An empty gRNA expression vector, used to create a gRNA to a specific sequence.
( Search Vector Database)
5' sequencing primer:
List of Sequencing Primers
Fusion protein or tag:
AflII site for Gibson Assembly
Growth temperature (℃):
High or low copy:
View sequences (3)
hCRISPR gRNA Synthesis Protocol (application/pdf)
Terms and Licenses:
sequenced a portion of this plasmid for verification.
Full plasmid sequence is available only if provided by the depositing laboratory.
Please acknowledge the principal investigator and cite this article if you use
this plasmid in a publication. Also, please include the text "Addgene plasmid
41824" in your Materials and Methods section.