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Feng Zhang Lab Plasmids


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Title Authors Publication
Genome-scale transcriptional activation by an engineered CRISPR-Cas9 complex. Konermann S, Brigham MD, Trevino AE, Joung J, Abudayyeh OO, Barcena C, Hsu PD, Habib N, Gootenberg JS, Nishimasu H, Nureki O, Zhang F Nature. 2014 Dec 10. doi: 10.1038/nature14136.
In vivo interrogation of gene function in the mammalian brain using CRISPR-Cas9. Swiech L, Heidenreich M, Banerjee A, Habib N, Li Y, Trombetta J, Sur M, Zhang F Nat Biotechnol. 2014 Oct 19. doi: 10.1038/nbt.3055.
CRISPR-Cas9 Knockin Mice for Genome Editing and Cancer Modeling. Platt RJ, Chen S, Zhou Y, Yim MJ, Swiech L, Kempton HR, Dahlman JE, Parnas O, Eisenhaure TM, Jovanovic M, Graham DB, Jhunjhunwala S, Heidenreich M, Xavier RJ, Langer R, Anderson DG, Hacohen N, Regev A, Feng G, Sharp PA, Zhang F Cell. 2014 Sep 24. pii: S0092-8674(14)01163-5. doi: 10.1016/j.cell.2014.09.014.
Improved vectors and genome-wide libraries for CRISPR screening. Sanjana NE, Shalem O, Zhang F Nat Methods. 2014 Aug;11(8):783-4. doi: 10.1038/nmeth.3047.
Crystal structure of Cas9 in complex with guide RNA and target DNA. Nishimasu H, Ran FA, Hsu PD, Konermann S, Shehata SI, Dohmae N, Ishitani R, Zhang F, Nureki O Cell. 2014 Feb 27;156(5):935-49. doi: 10.1016/j.cell.2014.02.001. Epub 2014 Feb 13.
Genome-scale CRISPR-Cas9 knockout screening in human cells. Shalem O, Sanjana NE, Hartenian E, Shi X, Scott DA, Mikkelsen TS, Heckl D, Ebert BL, Root DE, Doench JG, Zhang F Science. 2014 Jan 3;343(6166):84-7. doi: 10.1126/science.1247005. Epub 2013 Dec 12.
Genome engineering using the CRISPR-Cas9 system. Ran FA, Hsu PD, Wright J, Agarwala V, Scott DA, Zhang F Nat Protoc. 2013 Nov;8(11):2281-308. doi: 10.1038/nprot.2013.143. Epub 2013 Oct 24.
Optical control of mammalian endogenous transcription and epigenetic states. Konermann S, Brigham MD, Trevino A, Hsu PD, Heidenreich M, Le Cong, Platt RJ, Scott DA, Church GM, Zhang F Nature. 2013 Jul 23. doi: 10.1038/nature12466.
Multiplex Genome Engineering Using CRISPR/Cas Systems. Cong L, Ran FA, Cox D, Lin S, Barretto R, Habib N, Hsu PD, Wu X, Jiang W, Marraffini LA, Zhang F Science. 2013 Jan 3.
Comprehensive interrogation of natural TALE DNA-binding modules and transcriptional repressor domains. Cong L, Zhou R, Kuo YC, Cunniff M, Zhang F Nat Commun. 2012 Jul 24;3:968. doi: 10.1038/ncomms1962.
A transcription activator-like effector toolbox for genome engineering. Sanjana NE, Cong L, Zhou Y, Cunniff MM, Feng G, Zhang F Nat Protoc. 2012 Jan 5;7(1):171-92. doi: 10.1038/nprot.2011.431.
Efficient construction of sequence-specific TAL effectors for modulating mammalian transcription. Zhang F, Cong L, Lodato S, Kosuri S, Church GM, Arlotta P Nat Biotechnol. 2011 Jan 19. ():.
TALE Nuclease (TALEN) (unpublished)
Plasmid Gene/Insert Vector Type Publication Hidden Extra Search Info  
pLenti-EF1a-Backbone(NN) TAL-Backbone(NN) (Synthetic) Mammalian Expression, Lentiviral Efficient construction of sequence-specific TAL effectors for modulating mammalian transcription. Zhang et al Nat Biotechnol. 2011 Jan 19. ():. pLenti Add to Cart
pLenti-EF1a-Backbone(NI) TAL-Backbone(NI) (Synthetic) Mammalian Expression, Lentiviral Efficient construction of sequence-specific TAL effectors for modulating mammalian transcription. Zhang et al Nat Biotechnol. 2011 Jan 19. ():. pLenti Add to Cart
pLenti-EF1a-Backbone(NG) TAL-Backbone(NG) (Synthetic) Mammalian Expression, Lentiviral Efficient construction of sequence-specific TAL effectors for modulating mammalian transcription. Zhang et al Nat Biotechnol. 2011 Jan 19. ():. pLenti Add to Cart
pLenti-EF1a-Backbone(HD) TAL-Backbone(HD) (Synthetic) Mammalian Expression, Lentiviral Efficient construction of sequence-specific TAL effectors for modulating mammalian transcription. Zhang et al Nat Biotechnol. 2011 Jan 19. ():. pLenti Add to Cart
monomer-NN TAL-monomer-NN Bacterial Expression Efficient construction of sequence-specific TAL effectors for modulating mammalian transcription. Zhang et al Nat Biotechnol. 2011 Jan 19. ():. pJ201 Add to Cart
monomer-NI TAL-monomer-NI Bacterial Expression Efficient construction of sequence-specific TAL effectors for modulating mammalian transcription. Zhang et al Nat Biotechnol. 2011 Jan 19. ():. pJ201 Add to Cart
monomer-NG TAL-monomer-NG Bacterial Expression Efficient construction of sequence-specific TAL effectors for modulating mammalian transcription. Zhang et al Nat Biotechnol. 2011 Jan 19. ():. pJ201 Add to Cart
