: 10.1126/scitranslmed.aad2986.
	pLC-EGFP-RIP3	RIP3 sgRNA (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Bornhauser	Activation of concurrent apoptosis and necroptosis by SMAC mimetics for the treatment of refractory and relapsed ALL. Sci Transl Med. 2016 May 18;8(339):339ra70. doi: 10.1126/scitranslmed.aad2986.
	pLC-BFP-FADD	FADD sgRNA (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Bornhauser	Activation of concurrent apoptosis and necroptosis by SMAC mimetics for the treatment of refractory and relapsed ALL. Sci Transl Med. 2016 May 18;8(339):339ra70. doi: 10.1126/scitranslmed.aad2986.
	pLC-mCherry-MLKL	MLKL sgRNA (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Bornhauser	Activation of concurrent apoptosis and necroptosis by SMAC mimetics for the treatment of refractory and relapsed ALL. Sci Transl Med. 2016 May 18;8(339):339ra70. doi: 10.1126/scitranslmed.aad2986.
	NFATc2-CRISPR #1 (PX458-EF1a-pSpCas9(BB)-2A-GFP)	sgRNA against NFATc2 (Homo sapiens)	CRISPR	Baumgrass	Identification of Novel Nuclear Factor of Activated T Cells (NFAT)-Associated Proteins in T cells. J Biol Chem. 2016 Sep 16. pii: jbc.M116.739326.
	NFATc2-CRISPR #2 (PX458-EF1a-pSpCas9(BB)-2A-GFP)	sgRNA against NFATc2 (Homo sapiens)	CRISPR	Baumgrass	Identification of Novel Nuclear Factor of Activated T Cells (NFAT)-Associated Proteins in T cells. J Biol Chem. 2016 Sep 16. pii: jbc.M116.739326.
	NFATc2-CRISPR-Nick 1/2 (PX461-EF1a-pSpCas9n(BB)-2A-GFP)	sgRNA against NFATc2 (Homo sapiens)	CRISPR	Baumgrass	Identification of Novel Nuclear Factor of Activated T Cells (NFAT)-Associated Proteins in T cells. J Biol Chem. 2016 Sep 16. pii: jbc.M116.739326.
	NFATc2-CRISPR-Nick 2/2 (PX461-EF1a-pSpCas9n(BB)-2A-GFP)	sgRNA against NFATc2 (Homo sapiens)	CRISPR	Baumgrass	Identification of Novel Nuclear Factor of Activated T Cells (NFAT)-Associated Proteins in T cells. J Biol Chem. 2016 Sep 16. pii: jbc.M116.739326.
	NFATc1-CRISPR #1 (PX458-EF1a-pSpCas9(BB)-2A-GFP)	sgRNA against NFATc1 (Homo sapiens)	CRISPR	Baumgrass	Identification of Novel Nuclear Factor of Activated T Cells (NFAT)-Associated Proteins in T cells. J Biol Chem. 2016 Sep 16. pii: jbc.M116.739326.
	NFATc1-CRISPR #2 (PX458-EF1a-pSpCas9(BB)-2A-GFP)	sgRNA against NFATc1 (Homo sapiens)	CRISPR	Baumgrass	Identification of Novel Nuclear Factor of Activated T Cells (NFAT)-Associated Proteins in T cells. J Biol Chem. 2016 Sep 16. pii: jbc.M116.739326.
	NFATc1-CRISPR-Nick 1/2 (PX461-EF1a-pSpCas9n(BB)-2A-GFP)	sgRNA against NFATc1 (Homo sapiens)	CRISPR	Baumgrass	Identification of Novel Nuclear Factor of Activated T Cells (NFAT)-Associated Proteins in T cells. J Biol Chem. 2016 Sep 16. pii: jbc.M116.739326.
	NFATc1-CRISPR-Nick 2/2 (PX461-EF1a-pSpCas9n(BB)-2A-GFP)	sgRNA against NFATc1 (Homo sapiens)	CRISPR	Baumgrass	Identification of Novel Nuclear Factor of Activated T Cells (NFAT)-Associated Proteins in T cells. J Biol Chem. 2016 Sep 16. pii: jbc.M116.739326.
	Ikaros-CRISPR #1 (PX458-EF1a-pSpCas9(BB)-2A-GFP)	sgRNA against human Ikaros (Homo sapiens)	CRISPR	Baumgrass	Identification of Novel Nuclear Factor of Activated T Cells (NFAT)-Associated Proteins in T cells. J Biol Chem. 2016 Sep 16. pii: jbc.M116.739326.
	Ikaros-CRISPR #2 (PX458-EF1a-pSpCas9(BB)-2A-GFP)	sgRNA against human Ikaros (Homo sapiens)	CRISPR	Baumgrass	Identification of Novel Nuclear Factor of Activated T Cells (NFAT)-Associated Proteins in T cells. J Biol Chem. 2016 Sep 16. pii: jbc.M116.739326.
	Ikaros-CRISPR-Nick 1/2 (PX461-EF1a-pSpCas9n(BB)-2A-GFP)	sgRNA against human Ikaros (Homo sapiens)	CRISPR	Baumgrass	Identification of Novel Nuclear Factor of Activated T Cells (NFAT)-Associated Proteins in T cells. J Biol Chem. 2016 Sep 16. pii: jbc.M116.739326.
	Ikaros-CRISPR-Nick2/2 (PX461-EF1a-pSpCas9n(BB)-2A-GFP)	sgRNA against human Ikaros (Homo sapiens)	CRISPR	Baumgrass	Identification of Novel Nuclear Factor of Activated T Cells (NFAT)-Associated Proteins in T cells. J Biol Chem. 2016 Sep 16. pii: jbc.M116.739326.
	Helios-CRISPR #1 (PX458-EF1a-pSpCas9(BB)-2A-GFP)	sgRNA against human Helios (Homo sapiens)	CRISPR	Baumgrass	Identification of Novel Nuclear Factor of Activated T Cells (NFAT)-Associated Proteins in T cells. J Biol Chem. 2016 Sep 16. pii: jbc.M116.739326.
	Helios-CRISPR-Nick 1/2 (PX461-EF1a-pSpCas9n(BB)-2A-GFP)	sgRNA against human Helios (Homo sapiens)	CRISPR	Baumgrass	Identification of Novel Nuclear Factor of Activated T Cells (NFAT)-Associated Proteins in T cells. J Biol Chem. 2016 Sep 16. pii: jbc.M116.739326.
	Helios-CRISPR-Nick 2/2 (PX461-EF1a-pSpCas9n(BB)-2A-GFP)	sgRNA against human Helios (Homo sapiens)	CRISPR	Baumgrass	Identification of Novel Nuclear Factor of Activated T Cells (NFAT)-Associated Proteins in T cells. J Biol Chem. 2016 Sep 16. pii: jbc.M116.739326.
	pLH-sgRNA1	sgRNA1 (Synthetic)	Mammalian Expression, Lentiviral, CRISPR	Pederson	Multiplexed labeling of genomic loci with dCas9 and engineered sgRNAs using CRISPRainbow. Nat Biotechnol. 2016 Apr 18. doi: 10.1038/nbt.3526.
	pLH-sgRNA1-2XMS2	sgRNA1-2XMS2 (Synthetic)	Mammalian Expression, Lentiviral, CRISPR	Pederson	Multiplexed labeling of genomic loci with dCas9 and engineered sgRNAs using CRISPRainbow. Nat Biotechnol. 2016 Apr 18. doi: 10.1038/nbt.3526.
	pLH-sgRNA1-2XPP7	sgRNA1-2XPP7 (Synthetic)	Mammalian Expression, Lentiviral, CRISPR	Pederson	Multiplexed labeling of genomic loci with dCas9 and engineered sgRNAs using CRISPRainbow. Nat Biotechnol. 2016 Apr 18. doi: 10.1038/nbt.3526.
	pLH-sgRNA1-2XboxB	sgRNA1-2XboxB (Synthetic)	Mammalian Expression, Lentiviral, CRISPR	Pederson	Multiplexed labeling of genomic loci with dCas9 and engineered sgRNAs using CRISPRainbow. Nat Biotechnol. 2016 Apr 18. doi: 10.1038/nbt.3526.
	pLH-sgRNA1-MS2-PP7	sgRNA1-2XMS2-PP7 (Synthetic)	Mammalian Expression, Lentiviral, CRISPR	Pederson	Multiplexed labeling of genomic loci with dCas9 and engineered sgRNAs using CRISPRainbow. Nat Biotechnol. 2016 Apr 18. doi: 10.1038/nbt.3526.
	pLH-sgRNA1-PP7-boxB	sgRNA1-2XPP7-boxB (Synthetic)	Mammalian Expression, Lentiviral, CRISPR	Pederson	Multiplexed labeling of genomic loci with dCas9 and engineered sgRNAs using CRISPRainbow. Nat Biotechnol. 2016 Apr 18. doi: 10.1038/nbt.3526.
	pLH-sgRNA1-boxB-MS2	sgRNA1-boxB-MS2 (Synthetic)	Mammalian Expression, Lentiviral, CRISPR	Pederson	Multiplexed labeling of genomic loci with dCas9 and engineered sgRNAs using CRISPRainbow. Nat Biotechnol. 2016 Apr 18. doi: 10.1038/nbt.3526.
	pLH-sgRNA1-boxB-MS2-PP7	sgRNA1-boxB-MS2-PP7 (Synthetic)	Mammalian Expression, Lentiviral, CRISPR	Pederson	Multiplexed labeling of genomic loci with dCas9 and engineered sgRNAs using CRISPRainbow. Nat Biotechnol. 2016 Apr 18. doi: 10.1038/nbt.3526.
