Addgene - gRNA_Cloning Vector Plasmid Data

Plasmid 41824: gRNA_Cloning Vector

Purpose:
An empty gRNA expression vector, used to create a gRNA to a specific sequence.
Gene/insert name:
None
Vector backbone:
pCR-Blunt II-TOPO
(Search Vector Database)
Backbone manufacturer:
Invitrogen
Vector type:
CRISPR
5' sequencing primer:
T7 List of Sequencing Primers
Fusion protein or tag:
AflII site for Gibson Assembly
Bacterial resistance(s)
Kanamycin
Growth strain(s)
Top10
Growth temperature (℃):
37
High or low copy:
Unknown
Sequence: View sequences (3)
Supplemental document:
hCRISPR gRNA Synthesis Protocol (application/pdf)
Principal Investigator:
George Church
Terms and Licenses:
UBMTA

Comments:

For more information on Church Lab CRISPR Plasmids please refer to: http://www.addgene.org/crispr/church/

Addgene has sequenced a portion of this plasmid for verification. Full plasmid sequence is available only if provided by the depositing laboratory.

Article: RNA-Guided Human Genome Engineering via Cas9. Mali et al (Science. 2013 Jan 3. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 41824" in your Materials and Methods section.