gRNA_Cloning Vector
(Plasmid #41824)

• Purpose: (Empty Backbone) An empty gRNA expression vector, used to create a gRNA to a specific sequence.
• Depositing Lab: George Church
• Publication: Mali et al Science. 2013 Jan 3.
• Price: $65 (USD)

Backbone

• Vector backbone: pCR-Blunt II-TOPO
  (Search Vector Database)
• Backbone manufacturer: Invitrogen
• Vector type: Mammalian Expression, CRISPR
• Tag / Fusion Protein:
  • AflII site for Gibson Assembly

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin
• Growth Temperature: 37°C
• Growth Strain(s): Top10
• Copy number: Unknown

Sequence Information

• Depositor Sequences:
  Partial (2)
• Addgene Sequences:
  Partial (1)

Full plasmid sequence is available only if provided by the depositing laboratory.

Cloning Information

• Cloning method: TOPO Cloning
• 5′ sequencing primer: T7

Resource Information

• Supplemental documents:
  • hCRISPR gRNA Synthesis Protocol
• Terms and Licenses:
  • UBMTA