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Multiplexed activation of endogenous genes by CRISPR-on, an RNA-guided transcriptional activator system.
Cheng AW, Wang H, Yang H, Shi L, Katz Y, Theunissen TW, Rangarajan S, Shivalila CS, Dadon DB, Jaenisch R
Cell Res. 2013 Aug 27. doi: 10.1038/cr.2013.122. PubMed Article

Plasmids from Article

ID Plasmid Purpose
48214pAC1-pCR8-dCas9VP48dCas9VP48 on Gateway donor vector pCR8/GW/TOPO. Note: This is not for expression. It has to be transferred to a gateway destination vector for expression
48218pAC84-pCR8-dCas9dCas9 on Gateway donor vector pCR8/GW/TOPO. Note: This is not for expression. It has to be transferred to a gateway destination vector for expression
48219pAC147-pCR8-dCas9VP64dCas9VP64 on Gateway donor vector pCR8/GW/TOPO. Note: This is not for expression. It has to be transferred to a gateway destination vector for expression 
48220pAC148-pCR8-dCas9VP96dCas9VP96 on Gateway donor vector pCR8/GW/TOPO. Note: This is not for expression. It has to be transferred to a gateway destination vector for expression
48221pAC149-pCR8-dCas9VP160dCas9VP160 on Gateway donor vector pCR8/GW/TOPO. Note: This is not for expression. It has to be transferred to a gateway destination vector for expression
48222pAC90-pmax-DESTGateway Destination vector derived from Clontech's pmax (CAGGS) expression vector
48223pAC91-pmax-dCas9VP64dCas9VP64 on pmax expression vector
48224pAC92-pmax-dCas9VP96dCas9VP96 on pmax expression vector
48225pAC93-pmax-dCas9VP160dCas9VP160 on pmax expression vector
48226pAC94-pmax-dCas9VP160-2A-purodCas9VP160-2A-puro (puro-selectable) on pmax expression vecor. Note: This is being tested.
48227pAC95-pmax-dCas9VP160-2A-neodCas9VP160-2A-neo (neo/G418-selectable) on pmax expression vector. Note: This is being tested.
48228pAC103-PBNeo-U6-sgRNA-ExpressionsgRNA expression on PiggyBac vector with Neo-selectable marker
48234pAC150-PBLHL-4xHS-EF1a-DESTPiggyBac expression gateway destination vector with Insulator-flanked EF1a promoter gateway cassette and floxed Hygro selection marker
48236pAC2-dual-dCas9VP48-sgExpressionDual expression construct expressing both dCas9VP48 and sgRNA from separate promoters
48237pAC5-dual-dCas9VP48-sgTetODual expression construct expressing both dCas9VP48 and sgTetO from separate promoters
48238pAC152-dual-dCas9VP64-sgExpressionDual expression construct expressing both dCas9VP64 and sgRNA from separate promoters
48239pAC153-dual-dCas9VP96-sgExpressionDual expression construct expressing both dCas9VP96 and sgRNA from separate promoters
48240pAC154-dual-dCas9VP160-sgExpressionDual expression construct expressing both dCas9VP160 and sgRNA from separate promoters
49045pAC155-pCR8-sgExpressionU6-driven sgRNA expressing cassette on a gateway donor vector

Antibodies from Article