| Peft-3::cas9-SV40_NLS::tbb-2 3'UTR | codon optimized Cas9_SV40 NLS with intron (Synthetic) | eef-1A.1 (eft-3) |
Calarco
| Heritable genome editing in C. elegans via a CRISPR-Cas9 system.
Nat Methods. 2013 Jun 30. doi: 10.1038/nmeth.2532.
|
pUC57
|
| pDD162 (Peft-3::Cas9 + Empty sgRNA) | Cas9 (Synthetic), Empty sgRNA (Other) | eef-1A.1 (eft-3) , U6 |
Goldstein
| Engineering the Caenorhabditis elegans genome using Cas9-triggered homologous recombination.
Nat Methods. 2013 Sep 1. doi: 10.1038/nmeth.2641.
|
Cas9 + sgRNA plasmid that can be modified to cleave any Cas9 target site in the C. elegans genome.
pCFJ601
|
| SP6-hCas9-Ce-mRNA | hCas9 with C. elegans 5' and 3' UTRs (Homo sapiens) | SP6 |
Sternberg
| Transgene-Free Genome Editing in Caenorhabditis elegans Using CRISPR-Cas.
Genetics. 2013 Aug 26.
|
In vitro transcription of a mRNA encoding humanized Cas9 nuclease, with 3' UTRs suitable for germline expression in C. elegans, for doing CRISPR-Cas by RNA injection. Uses SP6 RNA polymerase.
Bluescript
|
| pIK86 | Cas9 (Synthetic) | T7 |
Katic
| Targeted Heritable Mutation and Gene Conversion by Cas9-CRISPR in Caenorhabditis elegans.
Genetics. 2013 Aug 26.
|
C. elegans codon-optimized Cas9 with 2xSV40 NLS sequences. It has a T7 promoter for in vitro transcription, germline-compatible 5'UTR, tbb-2 3'UTR and a polyA sequence.
pUC57
|
| pMB62 | Cas9 (Synthetic) | eef-1A.1 (eft-3) |
Boxem
| CRISPR/Cas9-Targeted Mutagenesis in Caenorhabditis elegans.
Genetics. 2013 Aug 26.
|
Expresses C. elegans optimized Cas9::mEGFP from the eef-1A.1 (eft-3) promoter
pBluescript SK+
|
| pMB63 | Cas9 (Synthetic) | eef-1A.1 (eft-3) |
Boxem
| CRISPR/Cas9-Targeted Mutagenesis in Caenorhabditis elegans.
Genetics. 2013 Aug 26.
|
Expresses C. elegans optimized Cas9 from the eef-1A.1 (eft-3) promoter
pBluescript SK+
|
| pMB66 | Cas9 (Synthetic) | hsp-16.48 |
Boxem
| CRISPR/Cas9-Targeted Mutagenesis in Caenorhabditis elegans.
Genetics. 2013 Aug 26.
|
Expresses C. elegans optimized Cas9::mEGFP from the hsp-16.48 promoter
pBluescript SK+
|
| pMB67 | Cas9 (Synthetic) | hsp-16.48 |
Boxem
| CRISPR/Cas9-Targeted Mutagenesis in Caenorhabditis elegans.
Genetics. 2013 Aug 26.
|
Expresses C. elegans optimized Cas9 from the hsp-16.48 promoter
pBluescript SK+
|
| peft-3::cas-9::tbb-2 3'UTR | cas9 | eef-1A.1 (eft-3) |
de Bono
| Efficient genome editing in Caenorhabditis elegans by CRISPR-targeted homologous recombination.
Nucleic Acids Res. 2013 Sep 5.
|
this plasmid contains the codon optimized gene encoding Cas9 for C. elegans.
pDEST
|
| pJW1259 | Deletion | |
Ward
| Rapid and Precise Engineering of the Caenorhabditis elegans Genome with Lethal Mutation Co-conversion and Inactivation of NHEJ Repair.
Genetics. 2014 Dec 9. pii: genetics.114.172361.
|
Cas9 plasmid with sgRNA deleted; for use in C. elegans
pJW1219
|
| pME-Cas9 | codon optimized Cas9 (Other) | |
Chen
| Multiplex Conditional Mutagenesis Using Transgenic Expression of Cas9 and sgRNAs.
Genetics. 2015 Apr 8. pii: genetics.115.176917.
|
expresses codon optimized Cas9 in zebrafish
pME-MCS
|
| AP56-5 | sgRNA for APa13 (Caenorhabditis elegans) | U6 |
Seydoux
| Scalable and versatile genome editing using linear DNAs with microhomology to Cas9 Sites in Caenorhabditis elegans.
Genetics. 2014 Dec;198(4):1347-56. doi: 10.1534/genetics.114.170423. Epub 2014 Sep 23.
|
co-expression of Cas9 and a sgRNA targeting 3'end of C.elegans fbf-2 gene
pDD162
|
| AP56-6 | sgRNA for APa13 (Caenorhabditis elegans) | U6 |
Seydoux
| Scalable and versatile genome editing using linear DNAs with microhomology to Cas9 Sites in Caenorhabditis elegans.
Genetics. 2014 Dec;198(4):1347-56. doi: 10.1534/genetics.114.170423. Epub 2014 Sep 23.
|
co-expression of Cas9 and a sgRNA targeting 3'end of C.elegans fbf-2 gene
pDD162
|
| AP56-7 | sgRNA for APa13 (Caenorhabditis elegans) | U6 |
Seydoux
| Scalable and versatile genome editing using linear DNAs with microhomology to Cas9 Sites in Caenorhabditis elegans.
Genetics. 2014 Dec;198(4):1347-56. doi: 10.1534/genetics.114.170423. Epub 2014 Sep 23.
|
co-expression of Cas9 and a sgRNA targeting 3'end of C.elegans fbf-2 gene
pDD162
|
| AP56-8 | sgRNA for APa13 (Caenorhabditis elegans) | U6 |
Seydoux
| Scalable and versatile genome editing using linear DNAs with microhomology to Cas9 Sites in Caenorhabditis elegans.
Genetics. 2014 Dec;198(4):1347-56. doi: 10.1534/genetics.114.170423. Epub 2014 Sep 23.
|
co-expression of Cas9 and a sgRNA targeting 3'end of C.elegans fbf-2 gene
pDD162
|
| AP54-3 | sgRNA for APs12 (Caenorhabditis elegans) | U6 |
Seydoux
| Scalable and versatile genome editing using linear DNAs with microhomology to Cas9 Sites in Caenorhabditis elegans.
Genetics. 2014 Dec;198(4):1347-56. doi: 10.1534/genetics.114.170423. Epub 2014 Sep 23.
|
co-expression of Cas9 and a sgRNA targeting 3'end of C.elegans fbf-2 gene
pDD162
|
| AP54-5 | sgRNA for APs12 (Caenorhabditis elegans) | U6 |
Seydoux
| Scalable and versatile genome editing using linear DNAs with microhomology to Cas9 Sites in Caenorhabditis elegans.
Genetics. 2014 Dec;198(4):1347-56. doi: 10.1534/genetics.114.170423. Epub 2014 Sep 23.
|
co-expression of Cas9 and a sgRNA targeting 3'end of C.elegans fbf-2 gene
pDD162
|
| APq5271 | sgRNA for APa4-2 | U6 |
Seydoux
| Scalable and versatile genome editing using linear DNAs with microhomology to Cas9 Sites in Caenorhabditis elegans.
Genetics. 2014 Dec;198(4):1347-56. doi: 10.1534/genetics.114.170423. Epub 2014 Sep 23.
|
co-expression of Cas9 and a sgRNA targeting K08F4.2 in the middle of the ORF
pDD162
|
| APq5210 | sgRNA for APa4-2 | U6 |
Seydoux
| Scalable and versatile genome editing using linear DNAs with microhomology to Cas9 Sites in Caenorhabditis elegans.
Genetics. 2014 Dec;198(4):1347-56. doi: 10.1534/genetics.114.170423. Epub 2014 Sep 23.
|
co-expression of Cas9 and a sgRNA targeting K08F4.2 in the middle of the ORF
pDD162
|
| AP303-3 | sgRNA for APs1 | U6 |
Seydoux
| Scalable and versatile genome editing using linear DNAs with microhomology to Cas9 Sites in Caenorhabditis elegans.
Genetics. 2014 Dec;198(4):1347-56. doi: 10.1534/genetics.114.170423. Epub 2014 Sep 23.
|
co-expression of Cas9 and a sgRNA targeting 5'end of C.elegans K08F4.2
pDD162
|
| AP303-4 | sgRNA for APs1 | U6 |
Seydoux
| Scalable and versatile genome editing using linear DNAs with microhomology to Cas9 Sites in Caenorhabditis elegans.
Genetics. 2014 Dec;198(4):1347-56. doi: 10.1534/genetics.114.170423. Epub 2014 Sep 23.
|
co-expression of Cas9 and a sgRNA targeting 5'end of C.elegans K08F4.2
pDD162
|
| AP332-2 | sgRNA for APs4 | U6 |
Seydoux
| Scalable and versatile genome editing using linear DNAs with microhomology to Cas9 Sites in Caenorhabditis elegans.
