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Repurposing CRISPR as an RNA-Guided Platform for Sequence-Specific Control of Gene Expression.
Qi LS, Larson MH, Gilbert LA, Doudna JA, Weissman JS, Arkin AP, Lim WA
Cell. 2013 Feb 28;152(5):1173-83. doi: 10.1016/j.cell.2013.02.022.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
44246pdCas9-humanizedExpression of a catalytically inactive, human codon-optimized Cas9 under the control of Murine Stem Cell retroVirus LTR promoter for mammalian gene knockdown
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44247pdCas9::BFP-humanizedExpression of a catalytically inactive, human codon-optimized Cas9-BFP fusion under the control of Murine Stem Cell retroVirus LTR promoter for mammalian gene knockdown
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44248pgRNA-humanizedExpression of customizable guide RNA (gRNA) under cotnrol of murine U6 promoter, also contains a CMV-puro-t2A-mCherry expression cassette for mammalian gene knockdown
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44249pdCas9-bacteriaaTc-inducible expression of a catalytically inactive bacterial Cas9 (S. pyogenes) for bacterial gene knockdown
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44250pwtCas9-bacteriaaTc-inducible expression of wild-type Cas9 (S .pyogenes) for bacterial gene knockdown
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44251pgRNA-bacteriaExpression of customizable guide RNA (gRNA) for bacterial gene knockdown
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