PurposeExpression of customizable guide RNA (gRNA) under cotnrol of murine U6 promoter, also contains a CMV-puro-t2A-mCherry expression cassette for mammalian gene knockdown
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||44248||Plasmid sent as bacteria in agar stab||1||$65|
This material is available to academics and nonprofits only.
- Total vector size (bp) 8308
Vector typeMammalian Expression, Lentiviral, CRISPR
Growth in Bacteria
Growth Strain(s)NEB Stable
Copy numberHigh Copy
Gene/Insert nameguide RNA
SpeciesH. sapiens (human)
Insert Size (bp)102
- Promoter mouse U6
- Cloning method Restriction Enzyme
- 5′ cloning site BstXI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer n/a
- 3′ sequencing primer n/a (Common Sequencing Primers)
For more information on Qi Lab CRISPR Plasmids please refer to: http://www.addgene.org/crispr/qi/
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pgRNA-humanized was a gift from Stanley Qi (Addgene plasmid # 44248)
For your References section:Repurposing CRISPR as an RNA-Guided Platform for Sequence-Specific Control of Gene Expression. Qi LS, Larson MH, Gilbert LA, Doudna JA, Weissman JS, Arkin AP, Lim WA. Cell. 2013 Feb 28;152(5):1173-83. doi: 10.1016/j.cell.2013.02.022. 10.1016/j.cell.2013.02.022 PubMed 23452860
Generated by Addgene from full sequence supplied by depositor.
Map uploaded by the depositor.