monomer-HD TAL-monomer-HD Bacterial Expression Efficient construction of sequence-specific TAL effectors for modulating mammalian transcription. Zhang et al Nat Biotechnol. 2011 Jan 19. ():. pJ201 Add to Cart
pLenti-EF1a-TALE1 TALE1 (Synthetic) Mammalian Expression, Lentiviral Efficient construction of sequence-specific TAL effectors for modulating mammalian transcription. Zhang et al Nat Biotechnol. 2011 Jan 19. ():. pLenti Add to Cart
pAAV-minCMV-mCherry mCherry (Synthetic) Mammalian Expression Efficient construction of sequence-specific TAL effectors for modulating mammalian transcription. Zhang et al Nat Biotechnol. 2011 Jan 19. ():. pAAV Add to Cart
Monomer NK Monomer-NK Bacterial Expression TALE Nuclease (TALEN) (unpublished) pJ201 Add to Cart
TALEN Backbone TALE Nuclease Backbone Mammalian Expression TALE Nuclease (TALEN) (unpublished) pCMV Add to Cart
pNI_v2 TALE Monomer NI Bacterial Expression A transcription activator-like effector toolbox for genome engineering. Sanjana et al Nat Protoc. 2012 Jan 5;7(1):171-92. doi: 10.1038/nprot.2011.431. pJ201 Add to Cart
pNG_v2 TALE Monomer NG Bacterial Expression A transcription activator-like effector toolbox for genome engineering. Sanjana et al Nat Protoc. 2012 Jan 5;7(1):171-92. doi: 10.1038/nprot.2011.431. pJ201 Add to Cart
pNN_v2 TALE Monomer NN Bacterial Expression A transcription activator-like effector toolbox for genome engineering. Sanjana et al Nat Protoc. 2012 Jan 5;7(1):171-92. doi: 10.1038/nprot.2011.431. pJ201 Add to Cart
pHD_v2 TALE Monomer HD Bacterial Expression A transcription activator-like effector toolbox for genome engineering. Sanjana et al Nat Protoc. 2012 Jan 5;7(1):171-92. doi: 10.1038/nprot.2011.431. pJ201 Add to Cart
pTALETF_v2 (NN) TALE Transcriptional Activator Backbone, NN for 0.5 repeat Mammalian Expression A transcription activator-like effector toolbox for genome engineering. Sanjana et al Nat Protoc. 2012 Jan 5;7(1):171-92. doi: 10.1038/nprot.2011.431. pCMV Add to Cart
pTALETF_v2 (NG) TALE Transcriptional Activator Backbone, NG for 0.5 repeat Mammalian Expression A transcription activator-like effector toolbox for genome engineering. Sanjana et al Nat Protoc. 2012 Jan 5;7(1):171-92. doi: 10.1038/nprot.2011.431. pCMV Add to Cart
pTALETF_v2 (NI) TALE Transcriptional Activator Backbone, NI for 0.5 repeat Mammalian Expression A transcription activator-like effector toolbox for genome engineering. Sanjana et al Nat Protoc. 2012 Jan 5;7(1):171-92. doi: 10.1038/nprot.2011.431. pCMV Add to Cart
pTALETF_v2 (HD) TALE Transcriptional Activator Backbone, HD for 0.5 repeat Mammalian Expression A transcription activator-like effector toolbox for genome engineering. Sanjana et al Nat Protoc. 2012 Jan 5;7(1):171-92. doi: 10.1038/nprot.2011.431. pCMV Add to Cart
pTALEN_v2 (NI) TALE Nuclease, NI for 0.5 repeat Mammalian Expression A transcription activator-like effector toolbox for genome engineering. Sanjana et al Nat Protoc. 2012 Jan 5;7(1):171-92. doi: 10.1038/nprot.2011.431. pCMV Add to Cart
pTALEN_v2 (NG) TALE Nuclease, NG for 0.5 repeat Mammalian Expression A transcription activator-like effector toolbox for genome engineering. Sanjana et al Nat Protoc. 2012 Jan 5;7(1):171-92. doi: 10.1038/nprot.2011.431. pCMV Add to Cart
pTALEN_v2 (NN) TALE Nuclease, NN for 0.5 repeat Mammalian Expression A transcription activator-like effector toolbox for genome engineering. Sanjana et al Nat Protoc. 2012 Jan 5;7(1):171-92. doi: 10.1038/nprot.2011.431. pCMV Add to Cart
pTALEN_v2 (HD) TALE Nuclease, HD for 0.5 repeat Mammalian Expression A transcription activator-like effector toolbox for genome engineering. Sanjana et al Nat Protoc. 2012 Jan 5;7(1):171-92. doi: 10.1038/nprot.2011.431. pCMV Add to Cart
pLenti-EF1a-SOX2 TALE-Sox2 (Synthetic) Mammalian Expression, Lentiviral Efficient construction of sequence-specific TAL effectors for modulating mammalian transcription. Zhang et al Nat Biotechnol. 2011 Jan 19. ():. pLenti Add to Cart
pLenti-EF1a-KLF4 TALE-Klf4 (Synthetic) Mammalian Expression, Lentiviral Efficient construction of sequence-specific TAL effectors for modulating mammalian transcription. Zhang et al Nat Biotechnol. 2011 Jan 19. ():. pLenti Add to Cart
pLenti-EF1a-OCT4 TALE-Oct4 (Synthetic) Mammalian Expression, Lentiviral Efficient construction of sequence-specific TAL effectors for modulating mammalian transcription. Zhang et al Nat Biotechnol. 2011 Jan 19. ():. pLenti Add to Cart