	NLK gRNA (BRDN0001146865)	NLK (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	NLK gRNA (BRDN0001147584)	NLK (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	NLK gRNA (BRDN0001147680)	NLK (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	ACVR2B gRNA (BRDN0001145393)	ACVR2B (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	ACVR2B gRNA (BRDN0001147208)	ACVR2B (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	ACVR2B gRNA (BRDN0001149036)	ACVR2B (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	STK10 gRNA (BRDN0001148022)	STK10 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	STK10 gRNA (BRDN0001149099)	STK10 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	STK10 gRNA (BRDN0001162510)	STK10 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	STK10 gRNA (BRDN0001162500)	STK10 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	PDGFRA gRNA (BRDN0001145116)	PDGFRA (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	PDGFRA gRNA (BRDN0001145903)	PDGFRA (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	PDGFRA gRNA (BRDN0001148373)	PDGFRA (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	STRADB gRNA (BRDN0001146450)	STRADB (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	STRADB gRNA (BRDN0001146838)	STRADB (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	STRADB gRNA (BRDN0001148940)	STRADB (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	RPS6KA1 gRNA (BRDN0001145974)	RPS6KA1 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	RPS6KA1 gRNA (BRDN0001148103)	RPS6KA1 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	RPS6KA1 gRNA (BRDN0001148481)	RPS6KA1 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	AKT1 gRNA (BRDN0001146935)	AKT1 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	AKT1 gRNA (BRDN0001149266)	AKT1 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	AKT1 gRNA (BRDN0001149414)	AKT1 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	BCKDK gRNA (BRDN0001147783)	BCKDK (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	BCKDK gRNA (BRDN0001147891)	BCKDK (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	BCKDK gRNA (BRDN0001149253)	BCKDK (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	INSR gRNA (BRDN0001145804)	INSR (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	INSR gRNA (BRDN0001148089)	INSR (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	INSR gRNA (BRDN0001149243)	INSR (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	MAP3K2 gRNA (BRDN0001145545)	MAP3K2 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	MAP3K2 gRNA (BRDN0001146616)	MAP3K2 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	MAP3K2 gRNA (BRDN0001147901)	MAP3K2 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	MKNK1 gRNA (BRDN0001146808)	MKNK1 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	MKNK1 gRNA (BRDN0001146937)	MKNK1 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	MKNK1 gRNA (BRDN0001148208)	MKNK1 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	PRKACA gRNA (BRDN0001144986)	PRKACA (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	PRKACA gRNA (BRDN0001145395)	PRKACA (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	PRKACA gRNA (BRDN0001145877)	PRKACA (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	PRKACA gRNA (BRDN0001148333)	PRKACA (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	DYRK4 gRNA (BRDN0001145834)	DYRK4 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	DYRK4 gRNA (BRDN0001147655)	DYRK4 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	DYRK4 gRNA (BRDN0001149224)	DYRK4 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	VRK1 gRNA (BRDN0001147568)	VRK1 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	VRK1 gRNA (BRDN0001148483)	VRK1 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	VRK1 gRNA (BRDN0001149207)	VRK1 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	VRK1 gRNA (BRDN0001149172)	VRK1 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	CAMKV gRNA (BRDN0001145768)	CAMKV (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	CAMKV gRNA (BRDN0001162514)	CAMKV (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	CAMKV gRNA (BRDN0001162507)	CAMKV (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	CAMKV gRNA (BRDN0001162273)	CAMKV (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	ETNK1 gRNA (BRDN0001147553)	ETNK1 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	ETNK1 gRNA (BRDN0001148309)	ETNK1 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	ETNK1 gRNA (BRDN0001148358)	ETNK1 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	RPS6KA6 gRNA (BRDN0001145731)	RPS6KA6 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	RPS6KA6 gRNA (BRDN0001146818)	RPS6KA6 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	RPS6KA6 gRNA (BRDN0001147018)	RPS6KA6 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	RPS6KA6 gRNA (BRDN0001147921)	RPS6KA6 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	NME1 gRNA (BRDN0001145504)	NME1,NME1-NME2 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	NME1 gRNA (BRDN0001146170)	NME1,NME1-NME2 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	NME1 gRNA (BRDN0001146284)	NME1,NME1-NME2 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	SGK3 gRNA (BRDN0001146019)	C8orf44-SGK3,SGK3 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	SGK3 gRNA (BRDN0001146281)	C8orf44-SGK3,SGK3 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	SGK3 gRNA (BRDN0001147184)	C8orf44-SGK3,SGK3 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	PTK2 gRNA (BRDN0001145734)	PTK2 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	PTK2 gRNA (BRDN0001146703)	PTK2 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	PTK2 gRNA (BRDN0001148426)	PTK2 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	DYRK3 gRNA (BRDN0001145321)	DYRK3 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	DYRK3 gRNA (BRDN0001145918)	DYRK3 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	DYRK3 gRNA (BRDN0001149001)	DYRK3 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	VRK3 gRNA (BRDN0001144937)	VRK3 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	VRK3 gRNA (BRDN0001145580)	VRK3 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	VRK3 gRNA (BRDN0001147251)	VRK3 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	VRK3 gRNA (BRDN0001148188)	VRK3 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	PRKCQ gRNA (BRDN0001144886)	PRKCQ (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	PRKCQ gRNA (BRDN0001145693)	PRKCQ (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	PRKCQ gRNA (BRDN0001147386)	PRKCQ (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	FLT1 gRNA (BRDN0001145335)	FLT1 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	FLT1 gRNA (BRDN0001145564)	FLT1 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	FLT1 gRNA (BRDN0001146782)	FLT1 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	FLT1 gRNA (BRDN0001147168)	FLT1 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	NEK11 gRNA (BRDN0001144720)	NEK11 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	NEK11 gRNA (BRDN0001145274)	NEK11 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	NEK11 gRNA (BRDN0001146419)	NEK11 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	NEK11 gRNA (BRDN0001147265)	NEK11 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	TBCK gRNA (BRDN0001147900)	TBCK (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	TBCK gRNA (BRDN0001148062)	TBCK (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	TBCK gRNA (BRDN0001149038)	TBCK (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	TBCK gRNA (BRDN0001149188)	TBCK (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	MYO3B gRNA (BRDN0001145051)	MYO3B (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	MYO3B gRNA (BRDN0001145445)	MYO3B (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	MYO3B gRNA (BRDN0001145615)	MYO3B (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	MYO3B gRNA (BRDN0001147808)	MYO3B (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	PBK gRNA (BRDN0001145450)	PBK (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	PBK gRNA (BRDN0001145489)	PBK (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	PBK gRNA (BRDN0001147308)	PBK (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	NUAK1 gRNA (BRDN0001145557)	NUAK1 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	NUAK1 gRNA (BRDN0001147733)	NUAK1 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	NUAK1 gRNA (BRDN0001162598)	NUAK1 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	NUAK1 gRNA (BRDN0001162525)	NUAK1 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	TTK gRNA (BRDN0001145152)	TTK (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	TTK gRNA (BRDN0001146504)	TTK (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	TTK gRNA (BRDN0001147779)	TTK (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	TNK2 gRNA (BRDN0001148153)	TNK2 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	TNK2 gRNA (BRDN0001148514)	TNK2 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	TNK2 gRNA (BRDN0001149049)	TNK2 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	TNK2 gRNA (BRDN0001149191)	TNK2 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	CSNK2A2 gRNA (BRDN0001144724)	CSNK2A2 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	CSNK2A2 gRNA (BRDN0001147190)	CSNK2A2 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	CSNK2A2 gRNA (BRDN0001148502)	CSNK2A2 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	EPHA1 gRNA (BRDN0001145712)	EPHA1 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	EPHA1 gRNA (BRDN0001147142)	EPHA1 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	EPHA1 gRNA (BRDN0001148678)	EPHA1 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	PIK3R4 gRNA (BRDN0001145205)	PIK3R4 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	PIK3R4 gRNA (BRDN0001145724)	PIK3R4 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	PIK3R4 gRNA (BRDN0001148885)	PIK3R4 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	PIK3R4 gRNA (BRDN0001148908)	PIK3R4 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	ZAK gRNA (BRDN0001145178)	ZAK (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	ZAK gRNA (BRDN0001146540)	ZAK (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	ZAK gRNA (BRDN0001146874)	ZAK (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	SRPK2 gRNA (BRDN0001145577)	SRPK2 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	SRPK2 gRNA (BRDN0001145583)	SRPK2 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	SRPK2 gRNA (BRDN0001146940)	SRPK2 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	TRIB2 gRNA (BRDN0001144754)	TRIB2 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	TRIB2 gRNA (BRDN0001148141)	TRIB2 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	TRIB2 gRNA (BRDN0001148332)	TRIB2 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	CDKL3 gRNA (BRDN0001146569)	CDKL3 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	CDKL3 gRNA (BRDN0001148232)	CDKL3 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	CDKL3 gRNA (BRDN0001148623)	CDKL3 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	DMPK gRNA (BRDN0001145527)	DMPK (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	DMPK gRNA (BRDN0001145673)	DMPK (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	DMPK gRNA (BRDN0001146276)	DMPK (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	DMPK gRNA (BRDN0001147274)	DMPK (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	RPS6KB1 gRNA (BRDN0001145729)	RPS6KB1 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	RPS6KB1 gRNA (BRDN0001145927)	RPS6KB1 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	RPS6KB1 gRNA (BRDN0001148368)	RPS6KB1 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	TLK2 gRNA (BRDN0001146448)	TLK2 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	TLK2 gRNA (BRDN0001147069)	TLK2 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	TLK2 gRNA (BRDN0001147939)	TLK2 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	MAPK15 gRNA (BRDN0001145787)	MAPK15 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	MAPK15 gRNA (BRDN0001148491)	MAPK15 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	MAPK15 gRNA (BRDN0001162376)	MAPK15 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	MAPK15 gRNA (BRDN0001162374)	MAPK15 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	WNK1 gRNA (BRDN0001146391)	WNK1 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	WNK1 gRNA (BRDN0001147437)	WNK1 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	WNK1 gRNA (BRDN0001148217)	WNK1 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	STK40 gRNA (BRDN0001147563)	STK40 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	STK40 gRNA (BRDN0001147746)	STK40 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	STK40 gRNA (BRDN0001149285)	STK40 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	