Vector backbonepCR-Blunt II-TOPO
Vector typeMammalian Expression, CRISPR
/ Fusion Protein
- AflII site for Gibson Assembly
Growth in Bacteria
Full plasmid sequence is available only if provided by the depositing laboratory.
For more information on Church Lab CRISPR Plasmids please refer to: http://www.addgene.org/crispr/church/
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:gRNA_Cloning Vector was a gift from George Church (Addgene plasmid # 41824)
For your References section:RNA-Guided Human Genome Engineering via Cas9. Mali P, Yang L, Esvelt KM, Aach J, Guell M, Dicarlo JE, Norville JE, Church GM. Science. 2013 Jan 3. 10.1126/science.1232033 PubMed 23287722
Map generated by Addgene from plasmid data.