FokI-dCas9
(Plasmid #52970)

• Purpose: Expresses Fok1 nuclease domain fused to catalytically inactive Cas9 DNA-binding domain in mammalian cells
• Depositing Lab: David Liu
• Publication: Guilinger et al Nat Biotechnol. 2014 Apr 25. doi: 10.1038/nbt.2909.

Backbone

• Vector backbone: JDS246
• Backbone size w/o insert (bp): 3400
• Total vector size (bp): 8271
• Vector type: Mammalian Expression, CRISPR

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): XL1 Blue
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Fok1 fused to dCas9
• Insert Size (bp): 4800
• Mutation: D10A and H840A in Cas9
• Promoter: CMV
• Tags / Fusion Proteins:
  • NLS (N terminal on insert)
  • Fok1 nuclease (homodimeric wildtype) (N terminal on insert)

Cloning Information

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: TAATACGACTCACTATAGG
• 3′ sequencing primer: CAGGGTCAAGGAAGGCACG

Resource Information

• Articles Citing this Plasmid:
  • 7 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry