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pMSCV-LTR-dCas9-VP64-BFP
(Plasmid #46912)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 46912 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    MSCV-puro
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 6300
  • Total vector size (bp) 11369
  • Vector type
    Mammalian Expression, Retroviral, CRISPR
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    Low Copy

Gene/Insert 1

  • Gene/Insert name
    dCas9-VP64-BFP fusion
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    5055
  • Promoter LTR
  • Tags / Fusion Proteins
    • 3xNLS (C terminal on insert)
    • BFP (C terminal on insert)
    • VP64 domain (C terminal on insert)

Cloning Information for Gene/Insert 1

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer CCCTTGAACCTCCTCGTTCGACC
  • 3′ sequencing primer GAGACGTGCTACTTCCATTTGTC
  • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    Puromycin resistance
  • Insert Size (bp)
    654
  • Promoter PGK

Cloning Information for Gene/Insert 2

Resource Information

Depositor Comments

The Cas9 contains a N612K mutation that does not effect the function of the enzyme.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMSCV-LTR-dCas9-VP64-BFP was a gift from Stanley Qi & Jonathan Weissman (Addgene plasmid # 46912)
  • For your References section:

    CRISPR-Mediated Modular RNA-Guided Regulation of Transcription in Eukaryotes. Gilbert LA, Larson MH, Morsut L, Liu Z, Brar GA, Torres SE, Stern-Ginossar N, Brandman O, Whitehead EH, Doudna JA, Lim WA, Weissman JS, Qi LS. Cell. 2013 Jul 9. pii: S0092-8674(13)00826-X. doi: 10.1016/j.cell.2013.06.044. 10.1016/j.cell.2013.06.044 PubMed 23849981