PurposeBacterial expression of Cas9 nuclease, tracrRNA and crRNA guide
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||42876||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4244
- Total vector size (bp) 9326
Vector typeBacterial Expression, CRISPR ; E.coli
Growth in Bacteria
Growth instructionspCas9 is resistant to chloramphenicol (25ug/ml).
Copy numberLow Copy
The HME63 strain is available from the from the Court Lab:
For more information on Marraffini Lab CRISPR Plasmids please refer to: http://www.addgene.org/crispr/marraffini/
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCas9 was a gift from Luciano Marraffini (Addgene plasmid # 42876 ; http://n2t.net/addgene:42876 ; RRID:Addgene_42876)
For your References section:RNA-guided editing of bacterial genomes using CRISPR-Cas systems. Jiang W, Bikard D, Cox D, Zhang F, Marraffini LA. Nat Biotechnol. 2013 Jan 29. doi: 10.1038/nbt.2508. 10.1038/nbt.2508 PubMed 23360965