PurposeDual expression construct expressing both dCas9VP48 and sgRNA from separate promoters
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||48236||Plasmid sent as bacteria in agar stab||1||$65|
This material is available to academics and nonprofits only.
Vector backbonepX335 (Addgene #42335)
Vector typeMammalian Expression, CRISPR
Growth in Bacteria
SpeciesH. sapiens (human), Synthetic
MutationD10A H840A (catalytically inactive)
/ Fusion Proteins
- HA-tag (N terminal on insert)
- VP48 (C terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site AgeI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer acctctccgcctcgccccgc
- 3′ sequencing primer BGHPolyARSeq:ttattaggaaaggacagtgg (Common Sequencing Primers)
Clone sgRNA spacer into BbsI site. Sequence using LKO5' primer: GACTATCATATGCTTACCGT
For more information including protocols and
updates, please go to http://www.crispr-on.org
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pAC2-dual-dCas9VP48-sgExpression was a gift from Rudolf Jaenisch (Addgene plasmid # 48236)
For your References section:Multiplexed activation of endogenous genes by CRISPR-on, an RNA-guided transcriptional activator system. Cheng AW, Wang H, Yang H, Shi L, Katz Y, Theunissen TW, Rangarajan S, Shivalila CS, Dadon DB, Jaenisch R. Cell Res. 2013 Aug 27. doi: 10.1038/cr.2013.122. 10.1038/cr.2013.122 PubMed 23979020
Generated by Addgene from full sequence supplied by depositor.