TALE5 TALE5 (Synthetic) Mammalian Expression, Lentiviral Efficient construction of sequence-specific TAL effectors for modulating mammalian transcription. Zhang et al Nat Biotechnol. 2011 Jan 19. ():. pLenti
  • Tag / Fusion Protein
    • EGFP (C terminal on insert)
  • Add to Cart
    TALE6 TALE6 (Synthetic) Mammalian Expression, Lentiviral Efficient construction of sequence-specific TAL effectors for modulating mammalian transcription. Zhang et al Nat Biotechnol. 2011 Jan 19. ():. pLenti
  • Tag / Fusion Protein
    • EGFP (C terminal on insert)
  • Add to Cart
    TALE8 TALE8 (Synthetic) Mammalian Expression, Lentiviral Efficient construction of sequence-specific TAL effectors for modulating mammalian transcription. Zhang et al Nat Biotechnol. 2011 Jan 19. ():. pLenti
  • Tag / Fusion Protein
    • EGFP (C terminal on insert)
  • Add to Cart
    TALE10 TALE10 (Synthetic) Mammalian Expression, Lentiviral Efficient construction of sequence-specific TAL effectors for modulating mammalian transcription. Zhang et al Nat Biotechnol. 2011 Jan 19. ():. pLenti
  • Tag / Fusion Protein
    • EGFP (C terminal on insert)
  • Add to Cart
    TALE11 TALE11 (Synthetic) Mammalian Expression, Lentiviral Efficient construction of sequence-specific TAL effectors for modulating mammalian transcription. Zhang et al Nat Biotechnol. 2011 Jan 19. ():. pLenti Add to Cart
    TALE12 TALE12 (Synthetic) Mammalian Expression, Lentiviral Efficient construction of sequence-specific TAL effectors for modulating mammalian transcription. Zhang et al Nat Biotechnol. 2011 Jan 19. ():. pLenti
  • Tag / Fusion Protein
    • EGFP (C terminal on insert)
  • Add to Cart
    hAAVS1 1L TALEN hAAVS1 1L TALEN Mammalian Expression A transcription activator-like effector toolbox for genome engineering. Sanjana et al Nat Protoc. 2012 Jan 5;7(1):171-92. doi: 10.1038/nprot.2011.431. TALEN designed to target the human AAVS1 locus TALEN BB Add to Cart
    hAAVS1 1R TALEN hAAVS1 1R TALEN Mammalian Expression A transcription activator-like effector toolbox for genome engineering. Sanjana et al Nat Protoc. 2012 Jan 5;7(1):171-92. doi: 10.1038/nprot.2011.431. TALEN designed to target the human AAVS1 locus TALEN BB Add to Cart
    Monomer NH_V2 Monomer NH (Synthetic) Comprehensive interrogation of natural TALE DNA-binding modules and transcriptional repressor domains. Cong et al Nat Commun. 2012 Jul 24;3:968. doi: 10.1038/ncomms1962. template for PCR amplification of the monomer used for assembly of the DNA-binding tandem repeat region of the TALE. PJ201 Add to Cart
    Monomer NA_V2 Monomer NA (Synthetic) Comprehensive interrogation of natural TALE DNA-binding modules and transcriptional repressor domains. Cong et al Nat Commun. 2012 Jul 24;3:968. doi: 10.1038/ncomms1962. PJ201 Add to Cart
    pX097-U6-SpTracrRNA-EF1a-hSpRNaseIII-mCherry U6-SpTracrRNA-EF1a-hSpRNaseIII-mCherry Mammalian Expression, Lentiviral, CRISPR Multiplex Genome Engineering Using CRISPR/Cas Systems. Cong et al Science. 2013 Jan 3. This plasmid is used to reconstitute the complete Type II CRISPR system from S. pyogenes and contains the host factor RNaseIII and tracrRNA. pLenti Add to Cart
    pX260-U6-DR-BB-DR-Cbh-NLS-hSpCas9-NLS-H1-shorttracr-PGK-puro humanized S. pyogenes Cas9 Mammalian Expression, CRISPR Multiplex Genome Engineering Using CRISPR/Cas Systems. Cong et al Science. 2013 Jan 3. This plasmid separately encodes a human codon-optimized SpCas9, a tracrRNA and customizable crRNA. pUC ori vector Add to Cart
    pX330-U6-Chimeric_BB-CBh-hSpCas9 humanized S. pyogenes Cas9 Mammalian Expression, CRISPR Multiplex Genome Engineering Using CRISPR/Cas Systems. Cong et al Science. 2013 Jan 3. A human codon-optimized SpCas9 and chimeric guide RNA expression plasmid. pUC ori vector
  • Tag / Fusion Protein
    • 3xFLAG (N terminal on insert)
  • Add to Cart
    pX334-U6-DR-BB-DR-Cbh-NLS-hSpCas9n(D10A)-NLS-H1-shorttracr-PGK-puro humanized S. pyogenes Cas9 (D10A) nickase Mammalian Expression, CRISPR Multiplex Genome Engineering Using CRISPR/Cas Systems. Cong et al Science. 2013 Jan 3. This plasmid separately encodes a human codon-optimized SpCas9 nickase, a tracrRNA and customizable crRNA. pUC ori vector
  • Tag / Fusion Protein
    • HA (N terminal on insert)
  • Add to Cart
    pX335-U6-Chimeric_BB-CBh-hSpCas9n(D10A) humanized S. pyogenes Cas9 (D10A) nickase Mammalian Expression, CRISPR Multiplex Genome Engineering Using CRISPR/Cas Systems. Cong et al Science. 2013 Jan 3. A human codon-optimized SpCas9 nickase and chimeric guide RNA expression plasmid. pUC ori vector
  • Tag / Fusion Protein
