CRISPR Plasmids: Epigenetics
To make targeted epigenetic modifications, researchers have fused catalytically dead dCas9 to epigenetic modifiers. Design your gRNA to target a specific promoter or enhancer for your gene of interest.
Available modifications include:
- histone acetylation by p300
- histone demethylation by LSD1
- cytosine methylation by DNMT3A or MQ1
- cytosine demethylation by Tet1
These modifications persist over time and are potentially heritable in dividing cells. CRISPR Activate and Interfere technologies also indirectly modify local chromatin state.
Want more information on the wide variety of Cas enzymes? CasPEDIA is an encyclopedia of Class 2 CRISPR systems with wiki entries describing enzyme activity, experimental considerations, and more, created and maintained by the Doudna Lab.
Browse, sort, or search the tables below for CRISPR plasmids for epigenetic modification in mammalian or plant systems.
Mammalian
| ID | Plasmid | Gene/Insert | Promoter | Selectable Marker | PI | Publication |
|---|
Plant
| ID | Plasmid | Gene/Insert | Promoter | Selectable Marker | PI | Publication |
|---|
Do you have suggestions for other plasmids that should be added to this list?
Fill out our Suggest a Plasmid form or e-mail [email protected] to help us improve this resource!