Genetics. 2014 Dec;198(4):1347-56. doi: 10.1534/genetics.114.170423. Epub 2014 Sep 23.
|
co-expression of Cas9 and a sgRNA targeting 5'end of C.elegans K08F4.2
pDD162
|
| AP332-3 | sgRNA for APs4 | U6 |
Seydoux
| Scalable and versatile genome editing using linear DNAs with microhomology to Cas9 Sites in Caenorhabditis elegans.
Genetics. 2014 Dec;198(4):1347-56. doi: 10.1534/genetics.114.170423. Epub 2014 Sep 23.
|
co-expression of Cas9 and a sgRNA targeting 5'end of C.elegans K08F4.2
pDD162
|
| AP332-4 | sgRNA for APs4 | U6 |
Seydoux
| Scalable and versatile genome editing using linear DNAs with microhomology to Cas9 Sites in Caenorhabditis elegans.
Genetics. 2014 Dec;198(4):1347-56. doi: 10.1534/genetics.114.170423. Epub 2014 Sep 23.
|
co-expression of Cas9 and a sgRNA targeting 5'end of C.elegans K08F4.2
pDD162
|
| AP324-2 | sgRNA for APs6 | U6 |
Seydoux
| Scalable and versatile genome editing using linear DNAs with microhomology to Cas9 Sites in Caenorhabditis elegans.
Genetics. 2014 Dec;198(4):1347-56. doi: 10.1534/genetics.114.170423. Epub 2014 Sep 23.
|
co-expression of Cas9 and a sgRNA targeting 3'end of C.elegans K08F4.2
pDD162
|
| AP324-3 | sgRNA for APs6 | U6 |
Seydoux
| Scalable and versatile genome editing using linear DNAs with microhomology to Cas9 Sites in Caenorhabditis elegans.
Genetics. 2014 Dec;198(4):1347-56. doi: 10.1534/genetics.114.170423. Epub 2014 Sep 23.
|
co-expression of Cas9 and a sgRNA targeting 3'end of C.elegans K08F4.2
pDD162
|
| AP324-4 | sgRNA for APs6 | U6 |
Seydoux
| Scalable and versatile genome editing using linear DNAs with microhomology to Cas9 Sites in Caenorhabditis elegans.
Genetics. 2014 Dec;198(4):1347-56. doi: 10.1534/genetics.114.170423. Epub 2014 Sep 23.
|
co-expression of Cas9 and a sgRNA targeting 3'end of C.elegans K08F4.2
pDD162
|
| AP334-2 | sgRNA for APs5 | U6 |
Seydoux
| Scalable and versatile genome editing using linear DNAs with microhomology to Cas9 Sites in Caenorhabditis elegans.
Genetics. 2014 Dec;198(4):1347-56. doi: 10.1534/genetics.114.170423. Epub 2014 Sep 23.
|
co-expression of Cas9 and a sgRNA targeting 3'end of C.elegans K08F4.2
pDD162
|
| AP334-5 | sgRNA for APs5 | U6 |
Seydoux
| Scalable and versatile genome editing using linear DNAs with microhomology to Cas9 Sites in Caenorhabditis elegans.
Genetics. 2014 Dec;198(4):1347-56. doi: 10.1534/genetics.114.170423. Epub 2014 Sep 23.
|
co-expression of Cas9 and a sgRNA targeting 3'end of C.elegans K08F4.2
pDD162
|
| AP334-6 | sgRNA for APs5 | U6 |
Seydoux
| Scalable and versatile genome editing using linear DNAs with microhomology to Cas9 Sites in Caenorhabditis elegans.
Genetics. 2014 Dec;198(4):1347-56. doi: 10.1534/genetics.114.170423. Epub 2014 Sep 23.
|
co-expression of Cas9 and a sgRNA targeting 3'end of C.elegans K08F4.2
pDD162
|
| APCSD 53 | sgRNA for CSD 53 | U6 |
Seydoux
| Scalable and versatile genome editing using linear DNAs with microhomology to Cas9 Sites in Caenorhabditis elegans.
Genetics. 2014 Dec;198(4):1347-56. doi: 10.1534/genetics.114.170423. Epub 2014 Sep 23.
|
co-expression of Cas9 and a sgRNA targeting 3'end of C.elegans swan-2
pDD162
|
| APCSD 54 | sgRNA for CSD 54 | U6 |
Seydoux
| Scalable and versatile genome editing using linear DNAs with microhomology to Cas9 Sites in Caenorhabditis elegans.
