Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Addgene is open for ordering and depositing; find up-to-date details here. To learn more about how we are supporting COVID-19 research and to find related plasmids, check out our COVID-19 and Coronavirus Plasmids & Resources page.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

Joung_CRISPR-Cas.jpg Joung Lab CRISPR-Cas/RNA-Guided Nuclease (RGN) Plasmids

RNA-Guided Nucleases ( RGNs ), based on naturally occurring Type II CRISPR-Cas systems, are programmable endonucleases that can be used to perform targeted genome editing. RGNs consist of two components: a short ~100 nt guide RNA ( gRNA ) that uses 20 variable nts at its 5’ end to base pair with a target genomic DNA sequence and a nuclease (e.g.—the Cas9 endonuclease) that cleaves the target DNA.

The Joung lab recently described gRNA and Cas9 expression vectors and validated the use of RNAs produced by these vectors for efficiently modifying endogenous genes in zebrafish (Hwang and Fu et al., Nat Biotechnol. 2013).

The Joung lab has also modified their web-based ZiFiT Targeter software to enable identification of potential RGN target sites and to facilitate design of short oligonucleotides needed to construct gRNA expression vectors. More information about RGNs and CRISPR-Cas systems can also be found at . Users are also encouraged to join the Genome Engineering newsgroup here .

Links to additional pages describing Joung Lab CRISPR-Cas/RGN reagents: