PurposedCas9VP160-2A-puro (puro-selectable) on pmax expression vecor. Note: This is being tested.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||48226||Plasmid sent as bacteria in agar stab||1||$65|
This material is available to academics and nonprofits only.
Vector backbonepmax-DEST (Addgene: 48222)
Vector typeMammalian Expression, CRISPR
Growth in Bacteria
Gene/Insert namedCas9(D10A;H840A) fusion with VP160 activation domain followed by 2A-puro
SpeciesH. sapiens (human), Synthetic
Insert Size (bp)5528
MutationD10A;H840A (catalytically inactive)
- Promoter CAGGS
/ Fusion Proteins
- HA Tag (N terminal on insert)
- VP160 (C terminal on insert)
- 2A-puro (C terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site ClaI (not destroyed)
- 3′ cloning site PacI (not destroyed)
- 5′ sequencing primer gggcttgtcgagacagagaagat
- 3′ sequencing primer ACCGAGGAGAGGGTTAGGGAT (Common Sequencing Primers)
For more information including protocols and
updates, please go to http://www.crispr-on.org
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pAC94-pmax-dCas9VP160-2A-puro was a gift from Rudolf Jaenisch (Addgene plasmid # 48226)
For your References section:Multiplexed activation of endogenous genes by CRISPR-on, an RNA-guided transcriptional activator system. Cheng AW, Wang H, Yang H, Shi L, Katz Y, Theunissen TW, Rangarajan S, Shivalila CS, Dadon DB, Jaenisch R. Cell Res. 2013 Aug 27. doi: 10.1038/cr.2013.122. 10.1038/cr.2013.122 PubMed 23979020
Generated by Addgene from full sequence supplied by depositor.