STK40 gRNA (BRDN0001149509)	STK40 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	FLT4 gRNA (BRDN0001147158)	FLT4 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	FLT4 gRNA (BRDN0001147213)	FLT4 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	FLT4 gRNA (BRDN0001149037)	FLT4 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	NEK10 gRNA (BRDN0001146401)	NEK10 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	NEK10 gRNA (BRDN0001149193)	NEK10 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	NEK10 gRNA (BRDN0001162522)	NEK10 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	NEK10 gRNA (BRDN0001162335)	NEK10 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	EIF2AK2 gRNA (BRDN0001146342)	EIF2AK2 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	EIF2AK2 gRNA (BRDN0001148577)	EIF2AK2 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	EIF2AK2 gRNA (BRDN0001148668)	EIF2AK2 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	HSPB8 gRNA (BRDN0001145453)	HSPB8 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	HSPB8 gRNA (BRDN0001146985)	HSPB8 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	HSPB8 gRNA (BRDN0001147486)	HSPB8 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	CDK6 gRNA (BRDN0001145692)	CDK6 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	CDK6 gRNA (BRDN0001146904)	CDK6 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	CDK6 gRNA (BRDN0001147855)	CDK6 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	CDK6 gRNA (BRDN0001148604)	CDK6 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	TSSK2 gRNA (BRDN0001144840)	TSSK2 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	TSSK2 gRNA (BRDN0001148461)	TSSK2 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	TSSK2 gRNA (BRDN0001148488)	TSSK2 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	SRC gRNA (BRDN0001145402)	SRC (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	SRC gRNA (BRDN0001146185)	SRC (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	SRC gRNA (BRDN0001149457)	SRC (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	BMX gRNA (BRDN0001146364)	BMX (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	BMX gRNA (BRDN0001146561)	BMX (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	BMX gRNA (BRDN0001147055)	BMX (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	WNK4 gRNA (BRDN0001147338)	WNK4 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	WNK4 gRNA (BRDN0001147390)	WNK4 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	WNK4 gRNA (BRDN0001148891)	WNK4 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	WNK4 gRNA (BRDN0001148888)	WNK4 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	ARAF gRNA (BRDN0001147899)	ARAF (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	ARAF gRNA (BRDN0001148242)	ARAF (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	ARAF gRNA (BRDN0001148605)	ARAF (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	NEK9 gRNA (BRDN0001146898)	NEK9 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	IRAK4 gRNA (BRDN0001146161)	IRAK4 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	IRAK4 gRNA (BRDN0001147550)	IRAK4 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	IRAK4 gRNA (BRDN0001149331)	IRAK4 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	MELK gRNA (BRDN0001144817)	MELK (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	MELK gRNA (BRDN0001145853)	MELK (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	MELK gRNA (BRDN0001146713)	MELK (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	WNK2 gRNA (BRDN0001148439)	WNK2 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	WNK2 gRNA (BRDN0001162416)	WNK2 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	WNK2 gRNA (BRDN0001162408)	WNK2 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	WNK2 gRNA (BRDN0001162262)	WNK2 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	PRKAB1 gRNA (BRDN0001146320)	PRKAB1 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	PRKAB1 gRNA (BRDN0001146367)	PRKAB1 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	PRKAB1 gRNA (BRDN0001162435)	PRKAB1 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	PRKAB1 gRNA (BRDN0001162244)	PRKAB1 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	PAK3 gRNA (BRDN0001148041)	PAK3 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	PAK3 gRNA (BRDN0001148181)	PAK3 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	PAK3 gRNA (BRDN0001148485)	PAK3 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	ADRBK1 gRNA (BRDN0001144871)	ADRBK1 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	ADRBK1 gRNA (BRDN0001145957)	ADRBK1 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	ADRBK1 gRNA (BRDN0001147663)	ADRBK1 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	ADRBK1 gRNA (BRDN0001147919)	ADRBK1 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	PDK2 gRNA (BRDN0001145761)	PDK2 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	PDK2 gRNA (BRDN0001146093)	PDK2 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	PDK2 gRNA (BRDN0001148391)	PDK2 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	EGFR gRNA (BRDN0001146570)	EGFR (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	EGFR gRNA (BRDN0001148919)	EGFR (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	EGFR gRNA (BRDN0001149231)	EGFR (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	EGFR gRNA (BRDN0001149464)	EGFR (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	MAP3K7 gRNA (BRDN0001145981)	MAP3K7 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	MAP3K7 gRNA (BRDN0001148020)	MAP3K7 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	MAP3K7 gRNA (BRDN0001148226)	MAP3K7 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	STYK1 gRNA (BRDN0001145785)	STYK1 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	STYK1 gRNA (BRDN0001146810)	STYK1 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	STYK1 gRNA (BRDN0001146987)	STYK1 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	DCLK2 gRNA (BRDN0001145364)	DCLK2 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	DCLK2 gRNA (BRDN0001162232)	DCLK2 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	DCLK2 gRNA (BRDN0001162228)	DCLK2 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	DCLK2 gRNA (BRDN0001162199)	DCLK2 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	GRK6 gRNA (BRDN0001145803)	GRK6 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	GRK6 gRNA (BRDN0001146204)	GRK6 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	GRK6 gRNA (BRDN0001146643)	GRK6 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	GRK6 gRNA (BRDN0001147105)	GRK6 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	CDK20 gRNA (BRDN0001144911)	CDK20 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	CDK20 gRNA (BRDN0001146337)	CDK20 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	CDK20 gRNA (BRDN0001146285)	CDK20 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	CDK20 gRNA (BRDN0001147842)	CDK20 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	MAP4K5 gRNA (BRDN0001145246)	MAP4K5 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	MAP4K5 gRNA (BRDN0001149200)	MAP4K5 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	MAP4K5 gRNA (BRDN0001149337)	MAP4K5 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	PAK7 gRNA (BRDN0001146752)	PAK7 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	PAK7 gRNA (BRDN0001147115)	PAK7 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	PAK7 gRNA (BRDN0001149524)	PAK7 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	LRRK1 gRNA (BRDN0001148276)	LRRK1 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	LRRK1 gRNA (BRDN0001148931)	LRRK1 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	LRRK1 gRNA (BRDN0001149233)	LRRK1 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	FGR gRNA (BRDN0001146726)	FGR (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	FGR gRNA (BRDN0001147671)	FGR (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	FGR gRNA (BRDN0001147903)	FGR (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	TEK gRNA (BRDN0001146784)	TEK (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	TEK gRNA (BRDN0001148630)	TEK (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	TEK gRNA (BRDN0001148973)	TEK (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	TEK gRNA (BRDN0001149392)	TEK (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	JAK2 gRNA (BRDN0001146541)	JAK2 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	JAK2 gRNA (BRDN0001147052)	JAK2 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	JAK2 gRNA (BRDN0001149125)	JAK2 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	FGFR3 gRNA (BRDN0001146639)	FGFR3 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	FGFR3 gRNA (BRDN0001147930)	FGFR3 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	FGFR3 gRNA (BRDN0001148456)	FGFR3 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	RPS6KA2 gRNA (BRDN0001147334)	RPS6KA2 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	RPS6KA2 gRNA (BRDN0001148104)	RPS6KA2 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	RPS6KA2 gRNA (BRDN0001148851)	RPS6KA2 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	RPS6KA2 gRNA (BRDN0001149230)	RPS6KA2 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	ULK1 gRNA (BRDN0001145044)	ULK1 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	ULK1 gRNA (BRDN0001145850)	ULK1 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	ULK1 gRNA (BRDN0001147908)	ULK1 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	PIK3C2G gRNA (BRDN0001148187)	PIK3C2G (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	PIK3C2G gRNA (BRDN0001148335)	PIK3C2G (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	PIK3C2G gRNA (BRDN0001149539)	PIK3C2G (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	ADK gRNA (BRDN0001146463)	ADK (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	ADK gRNA (BRDN0001146410)	ADK (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	ADK gRNA (BRDN0001146622)	ADK (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	ADK gRNA (BRDN0001147557)	ADK (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	CDC42BPA gRNA (BRDN0001145566)	CDC42BPA (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	CDC42BPA gRNA (BRDN0001146626)	CDC42BPA (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	CDC42BPA gRNA (BRDN0001147767)	CDC42BPA (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	BLK gRNA (BRDN0001146701)	BLK (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	BLK gRNA (BRDN0001147641)	BLK (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	BLK gRNA (BRDN0001149130)	BLK (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	BLK gRNA (BRDN0001149400)	BLK (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	CAMK2A gRNA (BRDN0001149371)	CAMK2A (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	SIK3 gRNA (BRDN0001144776)	SIK3 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	SIK3 gRNA (BRDN0001148297)	SIK3 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	SIK3 gRNA (BRDN0001148836)	SIK3 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	SIK3 gRNA (BRDN0001149197)	SIK3 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	PIK3R5 gRNA (BRDN0001145982)	PIK3R5 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	PIK3R5 gRNA (BRDN0001146332)	PIK3R5 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	PIK3R5 gRNA (BRDN0001146461)	PIK3R5 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	CALM1 gRNA (BRDN0001145447)	CALM1 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	CALM1 gRNA (BRDN0001145487)	CALM1 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	CALM1 gRNA (BRDN0001146464)	CALM1 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	CALM1 gRNA (BRDN0001148506)	CALM1 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	UCK2 gRNA (BRDN0001145127)	UCK2 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	UCK2 gRNA (BRDN0001145769)	UCK2 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	UCK2 gRNA (BRDN0001146352)	UCK2 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	UCK2 gRNA (BRDN0001146876)	UCK2 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	SMG1 gRNA (BRDN0001144779)	SMG1 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	SMG1 gRNA (BRDN0001146980)	SMG1 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	SMG1 gRNA (BRDN0001149395)	SMG1 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	SMG1 gRNA (BRDN0001149508)	SMG1 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	GK5 gRNA (BRDN0001146859)	GK5 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	GK5 gRNA (BRDN0001148146)	GK5 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	GK5 gRNA (BRDN0001149330)	GK5 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	MST1 gRNA (BRDN0001146525)	MST1 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	MST1 gRNA (BRDN0001147042)	MST1 