Plasmid 41824: gRNA_Cloning Vector
  • An empty gRNA expression vector, used to create a gRNA to a specific sequence.

  • None

  • pCR-Blunt II-TOPO
    (Search Vector Database)

  • Invitrogen


  • T7 List of Sequencing Primers

  • AflII site for Gibson Assembly

  • Kanamycin

  • Top10

  • 37

  • Unknown

  • View sequences (3)
  • hCRISPR gRNA Synthesis Protocol (application/pdf)

  • George Church



For more information on Church Lab CRISPR Plasmids please refer to: http://www.addgene.org/crispr/church/

Addgene has sequenced a portion of this plasmid for verification. Full plasmid sequence is available only if provided by the depositing laboratory.

Article: RNA-Guided Human Genome Engineering via Cas9. Mali et al (Science. 2013 Jan 3. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 41824" in your Materials and Methods section.