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CRISPR header icon CRISPR/Cas Plasmids: Label Genomic Loci

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A catalytically inactive Cas9 (dCas9) fused to a fluorescent protein (FP) can be used to visualize specific genomic loci using fluorescent microscopy in living cells. Design your gRNA sequence to direct the dCas9-FP fusion to a specific genomic sequence. Potential target locations can include unique or repetitive regions. If the plasmid that you choose does not also express a gRNA, you will need to use a separate gRNA expression plasmid to target the dCas9-FP protein.




Mammalian

Plasmid Gene/Insert Promoter Selectable Marker PI Publication Hidden Extra Search Info
pHR-SFFV-dCas9-BFPdCas9-BFP fusion (Homo sapiens)SFFV Weissman CRISPR-Mediated Modular RNA-Guided Regulation of Transcription in Eukaryotes. Cell. 2013 Jul 9. pii: S0092-8674(13)00826-X. doi: 10.1016/j.cell.2013.06.044. Human expression vector containing SFFV promoter, dCas9 that is fused to 2x NLS and tagBFP pHR
pSLQ1658-dCas9-EGFPdCas9 fuse to EGFP (Homo sapiens)MSCV LTR promoterPuromycin Qi Dynamic Imaging of Genomic Loci in Living Human Cells by an Optimized CRISPR/Cas System. Cell. 2013 Dec 19;155(7):1479-91. doi: 10.1016/j.cell.2013.12.001. Template for NLS-dCas9-NLS-EGFP fusion protein for CRISPR imaging (the recipient vector can be TetON 3G promoter system) pMSCVpuro
pBSKΔB-TRE-dCas9-EGFPdCas9-EGFP (Synthetic)TRE Ochiai Simultaneous live imaging of the transcription and nuclear position of specific genes. Nucleic Acids Res. 2015 Jun 19. pii: gkv624. Plasmid for tet-inducible expression of dCas9-EGFP in mammalian cells pBlueScript II SK (+)
pHAGE-EFS-dCas9-GFPSp dCas9 (Synthetic)EFS Pederson Multicolor CRISPR labeling of chromosomal loci in human cells. Proc Natl Acad Sci U S A. 2015 Mar 10;112(10):3002-7. doi: 10.1073/pnas.1420024112. Epub 2015 Feb 23. Expression of Sp dCas9-GFP in mammalian cells pHAGE-DEST
pHAGE-SSFV-dCas9-GFPSp dCas9 (Synthetic)SSFV Pederson Multicolor CRISPR labeling of chromosomal loci in human cells. Proc Natl Acad Sci U S A. 2015 Mar 10;112(10):3002-7. doi: 10.1073/pnas.1420024112. Epub 2015 Feb 23. Expression of Sp dCas9-GFP in mammalian cells pHAGE-DEST
pHAGE-TO-dCas9-GFPSp dCas9 (Synthetic)CMV-TO Pederson Multicolor CRISPR labeling of chromosomal loci in human cells. Proc Natl Acad Sci U S A. 2015 Mar 10;112(10):3002-7. doi: 10.1073/pnas.1420024112. Epub 2015 Feb 23. Expression of Sp dCas9-GFP in mammalian cells pHAGE-DEST
pHAGE-TO-dCas9-3XGFPSp dCas9 (Synthetic)CMV-TOGFP Pederson Multicolor CRISPR labeling of chromosomal loci in human cells. Proc Natl Acad Sci U S A. 2015 Mar 10;112(10):3002-7. doi: 10.1073/pnas.1420024112. Epub 2015 Feb 23. Expression of Sp dCas9-3XGFP in mammalian cells pHAGE-DEST
pHAGE-TO-dCas9-3XmCherrySp dCas9 (Synthetic)CMV-TOmCherry Pederson Multicolor CRISPR labeling of chromosomal loci in human cells. Proc Natl Acad Sci U S A. 2015 Mar 10;112(10):3002-7. doi: 10.1073/pnas.1420024112. Epub 2015 Feb 23. Expression of Sp dCas9-3XmCherry in mammalian cells pHAGE-DEST
pHAGE-TO-nmdCas9-3XGFPNm dCas9 (Synthetic)CMV-TO Pederson Multicolor CRISPR labeling of chromosomal loci in human cells. Proc Natl Acad Sci U S A. 2015 Mar 10;112(10):3002-7. doi: 10.1073/pnas.1420024112. Epub 2015 Feb 23. Expression of Nm dCas9-3XGFP in mammalian cells pHAGE-DEST
pHAGE-TO-nmdCas9-3XmCherryNm dCas9 (Synthetic)CMV-TO Pederson Multicolor CRISPR labeling of chromosomal loci in human cells. Proc Natl Acad Sci U S A. 2015 Mar 10;112(10):3002-7. doi: 10.1073/pnas.1420024112. Epub 2015 Feb 23. Expression of Nm dCas9-3XmCherry in mammalian cells pHAGE-DEST
pHAGE-TO-nls-st1dCas9-3nls-3XGFPSt1 dCas9 (Synthetic)CMV-TO Pederson Multicolor CRISPR labeling of chromosomal loci in human cells. Proc Natl Acad Sci U S A. 2015 Mar 10;112(10):3002-7. doi: 10.1073/pnas.1420024112. Epub 2015 Feb 23. Expression of St1 dCas9-3XGFP in mammalian cells pHAGE-DEST
pHAGE-TO-nls-st1dCas9-3nls-3XGFP-nlsSt1 dCas9 (Synthetic)CMV-TO Pederson Multicolor CRISPR labeling of chromosomal loci in human cells. Proc Natl Acad Sci U S A. 2015 Mar 10;112(10):3002-7. doi: 10.1073/pnas.1420024112. Epub 2015 Feb 23. Expression of St1 dCas9-3XGFP in mammalian cells pHAGE-DEST
pHAGE-TO-nls-st1dCas9-3nls-3XGFP-2nlsSt1 dCas9 (Synthetic)CMV-TO Pederson Multicolor CRISPR labeling of chromosomal loci in human cells. Proc Natl Acad Sci U S A. 2015 Mar 10;112(10):3002-7. doi: 10.1073/pnas.1420024112. Epub 2015 Feb 23. Expression of St1 dCas9-3XGFP in mammalian cells pHAGE-DEST
pHAGE-TO-nls-st1dCas9-3nls-3XTagBFP2St1 dCas9 (Synthetic)CMV-TO Pederson Multicolor CRISPR labeling of chromosomal loci in human cells. Proc Natl Acad Sci U S A. 2015 Mar 10;112(10):3002-7. doi: 10.1073/pnas.1420024112. Epub 2015 Feb 23. Expression of St1 dCas9-3XTagBFP2 in mammalian cells pHAGE-DEST
pCDNA3.1- dCas9-2xNLS-EGFPdCas9-2xNLS-EGFPCMV Yeo Programmable RNA Tracking in Live Cells with CRISPR/Cas9. Cell. 2016 Mar 16. pii: S0092-8674(16)30204-5. doi: 10.1016/j.cell.2016.02.054. Expresses dCas9-2xNLS-EGFP in mammalian cells pCDNA3.1(-)


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