PurposeDual expression construct expressing both dCas9VP160 and sgRNA from separate promoters
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||48240||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Vector backbonepX335 (Addgene #42335)
Vector typeMammalian Expression, CRISPR
Growth in Bacteria
/ Fusion Proteins
- HA-tag (N terminal on insert)
- VP160 (C terminal on insert)
Clone sgRNA spacer into BbsI site. Sequence using LKO5' primer: GACTATCATATGCTTACCGT
For more information including protocols and
updates, please go to http://www.crispr-on.org
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pAC154-dual-dCas9VP160-sgExpression was a gift from Rudolf Jaenisch (Addgene plasmid # 48240 ; http://n2t.net/addgene:48240 ; RRID:Addgene_48240)
For your References section:Multiplexed activation of endogenous genes by CRISPR-on, an RNA-guided transcriptional activator system. Cheng AW, Wang H, Yang H, Shi L, Katz Y, Theunissen TW, Rangarajan S, Shivalila CS, Dadon DB, Jaenisch R. Cell Res. 2013 Aug 27. doi: 10.1038/cr.2013.122. 10.1038/cr.2013.122 PubMed 23979020