    • HA (N terminal on insert)
  • Add to Cart
    pX261-U6-DR-hEmx1-DR-Cbh-NLS-hSpCas9-NLS-H1-shorttracr-PGK-puro humanized S. pyogenes Cas9 Mammalian Expression, CRISPR Multiplex Genome Engineering Using CRISPR/Cas Systems. Cong et al Science. 2013 Jan 3. Dual expression plasmid of human codon-optimized SpCas9 and a gRNA to the human Emx1 locus, can be used to test SpCas9 cleavage in cell lines of choice. pUC ori vector
  • Tag / Fusion Protein
    • 3xFLAG (N terminal on insert)
  • Add to Cart
    pAAV_TALE-TF(VP64)-BB_V3 TALE-TF(VP64)-BB Mammalian Expression ; Adeno-associated Virus (AAV) Comprehensive interrogation of natural TALE DNA-binding modules and transcriptional repressor domains. Cong et al Nat Commun. 2012 Jul 24;3:968. doi: 10.1038/ncomms1962. pAAV Add to Cart
    pAAV_TALEN-FokI(WT)-BB_V3 TALEN-FokI(WT)-BB Mammalian Expression ; Adeno-associated Virus (AAV) Comprehensive interrogation of natural TALE DNA-binding modules and transcriptional repressor domains. Cong et al Nat Commun. 2012 Jul 24;3:968. doi: 10.1038/ncomms1962. pAAV
  • Tag / Fusion Protein
    • 3XFlag (N terminal on backbone)
  • Add to Cart
    SOX2 TALE Repressor (KRAB 1-75) SOX2 TALE Repressor (KRAB 1-75) Mammalian Expression, Lentiviral Comprehensive interrogation of natural TALE DNA-binding modules and transcriptional repressor domains. Cong et al Nat Commun. 2012 Jul 24;3:968. doi: 10.1038/ncomms1962. pLenti
  • Tag / Fusion Protein
    • 2A-GFP (C terminal on insert)
  • Add to Cart
    SOX2 TALE Repressor (KRAB 11-75) SOX2 TALE Repressor (KRAB 11-75) Mammalian Expression, Lentiviral Comprehensive interrogation of natural TALE DNA-binding modules and transcriptional repressor domains. Cong et al Nat Commun. 2012 Jul 24;3:968. doi: 10.1038/ncomms1962. pLenti
  • Tag / Fusion Protein
    • 2A-GFP (C terminal on insert)
  • Add to Cart
    Enhanced Repressor Domain for TAL Effector (SID4X) SID4X Domain Comprehensive interrogation of natural TALE DNA-binding modules and transcriptional repressor domains. Cong et al Nat Commun. 2012 Jul 24;3:968. doi: 10.1038/ncomms1962. pUC57 Add to Cart
    pAAV_hSyn_TALEBB(NN)-NLS-VP64_2A_GFP_WPRE_bGHpA TALE BB(N136_0.5NN_C63)-VP64 (Synthetic) Mammalian Expression, AAV ; TALE Optical control of mammalian endogenous transcription and epigenetic states. Konermann et al Nature. 2013 Jul 23. doi: 10.1038/nature12466. AAV TALE synthesis backbone with NN half repeat; fused to nuclear localization sequence linker and VP64 transcriptional activator effector domain. Synapsin promoter for neuronal expression. pAAV_hSyn_WPRE_bGHpA Add to Cart
    pAAV_hSyn_TALEBB(HD)-NLS-VP64_2A_GFP_WPRE_bGHpA TALE BB(N136_0.5HD_C63)-VP64 (Synthetic) Mammalian Expression, AAV ; TALE Optical control of mammalian endogenous transcription and epigenetic states. Konermann et al Nature. 2013 Jul 23. doi: 10.1038/nature12466. AAV TALE synthesis backbone with HD half repeat; fused to nuclear localization sequence linker and VP64 transcriptional activator effector domain. Synapsin promoter for neuronal expression. pAAV_hSyn_WPRE_bGHpA Add to Cart
    pAAV_hSyn_TALEBB(NG)-NLS-VP64_2A_GFP_WPRE_bGHpA TALE BB(N136_0.5NG_C63)-VP64 (Synthetic) Mammalian Expression, AAV ; TALE Optical control of mammalian endogenous transcription and epigenetic states. Konermann et al Nature. 2013 Jul 23. doi: 10.1038/nature12466. AAV TALE synthesis backbone with NG half repeat; fused to nuclear localization sequence linker and VP64 transcriptional activator effector domain. Synapsin promoter for neuronal expression. pAAV_hSyn_WPRE_bGHpA Add to Cart
    pAAV_hSyn_TALEBB(NI)-NLS-VP64_2A_GFP_WPRE_bGHpA TALE BB(N136_0.5NI_C63)-VP64 (Synthetic) Mammalian Expression, AAV ; TALE Optical control of mammalian endogenous transcription and epigenetic states. Konermann et al Nature. 2013 Jul 23. doi: 10.1038/nature12466. AAV TALE synthesis backbone with NI half repeat; fused to nuclear localization sequence linker and VP64 transcriptional activator effector domain. Synapsin promoter for neuronal expression. pAAV_hSyn_WPRE_bGHpA Add to Cart
    pAAV_hSyn_TALEBB(NN)-NLS-SID4X_2A_phiLOV2.1_WPRE_bGHpA TALE BB(N136_0.5NN_C63)-SID4X (Synthetic) Mammalian Expression, AAV ; TALE Optical control of mammalian endogenous transcription and epigenetic states. Konermann et al Nature. 2013 Jul 23. doi: 10.1038/nature12466. AAV TALE synthesis backbone with NN half repeat; fused to nuclear localization sequence linker and SID4X transcriptional repressor effector domain. Synapsin promoter for neuronal expression. pAAV_hSyn_WPRE_bGHpA Add to Cart
