CRISPR Plasmids: Nick
CRISPR/Cas nickase mutants introduce gRNA-targeted single-strand breaks in DNA instead of the double-strand breaks created by wild type Cas enzymes. To use a nickase mutant, you will need two gRNAs that target opposite strands of your DNA in close proximity. These double nicks create a double-strand break (DSB) that is repaired using error-prone non-homologous end joining (NHEJ). Double nicking strategies reduce unwanted off-target effects. Nickase mutants can also be used with a repair template to introduce specific edits via homology-directed repair (HDR).
Want more information on the wide variety of Cas enzymes? CasPEDIA is an encyclopedia of Class 2 CRISPR systems with wiki entries describing enzyme activity, experimental considerations, and more, created and maintained by the Doudna Lab.
Browse, sort, or search the tables below for CRISPR nickase plasmids.Plasmids are available for expression in mammalian systems, bacteria, Drosophila, plants, and yeast.
Mammalian
| ID | Plasmid | Gene/Insert | Promoter | Selectable Marker | PI | Publication |
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Bacteria
| ID | Plasmid | Gene/Insert | Promoter | Selectable Marker | PI | Publication |
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Drosophila
| ID | Plasmid | Gene/Insert | Promoter | Selectable Marker | PI | Publication |
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Plant
| ID | Plasmid | Gene/Insert | Promoter | Selectable Marker | PI | Publication |
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Yeast
| ID | Plasmid | Description | Gene/Insert | Promoter | Selectable Marker | PI | Publication |
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