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	MST1 gRNA (BRDN0001148968)	MST1 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	HK3 gRNA (BRDN0001146645)	HK3 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	HK3 gRNA (BRDN0001148398)	HK3 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	HK3 gRNA (BRDN0001149028)	HK3 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	HK3 gRNA (BRDN0001149156)	HK3 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	TKFC gRNA (BRDN0001144729)	TKFC (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	TKFC gRNA (BRDN0001146826)	TKFC (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	TKFC gRNA (BRDN0001148963)	TKFC (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	TKFC gRNA (BRDN0001149092)	TKFC (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	PLK5 gRNA (BRDN0001144735)	PLK5 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	PLK5 gRNA (BRDN0001145831)	PLK5 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	PLK5 gRNA (BRDN0001146558)	PLK5 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	PLK5 gRNA (BRDN0001147846)	PLK5 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	SGK223 gRNA (BRDN0001146707)	SGK223 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	SGK223 gRNA (BRDN0001147337)	SGK223 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	SGK223 gRNA (BRDN0001147947)	SGK223 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	GSAP gRNA (BRDN0001146551)	GSAP (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	GSAP gRNA (BRDN0001146681)	GSAP (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	GSAP gRNA (BRDN0001148675)	GSAP (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	SBK1 gRNA (BRDN0001145304)	SBK1 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	SBK1 gRNA (BRDN0001146823)	SBK1 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	SBK1 gRNA (BRDN0001148216)	SBK1 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	SBK1 gRNA (BRDN0001148223)	SBK1 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	TTBK1 gRNA (BRDN0001145252)	TTBK1 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	TTBK1 gRNA (BRDN0001146632)	TTBK1 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	TTBK1 gRNA (BRDN0001148007)	TTBK1 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	CMPK2 gRNA (BRDN0001144952)	CMPK2 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	CMPK2 gRNA (BRDN0001145007)	CMPK2 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	CMPK2 gRNA (BRDN0001147027)	CMPK2 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	PKDCC gRNA (BRDN0001144772)	PKDCC (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	PKDCC gRNA (BRDN0001144824)	PKDCC (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	PKDCC gRNA (BRDN0001145657)	PKDCC (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	PKDCC gRNA (BRDN0001148026)	PKDCC (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	STKLD1 gRNA (BRDN0001147552)	STKLD1 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	STKLD1 gRNA (BRDN0001148566)	STKLD1 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	STKLD1 gRNA (BRDN0001162456)	STKLD1 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	MYLK4 gRNA (BRDN0001145660)	MYLK4 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	MYLK4 gRNA (BRDN0001146621)	MYLK4 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	MYLK4 gRNA (BRDN0001146724)	MYLK4 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	COASY gRNA (BRDN0001145497)	COASY (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	COASY gRNA (BRDN0001145628)	COASY (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	COASY gRNA (BRDN0001147007)	COASY (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	COASY gRNA (BRDN0001147178)	COASY (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	PIP5KL1 gRNA (BRDN0001145139)	PIP5KL1 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	PIP5KL1 gRNA (BRDN0001146428)	PIP5KL1 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	PIP5KL1 gRNA (BRDN0001148279)	PIP5KL1 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	CD2 gRNA (BRDN0001145097)	CD2 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	CD2 gRNA (BRDN0001146027)	CD2 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	CD2 gRNA (BRDN0001146864)	CD2 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	CD2 gRNA (BRDN0001148522)	CD2 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	NMRK1 gRNA (BRDN0001144748)	NMRK1 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	NMRK1 gRNA (BRDN0001145652)	NMRK1 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	NMRK1 gRNA (BRDN0001148142)	NMRK1 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	MPP7 gRNA (BRDN0001145242)	MPP7 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	MPP7 gRNA (BRDN0001147517)	MPP7 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	MPP7 gRNA (BRDN0001149493)	MPP7 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	SNX16 gRNA (BRDN0001146480)	SNX16 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	SNX16 gRNA (BRDN0001147293)	SNX16 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	SNX16 gRNA (BRDN0001148051)	SNX16 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	PRPS1L1 gRNA (BRDN0001145013)	PRPS1L1 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	PRPS1L1 gRNA (BRDN0001146253)	PRPS1L1 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	PRPS1L1 gRNA (BRDN0001146979)	PRPS1L1 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	PRPS1L1 gRNA (BRDN0001147147)	PRPS1L1 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	DGKK gRNA (BRDN0001144811)	DGKK (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	DGKK gRNA (BRDN0001145466)	DGKK (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	DGKK gRNA (BRDN0001146124)	DGKK (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	ERN2 gRNA (BRDN0001145316)	ERN2 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	ERN2 gRNA (BRDN0001147211)	ERN2 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	ERN2 gRNA (BRDN0001162553)	ERN2 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	ERN2 gRNA (BRDN0001162277)	ERN2 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	TNIK gRNA (BRDN0001146045)	TNIK (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	TNIK gRNA (BRDN0001146101)	TNIK (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	TNIK gRNA (BRDN0001149026)	TNIK (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	TNIK gRNA (BRDN0001149184)	TNIK (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	PEAK1 gRNA (BRDN0001145890)	PEAK1 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	PEAK1 gRNA (BRDN0001147244)	PEAK1 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	PEAK1 gRNA (BRDN0001147666)	PEAK1 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	MEX3B gRNA (BRDN0001147189)	MEX3B (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	MEX3B gRNA (BRDN0001147925)	MEX3B (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	MEX3B gRNA (BRDN0001148319)	MEX3B (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	ATMIN gRNA (BRDN0001145377)	ATMIN (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	ATMIN gRNA (BRDN0001147857)	ATMIN (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	ATMIN gRNA (BRDN0001148346)	ATMIN (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	CIB4 gRNA (BRDN0001147449)	CIB4 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	CIB4 gRNA (BRDN0001149378)	CIB4 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	IDNK gRNA (BRDN0001146514)	IDNK (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	IDNK gRNA (BRDN0001147773)	IDNK (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	IDNK gRNA (BRDN0001148428)	IDNK (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	PIM3 gRNA (BRDN0001145296)	PIM3 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	PIM3 gRNA (BRDN0001145891)	PIM3 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	PIM3 gRNA (BRDN0001148886)	PIM3 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	PIM3 gRNA (BRDN0001149344)	PIM3 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	DCAKD gRNA (BRDN0001144969)	DCAKD (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	DCAKD gRNA (BRDN0001148499)	DCAKD (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	DCAKD gRNA (BRDN0001148979)	DCAKD (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	EIF2AK4 gRNA (BRDN0001145948)	EIF2AK4 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	EIF2AK4 gRNA (BRDN0001146469)	EIF2AK4 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	EIF2AK4 gRNA (BRDN0001147521)	EIF2AK4 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	CKMT1A gRNA (BRDN0001145999)	CKMT1A,CKMT1B (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	CKMT1A gRNA (BRDN0001149136)	CKMT1A,CKMT1B (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	CKMT1A gRNA (BRDN0001149109)	CKMT1A,CKMT1B (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	SBK2 gRNA (BRDN0001146871)	SBK2 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	SBK2 gRNA (BRDN0001147500)	SBK2 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	SBK2 gRNA (BRDN0001162195)	SBK2 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	SBK2 gRNA (BRDN0001162192)	SBK2 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	NME1-NME2 gRNA (BRDN0001145211)	NME1,NME1-NME2 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	NME1-NME2 gRNA (BRDN0001147480)	NME1,NME1-NME2 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	NME1-NME2 gRNA (BRDN0001162403)	NME1,NME1-NME2 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	NME1-NME2 gRNA (BRDN0001162367)	NME1,NME1-NME2 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	CDK11A gRNA (BRDN0001145793)	CDK11A (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	CDK11A gRNA (BRDN0001146824)	CDK11A (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	CDK11A gRNA (BRDN0001147032)	CDK11A (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	CDK11A gRNA (BRDN0001147530)	CDK11A (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	MORN2 gRNA (BRDN0001145801)	MORN2 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	MORN2 gRNA (BRDN0001146420)	MORN2 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	MORN2 gRNA (BRDN0001149432)	MORN2 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	WEE2 gRNA (BRDN0001146772)	WEE2 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	WEE2 gRNA (BRDN0001147038)	WEE2 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	WEE2 gRNA (BRDN0001148111)	WEE2 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	SBK3 gRNA (BRDN0001146759)	SBK3 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	SBK3 gRNA (BRDN0001147686)	SBK3 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	SBK3 gRNA (BRDN0001148159)	SBK3 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	FPGT-TNNI3K gRNA (BRDN0001145543)	FPGT-TNNI3K,TNNI3K (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	FPGT-TNNI3K gRNA (BRDN0001147862)	FPGT-TNNI3K,TNNI3K (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	FPGT-TNNI3K gRNA (BRDN0001147836)	FPGT-TNNI3K,TNNI3K (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	FPGT-TNNI3K gRNA (BRDN0001148036)	FPGT-TNNI3K,TNNI3K (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	C8orf44-SGK3 gRNA (BRDN0001146567)	C8orf44-SGK3,SGK3 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	C8orf44-SGK3 gRNA (BRDN0001147093)	C8orf44-SGK3,SGK3 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	C8orf44-SGK3 gRNA (BRDN0001148559)	C8orf44-SGK3,SGK3 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	ILK gRNA (BRDN0001147357)	ILK (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	ILK gRNA (BRDN0001146314)	ILK (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	ILK gRNA (BRDN0001144869)	ILK (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	STK11 gRNA (BRDN0001146880)	STK11 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	STK11 gRNA (BRDN0001146194)	STK11 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	STK11 gRNA (BRDN0001147319)	STK11 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	CDK12 gRNA (BRDN0001147156)	CDK12 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	CDK12 gRNA (BRDN0001147294)	CDK12 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	CDK12 gRNA (BRDN0001145847)	CDK12 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	BUB1B gRNA (BRDN0001148077)	BUB1B (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	BUB1B gRNA (BRDN0001146079)	BUB1B (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	BUB1B gRNA (BRDN0001147884)	BUB1B (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	MAP3K8 gRNA (BRDN0001146430)	MAP3K8 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	MAP3K8 gRNA (BRDN0001148662)	MAP3K8 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	MAP3K8 gRNA (BRDN0001146835)	MAP3K8 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	PRKCA gRNA (BRDN0001148197)	PRKCA (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	