    pAAV_hSyn_TALEBB(HD)-NLS-SID4X_2A_phiLOV2.1_WPRE_bGHpA TALE BB(N136_0.5HD_C63)-SID4X (Synthetic) Mammalian Expression, AAV ; TALE Optical control of mammalian endogenous transcription and epigenetic states. Konermann et al Nature. 2013 Jul 23. doi: 10.1038/nature12466. AAV TALE synthesis backbone with HD half repeat; fused to nuclear localization sequence linker and SID4X transcriptional repressor effector domain. Synapsin promoter for neuronal expression. pAAV_hSyn_WPRE_bGHpA Add to Cart
    pAAV_hSyn_TALEBB(NG)-NLS-SID4X_2A_phiLOV2.1_WPRE_bGHpA TALE BB(N136_0.5NG_C63)-SID4X (Synthetic) Mammalian Expression, AAV ; TALE Optical control of mammalian endogenous transcription and epigenetic states. Konermann et al Nature. 2013 Jul 23. doi: 10.1038/nature12466. AAV TALE synthesis backbone with NG half repeat; fused to nuclear localization sequence linker and SID4X transcriptional repressor effector domain. Synapsin promoter for neuronal expression. pAAV_hSyn_WPRE_bGHpA Add to Cart
    pAAV_hSyn_TALEBB(NI)-NLS-SID4X_2A_phiLOV2.1_WPRE_bGHpA TALE BB(N136_0.5NI_C63)-SID4X (Synthetic) Mammalian Expression, AAV ; TALE Optical control of mammalian endogenous transcription and epigenetic states. Konermann et al Nature. 2013 Jul 23. doi: 10.1038/nature12466. AAV TALE synthesis backbone with NI half repeat; fused to nuclear localization sequence linker and SID4X transcriptional repressor effector domain. Synapsin promoter for neuronal expression. pAAV_hSyn_WPRE_bGHpA Add to Cart
    LITE1.0_pAAV_hSyn_TALE(Grm2)-NLS-CIB1_2A_GFP_WPRE_bGHpA TALE (N136,Grm2,C63)-cib1 (Synthetic) Mammalian Expression, AAV ; TALE Optical control of mammalian endogenous transcription and epigenetic states. Konermann et al Nature. 2013 Jul 23. doi: 10.1038/nature12466. epiLITE / LITE1.0 TALE-CIB1. CIB1 binds to blue-light-activated CRY2PHR. Particular TALE targeted to Grm2 promoter. Synapsin promoter for neuronal expression. pAAV_hSyn_WPRE_bGHpA Add to Cart
    LITE1.0_pAAV_hSyn_CRY2PHR-NLS-SID4X_2A_phiLOV2.1_WPRE_bGHpA CRY2PHR-NLS-SID4X (Synthetic) Mammalian Expression, AAV Optical control of mammalian endogenous transcription and epigenetic states. Konermann et al Nature. 2013 Jul 23. doi: 10.1038/nature12466. epiLITE / LITE1.0 CRY2PHR fused to SID4X transcriptional repressor effector domain. Binds to CIB1 upon blue light stimulation. Synapsin promoter for neuronal expression. pAAV_hSyn_WPRE_bGHpA Add to Cart
    LITE2.0 pAAV_hSyn_NLS(alpha-imp)-CRY2PHR-NLS-VP64_2A_GFP_WPRE_bGHpA NLS(alpha-imp)-CRY2PHR-NLS-VP64 (Synthetic) Mammalian Expression, AAV Optical control of mammalian endogenous transcription and epigenetic states. Konermann et al Nature. 2013 Jul 23. doi: 10.1038/nature12466. LITE2.0 CRY2PHR-VP64. Changes from LITE1.0: N-terminal alpha-importin nuclear localization sequence. Synapsin promoter for neuronal expression. pAAV_hSyn_WPRE_bGHpA Add to Cart
    LITE2.0_pAAV_hSyn_TALE(Grm2)-GS-CIB1(NLS*,∆318-334)_WPRE_bGHpA TALE(Grm2)-GS-CIB1(NLS*,∆318-334) (Synthetic) Mammalian Expression, AAV ; TALE Optical control of mammalian endogenous transcription and epigenetic states. Konermann et al Nature. 2013 Jul 23. doi: 10.1038/nature12466. LITE2.0 TALE-CIB1. Changes from LITE1.0: glycine-serine linker, mutated endogenous NLS, aa318-334 deletion. This particular TALE targeted to Grm2. Synapsin promoter for neuronal expression. pAAV_hSyn_WPRE_bGHpA Add to Cart
    LITE2.0 EF1a_NLS(alpha-imp)-CRY2PHR-NLS-VP64_2A_GFP_WPRE_hGHpA NLS(alpha-imp)-CRY2PHR-NLS-VP64 (Synthetic) Mammalian Expression Optical control of mammalian endogenous transcription and epigenetic states. Konermann et al Nature. 2013 Jul 23. doi: 10.1038/nature12466. LITE2.0 CRY2PHR-VP64. Changes from LITE1.0: N-terminal alpha-importin nuclear localization sequence. EF1-alpha promoter for ubiquitous mammalian expression. pAAV_EF1a_WPRE_hGHpA Add to Cart
    LITE2.0 EF1a_TALE(Neurog2)-GS-CIB1(NLS*,∆318-334)_WPRE_hGHpA TALE (Neurog2)-GS-CIB1(NLS*,∆318-334) (Synthetic) Mammalian Expression Optical control of mammalian endogenous transcription and epigenetic states. Konermann et al Nature. 2013 Jul 23. doi: 10.1038/nature12466. LITE2.0 TALE-CIB1. Changes from LITE1.0: glycine-serine linker, mutated endogenous NLS, aa318-334 deletion. This particular TALE targeted to Neurog2. EF1-alpha promoter for ubiquitous mammalian exp. pAAV_EF1a_WPRE_hGHpA Add to Cart
