| pC014 - LwCas13a-msfGFP | LwCas13a-msfGFP (Homo sapiens) | EF1a | |
Zhang
| RNA targeting with CRISPR-Cas13.
Nature. 2017 Oct 4. doi: 10.1038/nature24049.
|
Expresses active LwCas13a for mammalian RNA targeting
lenti(AMP)
|
| pC015 - dLwCas13a-NF | dLwCas13a-eGFP-ZF-KRAB (Homo sapiens) | CAG | |
Zhang
| RNA targeting with CRISPR-Cas13.
Nature. 2017 Oct 4. doi: 10.1038/nature24049.
|
Expresses negative-feedback dLwCas13a for mammalian RNA targeting/imaging
pCAGEN
|
| pC016 - LwCas13a guide expression backbone with U6 promoter | LwCas13a Guide site (Synthetic) | | |
Zhang
| RNA targeting with CRISPR-Cas13.
Nature. 2017 Oct 4. doi: 10.1038/nature24049.
|
Backbone for cloning LwCas13a guides under U6 promoter
pUC19
|
| pC017 - LwCas13a guide expression backbone with tRNAval promoter | LwCas13a Guide site (Synthetic) | | |
Zhang
| RNA targeting with CRISPR-Cas13.
Nature. 2017 Oct 4. doi: 10.1038/nature24049.
|
Backbone for cloning LwCas13a guides under tRNAval promoter
pUC19
|
| pC034 - LwCas13a-msfGFP-2A-Blast | LwCas13a-msfGFP (Homo sapiens) | EFS | Blasticidin |
Zhang
| RNA targeting with CRISPR-Cas13.
Nature. 2017 Oct 4. doi: 10.1038/nature24049.
|
Expresses active LwCas13a for mammalian RNA targeting with Blasticidin selection marker
pFUGW
|
| pC035 - dLwCas13a-msfGFP | dLwCas13a-msfGFP (Homo sapiens) | | |
Zhang
| RNA targeting with CRISPR-Cas13.
Nature. 2017 Oct 4. doi: 10.1038/nature24049.
|
Expresses catalytically inactive LwCas13a for mammalian RNA targeting
lenti(AMP)
|
| pC0040-LwaCas13a crRNA backbone | U6-LwaCas13aDR-BbsI-BbsI-polyT | U6 | |
Zhang
| RNA editing with CRISPR-Cas13.
Science. 2017 Oct 25. pii: eaaq0180. doi: 10.1126/science.aaq0180.
|
Backbone for cloning guides that are compatible with LwaCas13a. Contains a 5' direct repeat. Clone using BbsI (BpiI). F overhang AAAC. R overhang AAAA
pUC19
|
| pC0043-PspCas13b crRNA backbone | U6-PspCas13b DR-BbsI-BbsI-polyT | | |
Zhang
| RNA editing with CRISPR-Cas13.
Science. 2017 Oct 25. pii: eaaq0180. doi: 10.1126/science.aaq0180.
|
For cloning of guide RNAs compatible with PspCas13b. Contains a 3' direct repeat. Clone using BbsI (BpiI). F overhang cacc. R overhang caac
pUC19
|
| pC0044-EF1a-RanCas13b-NES-mapk | RanCas13b (Other) | | |
Zhang
| RNA editing with CRISPR-Cas13.
Science. 2017 Oct 25. pii: eaaq0180. doi: 10.1126/science.aaq0180.
|
Expresses RanCas13b in mammalian cells for knockdown of target RNAs in combination with compatible guide RNAs. Localized to the cytoplasm
EF1a-lenti
|
| pC0045-EF1a-PguCas13b-NES-HIV | PguCas13b (Other) | | |
Zhang
| RNA editing with CRISPR-Cas13.
Science. 2017 Oct 25. pii: eaaq0180. doi: 10.1126/science.aaq0180.
|
Expresses PguCas13b in mammalian cells for knockdown of target RNAs in combination with compatible guide RNAs. Localized to the cytoplasm
EF1a-lenti
|
| pC0046-EF1a-PspCas13b-NES-HIV | PspCas13b (Other) | | |
Zhang
| RNA editing with CRISPR-Cas13.
Science. 2017 Oct 25. pii: eaaq0180. doi: 10.1126/science.aaq0180.
|
Expresses PspCas13b in mammalian cells for knockdown of target RNAs in combination with compatible guide RNAs. Localized to the cytoplasm
EF1a-lenti
|
| pC0049-EF1a-dPSPCas13b-NES-HIV, H133A/H1058A | PspCas13b (Other) | EF1a | |
Zhang
| RNA editing with CRISPR-Cas13.
Science. 2017 Oct 25. pii: eaaq0180. doi: 10.1126/science.aaq0180.
|
Catalytically inactive PSPCas13b. RNase activity is inactivated by mutation of catalytic histidine residues in the HEPN domains (H133A/H1058A).
Unknown
|
| pC0056 - LwCas13a-msfGFP-NES | LwCas13a-msfGFP-NES (Homo sapiens) | | |
Zhang
| RNA targeting with CRISPR-Cas13.
