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Feng Zhang Lab Plasmids


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Title Authors Publication
In vivo genome editing using Staphylococcus aureus Cas9. Ran FA, Cong L, Yan WX, Scott DA, Gootenberg JS, Kriz AJ, Zetsche B, Shalem O, Wu X, Makarova KS, Koonin EV, Sharp PA, Zhang F Nature. 2015 Apr 1. doi: 10.1038/nature14299.
Genome-wide CRISPR screen in a mouse model of tumor growth and metastasis. Chen S, Sanjana NE, Zheng K, Shalem O, Lee K, Shi X, Scott DA, Song J, Pan JQ, Weissleder R, Lee H, Zhang F, Sharp PA Cell. 2015 Mar 12;160(6):1246-60. doi: 10.1016/j.cell.2015.02.038. Epub 2015 Mar 5.
A split-Cas9 architecture for inducible genome editing and transcription modulation. Zetsche B, Volz SE, Zhang F Nat Biotechnol. 2015 Feb 2. doi: 10.1038/nbt.3149.
Genome-scale transcriptional activation by an engineered CRISPR-Cas9 complex. Konermann S, Brigham MD, Trevino AE, Joung J, Abudayyeh OO, Barcena C, Hsu PD, Habib N, Gootenberg JS, Nishimasu H, Nureki O, Zhang F Nature. 2014 Dec 10. doi: 10.1038/nature14136.
In vivo interrogation of gene function in the mammalian brain using CRISPR-Cas9. Swiech L, Heidenreich M, Banerjee A, Habib N, Li Y, Trombetta J, Sur M, Zhang F Nat Biotechnol. 2014 Oct 19. doi: 10.1038/nbt.3055.
CRISPR-Cas9 Knockin Mice for Genome Editing and Cancer Modeling. Platt RJ, Chen S, Zhou Y, Yim MJ, Swiech L, Kempton HR, Dahlman JE, Parnas O, Eisenhaure TM, Jovanovic M, Graham DB, Jhunjhunwala S, Heidenreich M, Xavier RJ, Langer R, Anderson DG, Hacohen N, Regev A, Feng G, Sharp PA, Zhang F Cell. 2014 Sep 24. pii: S0092-8674(14)01163-5. doi: 10.1016/j.cell.2014.09.014.
Improved vectors and genome-wide libraries for CRISPR screening. Sanjana NE, Shalem O, Zhang F Nat Methods. 2014 Aug;11(8):783-4. doi: 10.1038/nmeth.3047.
Crystal structure of Cas9 in complex with guide RNA and target DNA. Nishimasu H, Ran FA, Hsu PD, Konermann S, Shehata SI, Dohmae N, Ishitani R, Zhang F, Nureki O Cell. 2014 Feb 27;156(5):935-49. doi: 10.1016/j.cell.2014.02.001. Epub 2014 Feb 13.
Genome-scale CRISPR-Cas9 knockout screening in human cells. Shalem O, Sanjana NE, Hartenian E, Shi X, Scott DA, Mikkelsen TS, Heckl D, Ebert BL, Root DE, Doench JG, Zhang F Science. 2014 Jan 3;343(6166):84-7. doi: 10.1126/science.1247005. Epub 2013 Dec 12.
Genome engineering using the CRISPR-Cas9 system. Ran FA, Hsu PD, Wright J, Agarwala V, Scott DA, Zhang F Nat Protoc. 2013 Nov;8(11):2281-308. doi: 10.1038/nprot.2013.143. Epub 2013 Oct 24.
Optical control of mammalian endogenous transcription and epigenetic states. Konermann S, Brigham MD, Trevino A, Hsu PD, Heidenreich M, Le Cong, Platt RJ, Scott DA, Church GM, Zhang F Nature. 2013 Jul 23. doi: 10.1038/nature12466.
Multiplex Genome Engineering Using CRISPR/Cas Systems. Cong L, Ran FA, Cox D, Lin S, Barretto R, Habib N, Hsu PD, Wu X, Jiang W, Marraffini LA, Zhang F Science. 2013 Jan 3.
Comprehensive interrogation of natural TALE DNA-binding modules and transcriptional repressor domains. Cong L, Zhou R, Kuo YC, Cunniff M, Zhang F Nat Commun. 2012 Jul 24;3:968. doi: 10.1038/ncomms1962.
A transcription activator-like effector toolbox for genome engineering. Sanjana NE, Cong L, Zhou Y, Cunniff MM, Feng G, Zhang F Nat Protoc. 2012 Jan 5;7(1):171-92. doi: 10.1038/nprot.2011.431.
Efficient construction of sequence-specific TAL effectors for modulating mammalian transcription. Zhang F, Cong L, Lodato S, Kosuri S, Church GM, Arlotta P Nat Biotechnol. 2011 Jan 19. ():.
PX405 Neisseria meningiditis Cas9 (unpublished)
PX377 Corynebacter diphtheriae (unpublished)
Zhang SAM library (unpublished)
TALE Nuclease (TALEN) (unpublished)
ID Plasmid Gene/Insert Vector Type Publication Hidden Extra Search Info  
27961 pLenti-EF1a-Backbone(NN) TAL-Backbone(NN) (Synthetic) Mammalian Expression, Lentiviral Efficient construction of sequence-specific TAL effectors for modulating mammalian transcription. Nat Biotechnol. 2011 Jan 19. ():. pLenti Add to Cart
27962 pLenti-EF1a-Backbone(NI) TAL-Backbone(NI) (Synthetic) Mammalian Expression, Lentiviral Efficient construction of sequence-specific TAL effectors for modulating mammalian transcription. Nat Biotechnol. 2011 Jan 19. ():. pLenti Add to Cart
27963 pLenti-EF1a-Backbone(NG) TAL-Backbone(NG) (Synthetic) Mammalian Expression, Lentiviral Efficient construction of sequence-specific TAL effectors for modulating mammalian transcription. Nat Biotechnol. 2011 Jan 19. ():. pLenti Add to Cart
27964 pLenti-EF1a-Backbone(HD) TAL-Backbone(HD) (Synthetic) Mammalian Expression, Lentiviral Efficient construction of sequence-specific TAL effectors for modulating mammalian transcription. Nat Biotechnol. 2011 Jan 19. ():. pLenti Add to Cart
27965 monomer-NN TAL-monomer-NN Bacterial Expression Efficient construction of sequence-specific TAL effectors for modulating mammalian transcription. Nat Biotechnol. 2011 Jan 19. ():. pJ201 Add to Cart
27966 monomer-NI TAL-monomer-NI Bacterial Expression Efficient construction of sequence-specific TAL effectors for modulating mammalian transcription. Nat Biotechnol. 2011 Jan 19. ():. pJ201 Add to Cart
27967 monomer-NG TAL-monomer-NG Bacterial Expression Efficient construction of sequence-specific TAL effectors for modulating mammalian transcription. Nat Biotechnol. 2011 Jan 19. ():. pJ201 Add to Cart
27968 monomer-HD TAL-monomer-HD Bacterial Expression Efficient construction of sequence-specific TAL effectors for modulating mammalian transcription. Nat Biotechnol. 2011 Jan 19. ():. pJ201 Add to Cart
27969 pLenti-EF1a-TALE1 TALE1 (Synthetic) Mammalian Expression, Lentiviral Efficient construction of sequence-specific TAL effectors for modulating mammalian transcription. Nat Biotechnol. 2011 Jan 19. ():. pLenti Add to Cart
27970 pAAV-minCMV-mCherry mCherry (Synthetic) Mammalian Expression, AAV Efficient construction of sequence-specific TAL effectors for modulating mammalian transcription. Nat Biotechnol. 2011 Jan 19. ():. pAAV Add to Cart
31178 Monomer NK Monomer-NK Bacterial Expression TALE Nuclease (TALEN) (unpublished) pJ201 Add to Cart
31179 TALEN Backbone TALE Nuclease Backbone Mammalian Expression TALE Nuclease (TALEN) (unpublished) pCMV Add to Cart
32180 pNI_v2 TALE Monomer NI Bacterial Expression A transcription activator-like effector toolbox for genome engineering. Nat Protoc. 2012 Jan 5;7(1):171-92. doi: 10.1038/nprot.2011.431. pJ201 Add to Cart
32181 pNG_v2 TALE Monomer NG Bacterial Expression A transcription activator-like effector toolbox for genome engineering. Nat Protoc. 2012 Jan 5;7(1):171-92. doi: 10.1038/nprot.2011.431. pJ201 Add to Cart