    pSpCas9 (PX165) hSpCas9 (Synthetic) Mammalian Expression, CRISPR Genome engineering using the CRISPR-Cas9 system. Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi: 10.1038/nprot.2013.143. Epub 2013 Oct 24. Human codon optimized Cas9 nuclease from Streptococcus pyogenes (Cbh-3X-FLAG-NLS-SpCas9-NLS) PX165 Add to Cart
    pSpCas9(BB)-2A-GFP (PX458) hSpCas9 (Synthetic) Mammalian Expression, CRISPR Genome engineering using the CRISPR-Cas9 system. Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi: 10.1038/nprot.2013.143. Epub 2013 Oct 24. Cas9 from S. pyogenes with 2A-EGFP, and cloning backbone for sgRNA PX458 Add to Cart
    pSpCas9(BB)-2A-Puro (PX459) hSpCas9-2A-Puro (Synthetic) Mammalian Expression, CRISPR Genome engineering using the CRISPR-Cas9 system. Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi: 10.1038/nprot.2013.143. Epub 2013 Oct 24. Cas9 from S. pyogenes with 2A-Puro, and cloning backbone for sgRNA PX459 Add to Cart
    pSpCas9n(BB)-2A-GFP (PX461) hSpCas9n (Synthetic) Mammalian Expression, CRISPR Genome engineering using the CRISPR-Cas9 system. Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi: 10.1038/nprot.2013.143. Epub 2013 Oct 24. Cas9n (D10A nickase mutant) from S. pyogenes with 2A-EGFP, and cloning backbone for sgRNA PX461 Add to Cart
    pSpCas9n(BB)-2A-Puro (PX462) hSpCas9n-2A-Puro (Synthetic) Mammalian Expression, CRISPR Genome engineering using the CRISPR-Cas9 system. Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi: 10.1038/nprot.2013.143. Epub 2013 Oct 24. Cas9n (D10A nickase mutant) from S. pyogenes with 2A-Puro, and cloning backbone for sgRNA PX462 Add to Cart
    LITE1.0_pAAV_hSyn_CRY2PHR-NLS-VP64_2A_GFP_WPRE_bGHpA CRY2PHR-NLS-VP64 (Synthetic) Mammalian Expression, AAV ; TALE Optical control of mammalian endogenous transcription and epigenetic states. Konermann et al Nature. 2013 Jul 23. doi: 10.1038/nature12466. LITE1.0 CRY2PHR fused to VP64 transcriptional activator domain. Binds to CIB1 upon blue light stimulation. Synapsin promoter for neuronal expression. See LITE2.0 for optimized LITE activators. pAAV_hSyn_WPRE_bGHpA Add to Cart
    pSpCas9n(BB) (PX460) hSpCas9n (Synthetic) Mammalian Expression, CRISPR Genome engineering using the CRISPR-Cas9 system. Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi: 10.1038/nprot.2013.143. Epub 2013 Oct 24. Cas9n (D10A nickase mutant) from S. pyogenes PX460 Add to Cart
    lentiCRISPR - EGFP sgRNA 1 Cas9 (Synthetic), Puromycin resistance (Other), EGFP sgRNA 1 (Synthetic) Mammalian Expression, Lentiviral, CRISPR Genome-scale CRISPR-Cas9 knockout screening in human cells. Shalem et al Science. 2014 Jan 3;343(6166):84-7. doi: 10.1126/science.1247005. Epub 2013 Dec 12. Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an EGFP targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone. lentiCRISPR (pXPR_001), Addgene plasmid #49535 Add to Cart
    lentiCRISPR - EGFP sgRNA 2 Cas9 (Synthetic), Puromycin resistance (Other), EGFP sgRNA 2 (Synthetic) Mammalian Expression, Lentiviral, CRISPR Genome-scale CRISPR-Cas9 knockout screening in human cells. Shalem et al Science. 2014 Jan 3;343(6166):84-7. doi: 10.1126/science.1247005. Epub 2013 Dec 12. Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an EGFP targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone. lentiCRISPR (pXPR_001), Addgene plasmid #49535 Add to Cart
    lentiCRISPR - EGFP sgRNA 3 Cas9 (Synthetic), Puromycin resistance (Other), EGFP sgRNA 3 (Synthetic) Mammalian Expression, Lentiviral, CRISPR Genome-scale CRISPR-Cas9 knockout screening in human cells. Shalem et al Science. 2014 Jan 3;343(6166):84-7. doi: 10.1126/science.1247005. Epub 2013 Dec 12. Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an EGFP targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone. lentiCRISPR (pXPR_001), Addgene plasmid #49535 Add to Cart
    lentiCRISPR - EGFP sgRNA 4 Cas9 (Synthetic), Puromycin resistance (Other), EGFP sgRNA 4 (Synthetic) Mammalian Expression, Lentiviral, CRISPR Genome-scale CRISPR-Cas9 knockout screening in human cells. Shalem et al Science. 2014 Jan 3;343(6166):84-7. doi: 10.1126/science.1247005. Epub 2013 Dec 12. Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an EGFP targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone. lentiCRISPR (pXPR_001), Addgene plasmid #49535 Add to Cart
    lentiCRISPR - EGFP sgRNA 5 Cas9 (Synthetic), Puromycin resistance (Other), EGFP sgRNA 5 (Synthetic) Lentiviral, CRISPR Genome-scale CRISPR-Cas9 knockout screening in human cells. Shalem et al Science. 2014 Jan 3;343(6166):84-7. doi: 10.1126/science.1247005. Epub 2013 Dec 12. Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an EGFP targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone. lentiCRISPR (pXPR_001), Addgene plasmid #49535 Add to Cart
