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Purpose(Empty Backbone) An empty gRNA expression vector, used to create a gRNA to a specific sequence.
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | ||
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Plasmid | 41824 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $65 |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepCR-Blunt II-TOPO
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Backbone manufacturerInvitrogen
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Vector typeMammalian Expression, CRISPR
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Tag
/ Fusion Protein
- AflII site for Gibson Assembly
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin
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Growth Temperature37°C
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Growth Strain(s)Top10
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Copy numberUnknown
Cloning Information
- Cloning method TOPO Cloning
- 5′ sequencing primer T7 (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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Terms and Licenses
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Articles Citing this Plasmid
Depositor Comments
For more information on Church Lab CRISPR Plasmids please refer to: http://www.addgene.org/crispr/church/
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
gRNA_Cloning Vector was a gift from George Church (Addgene plasmid # 41824 ; http://n2t.net/addgene:41824 ; RRID:Addgene_41824) -
For your References section:
RNA-Guided Human Genome Engineering via Cas9. Mali P, Yang L, Esvelt KM, Aach J, Guell M, Dicarlo JE, Norville JE, Church GM. Science. 2013 Jan 3. 10.1126/science.1232033 PubMed 23287722