Genetics. 2014 Dec;198(4):1347-56. doi: 10.1534/genetics.114.170423. Epub 2014 Sep 23.
|
co-expression of Cas9 and a sgRNA targeting 3'end of C.elegans swan-2
pDD162
|
| AP568-2 | sgRNA for dpy‐10 | |
Seydoux
| High Efficiency, Homology-Directed Genome Editing in Caenorhabditis elegans Using CRISPR-Cas9 Ribonucleoprotein Complexes.
Genetics. 2015 Sep;201(1):47-54. doi: 10.1534/genetics.115.179382. Epub 2015 Jul 17.
|
co-expression of Cas9 and crRNA 589 target sequence
pDD162
|
| AP568-3 | sgRNA for dpy‐10 | |
Seydoux
| High Efficiency, Homology-Directed Genome Editing in Caenorhabditis elegans Using CRISPR-Cas9 Ribonucleoprotein Complexes.
Genetics. 2015 Sep;201(1):47-54. doi: 10.1534/genetics.115.179382. Epub 2015 Jul 17.
|
co-expression of Cas9 and crRNA 589 target sequence
pDD162
|
| pRB1080 | VQR Cas9 variant (Synthetic) | eef-1A.1 (eft-3) |
Fire
| Cas9 Variants Expand the Target Repertoire in Caenorhabditis elegans.
Genetics. 2015 Dec 17. pii: genetics.115.185041.
|
Expresses VQR Cas9 variant with eef-1A.1 (eft-3) promoter
pCFJ601
|
| pRB1081 | dpy-10 gRNA (Synthetic) | U6 |
Fire
| Cas9 Variants Expand the Target Repertoire in Caenorhabditis elegans.
Genetics. 2015 Dec 17. pii: genetics.115.185041.
|
Expresses gRNA for dpy-10 conversion using NGAG PAM and VQR Cas9 variant
pRB1017
|
| pRB1083 | VRER Cas9 variant (Synthetic) | eft-3 (eef-1A.1) |
Fire
| Cas9 Variants Expand the Target Repertoire in Caenorhabditis elegans.
Genetics. 2015 Dec 17. pii: genetics.115.185041.
|
Expresses VRER Cas9 variant with eef-1A.1 (eft-3) promoter
pCFJ601
|
| pRB1084 | dpy-10 gRNA (Synthetic) | U6 |
Fire
| Cas9 Variants Expand the Target Repertoire in Caenorhabditis elegans.
Genetics. 2015 Dec 17. pii: genetics.115.185041.
|
Expresses gRNA for dpy-10 conversion using NGCG PAM and VRER Cas9 mutant
pRB1017
|
| pUAS:Cas9T2AGFP;U6:sgRNA1;U6:sgRNA2 | 5x UAS (Saccharomyces cerevisiae), Cas9 (Other), T2A, GFP, U6 promoter (Danio rerio) | |
Del Bene
| 2C-Cas9: a versatile tool for clonal analysis of gene function.
Genome Res. 2016 Mar 8.
|
Tissue-specific knock-out in zebrafish, Cas9 and GFP driven by a UAS promoter
pminiTol2
|
| pUAS:Cas9T2ACre;U6:sgRNA1;U6:sgRNA2 | 5xUAS, Cas9, Cre, U6 promoter | |
Del Bene
| 2C-Cas9: a versatile tool for clonal analysis of gene function.
Genome Res. 2016 Mar 8.
|
Tissue-specific knock-out in zebrafish, Cas9 and Cre driven by a UAS promoter
pminiTol2
|
| pDD121 | Cas9 (Synthetic) | |
Goldstein
|
Unpublished SapTrap/SEC plasmids
(unpublished)
|
Cas9 expression in C. elegans
pCFJ601
|
| pCFJ150-Cas9(dpiRNA) | Cas9(dpiRNA) (Caenorhabditis elegans) | eft-3 |
Lee
| The piRNA targeting rules and the resistance to piRNA silencing in endogenous genes.
Science. 2018 Feb 2;359(6375):587-592. doi: 10.1126/science.aao2840. Epub 2018 Feb 1.
|
eft-3p::Cas9(dpiRNA)::tbb-2 3'UTR construct used for MosSCI in nematode. Cas9 is optimized by removing all piRNA targeting sites to allow germline expression.
pCFJ150
|
| Ccas9LacZ | | |
Vellai
| Highly efficient RNAi and Cas9-based auto-cloning systems for C. elegans research.
Nucleic Acids Res. 2018 Jun 19. pii: 5040046. doi: 10.1093/nar/gky516.
|
Enhanced C. elegans Cas9 vector. Ccas9LacZ contains a lacZ coding sequence, two Cas9 and two type-IIs restriction enzyme recognition sites next to the U6 promoter and gRNA sequences.
pDD162 (Peft-3::Cas9 + Empty sgRNA, Addgene no.: #47549)
|