Purpose(Empty Backbone) Gateway Destination vector derived from Clontech's pmax (CAGGS) expression vector
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||48222||Plasmid sent as bacteria in agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size (bp) 2786
Vector typeMammalian Expression, CRISPR ; Gateway Destination
- Promoter CAGGS
Growth in Bacteria
Bacterial Resistance(s)Chloramphenicol and Kanamycin
Growth Strain(s)ccdB Survival
- Cloning method Restriction Enzyme
- 5′ sequencing primer gggcttgtcgagacagagaagat (Common Sequencing Primers)
For more information including protocols and
updates, please go to http://www.crispr-on.org
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pAC90-pmax-DEST was a gift from Rudolf Jaenisch (Addgene plasmid # 48222)
For your References section:Multiplexed activation of endogenous genes by CRISPR-on, an RNA-guided transcriptional activator system. Cheng AW, Wang H, Yang H, Shi L, Katz Y, Theunissen TW, Rangarajan S, Shivalila CS, Dadon DB, Jaenisch R. Cell Res. 2013 Aug 27. doi: 10.1038/cr.2013.122. 10.1038/cr.2013.122 PubMed 23979020
Generated by Addgene from full sequence supplied by depositor.