| pHR-SFFV-dCas9-BFP | dCas9-BFP fusion (Homo sapiens) | SFFV | |
Weissman
| CRISPR-Mediated Modular RNA-Guided Regulation of Transcription in Eukaryotes.
Cell. 2013 Jul 9. pii: S0092-8674(13)00826-X. doi: 10.1016/j.cell.2013.06.044.
|
Human expression vector containing SFFV promoter, dCas9 that is fused to 2x NLS and tagBFP
pHR
|
| pSLQ1658-dCas9-EGFP | dCas9 fuse to EGFP (Homo sapiens) | MSCV LTR promoter | Puromycin |
Qi
| Dynamic Imaging of Genomic Loci in Living Human Cells by an Optimized CRISPR/Cas System.
Cell. 2013 Dec 19;155(7):1479-91. doi: 10.1016/j.cell.2013.12.001.
|
Template for NLS-dCas9-NLS-EGFP fusion protein for CRISPR imaging (the recipient vector can be TetON 3G promoter system)
pMSCVpuro
|
| pBSKΔB-TRE-dCas9-EGFP | dCas9-EGFP (Synthetic) | TRE | |
Ochiai
| Simultaneous live imaging of the transcription and nuclear position of specific genes.
Nucleic Acids Res. 2015 Jun 19. pii: gkv624.
|
Plasmid for tet-inducible expression of dCas9-EGFP in mammalian cells
pBlueScript II SK (+)
|
| pHAGE-EFS-dCas9-GFP | Sp dCas9 (Synthetic) | EFS | |
Pederson
| Multicolor CRISPR labeling of chromosomal loci in human cells.
Proc Natl Acad Sci U S A. 2015 Mar 10;112(10):3002-7. doi: 10.1073/pnas.1420024112. Epub 2015 Feb 23.
|
Expression of Sp dCas9-GFP in mammalian cells
pHAGE-DEST
|
| pHAGE-SSFV-dCas9-GFP | Sp dCas9 (Synthetic) | SSFV | |
Pederson
| Multicolor CRISPR labeling of chromosomal loci in human cells.
Proc Natl Acad Sci U S A. 2015 Mar 10;112(10):3002-7. doi: 10.1073/pnas.1420024112. Epub 2015 Feb 23.
|
Expression of Sp dCas9-GFP in mammalian cells
pHAGE-DEST
|
| pHAGE-TO-dCas9-GFP | Sp dCas9 (Synthetic) | CMV-TO | |
Pederson
| Multicolor CRISPR labeling of chromosomal loci in human cells.
Proc Natl Acad Sci U S A. 2015 Mar 10;112(10):3002-7. doi: 10.1073/pnas.1420024112. Epub 2015 Feb 23.
|
Expression of Sp dCas9-GFP in mammalian cells
pHAGE-DEST
|
| pHAGE-TO-dCas9-3XGFP | Sp dCas9 (Synthetic) | CMV-TO | GFP |
Pederson
| Multicolor CRISPR labeling of chromosomal loci in human cells.
Proc Natl Acad Sci U S A. 2015 Mar 10;112(10):3002-7. doi: 10.1073/pnas.1420024112. Epub 2015 Feb 23.
|
Expression of Sp dCas9-3XGFP in mammalian cells
pHAGE-DEST
|
| pHAGE-TO-dCas9-3XmCherry | Sp dCas9 (Synthetic) | CMV-TO | mCherry |
Pederson
| Multicolor CRISPR labeling of chromosomal loci in human cells.
Proc Natl Acad Sci U S A. 2015 Mar 10;112(10):3002-7. doi: 10.1073/pnas.1420024112. Epub 2015 Feb 23.
|
Expression of Sp dCas9-3XmCherry in mammalian cells
pHAGE-DEST
|
| pHAGE-TO-nmdCas9-3XGFP | Nm dCas9 (Synthetic) | CMV-TO | |
Pederson
| Multicolor CRISPR labeling of chromosomal loci in human cells.
