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pwtCas9-bacteria
(Plasmid #44250)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 44250 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Top10
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    wild-type Cas9
  • Species
    S .pyogenes
  • Insert Size (bp)
    4107
  • Promoter pLtetO-1

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BglII (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer agaaaagaattcaaaAGATCTaaagaggagaaaGGATCTATGGATAAGAAATACTCAATAGG
  • 3′ sequencing primer ggagatccttactcgagttaGTCACCTCCTAGCTGACTCAAATCAAT
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

For more information on Qi Lab CRISPR Plasmids please refer to: http://www.addgene.org/crispr/qi/

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pwtCas9-bacteria was a gift from Stanley Qi (Addgene plasmid # 44250)
  • For your References section:

    Repurposing CRISPR as an RNA-Guided Platform for Sequence-Specific Control of Gene Expression. Qi LS, Larson MH, Gilbert LA, Doudna JA, Weissman JS, Arkin AP, Lim WA. Cell. 2013 Feb 28;152(5):1173-83. doi: 10.1016/j.cell.2013.02.022. 10.1016/j.cell.2013.02.022 PubMed 23452860