    lentiCRISPR - EGFP sgRNA 6 Cas9 (Synthetic), Puromycin resistance (Other), EGFP sgRNA 6 (Synthetic) Mammalian Expression, Lentiviral, CRISPR Genome-scale CRISPR-Cas9 knockout screening in human cells. Shalem et al Science. 2014 Jan 3;343(6166):84-7. doi: 10.1126/science.1247005. Epub 2013 Dec 12. Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an EGFP targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone. lentiCRISPR (pXPR_001), Addgene plasmid #49535 Add to Cart
    lentiCRISPR v2 Cas9 (Synthetic), Puromycin resistance Mammalian Expression, Lentiviral, CRISPR Improved vectors and genome-wide libraries for CRISPR screening. Sanjana et al Nat Methods. 2014 Aug;11(8):783-4. doi: 10.1038/nmeth.3047. Replaces original lentiCRISPRv1 (Addgene Plasmid 49535) and produces ~10-fold higher titer virus. Custom Add to Cart
    lentiCas9-Blast Cas9 (Synthetic), Blasticidin resistance Mammalian Expression, Lentiviral, CRISPR Improved vectors and genome-wide libraries for CRISPR screening. Sanjana et al Nat Methods. 2014 Aug;11(8):783-4. doi: 10.1038/nmeth.3047. Expresses human codon-optimized S. pyogenes Cas9 protein and blasticidin resistance from EFS promoter. Lentiviral backbone. pFUGW Add to Cart
    lentiGuide-Puro S. pyogenes sgRNA cassette (Synthetic), Puromycin resistance Mammalian Expression, Lentiviral, CRISPR Improved vectors and genome-wide libraries for CRISPR screening. Sanjana et al Nat Methods. 2014 Aug;11(8):783-4. doi: 10.1038/nmeth.3047. Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and puromycin resistance from EF-1a promoter. Lentiviral backbone. Custom Add to Cart
    PX543 SpCas9(del175-307) hSpCas9 (Synthetic) Mammalian Expression, CRISPR Crystal structure of Cas9 in complex with guide RNA and target DNA. Nishimasu et al Cell. 2014 Feb 27;156(5):935-49. doi: 10.1016/j.cell.2014.02.001. Epub 2014 Feb 13. Truncated form of SpCas9 without REC2 domain PX165 Add to Cart
    PX544 SpCas9(del1098-1368) hSpCas9 (Synthetic) Mammalian Expression, CRISPR Crystal structure of Cas9 in complex with guide RNA and target DNA. Nishimasu et al Cell. 2014 Feb 27;156(5):935-49. doi: 10.1016/j.cell.2014.02.001. Epub 2014 Feb 13. Truncated form of SpCas9 without C' domain PX165 Add to Cart
    AAV:ITR-U6-sgRNA(Kras)-U6-sgRNA(p53)-U6-sgRNA(Lkb1)-pEFS-Rluc-2A-Cre-shortPA-KrasG12D_HDRdonor-ITR (AAV-KPL) sgRNA (Synthetic), Renilla luciferase, Cre recombinase, KrasG12D HDR donor Mammalian Expression, Mouse Targeting, AAV, Cre/Lox, CRISPR CRISPR-Cas9 Knockin Mice for Genome Editing and Cancer Modeling. Platt et al Cell. 2014 Sep 24. pii: S0092-8674(14)01163-5. doi: 10.1016/j.cell.2014.09.014. Expresses Cre recombinase and Renilla luciferase from the EFS promoter and three U6-driven sgRNAs targeting Kras, p53, and Lkb1. Contains KrasG12D HDR donor. AAV backbone. AAV Add to Cart
    AAV:ITR-U6-sgRNA(LacZ)-pEFS-Rluc-2A-Cre-WPRE-hGHpA-ITR sgRNA, Renilla luciferase, Cre recombinase Mammalian Expression, Mouse Targeting, AAV, Cre/Lox, CRISPR, Luciferase CRISPR-Cas9 Knockin Mice for Genome Editing and Cancer Modeling. Platt et al Cell. 2014 Sep 24. pii: S0092-8674(14)01163-5. doi: 10.1016/j.cell.2014.09.014. Expresses Cre recombinase and Renilla luciferase from the EFS promoter and one U6-driven sgRNA targeting LacZ. AAV backbone. AAV Add to Cart
    AAV:ITR-U6-sgRNA(backbone)-pEFS-Rluc-2A-Cre-WPRE-hGHpA-ITR sgRNA, Renilla luciferase, Cre recombinase Mammalian Expression, Mouse Targeting, AAV, Cre/Lox, CRISPR, Luciferase CRISPR-Cas9 Knockin Mice for Genome Editing and Cancer Modeling. Platt et al Cell. 2014 Sep 24. pii: S0092-8674(14)01163-5. doi: 10.1016/j.cell.2014.09.014. Expresses Cre recombinase and Renilla luciferase from the EFS promoter and one U6-driven sgRNA. AAV backbone with SapI spacer for sgRNA cloning. AAV Add to Cart
    AAV:ITR-U6-sgRNA(NeuN)-pCBh-Cre-WPRE-hGHpA-ITR sgRNA, Cre recombinase Mammalian Expression, Mouse Targeting, AAV, Cre/Lox, CRISPR CRISPR-Cas9 Knockin Mice for Genome Editing and Cancer Modeling. Platt et al Cell. 2014 Sep 24. pii: S0092-8674(14)01163-5. doi: 10.1016/j.cell.2014.09.014. Expresses Cre recombinase from the Cbh promoter and one U6-driven sgRNA targeting the mouse gene NeuN. AAV backbone. AAV Add to Cart