Proc Natl Acad Sci U S A. 2015 Mar 10;112(10):3002-7. doi: 10.1073/pnas.1420024112. Epub 2015 Feb 23.
|
Expression of Nm dCas9-3XGFP in mammalian cells
pHAGE-DEST
|
| pHAGE-TO-nmdCas9-3XmCherry | Nm dCas9 (Synthetic) | CMV-TO | |
Pederson
| Multicolor CRISPR labeling of chromosomal loci in human cells.
Proc Natl Acad Sci U S A. 2015 Mar 10;112(10):3002-7. doi: 10.1073/pnas.1420024112. Epub 2015 Feb 23.
|
Expression of Nm dCas9-3XmCherry in mammalian cells
pHAGE-DEST
|
| pHAGE-TO-nls-st1dCas9-3nls-3XGFP | St1 dCas9 (Synthetic) | CMV-TO | |
Pederson
| Multicolor CRISPR labeling of chromosomal loci in human cells.
Proc Natl Acad Sci U S A. 2015 Mar 10;112(10):3002-7. doi: 10.1073/pnas.1420024112. Epub 2015 Feb 23.
|
Expression of St1 dCas9-3XGFP in mammalian cells
pHAGE-DEST
|
| pHAGE-TO-nls-st1dCas9-3nls-3XGFP-nls | St1 dCas9 (Synthetic) | CMV-TO | |
Pederson
| Multicolor CRISPR labeling of chromosomal loci in human cells.
Proc Natl Acad Sci U S A. 2015 Mar 10;112(10):3002-7. doi: 10.1073/pnas.1420024112. Epub 2015 Feb 23.
|
Expression of St1 dCas9-3XGFP in mammalian cells
pHAGE-DEST
|
| pHAGE-TO-nls-st1dCas9-3nls-3XGFP-2nls | St1 dCas9 (Synthetic) | CMV-TO | |
Pederson
| Multicolor CRISPR labeling of chromosomal loci in human cells.
Proc Natl Acad Sci U S A. 2015 Mar 10;112(10):3002-7. doi: 10.1073/pnas.1420024112. Epub 2015 Feb 23.
|
Expression of St1 dCas9-3XGFP in mammalian cells
pHAGE-DEST
|
| pHAGE-TO-nls-st1dCas9-3nls-3XTagBFP2 | St1 dCas9 (Synthetic) | CMV-TO | |
Pederson
| Multicolor CRISPR labeling of chromosomal loci in human cells.
Proc Natl Acad Sci U S A. 2015 Mar 10;112(10):3002-7. doi: 10.1073/pnas.1420024112. Epub 2015 Feb 23.
|
Expression of St1 dCas9-3XTagBFP2 in mammalian cells
pHAGE-DEST
|
| pCDNA3.1- dCas9-2xNLS-EGFP | dCas9-2xNLS-EGFP | CMV | |
Yeo
| Programmable RNA Tracking in Live Cells with CRISPR/Cas9.
Cell. 2016 Mar 16. pii: S0092-8674(16)30204-5. doi: 10.1016/j.cell.2016.02.054.
|
Expresses dCas9-2xNLS-EGFP in mammalian cells
pCDNA3.1(-)
|
| pHAGE-TO-MCP-3XBFPnls | MCP (Synthetic) | CMV-TO | |
Pederson
| Multiplexed labeling of genomic loci with dCas9 and engineered sgRNAs using CRISPRainbow.
Nat Biotechnol. 2016 Apr 18. doi: 10.1038/nbt.3526.
|
MCP-3XBFPnls
pHAGE-DEST
|
| pHAGE-EFS-MCP-3XBFPnls | MCP (Synthetic) | EFS | |
Pederson
| Multiplexed labeling of genomic loci with dCas9 and engineered sgRNAs using CRISPRainbow.
Nat Biotechnol. 2016 Apr 18. doi: 10.1038/nbt.3526.
|
MCP-3XBFPnls
pHAGE-DEST
|
| pHAGE-EFS-PCP-3XGFPnls | PCP (Synthetic) | EFS | |
Pederson
| Multiplexed labeling of genomic loci with dCas9 and engineered sgRNAs using CRISPRainbow.
