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CRISPR: dCas9-FokI


A catalytically inactive Cas9 (dCas9) is fused to FokI nuclease. When FokI dimerizes, it generates a double-strand break (DSB) at a specific sequence. Two unique gRNAs, binding ~15-25 bp apart, are required for dCas9-FokI to dimerize at a given region of the genome. Like nickase technology, this technique reduces unwanted off-target effects of CRISPR.



Browse, sort, or search the tables below for CRISPR-FokI plasmids.
Plasmids are available for expression in mammalian systems and Drosophila.


Mammalian

ID Plasmid Gene/Insert Promoter PI Publication

Drosophila

ID Plasmid Gene/Insert Promoter PI Publication

Yeast

ID Plasmid Gene/Insert Promoter PI Publication


Do you have suggestions for other plasmids that should be added to this list?

Fill out our Suggest a Plasmid form or e-mail [email protected] to help us improve this resource!