gRNA_Cloning Vector
(Plasmid #41824)

• Purpose: (Empty Backbone) An empty gRNA expression vector, used to create a gRNA to a specific sequence.
• Depositing Lab: George Church
• Publication: Mali et al Science. 2013 Jan 3.
• Sequence Information:

  • Depositor Sequences: Partial (2)
  • Addgene Sequences: Full (1) Partial (1)

Ordering

Item	Catalog #	Description	Quantity	Price (USD)
Plasmid	41824	Plasmid sent as bacteria in agar stab	1	$65	Add to Cart

This material is available to academics and nonprofits only.

Backbone

• Vector backbone: pCR-Blunt II-TOPO
  (Search Vector Database)
• Backbone manufacturer: Invitrogen
• Vector type: Mammalian Expression, CRISPR
• Tag / Fusion Protein:
  • AflII site for Gibson Assembly

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin
• Growth Temperature: 37°C
• Growth Strain(s): Top10
• Copy number: Unknown

Cloning Information

• Cloning method: TOPO Cloning
• 5′ sequencing primer: T7

Resource Information

• Supplemental Documents:
  • hCRISPR gRNA Synthesis Protocol
• Terms and Licenses:
  • UBMTA
• Articles Citing this Plasmid:
  • 104 References

Depositor Comments

For more information on Church Lab CRISPR Plasmids please refer to: http://www.addgene.org/crispr/church/