An empty gRNA expression vector, used to create a gRNA to a specific sequence.
( Search Vector Database)
5' sequencing primer:
List of Sequencing Primers
Fusion protein or tag:
AflII site for Gibson Assembly
Growth temperature (℃):
High or low copy:
View sequences (3)
hCRISPR gRNA Synthesis Protocol (application/pdf)
Terms and Licenses:
Addgene has sequenced a portion of this plasmid for verification.
here for the sequencing
Please acknowledge the principal investigator and cite this article if you use
this plasmid in a publication. Also, please include the text "Addgene plasmid
41824" in your Materials and Methods section.