gRNA_Cloning Vector
(Plasmid #41824)

• Purpose: (Empty Backbone) An empty gRNA expression vector, used to create a gRNA to a specific sequence.
• Depositing Lab: George Church
• Publication: Mali et al Science. 2013 Feb 15;339(6121):823-6. doi: 10.1126/science.1232033

Backbone

• Vector backbone: pCR-Blunt II-TOPO
• Backbone manufacturer: Invitrogen
• Vector type: Mammalian Expression, CRISPR
• Tag / Fusion Protein:
  • AflII site for Gibson Assembly

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Top10
• Copy number: Unknown

Cloning Information

• Cloning method: TOPO Cloning
• 5′ sequencing primer: T7

Resource Information

• Supplemental Documents:
  • hCRISPR gRNA Synthesis Protocol
• Articles Citing this Plasmid:
  • 345 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

For more information on Church Lab CRISPR Plasmids please refer to: http://www.addgene.org/crispr/church/

Information for Cloning Grade DNA (Catalog # 41824-DNA.cg) ( Back to top)

Purpose

Cloning grade DNA is suitable for use in PCR, cloning reactions, or transformation into E. coli. The purity and amount is not suitable for direct transfections.

Delivery

• Amount: 2 µg
• Guaranteed Concentration: 100 ng/µl +/- 5 ng/µl
• Pricing: $105 USD
• Storage: DNA can be stored at 4℃ (short term) or -20℃ (long term).

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Quality Control

Addgene has verified this plasmid using Next Generation Sequencing. Results are available here