Nature. 2017 Oct 4. doi: 10.1038/nature24049.
|
Expresses active LwaCas13a-NES for mammalian RNA targeting
lenti(AMP)
|
| pXR001: EF1a-CasRx-2A-EGFP | CasRx (Homo sapiens) | EF1A | GFP |
Hsu
| Transcriptome Engineering with RNA-Targeting Type VI-D CRISPR Effectors.
Cell. 2018 Mar 8. pii: S0092-8674(18)30207-1. doi: 10.1016/j.cell.2018.02.033.
|
CasRx (NLS-RfxCas13d-NLS) with 2A-EGFP for RNA targeting
lenti(AMP)
|
| pXR002: EF1a-dCasRx-2A-EGFP | dCasRx (Homo sapiens) | | GFP |
Hsu
| Transcriptome Engineering with RNA-Targeting Type VI-D CRISPR Effectors.
Cell. 2018 Mar 8. pii: S0092-8674(18)30207-1. doi: 10.1016/j.cell.2018.02.033.
|
Catalytically inactive dCasRx with 2A-EGFP
lenti(AMP)
|
| pAC1801_pmax-dCasRx | dCasRx (Synthetic) | CAGGS | |
Cheng
| CRISPR Artificial Splicing Factors
bioRxiv 431064
|
Transient transfection; Expresses dCasRx; CAGGS promoter
pMAX
|
| pAC1802_pmax-RBFOX1N-dCasRx-C | RBFOX1N-dCasRx-C (Synthetic) | CAGGS | |
Cheng
| CRISPR Artificial Splicing Factors
bioRxiv 431064
|
Transient transfection; Expresses RBFOX1N-dCasRx-C; CAGGS promoter
pMAX
|
| pAC1803_pmax-RBM38-dCasRx | RBM38-dCasRx (Synthetic) | CAGGS | |
Cheng
| CRISPR Artificial Splicing Factors
bioRxiv 431064
|
Transient transfection; Expresses RBM38-dCasRx; CAGGS promoter
pMAX
|
| pAC1804_pmax-dCasRx-RBM38 | dCasRx-RBM38 (Synthetic) | CAGGS | |
Cheng
| CRISPR Artificial Splicing Factors
bioRxiv 431064
|
Transient transfection; Expresses dCasRx-RBM38; CAGGS promoter
pMAX
|
| pAC1806_pmax-FKBP-dCasRx | FKBP-dCasRx (Synthetic) | CAGGS | |
Cheng
| CRISPR Artificial Splicing Factors
bioRxiv 431064
|
Transient transfection; Expresses FKBP-dCasRx; CAGGS promoter
pMAX
|
| pAC1807_pmax-dCasRx-FKBP | dCasRx-FKBP (Synthetic) | CAGGS | |
Cheng
| CRISPR Artificial Splicing Factors
bioRxiv 431064
|
Transient transfection; Expresses dCasRx-FKBP; CAGGS promoter
pMAX
|
| Cmv d0 dPspCas13b-GGS-NYTHDF1 | dCas13b-GGS-NYTHDF1 | | |
Dickinson
| Targeted m(6)A Reader Proteins To Study Epitranscriptomic Regulation of Single RNAs.
J Am Chem Soc. 2018 Sep 26;140(38):11974-11981. doi: 10.1021/jacs.8b05012. Epub 2018 Sep 18.
|
Cmv d0 dPspCas13b-GGS-NYTHDF1 fusion protein (full N-terminal domain of YTHDF1, C-terminal YTH domain omitted)
cmv d0
|
| Cmv d0 dPspCas13b-GGS-NYTHDF2(100-200) | dCas13b-GGS-NYTHDF2(100-200) | | |
Dickinson
| Targeted m(6)A Reader Proteins To Study Epitranscriptomic Regulation of Single RNAs.
J Am Chem Soc. 2018 Sep 26;140(38):11974-11981. doi: 10.1021/jacs.8b05012. Epub 2018 Sep 18.
|
Cmv d0 dPspCas13b-GGS-NYTHDF2(100-200) (YTDHF2 100-200 subdomain)
cmv d0
|
| Cmv d0 dPspCas13b-GGS-NYTHDF1(100-200) | dCas13b-GGS-NYTHDF1(100-200) | | |
Dickinson
| Targeted m(6)A Reader Proteins To Study Epitranscriptomic Regulation of Single RNAs.
J Am Chem Soc. 2018 Sep 26;140(38):11974-11981. doi: 10.1021/jacs.8b05012. Epub 2018 Sep 18.
|
Cmv d0 dPspCas13b-GGS-NYTHDF1(100-200) (YTHDF1 100-200 subdomain)
cmv d0
|
| Cmv d0 dPspCas13b-GGS-Sun | dCas13b-GGS-Sun | | |
Dickinson
| Targeted m(6)A Reader Proteins To Study Epitranscriptomic Regulation of Single RNAs.
J Am Chem Soc. 2018 Sep 26;140(38):11974-11981. doi: 10.1021/jacs.8b05012. Epub 2018 Sep 18.
|
Cmv d0 dPspCas13b-GGS-Sun no-fusion construct
cmv d0
|