PRKCA gRNA (BRDN0001149352)	PRKCA (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	PRKCA gRNA (BRDN0001145588)	PRKCA (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	BMP2K gRNA (BRDN0001145305)	BMP2K (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	BMP2K gRNA (BRDN0001146223)	BMP2K (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	BMP2K gRNA (BRDN0001146802)	BMP2K (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	MOS gRNA (BRDN0001147046)	MOS (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	MOS gRNA (BRDN0001146861)	MOS (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	MOS gRNA (BRDN0001146393)	MOS (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	STK16 gRNA (BRDN0001146155)	STK16 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	STK16 gRNA (BRDN0001146199)	STK16 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	STK16 gRNA (BRDN0001145107)	STK16 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	MOK gRNA (BRDN0001145525)	MOK (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	MOK gRNA (BRDN0001145476)	MOK (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	MOK gRNA (BRDN0001148493)	MOK (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	HUNK gRNA (BRDN0001146355)	HUNK (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	HUNK gRNA (BRDN0001148239)	HUNK (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	HUNK gRNA (BRDN0001145500)	HUNK (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	RPS6KA3 gRNA (BRDN0001144943)	RPS6KA3 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	RPS6KA3 gRNA (BRDN0001148452)	RPS6KA3 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	RPS6KA3 gRNA (BRDN0001147852)	RPS6KA3 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	EPHB3 gRNA (BRDN0001146280)	EPHB3 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	EPHB3 gRNA (BRDN0001145322)	EPHB3 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	EPHB3 gRNA (BRDN0001147101)	EPHB3 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	MYLK3 gRNA (BRDN0001149300)	MYLK3 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	MYLK3 gRNA (BRDN0001146129)	MYLK3 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	MYLK3 gRNA (BRDN0001148087)	MYLK3 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	PRKD3 gRNA (BRDN0001146689)	PRKD3 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	PRKD3 gRNA (BRDN0001146046)	PRKD3 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	PRKD3 gRNA (BRDN0001146945)	PRKD3 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	PRKCB gRNA (BRDN0001148507)	PRKCB (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	PRKCB gRNA (BRDN0001144990)	PRKCB (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	PRKCB gRNA (BRDN0001145137)	PRKCB (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	STK25 gRNA (BRDN0001149116)	STK25 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	STK25 gRNA (BRDN0001148478)	STK25 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	STK25 gRNA (BRDN0001146374)	STK25 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	PRKACB gRNA (BRDN0001145037)	PRKACB (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	PRKACB gRNA (BRDN0001147109)	PRKACB (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	PRKACB gRNA (BRDN0001148118)	PRKACB (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	NTRK1 gRNA (BRDN0001147322)	NTRK1 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	NTRK1 gRNA (BRDN0001145717)	NTRK1 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	NTRK1 gRNA (BRDN0001148112)	NTRK1 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	MAPK3 gRNA (BRDN0001149014)	MAPK3 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	MAPK3 gRNA (BRDN0001147876)	MAPK3 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	MAPK3 gRNA (BRDN0001147667)	MAPK3 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	RIPK4 gRNA (BRDN0001147121)	RIPK4 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	RIPK4 gRNA (BRDN0001144783)	RIPK4 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	RIPK4 gRNA (BRDN0001146610)	RIPK4 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	LIMK2 gRNA (BRDN0001148526)	LIMK2 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	LIMK2 gRNA (BRDN0001147235)	LIMK2 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	LIMK2 gRNA (BRDN0001148972)	LIMK2 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	STK3 gRNA (BRDN0001147084)	STK3 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	STK3 gRNA (BRDN0001145644)	STK3 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	STK3 gRNA (BRDN0001145514)	STK3 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	RPS6KC1 gRNA (BRDN0001147352)	RPS6KC1 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	RPS6KC1 gRNA (BRDN0001148621)	RPS6KC1 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	RPS6KC1 gRNA (BRDN0001145776)	RPS6KC1 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	MAPK10 gRNA (BRDN0001148192)	MAPK10 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	MAPK10 gRNA (BRDN0001146535)	MAPK10 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	MAPK10 gRNA (BRDN0001148098)	MAPK10 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	CAMK1G gRNA (BRDN0001148530)	CAMK1G (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	CAMK1G gRNA (BRDN0001147776)	CAMK1G (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	CAMK1G gRNA (BRDN0001146460)	CAMK1G (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	MASTL gRNA (BRDN0001148082)	MASTL (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	MASTL gRNA (BRDN0001149040)	MASTL (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	MASTL gRNA (BRDN0001148591)	MASTL (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	ROS1 gRNA (BRDN0001149251)	ROS1 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	ROS1 gRNA (BRDN0001149105)	ROS1 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	ROS1 gRNA (BRDN0001147358)	ROS1 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	TLK1 gRNA (BRDN0001145691)	TLK1 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	TLK1 gRNA (BRDN0001147425)	TLK1 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	TLK1 gRNA (BRDN0001147793)	TLK1 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	TLK1 gRNA (BRDN0001146841)	TLK1 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	STK26 gRNA (BRDN0001149000)	STK26 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	STK26 gRNA (BRDN0001146165)	STK26 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	STK26 gRNA (BRDN0001149381)	STK26 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	STK26 gRNA (BRDN0001148628)	STK26 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	PRKCD gRNA (BRDN0001145526)	PRKCD (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	PRKCD gRNA (BRDN0001147700)	PRKCD (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	PRKCD gRNA (BRDN0001147061)	PRKCD (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	MAP3K10 gRNA (BRDN0001148030)	MAP3K10 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	MAP3K10 gRNA (BRDN0001145582)	MAP3K10 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	MAP3K10 gRNA (BRDN0001146111)	MAP3K10 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	ULK4 gRNA (BRDN0001148283)	ULK4 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	ULK4 gRNA (BRDN0001145110)	ULK4 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	ULK4 gRNA (BRDN0001145330)	ULK4 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	ULK4 gRNA (BRDN0001147343)	ULK4 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	MAP3K6 gRNA (BRDN0001162255)	MAP3K6 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	MAP3K6 gRNA (BRDN0001144737)	MAP3K6 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	MAP3K6 gRNA (BRDN0001146318)	MAP3K6 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	MAP3K6 gRNA (BRDN0001162229)	MAP3K6 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	MAP3K3 gRNA (BRDN0001148097)	MAP3K3 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	MAP3K3 gRNA (BRDN0001149355)	MAP3K3 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	MAP3K3 gRNA (BRDN0001147001)	MAP3K3 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	DAPK2 gRNA (BRDN0001145554)	DAPK2 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	DAPK2 gRNA (BRDN0001149451)	DAPK2 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	DAPK2 gRNA (BRDN0001149391)	DAPK2 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	DAPK2 gRNA (BRDN0001144830)	DAPK2 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	LATS1 gRNA (BRDN0001148404)	LATS1 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	LATS1 gRNA (BRDN0001146581)	LATS1 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	LATS1 gRNA (BRDN0001146897)	LATS1 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	LATS1 gRNA (BRDN0001147815)	LATS1 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	STK19 gRNA (BRDN0001145122)	STK19 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	STK19 gRNA (BRDN0001147911)	STK19 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	STK19 gRNA (BRDN0001148415)	STK19 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	STK19 gRNA (BRDN0001144764)	STK19 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	GUCY2D gRNA (BRDN0001146790)	GUCY2D (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	GUCY2D gRNA (BRDN0001146190)	GUCY2D (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	GUCY2D gRNA (BRDN0001147902)	GUCY2D (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	GUCY2D gRNA (BRDN0001145533)	GUCY2D (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	ANKK1 gRNA (BRDN0001149290)	ANKK1 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	ANKK1 gRNA (BRDN0001149403)	ANKK1 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	ANKK1 gRNA (BRDN0001146770)	ANKK1 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	ANKK1 gRNA (BRDN0001149535)	ANKK1 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	MAP2K6 gRNA (BRDN0001145878)	MAP2K6 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	MAP2K6 gRNA (BRDN0001145312)	MAP2K6 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	MAP2K6 gRNA (BRDN0001147036)	MAP2K6 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	ROR1 gRNA (BRDN0001147493)	ROR1 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	ROR1 gRNA (BRDN0001162245)	ROR1 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	ROR1 gRNA (BRDN0001162233)	ROR1 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	ROR1 gRNA (BRDN0001145812)	ROR1 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	LIMK1 gRNA (BRDN0001144875)	LIMK1 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	LIMK1 gRNA (BRDN0001144894)	LIMK1 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	LIMK1 gRNA (BRDN0001148029)	LIMK1 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	LIMK1 gRNA (BRDN0001147777)	LIMK1 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	SCYL2 gRNA (BRDN0001145947)	SCYL2 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	SCYL2 gRNA (BRDN0001148262)	SCYL2 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	SCYL2 gRNA (BRDN0001146013)	SCYL2 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	STRADA gRNA (BRDN0001145789)	STRADA (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	STRADA gRNA (BRDN0001148926)	STRADA (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	STRADA gRNA (BRDN0001145392)	STRADA (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	STRADA gRNA (BRDN0001149192)	STRADA (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	RPS6KB2 gRNA (BRDN0001146372)	RPS6KB2 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	RPS6KB2 gRNA (BRDN0001146023)	RPS6KB2 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	RPS6KB2 gRNA (BRDN0001146316)	RPS6KB2 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	MET gRNA (BRDN0001145767)	MET (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	MET gRNA (BRDN0001147560)	MET (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	MET gRNA (BRDN0001147542)	MET (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	MET gRNA (BRDN0001147257)	MET (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	SCYL1 gRNA (BRDN0001148650)	SCYL1 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	SCYL1 gRNA (BRDN0001146995)	SCYL1 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	SCYL1 gRNA (BRDN0001144725)	SCYL1 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	FGFR1 gRNA (BRDN0001145494)	FGFR1 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	FGFR1 gRNA (BRDN0001146877)	FGFR1 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	FGFR1 gRNA (BRDN0001146517)	FGFR1 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	STK39 gRNA (BRDN0001146271)	STK39 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	STK39 gRNA (BRDN0001145870)	STK39 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	STK39 gRNA (BRDN0001147207)	STK39 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	