    AAV:ITR-U6-sgRNA(LacZ)-pCBh-Cre-WPRE-hGHpA-ITR sgRNA, Cre recombinase Mammalian Expression, Mouse Targeting, AAV, Cre/Lox, CRISPR CRISPR-Cas9 Knockin Mice for Genome Editing and Cancer Modeling. Platt et al Cell. 2014 Sep 24. pii: S0092-8674(14)01163-5. doi: 10.1016/j.cell.2014.09.014. Expresses Cre recombinase from the Cbh promoter and one U6-driven sgRNA control targeting LacZ. AAV backbone. AAV Add to Cart
    AAV:ITR-U6-sgRNA(backbone)-pCBh-Cre-WPRE-hGHpA-ITR sgRNA, Cre recombinase Mammalian Expression, Mouse Targeting, AAV, Cre/Lox, CRISPR CRISPR-Cas9 Knockin Mice for Genome Editing and Cancer Modeling. Platt et al Cell. 2014 Sep 24. pii: S0092-8674(14)01163-5. doi: 10.1016/j.cell.2014.09.014. Expresses Cre recombinase from the Cbh promoter and one U6-driven sgRNA. AAV backbone with SapI spacer for sgRNA cloning. AAV Add to Cart
    AAV:ITR-U6-sgRNA(NeuN)-hSyn-Cre-2A-EGFP-KASH-WPRE-shortPA-ITR sgRNA, Cre recombinase, EGFP Mammalian Expression, Mouse Targeting, AAV, Cre/Lox, CRISPR CRISPR-Cas9 Knockin Mice for Genome Editing and Cancer Modeling. Platt et al Cell. 2014 Sep 24. pii: S0092-8674(14)01163-5. doi: 10.1016/j.cell.2014.09.014. Expresses Cre recombinase and KASH-tagged EGFP from the hSyn promoter and one U6-driven sgRNA. AAV backbone with SapI spacer for sgRNA cloning. AAV Add to Cart
    AAV:ITR-U6-sgRNA(backbone)-hSyn-Cre-2A-EGFP-KASH-WPRE-shortPA-ITR sgRNA, Cre recombinase, EGFP Mammalian Expression, Mouse Targeting, AAV, Cre/Lox, CRISPR CRISPR-Cas9 Knockin Mice for Genome Editing and Cancer Modeling. Platt et al Cell. 2014 Sep 24. pii: S0092-8674(14)01163-5. doi: 10.1016/j.cell.2014.09.014. Expresses Cre recombinase and KASH-tagged EGFP from the hSyn promoter and one U6-driven sgRNA. AAV backbone with SapI spacer for sgRNA cloning. AAV Add to Cart
    PX551 SpCas9 (Synthetic) Mammalian Expression, AAV, CRISPR In vivo interrogation of gene function in the mammalian brain using CRISPR-Cas9. Swiech et al Nat Biotechnol. 2014 Oct 19. doi: 10.1038/nbt.3055. pAAV-pMecp2-SpCas9-spA (AAV-SpCas9). AAV plasmid expressing Cas9 in neurons under control of truncated mecp2 promoter. pAAV Add to Cart
    PX552 U6_(SpaI)_sgRNA (Synthetic), Syn_EGFP-KASH (Synthetic) Mammalian Expression, Mouse Targeting, AAV, CRISPR In vivo interrogation of gene function in the mammalian brain using CRISPR-Cas9. Swiech et al Nat Biotechnol. 2014 Oct 19. doi: 10.1038/nbt.3055. pAAV-U6sgRNA(SapI)_hSyn-GFP-KASH-bGH (SpGuide acceptor). AAV plasmid for sgRNA cloning. GFP-KASH fusion facilitates FACS sorting of cells and nuclei. pAAV Add to Cart
    dCAS9-VP64_GFP dCAS9(D10A,H840A)-VP64_2A_GFP (Synthetic) Mammalian Expression, Lentiviral, CRISPR Genome-scale transcriptional activation by an engineered CRISPR-Cas9 complex. Konermann et al Nature. 2014 Dec 10. doi: 10.1038/nature14136. Expresses dCAS9-VP64 activator with 2A GFP lenti(AMP) Add to Cart
    MS2-P65-HSF1_GFP MS2(N55K)-P65-HSF1_2A_GFP (Homo sapiens) Mammalian Expression, CRISPR Genome-scale transcriptional activation by an engineered CRISPR-Cas9 complex. Konermann et al Nature. 2014 Dec 10. doi: 10.1038/nature14136. Expresses the MS2-P65-HSF1 activator helper complex with a 2A GFP lenti(AMP) Add to Cart
    sgRNA(MS2) cloning backbone Mammalian Expression, CRISPR Genome-scale transcriptional activation by an engineered CRISPR-Cas9 complex. Konermann et al Nature. 2014 Dec 10. doi: 10.1038/nature14136. sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2. Contains BbsI sites for insertion of spacer sequences. pUC19 Add to Cart
    lenti dCAS-VP64_Blast dCAS9(D10A, N863A)-VP64_2A_Blast (Synthetic) Mammalian Expression, Lentiviral, CRISPR Genome-scale transcriptional activation by an engineered CRISPR-Cas9 complex. Konermann et al Nature. 2014 Dec 10. doi: 10.1038/nature14136. lenti vector encoding dCAS9-VP64 with 2A Blast resistance marker (EF1a-NLS-dCas9(N863)-VP64-2A-Blast-WPRE) plenti Add to Cart
    lenti MS2-P65-HSF1_Hygro MS2-P65-HSF1_2A_Hygro (Homo sapiens) Mammalian Expression, Lentiviral, CRISPR Genome-scale transcriptional activation by an engineered CRISPR-Cas9 complex. Konermann et al Nature. 2014 Dec 10. doi: 10.1038/nature14136. lenti vector encoding the MS2-P65-HSF1 activator helper complex with a 2A Hygro resistance marker (EF1a-MS2-p65-HSF1-2A-Hygro-WPRE) plenti Add to Cart
    lenti sgRNA(MS2)_zeo backbone Mammalian Expression, Lentiviral, CRISPR Genome-scale transcriptional activation by an engineered CRISPR-Cas9 complex. Konermann et al Nature. 2014 Dec 10. doi: 10.1038/nature14136. lenti sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2 and EF1a-zeo resistance marker. Contains BsmBI sites for insertion of spacer sequences. plenti Add to Cart