Nat Biotechnol. 2016 Apr 18. doi: 10.1038/nbt.3526.
|
PCP-3XGFPnls
pHAGE-DEST
|
| pHAGE-EFS-PCP-3XNeon-nls | PCP (Synthetic) | EFS | |
Pederson
| Multiplexed labeling of genomic loci with dCas9 and engineered sgRNAs using CRISPRainbow.
Nat Biotechnol. 2016 Apr 18. doi: 10.1038/nbt.3526.
|
PCP-3XNeon-nls
pHAGE-DEST
|
| pHAGE-EFS-N22p-3XRFPnls | N22p (Synthetic) | EFS | |
Pederson
| Multiplexed labeling of genomic loci with dCas9 and engineered sgRNAs using CRISPRainbow.
Nat Biotechnol. 2016 Apr 18. doi: 10.1038/nbt.3526.
|
N22p-3XRFPnls
pHAGE-DEST
|
| pLH-sgRNA1-2XMS2 | sgRNA1-2XMS2 (Synthetic) | U6 | Hygromycin |
Pederson
| Multiplexed labeling of genomic loci with dCas9 and engineered sgRNAs using CRISPRainbow.
Nat Biotechnol. 2016 Apr 18. doi: 10.1038/nbt.3526.
|
sgRNA1-2XMS2
pLKO.1
|
| pLH-sgRNA1-2XPP7 | sgRNA1-2XPP7 (Synthetic) | U6 | Hygromycin |
Pederson
| Multiplexed labeling of genomic loci with dCas9 and engineered sgRNAs using CRISPRainbow.
Nat Biotechnol. 2016 Apr 18. doi: 10.1038/nbt.3526.
|
sgRNA1-2XPP7
pLKO.1
|
| pLH-sgRNA1-2XboxB | sgRNA1-2XboxB (Synthetic) | U6 | Hygromycin |
Pederson
| Multiplexed labeling of genomic loci with dCas9 and engineered sgRNAs using CRISPRainbow.
Nat Biotechnol. 2016 Apr 18. doi: 10.1038/nbt.3526.
|
sgRNA1-2XboxB
pLKO.1
|
| pLH-sgRNA1-MS2-PP7 | sgRNA1-2XMS2-PP7 (Synthetic) | U6 | Hygromycin |
Pederson
| Multiplexed labeling of genomic loci with dCas9 and engineered sgRNAs using CRISPRainbow.
Nat Biotechnol. 2016 Apr 18. doi: 10.1038/nbt.3526.
|
sgRNA1-MS2-PP7
pLKO.1
|
| pLH-sgRNA1-PP7-boxB | sgRNA1-2XPP7-boxB (Synthetic) | U6 | |
Pederson
| Multiplexed labeling of genomic loci with dCas9 and engineered sgRNAs using CRISPRainbow.
Nat Biotechnol. 2016 Apr 18. doi: 10.1038/nbt.3526.
|
sgRNA1-PP7-boxB
pLKO.1
|
| pLH-sgRNA1-boxB-MS2 | sgRNA1-boxB-MS2 (Synthetic) | U6 | Hygromycin |
Pederson
| Multiplexed labeling of genomic loci with dCas9 and engineered sgRNAs using CRISPRainbow.
Nat Biotechnol. 2016 Apr 18. doi: 10.1038/nbt.3526.
|
sgRNA1-boxB-MS2
pLKO.1
|
| pLH-sgRNA1-boxB-MS2-PP7 | sgRNA1-boxB-MS2-PP7 (Synthetic) | U6 | Hygromycin |
Pederson
| Multiplexed labeling of genomic loci with dCas9 and engineered sgRNAs using CRISPRainbow.
Nat Biotechnol. 2016 Apr 18. doi: 10.1038/nbt.3526.
|
sgRNA1-boxB-MS2-PP7
pLKO.1
|
| pHAGE-TO-dCas9-P2A-HSA | dCas9-P2A-HSA (Synthetic) | CMV-TO | |
Pederson
| CRISPR-Sirius: RNA scaffolds for signal amplification in genome imaging.
Nat Methods. 2018 Nov;15(11):928-931. doi: 10.1038/s41592-018-0174-0. Epub 2018 Oct 30.
|
CRISPR-Sirius plasmid
pHAGE-TO
|
| pHAGE-EFS-MCP-HALOnls | MCP-HALOnls (Synthetic) | EFS | |
Pederson
| CRISPR-Sirius: RNA scaffolds for signal amplification in genome imaging.
Nat Methods. 2018 Nov;15(11):928-931. doi: 10.1038/s41592-018-0174-0. Epub 2018 Oct 30.
|
CRISPR-Sirius plasmid
pHAGE-EFS
|
| pHAGE-EFS-PCP-GFPnls | PCP-GFPnls (Synthetic) | EFS | |
Pederson
| CRISPR-Sirius: RNA scaffolds for signal amplification in genome imaging.
Nat Methods. 2018 Nov;15(11):928-931. doi: 10.1038/s41592-018-0174-0. Epub 2018 Oct 30.
|
CRISPR-Sirius plasmid
pHAGE-EFS
|
| pLH-sgRNA-Sirius-8XMS2 | sgRNA-Sirius-8XMS2 (Synthetic) | human U6 promoter | Hygromycin |
Pederson
| CRISPR-Sirius: RNA scaffolds for signal amplification in genome imaging.
Nat Methods. 2018 Nov;15(11):928-931. doi: 10.1038/s41592-018-0174-0. Epub 2018 Oct 30.
|
CRISPR-Sirius plasmid
pLKO.1
|
| pLH-sgRNA-Sirius-8XPP7 | sgRNA-Sirius-8XPP7 (Synthetic) | human U6 promoter | Hygromycin |
Pederson
| CRISPR-Sirius: RNA scaffolds for signal amplification in genome imaging.
Nat Methods. 2018 Nov;15(11):928-931. doi: 10.1038/s41592-018-0174-0. Epub 2018 Oct 30.
|
CRISPR-Sirius plasmid
pLKO.1
|
| pLH-sgRNA-Sirius-4X(MS2-PP7) | sgRNA-Sirius-4X(MS2-PP7) (Synthetic) | human U6 promoter | Hygromycin |
Pederson
| CRISPR-Sirius: RNA scaffolds for signal amplification in genome imaging.
Nat Methods. 2018 Nov;15(11):928-931. doi: 10.1038/s41592-018-0174-0. Epub 2018 Oct 30.
|
CRISPR-Sirius plasmid
pLKO.1
|
| pPUR-hU6-sgRNA-Sirius-8XMS2 | sgRNA-Sirius-8XMS2 (Synthetic) | human U6 promoter | Puromycin |
Pederson
| CRISPR-Sirius: RNA scaffolds for signal amplification in genome imaging.
Nat Methods. 2018 Nov;15(11):928-931. doi: 10.1038/s41592-018-0174-0. Epub 2018 Oct 30.
|
CRISPR-Sirius plasmid
pLKO.1
|
| pPUR-mU6-sgRNA-Sirius-8XPP7 | sgRNA-Sirius-8XPP7 (Synthetic) | mouse U6 promoter | Puromycin |
Pederson
| CRISPR-Sirius: RNA scaffolds for signal amplification in genome imaging.
Nat Methods. 2018 Nov;15(11):928-931. doi: 10.1038/s41592-018-0174-0. Epub 2018 Oct 30.
|
CRISPR-Sirius plasmid
pLKO.1
|
| pPUR-hU6-Sirius-8XMS2-mU6-Sirius-8XPP7 | hU6-Sirius-8XMS2-mU6-Sirius-8XPP7 Cassette (Synthetic) | U6 promoters | Puromycin |
Pederson
| CRISPR-Sirius: RNA scaffolds for signal amplification in genome imaging.
Nat Methods. 2018 Nov;15(11):928-931. doi: 10.1038/s41592-018-0174-0. Epub 2018 Oct 30.
|
CRISPR-Sirius plasmid
pLKO.1
|