| pBUN501 | zCas9D10A (Synthetic), gRNA scaffold (Synthetic) | Ubi1p, AtU6-26p | Bar |
Chen
| A CRISPR/Cas9 toolkit for multiplex genome editing in plants.
BMC Plant Biol. 2014 Nov 29;14(1):327.
|
CRISPR/Cas based plant genome editing and gene regulation; expresses zCas9D10A, gRNA scaffold for insertion of target sequence (AtU6-26 promoter), Bar resistance
pGreen-like binary vector
|
| pHSN501 | zCas9D10A (Synthetic), gRNA scaffold (Synthetic) | 2×35Sp, AtU6-26p | Hygromycin |
Chen
| A CRISPR/Cas9 toolkit for multiplex genome editing in plants.
BMC Plant Biol. 2014 Nov 29;14(1):327.
|
CRISPR/Cas based plant genome editing and gene regulation; expresses zCas9D10A, gRNA scaffold for insertion of target sequence (AtU6-26 promoter), Hyg resistance
pGreen-like binary vector
|
| pDe-CAS9-D10A | Cas9-D10A (Arabidopsis thaliana) | PcUbi4-2 | Kanamycin |
Puchta
| Both CRISPR/Cas-based nucleases and nickases can be used efficiently for genome engineering in Arabidopsis thaliana.
Plant J. 2014 Jul;79(2):348-59. doi: 10.1111/tpj.12554. Epub 2014 Jun 17.
|
based on pDe-CAS9, encodes Cas9 D10A nickase variant
pPZP221
|
| pYPQ159 | hSpCas9D10A (Human codon optimized) (Other) | | |
Qi
| A CRISPR/Cas9 toolbox for multiplexed plant genome editing and transcriptional regulation.
Plant Physiol. 2015 Aug 21. pii: pp.00636.2015.
|
Gateway entry vector with hSpCas9D10A
pNJB185-linker6
|
| pDGE76 | pnos:nptII-tmos (Other), p2x35S:Cas9 D10A-tnos (Other), BsaI-ccdB_CmR-BsaI | | |
Stuttmann
| Generation of chromosomal deletions in dicotyledonous plants employing a user-friendly genome editing toolkit.
Plant J. 2016 Aug 31. doi: 10.1111/tpj.13319.
|
recipient plasmid [multiplexing, nickase]; p35S:Cas9 D10A, nptII
pVM_BGW
|
| pDGE77 | pnos:PAT-tnos (Other), p2x35S:Cas9 D10A-tnos (Other), BsaI-ccdB_CmR-BsaI | | |
Stuttmann
| Generation of chromosomal deletions in dicotyledonous plants employing a user-friendly genome editing toolkit.
Plant J. 2016 Aug 31. doi: 10.1111/tpj.13319.
|
recipient plasmid [multiplexing, nickase]; p35S:Cas9 D10A, BASTA
pVM_BGW
|
| pDGE78 | pnos:nptII-tmos (Other), pPcUbi:Cas9 D10A-tnos (Other), BsaI-ccdB_CmR-BsaI | | |
Stuttmann
| Generation of chromosomal deletions in dicotyledonous plants employing a user-friendly genome editing toolkit.
Plant J. 2016 Aug 31. doi: 10.1111/tpj.13319.
|
recipient plasmid [multiplexing, nickase]; pPcUbi:Cas9 D10A, nptII
pVM_BGW
|
| pDGE79 | pnos:PAT-tnos (Other), pPcUbi:Cas9 D10A-tnos (Other), BsaI-ccdB_CmR-BsaI | | |
Stuttmann
| Generation of chromosomal deletions in dicotyledonous plants employing a user-friendly genome editing toolkit.
Plant J. 2016 Aug 31. doi: 10.1111/tpj.13319.
|
recipient plasmid [multiplexing, nickase]; pPcUbi:Cas9 D10A, BASTA
pVM_BGW
|
| pTX179 | | | Hygromycin |
Voytas
| A Single Transcript CRISPR-Cas9 System for Efficient Genome Editing in Plants.