STK39 gRNA (BRDN0001148139)	STK39 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	STK4 gRNA (BRDN0001149002)	STK4 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	STK4 gRNA (BRDN0001147987)	STK4 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	STK4 gRNA (BRDN0001148451)	STK4 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	CDK7 gRNA (BRDN0001162216)	CDK7 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	CDK7 gRNA (BRDN0001146106)	CDK7 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	CDK7 gRNA (BRDN0001162589)	CDK7 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	CDK7 gRNA (BRDN0001147450)	CDK7 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	ALPK1 gRNA (BRDN0001146035)	ALPK1 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	ALPK1 gRNA (BRDN0001146195)	ALPK1 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	ALPK1 gRNA (BRDN0001145294)	ALPK1 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	ALPK1 gRNA (BRDN0001146875)	ALPK1 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	ADCK1 gRNA (BRDN0001147030)	ADCK1 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	ADCK1 gRNA (BRDN0001146894)	ADCK1 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	ADCK1 gRNA (BRDN0001149367)	ADCK1 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	IKBKB gRNA (BRDN0001145640)	IKBKB (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	IKBKB gRNA (BRDN0001145616)	IKBKB (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	IKBKB gRNA (BRDN0001145579)	IKBKB (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	IKBKB gRNA (BRDN0001148638)	IKBKB (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	BRD3 gRNA (BRDN0001146442)	BRD3 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	BRD3 gRNA (BRDN0001149039)	BRD3 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	BRD3 gRNA (BRDN0001147217)	BRD3 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	BRD3 gRNA (BRDN0001148860)	BRD3 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	EPHA10 gRNA (BRDN0001146992)	EPHA10 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	EPHA10 gRNA (BRDN0001144827)	EPHA10 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	EPHA10 gRNA (BRDN0001146813)	EPHA10 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	EPHA10 gRNA (BRDN0001146043)	EPHA10 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	MAP3K15 gRNA (BRDN0001149244)	MAP3K15 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	MAP3K15 gRNA (BRDN0001147819)	MAP3K15 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	MAP3K15 gRNA (BRDN0001146305)	MAP3K15 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	MAST3 gRNA (BRDN0001145286)	MAST3 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	MAST3 gRNA (BRDN0001147549)	MAST3 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	MAST3 gRNA (BRDN0001147113)	MAST3 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	CDKL4 gRNA (BRDN0001146173)	CDKL4 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	CDKL4 gRNA (BRDN0001149006)	CDKL4 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	CDKL4 gRNA (BRDN0001149476)	CDKL4 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	CDKL4 gRNA (BRDN0001147188)	CDKL4 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	TRPM7 gRNA (BRDN0001148025)	TRPM7 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	TRPM7 gRNA (BRDN0001146152)	TRPM7 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	TRPM7 gRNA (BRDN0001147821)	TRPM7 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	TRPM7 gRNA (BRDN0001145424)	TRPM7 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	OBSCN gRNA (BRDN0001145479)	OBSCN (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	OBSCN gRNA (BRDN0001147726)	OBSCN (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	OBSCN gRNA (BRDN0001146767)	OBSCN (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	NEK5 gRNA (BRDN0001146851)	NEK5 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	NEK5 gRNA (BRDN0001144996)	NEK5 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	NEK5 gRNA (BRDN0001148329)	NEK5 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	NEK5 gRNA (BRDN0001148037)	NEK5 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	MAST1 gRNA (BRDN0001149113)	MAST1 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	MAST1 gRNA (BRDN0001145937)	MAST1 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	MAST1 gRNA (BRDN0001148834)	MAST1 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	MAST1 gRNA (BRDN0001148956)	MAST1 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	NEK1 gRNA (BRDN0001146251)	NEK1 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	NEK1 gRNA (BRDN0001149235)	NEK1 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	NEK1 gRNA (BRDN0001148922)	NEK1 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	NEK1 gRNA (BRDN0001145167)	NEK1 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	MYT1 gRNA (BRDN0001147389)	MYT1 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	MYT1 gRNA (BRDN0001149058)	MYT1 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	MYT1 gRNA (BRDN0001148099)	MYT1 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	BAZ1B gRNA (BRDN0001144929)	BAZ1B (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	BAZ1B gRNA (BRDN0001145459)	BAZ1B (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	BAZ1B gRNA (BRDN0001147144)	BAZ1B (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	IP6K1 gRNA (BRDN0001147847)	IP6K1 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	IP6K1 gRNA (BRDN0001149166)	IP6K1 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	IP6K1 gRNA (BRDN0001148869)	IP6K1 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	IP6K1 gRNA (BRDN0001146092)	IP6K1 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	TRIM28 gRNA (BRDN0001162487)	TRIM28 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	TRIM28 gRNA (BRDN0001162440)	TRIM28 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	TRIM28 gRNA (BRDN0001145369)	TRIM28 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	TRIM28 gRNA (BRDN0001146162)	TRIM28 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	CLP1 gRNA (BRDN0001146849)	CLP1 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	CLP1 gRNA (BRDN0001148453)	CLP1 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	CLP1 gRNA (BRDN0001145024)	CLP1 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	CLP1 gRNA (BRDN0001146499)	CLP1 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	OSR1 gRNA (BRDN0001146927)	OSR1 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	OSR1 gRNA (BRDN0001145611)	OSR1 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	OSR1 gRNA (BRDN0001145425)	OSR1 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	PRKAR2B gRNA (BRDN0001147301)	PRKAR2B (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	PRKAR2B gRNA (BRDN0001147315)	PRKAR2B (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	PRKAR2B gRNA (BRDN0001147707)	PRKAR2B (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	AGK gRNA (BRDN0001147162)	AGK (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	AGK gRNA (BRDN0001146005)	AGK (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	AGK gRNA (BRDN0001146482)	AGK (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	DLG3 gRNA (BRDN0001147306)	DLG3 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	DLG3 gRNA (BRDN0001145728)	DLG3 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	DLG3 gRNA (BRDN0001149280)	DLG3 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	ULK3 gRNA (BRDN0001145678)	ULK3 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	ULK3 gRNA (BRDN0001145965)	ULK3 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	ULK3 gRNA (BRDN0001162314)	ULK3 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	ULK3 gRNA (BRDN0001162437)	ULK3 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	PIK3CD gRNA (BRDN0001146527)	PIK3CD (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	PIK3CD gRNA (BRDN0001146545)	PIK3CD (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	ETNK2 gRNA (BRDN0001149182)	ETNK2 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	ETNK2 gRNA (BRDN0001149328)	ETNK2 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	ETNK2 gRNA (BRDN0001147513)	ETNK2 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	ETNK2 gRNA (BRDN0001149306)	ETNK2 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	NME4 gRNA (BRDN0001147482)	NME4 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	NME4 gRNA (BRDN0001149238)	NME4 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	NME4 gRNA (BRDN0001145289)	NME4 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	LMTK2 gRNA (BRDN0001145198)	LMTK2 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	LMTK2 gRNA (BRDN0001145764)	LMTK2 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	LMTK2 gRNA (BRDN0001148094)	LMTK2 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	CDK4 gRNA (BRDN0001145730)	CDK4 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	CDK4 gRNA (BRDN0001147537)	CDK4 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	CDK4 gRNA (BRDN0001147572)	CDK4 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	NTRK3 gRNA (BRDN0001145045)	NTRK3 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	NTRK3 gRNA (BRDN0001145325)	NTRK3 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	NTRK3 gRNA (BRDN0001147858)	NTRK3 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	FGFR2 gRNA (BRDN0001148071)	FGFR2 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	FGFR2 gRNA (BRDN0001147365)	FGFR2 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	FGFR2 gRNA (BRDN0001149360)	FGFR2 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	RAB11FIP5 gRNA (BRDN0001148210)	RAB11FIP5 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	RAB11FIP5 gRNA (BRDN0001147136)	RAB11FIP5 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	RAB11FIP5 gRNA (BRDN0001148953)	RAB11FIP5 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	CSNK1A1 gRNA (BRDN0001149315)	CSNK1A1 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	CSNK1A1 gRNA (BRDN0001145680)	CSNK1A1 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	CSNK1A1 gRNA (BRDN0001145896)	CSNK1A1 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	PIK3CB gRNA (BRDN0001147701)	PIK3CB (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	PIK3CB gRNA (BRDN0001145915)	PIK3CB (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	PIK3CB gRNA (BRDN0001145643)	PIK3CB (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	PIK3CB gRNA (BRDN0001147768)	PIK3CB (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	ERBB4 gRNA (BRDN0001162372)	ERBB4 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	ERBB4 gRNA (BRDN0001149467)	ERBB4 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	ERBB4 gRNA (BRDN0001146197)	ERBB4 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	ERBB4 gRNA (BRDN0001162324)	ERBB4 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	PRPS1 gRNA (BRDN0001147960)	PRPS1 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	PRPS1 gRNA (BRDN0001147441)	PRPS1 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	PRPS1 gRNA (BRDN0001146971)	PRPS1 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	PRPS1 gRNA (BRDN0001147353)	PRPS1 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	PASK gRNA (BRDN0001145672)	PASK (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	PASK gRNA (BRDN0001149035)	PASK (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	PASK gRNA (BRDN0001148005)	PASK (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	SGK2 gRNA (BRDN0001145816)	SGK2 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	SGK2 gRNA (BRDN0001148976)	SGK2 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	SGK2 gRNA (BRDN0001149504)	SGK2 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	SGK2 gRNA (BRDN0001147898)	SGK2 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	MAP2K1 gRNA (BRDN0001145794)	MAP2K1 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	MAP2K1 gRNA (BRDN0001147078)	MAP2K1 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	MAP2K1 gRNA (BRDN0001147218)	MAP2K1 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	MAP2K1 gRNA (BRDN0001146127)	MAP2K1 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	NEK2 gRNA (BRDN0001146193)	NEK2 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	NEK2 gRNA (BRDN0001145653)	NEK2 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	NEK2 gRNA (BRDN0001145920)	NEK2 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	AKT3 gRNA (BRDN0001146868)	AKT3 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	AKT3 gRNA (BRDN0001144935)	AKT3 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	AKT3 gRNA (BRDN0001145071)	AKT3 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	AKT3 gRNA (BRDN0001147841)	AKT3 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	NME6 gRNA (BRDN0001148253)	NME6 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	