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gRNA_Cloning Vector
(Plasmid #41824)

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Available to Academic and Nonprofits Only

Backbone

  • Vector backbone
    pCR-Blunt II-TOPO
  • Backbone manufacturer
    Invitrogen
  • Vector type
    Mammalian Expression, CRISPR
  • Tag / Fusion Protein
    • AflII site for Gibson Assembly

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Top10
  • Copy number
    Unknown

Sequence Information

Full plasmid sequence is available only if provided by the depositing laboratory.

Cloning Information

Resource Information

Depositor Comments

For more information on Church Lab CRISPR Plasmids please refer to: http://www.addgene.org/crispr/church/

How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    gRNA_Cloning Vector was a gift from George Church (Addgene plasmid # 41824)
  • For your References section:

    RNA-Guided Human Genome Engineering via Cas9. Mali P, Yang L, Esvelt KM, Aach J, Guell M, Dicarlo JE, Norville JE, Church GM. Science. 2013 Jan 3. 10.1126/science.1232033 PubMed 23287722