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FokI-dCas9
(Plasmid #52970)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 52970 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    JDS246
  • Backbone size w/o insert (bp) 3400
  • Total vector size (bp) 8271
  • Vector type
    Mammalian Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    XL1 Blue
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Fok1 fused to dCas9
  • Insert Size (bp)
    4800
  • Mutation
    D10A and H840A in Cas9
  • Promoter CMV
  • Tags / Fusion Proteins
    • NLS (N terminal on insert)
    • Fok1 nuclease (homodimeric wildtype) (N terminal on insert)

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer TAATACGACTCACTATAGG
  • 3′ sequencing primer CAGGGTCAAGGAAGGCACG
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    FokI-dCas9 was a gift from David Liu (Addgene plasmid # 52970 ; http://n2t.net/addgene:52970 ; RRID:Addgene_52970)
  • For your References section:

    Fusion of catalytically inactive Cas9 to FokI nuclease improves the specificity of genome modification. Guilinger JP, Thompson DB, Liu DR. Nat Biotechnol. 2014 Apr 25. doi: 10.1038/nbt.2909. 10.1038/nbt.2909 PubMed 24770324