NME6 gRNA (BRDN0001145496)	NME6 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	NME6 gRNA (BRDN0001146177)	NME6 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	MYO3A gRNA (BRDN0001145770)	MYO3A (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	MYO3A gRNA (BRDN0001148088)	MYO3A (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	MYO3A gRNA (BRDN0001146256)	MYO3A (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	TYRO3 gRNA (BRDN0001147880)	TYRO3 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	TYRO3 gRNA (BRDN0001146050)	TYRO3 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	TYRO3 gRNA (BRDN0001147403)	TYRO3 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	CLK1 gRNA (BRDN0001146512)	CLK1 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	CLK1 gRNA (BRDN0001147209)	CLK1 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	CLK1 gRNA (BRDN0001146454)	CLK1 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	CLK1 gRNA (BRDN0001147408)	CLK1 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	PLK4 gRNA (BRDN0001146388)	PLK4 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	PLK4 gRNA (BRDN0001147289)	PLK4 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	PLK4 gRNA (BRDN0001147082)	PLK4 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	CAMK2G gRNA (BRDN0001148688)	CAMK2G (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	CAMK2G gRNA (BRDN0001148569)	CAMK2G (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	CAMK2G gRNA (BRDN0001147974)	CAMK2G (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	FRK gRNA (BRDN0001144749)	FRK (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	FRK gRNA (BRDN0001149083)	FRK (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	FRK gRNA (BRDN0001146590)	FRK (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	FRK gRNA (BRDN0001148904)	FRK (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	TSSK6 gRNA (BRDN0001147567)	TSSK6 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	TSSK6 gRNA (BRDN0001148928)	TSSK6 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	TSSK6 gRNA (BRDN0001146415)	TSSK6 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	EPHA6 gRNA (BRDN0001146458)	EPHA6 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	EPHA6 gRNA (BRDN0001145443)	EPHA6 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	EPHA6 gRNA (BRDN0001146068)	EPHA6 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	MAPK13 gRNA (BRDN0001149329)	MAPK13 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	MAPK13 gRNA (BRDN0001145247)	MAPK13 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	MAPK13 gRNA (BRDN0001145708)	MAPK13 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	PRKAA1 gRNA (BRDN0001146526)	PRKAA1 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	PRKAA1 gRNA (BRDN0001147273)	PRKAA1 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	PRKAA1 gRNA (BRDN0001149089)	PRKAA1 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	NEK6 gRNA (BRDN0001147000)	NEK6 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	NEK6 gRNA (BRDN0001144960)	NEK6 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	NEK6 gRNA (BRDN0001148151)	NEK6 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	MINK1 gRNA (BRDN0001144839)	MINK1 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	MINK1 gRNA (BRDN0001146260)	MINK1 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	MINK1 gRNA (BRDN0001145021)	MINK1 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	MINK1 gRNA (BRDN0001147795)	MINK1 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	MAP4K4 gRNA (BRDN0001148610)	MAP4K4 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	MAP4K4 gRNA (BRDN0001162594)	MAP4K4 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	MAP4K4 gRNA (BRDN0001146036)	MAP4K4 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	MAP4K4 gRNA (BRDN0001162353)	MAP4K4 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	CDKL1 gRNA (BRDN0001148660)	CDKL1 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	CDKL1 gRNA (BRDN0001148269)	CDKL1 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	CDKL1 gRNA (BRDN0001147644)	CDKL1 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	CDKL1 gRNA (BRDN0001148261)	CDKL1 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	GRK5 gRNA (BRDN0001149126)	GRK5 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	GRK5 gRNA (BRDN0001149298)	GRK5 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	GRK5 gRNA (BRDN0001149127)	GRK5 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	GRK5 gRNA (BRDN0001148840)	GRK5 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	PKN1 gRNA (BRDN0001149250)	PKN1 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	PKN1 gRNA (BRDN0001162227)	PKN1 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	PKN1 gRNA (BRDN0001148556)	PKN1 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	PKN1 gRNA (BRDN0001162558)	PKN1 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	SRM gRNA (BRDN0001147064)	SRM (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	SRM gRNA (BRDN0001149020)	SRM (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	SRM gRNA (BRDN0001147759)	SRM (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	RP2 gRNA (BRDN0001148096)	RP2 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	RP2 gRNA (BRDN0001145650)	RP2 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	RP2 gRNA (BRDN0001144983)	RP2 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	RP2 gRNA (BRDN0001145706)	RP2 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	PCK1 gRNA (BRDN0001146891)	PCK1 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	PCK1 gRNA (BRDN0001148468)	PCK1 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	PCK1 gRNA (BRDN0001145528)	PCK1 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	PCK1 gRNA (BRDN0001146817)	PCK1 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	TGFBR3 gRNA (BRDN0001145124)	TGFBR3 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	TGFBR3 gRNA (BRDN0001146153)	TGFBR3 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	TGFBR3 gRNA (BRDN0001144841)	TGFBR3 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	PANK3 gRNA (BRDN0001148432)	PANK3 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	PANK3 gRNA (BRDN0001148598)	PANK3 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	PANK3 gRNA (BRDN0001144785)	PANK3 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	DSTYK gRNA (BRDN0001147753)	DSTYK (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	DSTYK gRNA (BRDN0001148274)	DSTYK (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	DSTYK gRNA (BRDN0001146496)	DSTYK (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	PRKRA gRNA (BRDN0001146443)	PRKRA (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	PRKRA gRNA (BRDN0001147957)	PRKRA (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	PRKRA gRNA (BRDN0001146546)	PRKRA (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	FASTKD2 gRNA (BRDN0001145464)	FASTKD2 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	FASTKD2 gRNA (BRDN0001147886)	FASTKD2 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	FASTKD2 gRNA (BRDN0001148171)	FASTKD2 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	FASTKD2 gRNA (BRDN0001148576)	FASTKD2 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	PPIP5K1 gRNA (BRDN0001146565)	PPIP5K1 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	PPIP5K1 gRNA (BRDN0001146986)	PPIP5K1 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	PPIP5K1 gRNA (BRDN0001148626)	PPIP5K1 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	HK2 gRNA (BRDN0001162236)	HK2 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	HK2 gRNA (BRDN0001162212)	HK2 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	HK2 gRNA (BRDN0001162355)	HK2 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	HK2 gRNA (BRDN0001149267)	HK2 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	NRK gRNA (BRDN0001146231)	NRK (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	NRK gRNA (BRDN0001147558)	NRK (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	NRK gRNA (BRDN0001144922)	NRK (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	PAPSS2 gRNA (BRDN0001145342)	PAPSS2 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	PAPSS2 gRNA (BRDN0001147912)	PAPSS2 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	PAPSS2 gRNA (BRDN0001146774)	PAPSS2 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	PKD2 gRNA (BRDN0001148691)	PKD2 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	PKD2 gRNA (BRDN0001144799)	PKD2 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	PKD2 gRNA (BRDN0001146258)	PKD2 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	NAGK gRNA (BRDN0001148128)	NAGK (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	NAGK gRNA (BRDN0001148511)	NAGK (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	NAGK gRNA (BRDN0001146503)	NAGK (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	NAGK gRNA (BRDN0001146760)	NAGK (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	GK gRNA (BRDN0001148115)	GK (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	GK gRNA (BRDN0001145307)	GK (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	GK gRNA (BRDN0001146936)	GK (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	GK gRNA (BRDN0001146383)	GK (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	CERK gRNA (BRDN0001148273)	CERK (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	CERK gRNA (BRDN0001145454)	CERK (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	CERK gRNA (BRDN0001146528)	CERK (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	PIK3CG gRNA (BRDN0001147429)	PIK3CG (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	PIK3CG gRNA (BRDN0001149186)	PIK3CG (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	PIK3CG gRNA (BRDN0001149132)	PIK3CG (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	N4BP2 gRNA (BRDN0001146240)	N4BP2 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	N4BP2 gRNA (BRDN0001146515)	N4BP2 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	N4BP2 gRNA (BRDN0001146056)	N4BP2 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	PPIP5K2 gRNA (BRDN0001148509)	PPIP5K2 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	PPIP5K2 gRNA (BRDN0001146353)	PPIP5K2 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	PPIP5K2 gRNA (BRDN0001148255)	PPIP5K2 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	PIK3R3 gRNA (BRDN0001145523)	PIK3R3 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	PIK3R3 gRNA (BRDN0001147854)	PIK3R3 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	PIK3R3 gRNA (BRDN0001146614)	PIK3R3 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	UCKL1 gRNA (BRDN0001146668)	UCKL1 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	UCKL1 gRNA (BRDN0001162217)	UCKL1 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	UCKL1 gRNA (BRDN0001147183)	UCKL1 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	UCKL1 gRNA (BRDN0001162519)	UCKL1 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	ROCK2 gRNA (BRDN0001147800)	ROCK2 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	ROCK2 gRNA (BRDN0001146481)	ROCK2 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	ROCK2 gRNA (BRDN0001145177)	ROCK2 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	EPHB4 gRNA (BRDN0001144879)	EPHB4 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	EPHB4 gRNA (BRDN0001148436)	EPHB4 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	EPHB4 gRNA (BRDN0001145313)	EPHB4 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	PHKG1 gRNA (BRDN0001147570)	PHKG1 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	PHKG1 gRNA (BRDN0001144981)	PHKG1 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	PHKG1 gRNA (BRDN0001147981)	PHKG1 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	TXK gRNA (BRDN0001145857)	TXK (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	TXK gRNA (BRDN0001145866)	TXK (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	TXK gRNA (BRDN0001146212)	TXK (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	TBK1 gRNA (BRDN0001145663)	TBK1 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	TBK1 gRNA (BRDN0001148392)	TBK1 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	TBK1 gRNA (BRDN0001148607)	TBK1 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	MAP3K11 gRNA (BRDN0001146472)	MAP3K11 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	MAP3K11 gRNA (BRDN0001145849)	MAP3K11 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	MAP3K11 gRNA (BRDN0001144833)	MAP3K11 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	EPHA2 gRNA (BRDN0001146397)	EPHA2 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	EPHA2 gRNA (BRDN0001147157)	EPHA2 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	EPHA2 gRNA (BRDN0001147488)	EPHA2 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	GSK3A gRNA (BRDN0001148594)	GSK3A (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	GSK3A gRNA (BRDN0001148086)	GSK3A (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	GSK3A gRNA (BRDN0001147712)	GSK3A (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	STK32B gRNA (BRDN0001146403)	STK32B (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	STK32B gRNA (BRDN0001145394)	STK32B (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	STK32B gRNA (BRDN0001144884)	STK32B (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	ERBB2 gRNA (BRDN0001146057)	ERBB2 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	ERBB2 gRNA (BRDN0001148673)	ERBB2 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	ERBB2 gRNA (BRDN0001148320)	ERBB2 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	MAP2K3 gRNA (BRDN0001147291)	MAP2K3 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	MAP2K3 gRNA (BRDN0001146677)	MAP2K3 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	MAP2K3 gRNA (BRDN0001147239)	MAP2K3 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	PIM2 gRNA (BRDN0001148470)	PIM2 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	PIM2 gRNA (BRDN0001149335)	PIM2 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	PIM2 gRNA (BRDN0001149489)	PIM2 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	PIM2 gRNA (BRDN0001147820)	PIM2 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	PTK2B gRNA (BRDN0001148546)	PTK2B (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	PTK2B gRNA (BRDN0001148298)	PTK2B (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	PTK2B gRNA (BRDN0001147875)	PTK2B (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	BMPR2 gRNA (BRDN0001146229)	BMPR2 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	BMPR2 gRNA (BRDN0001144950)	BMPR2 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	BMPR2 gRNA (BRDN0001144883)	BMPR2 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	JAK1 gRNA (BRDN0001145887)	JAK1 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	JAK1 gRNA (BRDN0001144778)	JAK1 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	JAK1 gRNA (BRDN0001149081)	JAK1 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	JAK1 gRNA (BRDN0001149025)	JAK1 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	MAPK6 gRNA (BRDN0001148999)	MAPK6 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	MAPK6 gRNA (BRDN0001146144)	MAPK6 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	MAPK6 gRNA (BRDN0001149260)	MAPK6 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	MAPK6 gRNA (BRDN0001146542)	MAPK6 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	PRKCI gRNA (BRDN0001146507)	PRKCI (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	PRKCI gRNA (BRDN0001148989)	PRKCI (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	PRKCI gRNA (BRDN0001162413)	PRKCI (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	PRKCI gRNA (BRDN0001162218)	PRKCI (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	MAP4K3 gRNA (BRDN0001145375)	MAP4K3 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	MAP4K3 gRNA (BRDN0001145020)	MAP4K3 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	MAP4K3 gRNA (BRDN0001147653)	MAP4K3 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	ERN1 gRNA (BRDN0001162304)	ERN1 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	ERN1 gRNA (BRDN0001146341)	ERN1 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	ERN1 gRNA (BRDN0001162231)	ERN1 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	ERN1 gRNA (BRDN0001149471)	ERN1 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	LCK gRNA (BRDN0001145572)	LCK (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	LCK gRNA (BRDN0001145503)	LCK (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	LCK gRNA (BRDN0001145758)	LCK (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	PIK3C2A gRNA (BRDN0001149181)	PIK3C2A (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	PIK3C2A gRNA (BRDN0001148547)	PIK3C2A (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	PIK3C2A gRNA (BRDN0001145757)	PIK3C2A (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	PIK3C2A gRNA (BRDN0001149070)	PIK3C2A (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	IRAK1 gRNA (BRDN0001145800)	IRAK1 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	IRAK1 gRNA (BRDN0001147615)	IRAK1 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	IRAK1 gRNA (BRDN0001145661)	IRAK1 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	IRAK1 gRNA (BRDN0001146412)	IRAK1 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	TK1 gRNA (BRDN0001147866)	TK1 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	TK1 gRNA (BRDN0001147625)	TK1 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	TK1 gRNA (BRDN0001145069)	TK1 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	KSR2 gRNA (BRDN0001147424)	KSR2 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	KSR2 gRNA (BRDN0001148172)	KSR2 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	KSR2 gRNA (BRDN0001145237)	KSR2 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	PRPF4B gRNA (BRDN0001146164)	PRPF4B (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	PRPF4B gRNA (BRDN0001148328)	PRPF4B (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	PRPF4B gRNA (BRDN0001146471)	PRPF4B (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	PRPF4B gRNA (BRDN0001146215)	PRPF4B (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	TESK2 gRNA (BRDN0001146547)	TESK2 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	TESK2 gRNA (BRDN0001149031)	TESK2 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	TESK2 gRNA (BRDN0001147688)	TESK2 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	TESK2 gRNA (BRDN0001149439)	TESK2 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	PDK4 gRNA (BRDN0001145641)	PDK4 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	PDK4 gRNA (BRDN0001147610)	PDK4 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	PDK4 gRNA (BRDN0001148073)	PDK4 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	PDK4 gRNA (BRDN0001146844)	PDK4 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	PDIK1L gRNA (BRDN0001148388)	PDIK1L (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	PDIK1L gRNA (BRDN0001145465)	PDIK1L (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	PDIK1L gRNA (BRDN0001145634)	PDIK1L (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	PDIK1L gRNA (BRDN0001147645)	PDIK1L (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	MERTK gRNA (BRDN0001145505)	MERTK (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	MERTK gRNA (BRDN0001144964)	MERTK (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	MERTK gRNA (BRDN0001146205)	MERTK (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	MAPKAPK5 gRNA (BRDN0001146604)	MAPKAPK5 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	MAPKAPK5 gRNA (BRDN0001147153)	MAPKAPK5 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	MAPKAPK5 gRNA (BRDN0001145783)	MAPKAPK5 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	RYK gRNA (BRDN0001149332)	RYK (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	RYK gRNA (BRDN0001146411)	RYK (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	RYK gRNA (BRDN0001146690)	RYK (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	NRBP2 gRNA (BRDN0001147825)	NRBP2 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	NRBP2 gRNA (BRDN0001147090)	NRBP2 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	NRBP2 gRNA (BRDN0001145030)	NRBP2 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	NRBP2 gRNA (BRDN0001146685)	NRBP2 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	PLK1 gRNA (BRDN0001145759)	PLK1 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	PLK1 gRNA (BRDN0001144743)	PLK1 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	PLK1 gRNA (BRDN0001144995)	PLK1 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	PLK1 gRNA (BRDN0001147678)	PLK1 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	PFKFB3 gRNA (BRDN0001147151)	PFKFB3 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	PFKFB3 gRNA (BRDN0001146230)	PFKFB3 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	PFKFB3 gRNA (BRDN0001147212)	PFKFB3 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	PAK1 gRNA (BRDN0001145958)	PAK1 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	PAK1 gRNA (BRDN0001147867)	PAK1 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	PAK1 gRNA (BRDN0001145359)	PAK1 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	NTRK2 gRNA (BRDN0001146241)	NTRK2 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	NTRK2 gRNA (BRDN0001148065)	NTRK2 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	NTRK2 gRNA (BRDN0001146295)	NTRK2 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	GAK gRNA (BRDN0001146687)	GAK (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	GAK gRNA (BRDN0001146263)	GAK (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	GAK gRNA (BRDN0001148048)	GAK (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	STK32A gRNA (BRDN0001146573)	STK32A (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	STK32A gRNA (BRDN0001145029)	STK32A (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	STK32A gRNA (BRDN0001145147)	STK32A (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	TNK1 gRNA (BRDN0001149443)	TNK1 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	TNK1 gRNA (BRDN0001149201)	TNK1 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	TNK1 gRNA (BRDN0001146081)	TNK1 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	BRAF gRNA (BRDN0001145345)	BRAF (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	BRAF gRNA (BRDN0001146266)	BRAF (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	BRAF gRNA (BRDN0001146755)	BRAF (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	BRAF gRNA (BRDN0001147638)	BRAF (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	CDK15 gRNA (BRDN0001145059)	CDK15 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	CDK15 gRNA (BRDN0001148122)	CDK15 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	CDK15 gRNA (BRDN0001146647)	CDK15 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	CDK15 gRNA (BRDN0001144734)	CDK15 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	CHEK2 gRNA (BRDN0001145610)	CHEK2 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	CHEK2 gRNA (BRDN0001146385)	CHEK2 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	CHEK2 gRNA (BRDN0001145828)	CHEK2 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	PTK6 gRNA (BRDN0001149364)	PTK6 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	PTK6 gRNA (BRDN0001162471)	PTK6 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	PTK6 gRNA (BRDN0001145574)	PTK6 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	PTK6 gRNA (BRDN0001162535)	PTK6 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	NME7 gRNA (BRDN0001146186)	NME7 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	NME7 gRNA (BRDN0001145354)	NME7 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	NME7 gRNA (BRDN0001148617)	NME7 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	NME7 gRNA (BRDN0001146300)	NME7 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	GRK7 gRNA (BRDN0001145046)	GRK7 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	GRK7 gRNA (BRDN0001147785)	GRK7 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	GRK7 gRNA (BRDN0001148138)	GRK7 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	CDK19 gRNA (BRDN0001149215)	CDK19 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	CDK19 gRNA (BRDN0001148843)	CDK19 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	CDK19 gRNA (BRDN0001145270)	CDK19 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	CDK19 gRNA (BRDN0001148326)	CDK19 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	FES gRNA (BRDN0001148684)	FES (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	FES gRNA (BRDN0001147775)	FES (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	FES gRNA (BRDN0001147481)	FES (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	FES gRNA (BRDN0001147807)	FES (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	MAP3K4 gRNA (BRDN0001148639)	MAP3K4 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	MAP3K4 gRNA (BRDN0001148603)	MAP3K4 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	MAP3K4 gRNA (BRDN0001148693)	MAP3K4 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	MAP3K4 gRNA (BRDN0001146051)	MAP3K4 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	CAMKK1 gRNA (BRDN0001145638)	CAMKK1 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	CAMKK1 gRNA (BRDN0001148386)	CAMKK1 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	CAMKK1 gRNA (BRDN0001147371)	CAMKK1 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
	DAPK1 gRNA (BRDN0001146680)	DAPK1 (Homo sapiens)	Mammalian Expression, Lentiviral, CRISPR	Root	Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.