Mol Plant. 2016 Jul 6;9(7):1088-91. doi: 10.1016/j.molp.2016.05.001. Epub 2016 May 19.
|
Express STU (Single Transcriptional Unit) Cas9 nickase with Maize Ubiquitin1 promoter in plants
pTX172
Tag
/ Fusion Protein- SV40 NLS (C terminal on insert)
|
| pMOD_A0201 | AtCas9_D10A nickase (Synthetic) | 35S | |
Voytas
| A multi-purpose toolkit to enable advanced genome engineering in plants.
Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922.
|
Module A, Promoter: 35S, Gene: AtCas9_D10A nickase, Terminator: HSP
pMOD_A1001
|
| pMOD_A0202 | AtCas9_D10A nickase (Synthetic) | AtUbi10 | |
Voytas
| A multi-purpose toolkit to enable advanced genome engineering in plants.
Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922.
|
Module A, Promoter: AtUbi10, Gene: AtCas9_D10A nickase, Terminator: HSP
pMOD_A1001
|
| pMOD_A0208 | AtCas9_D10A nickase (Synthetic) | EC1.2 | |
Voytas
| A multi-purpose toolkit to enable advanced genome engineering in plants.
Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922.
|
Module A, Promoter: EC1.2, Gene: AtCas9_D10A nickase, Terminator: HSP
pMOD_A1001
|
| pMOD_A0301 | AtCas9_H840A nickase (Synthetic) | 35S | |
Voytas
| A multi-purpose toolkit to enable advanced genome engineering in plants.
Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922.
|
Module A, Promoter: 35S, Gene: AtCas9_H840A nickase, Terminator: HSP
pMOD_A1001
|
| pMOD_A0302 | AtCas9_H840A nickase (Synthetic) | AtUbi10 | |
Voytas
| A multi-purpose toolkit to enable advanced genome engineering in plants.
Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922.
|
Module A, Promoter: AtUbi10, Gene: AtCas9_H840A nickase, Terminator: HSP
pMOD_A1001
|
| pMOD_A0308 | AtCas9_H840A nickase (Synthetic) | EC1.2 | |
Voytas
| A multi-purpose toolkit to enable advanced genome engineering in plants.
Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922.
|
Module A, Promoter: EC1.2, Gene: AtCas9_H840A nickase, Terminator: HSP
pMOD_A1001
|
| pMOD_A0601 | Csy4-P2A-AtCas9_D10A nickase (Synthetic) | 35S | |
Voytas
| A multi-purpose toolkit to enable advanced genome engineering in plants.
Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922.
|
Module A, Promoter: 35S, Gene: Csy4-P2A-AtCas9_D10A nickase, Terminator: HSP
pMOD_A1001
|
| pMOD_A0608 | Csy4-P2A-AtCas9_D10A nickase (Synthetic) | EC1.2 | |
Voytas
| A multi-purpose toolkit to enable advanced genome engineering in plants.
Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922.
|
Module A, Promoter: EC1.2, Gene: Csy4-P2A-AtCas9_D10A nickase, Terminator: HSP
pMOD_A1001
|
| pMOD_A0701 | Csy4-P2A-AtCas9_H840A nickase (Synthetic) | 35S | |
Voytas
| A multi-purpose toolkit to enable advanced genome engineering in plants.
Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922.
|
Module A, Promoter: 35S, Gene: Csy4-P2A-AtCas9_H840A nickase, Terminator: HSP
pMOD_A1001
|
| pMOD_A0708 | Csy4-P2A-AtCas9_H840A nickase (Synthetic) | EC1.2 | |
Voytas
| A multi-purpose toolkit to enable advanced genome engineering in plants.
Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922.
|
Module A, Promoter: EC1.2, Gene: Csy4-P2A-AtCas9_H840A nickase, Terminator: HSP
pMOD_A1001
|
| pMOD_A1210 | TaCas9_D10A nickase (Synthetic) | ZmUbi | |
Voytas
| A multi-purpose toolkit to enable advanced genome engineering in plants.
Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922.
|
Module A, Promoter: ZmUbi, Gene: TaCas9_D10A nickase, Terminator: HSP
pMOD_A1001
|
| pMOD_A1310 | TaCas9_H840A nickase (Synthetic) | ZmUbi | |
Voytas
| A multi-purpose toolkit to enable advanced genome engineering in plants.
Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922.
|
Module A, Promoter: ZmUbi, Gene: TaCas9_H840A nickase, Terminator: HSP
pMOD_A1001
|
| pMOD_A1610 | Csy4-P2A-TaCas9_D10A nickase (Synthetic) | ZmUbi | |
Voytas
| A multi-purpose toolkit to enable advanced genome engineering in plants.
Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922.
|
Module A, Promoter: ZmUbi, Gene: Csy4-P2A-TaCas9_D10A nickase, Terminator: HSP
pMOD_A1001
|
| pMOD_A1710 | Csy4-P2A-TaCas9_H840A nickase (Synthetic) | ZmUbi | |
Voytas
| A multi-purpose toolkit to enable advanced genome engineering in plants.
Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922.
|
Module A, Promoter: ZmUbi, Gene: Csy4-P2A-TaCas9_H840A nickase, Terminator: HSP
pMOD_A1001
|
| pAtCas9-D10A_1 | AtCas9_D10A (nickase) (Synthetic) | | Hygromycin |
Voytas
| A multi-purpose toolkit to enable advanced genome engineering in plants.
Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922.
|
Cas9 only expression, Cas9 Type: AtCas9_D10A (nickase), Plant Selection: 2x35S:hpt II
pCAMBIA
|
| pAtCas9-D10A_2 | AtCas9_D10A (nickase) (Synthetic) | | Kanamycin |
Voytas
| A multi-purpose toolkit to enable advanced genome engineering in plants.
Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922.
|
Cas9 only expression, Cas9 Type: AtCas9_D10A (nickase), Plant Selection: 2x35S:npt II
pCAMBIA
|
| pAtCas9-D10A_3 | AtCas9_D10A (nickase) (Synthetic) | | Basta |
Voytas
| A multi-purpose toolkit to enable advanced genome engineering in plants.
Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922.
|
Cas9 only expression, Cas9 Type: AtCas9_D10A (nickase), Plant Selection: 2x35S:bar
pCAMBIA
|
| pAtCas9-H840A_1 | AtCas9_H840A (nickase) (Synthetic) | | Hygromycin |
Voytas
| A multi-purpose toolkit to enable advanced genome engineering in plants.
Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922.
|
Cas9 only expression, Cas9 Type: AtCas9_H840A (nickase), Plant Selection: 2x35S:hpt II
pCAMBIA
|
| pAtCas9-H840A_2 | AtCas9_H840A (nickase) (Synthetic) | | Kanamycin |
Voytas
| A multi-purpose toolkit to enable advanced genome engineering in plants.
Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922.
|
Cas9 only expression, Cas9 Type: AtCas9_H840A (nickase), Plant Selection: 2x35S:npt II
pCAMBIA
|
| pTaCas9-D10A_5 | TaCas9_D10A (nickase) (Synthetic) | | Hygromycin |
Voytas
| A multi-purpose toolkit to enable advanced genome engineering in plants.
Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922.
|
Cas9 only expression, Cas9 Type: TaCas9_D10A (nickase), Plant Selection: PvUbi2:hpt II
pCAMBIA
|
| pTaCas9-D10A_6 | TaCas9_D10A (nickase) (Synthetic) | | Basta |
Voytas
| A multi-purpose toolkit to enable advanced genome engineering in plants.
Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922.
|
Cas9 only expression, Cas9 Type: TaCas9_D10A (nickase), Plant Selection: PvUbi2:bar
pCAMBIA
|
| pTaCas9-H840A_5 | TaCas9_H840A (nickase) (Synthetic) | | Hygromycin |
Voytas
| A multi-purpose toolkit to enable advanced genome engineering in plants.
Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922.
|
Cas9 only expression, Cas9 Type: TaCas9_H840A (nickase), Plant Selection: PvUbi2:hpt II
pCAMBIA
|
| pTaCas9-H840A_6 | TaCas9_H840A (nickase) (Synthetic) | | Basta |
Voytas
| A multi-purpose toolkit to enable advanced genome engineering in plants.
Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922.
|
Cas9 only expression, Cas9 Type: TaCas9_H840A (nickase), Plant Selection: PvUbi2:bar
pCAMBIA
|
| pJG363 | nAtCas9_D10A+gNt_R2+donor (Other) | | |
Voytas
| A multi-purpose toolkit to enable advanced genome engineering in plants.
Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922.
|
BeYDV replicon T-DNA for gene targeting in tobacco leaves, D10A single nickase (nAtCas9_D10A+gNt_R2+donor)
pTRANS_201
|
| pJG367 | nAtCas9_H840A+gNt_R2+gNt_F2+donor (Other) | | |
Voytas
| A multi-purpose toolkit to enable advanced genome engineering in plants.
Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922.
|
BeYDV replicon T-DNA for gene targeting in tobacco leaves, H840A double nickase (nAtCas9_H840A+gNt_R2+gNt_F2+donor)
pTRANS_201
|
| pJG370 | nAtCas9_D10A+gRNActrl+donor (Synthetic) | | |
Voytas
| A multi-purpose toolkit to enable advanced genome engineering in plants.
Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922.
|
BeYDV replicon T-DNA for gene targeting in tobacco leaves, D10A single nickase no gRNA control (nAtCas9_D10A+gRNActrl+donor)
pTRANS_201
|
| pJG382 | nAtCas9_D10A+gNt_R2+gNt_F2+donor (Other) | | |
Voytas
| A multi-purpose toolkit to enable advanced genome engineering in plants.
Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922.
|
BeYDV replicon T-DNA for gene targeting in tobacco leaves, D10A double nickase (nAtCas9_D10A+gNt_R2+gNt_F2+donor)
pTRANS_201
|
| pJG484 | nTaCas9_H840A+gUbi8+donor (Other) | | |
Voytas
| A multi-purpose toolkit to enable advanced genome engineering in plants.
Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922.
|
WDV replicon T-DNA for gene targeting in wheat scutella, H840A single nickase (nTaCas9_H840A+gUbi8+donor)
pTRANS_203
|
| pJG486 | nTaCas9_H840A+gUbi1+donor (Other) | | |
Voytas
| A multi-purpose toolkit to enable advanced genome engineering in plants.
Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922.
|
WDV replicon T-DNA for gene targeting in wheat scutella, H840A single nickase (nTaCas9_H840A+gUbi1+donor)
pTRANS_203
|
| pJG488 | nTaCas9_H840A+gUbi8+gUbi1+donor (Other) | | |
Voytas
| A multi-purpose toolkit to enable advanced genome engineering in plants.
Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922.
|
WDV replicon T-DNA for gene targeting in wheat scutella, H840A double nickase (nTaCas9_H840A+gUbi8+gUbi1+donor)
pTRANS_203
|
| pORFE0002 | AtCAS9 nickase D10A (Other) | | |
Chevalier
|
Chevalier Lab plasmids
(unpublished)
|
AtCAS9 nickase D10A module CDS1
pICH41308
|
| pORFE0014 | AtCAS9 D10A without stop codon (Other) | | |
Chevalier
|
Chevalier Lab plasmids
(unpublished)
|
AtCAS9 nickase D10A module CDS1ns
pAGM1287
|
| pORFE1052 | AtCAS9 D10A (Other) | 2x35S | |
Chevalier
|
Chevalier Lab plasmids
(unpublished)
|
Overexpression of the AtCAS9 nickase in plant under 2x35S promoter
pICH47742
|
| pFH91 | nScCas9 (nickase), wheat codon optimized (Synthetic) | | |
Nekrasov
| A modular cloning toolkit for genome editing in plants.
BMC Plant Biol. 2020 Apr 23;20(1):179. doi: 10.1186/s12870-020-02388-2.
|
Level0 nScCas9 (nickase), wheat codon optimized
pICH41308
|
| pFH109 | ZmUBIp::nScCas9 (wheat codon optimized):NOSt (Synthetic) | | |
Nekrasov
| A modular cloning toolkit for genome editing in plants.
BMC Plant Biol. 2020 Apr 23;20(1):179. doi: 10.1186/s12870-020-02388-2.
|
Level1 ZmUBIp::nScCas9 (wheat codon optimized):NOSt
pICH47742
|