|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||42333||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector backbonepUC ori vector
Vector typeMammalian Expression, CRISPR
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert namehumanized S. pyogenes Cas9 (D10A) nickase
Alt nameSpCas9n (D10A)
Alt namehSpCas9n (D10A)
Insert Size (bp)4272
MutationD10A nickase-converting mutation
/ Fusion Protein
- HA (N terminal on insert)
Note, Addgene's quality control sequencing has found a few discrepancies with the depositor's full sequence. The depositing lab is aware of these discrepancies, and they are not thought to affect plasmid function.
For more information on Zhang Lab CRISPR Plasmids please refer to: http://www.addgene.org/crispr/zhang/
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pX334-U6-DR-BB-DR-Cbh-NLS-hSpCas9n(D10A)-NLS-H1-shorttracr-PGK-puro was a gift from Feng Zhang (Addgene plasmid # 42333 ; http://n2t.net/addgene:42333 ; RRID:Addgene_42333)
For your References section:Multiplex Genome Engineering Using CRISPR/Cas Systems. Cong L, Ran FA, Cox D, Lin S, Barretto R, Habib N, Hsu PD, Wu X, Jiang W, Marraffini LA, Zhang F. Science. 2013 Jan 3. 10.1126/science.1231143 PubMed 23287718