32182 pNN_v2 TALE Monomer NN Bacterial Expression A transcription activator-like effector toolbox for genome engineering. Nat Protoc. 2012 Jan 5;7(1):171-92. doi: 10.1038/nprot.2011.431. pJ201 Add to Cart
32183 pHD_v2 TALE Monomer HD Bacterial Expression A transcription activator-like effector toolbox for genome engineering. Nat Protoc. 2012 Jan 5;7(1):171-92. doi: 10.1038/nprot.2011.431. pJ201 Add to Cart
32185 pTALETF_v2 (NN) TALE Transcriptional Activator Backbone, NN for 0.5 repeat Mammalian Expression A transcription activator-like effector toolbox for genome engineering. Nat Protoc. 2012 Jan 5;7(1):171-92. doi: 10.1038/nprot.2011.431. pCMV Add to Cart
32186 pTALETF_v2 (NG) TALE Transcriptional Activator Backbone, NG for 0.5 repeat Mammalian Expression A transcription activator-like effector toolbox for genome engineering. Nat Protoc. 2012 Jan 5;7(1):171-92. doi: 10.1038/nprot.2011.431. pCMV Add to Cart
32187 pTALETF_v2 (NI) TALE Transcriptional Activator Backbone, NI for 0.5 repeat Mammalian Expression A transcription activator-like effector toolbox for genome engineering. Nat Protoc. 2012 Jan 5;7(1):171-92. doi: 10.1038/nprot.2011.431. pCMV Add to Cart
32188 pTALETF_v2 (HD) TALE Transcriptional Activator Backbone, HD for 0.5 repeat Mammalian Expression A transcription activator-like effector toolbox for genome engineering. Nat Protoc. 2012 Jan 5;7(1):171-92. doi: 10.1038/nprot.2011.431. pCMV Add to Cart
32189 pTALEN_v2 (NI) TALE Nuclease, NI for 0.5 repeat Mammalian Expression A transcription activator-like effector toolbox for genome engineering. Nat Protoc. 2012 Jan 5;7(1):171-92. doi: 10.1038/nprot.2011.431. pCMV Add to Cart
32190 pTALEN_v2 (NG) TALE Nuclease, NG for 0.5 repeat Mammalian Expression A transcription activator-like effector toolbox for genome engineering. Nat Protoc. 2012 Jan 5;7(1):171-92. doi: 10.1038/nprot.2011.431. pCMV Add to Cart
32191 pTALEN_v2 (NN) TALE Nuclease, NN for 0.5 repeat Mammalian Expression A transcription activator-like effector toolbox for genome engineering. Nat Protoc. 2012 Jan 5;7(1):171-92. doi: 10.1038/nprot.2011.431. pCMV Add to Cart
32192 pTALEN_v2 (HD) TALE Nuclease, HD for 0.5 repeat Mammalian Expression A transcription activator-like effector toolbox for genome engineering. Nat Protoc. 2012 Jan 5;7(1):171-92. doi: 10.1038/nprot.2011.431. pCMV Add to Cart
35388 pLenti-EF1a-SOX2 TALE-Sox2 (Synthetic) Mammalian Expression, Lentiviral Efficient construction of sequence-specific TAL effectors for modulating mammalian transcription. Nat Biotechnol. 2011 Jan 19. ():. pLenti Add to Cart
35389 pLenti-EF1a-KLF4 TALE-Klf4 (Synthetic) Mammalian Expression, Lentiviral Efficient construction of sequence-specific TAL effectors for modulating mammalian transcription. Nat Biotechnol. 2011 Jan 19. ():. pLenti Add to Cart
35391 pLenti-EF1a-OCT4 TALE-Oct4 (Synthetic) Mammalian Expression, Lentiviral Efficient construction of sequence-specific TAL effectors for modulating mammalian transcription. Nat Biotechnol. 2011 Jan 19. ():. pLenti Add to Cart
35420 TALE5 TALE5 (Synthetic) Mammalian Expression, Lentiviral Efficient construction of sequence-specific TAL effectors for modulating mammalian transcription. Nat Biotechnol. 2011 Jan 19. ():. pLenti
  • Tag / Fusion Protein
    • EGFP (C terminal on insert)
  • Add to Cart
    35421 TALE6 TALE6 (Synthetic) Mammalian Expression, Lentiviral Efficient construction of sequence-specific TAL effectors for modulating mammalian transcription. Nat Biotechnol. 2011 Jan 19. ():. pLenti
  • Tag / Fusion Protein
    • EGFP (C terminal on insert)
  • Add to Cart
    35423 TALE8 TALE8 (Synthetic) Mammalian Expression, Lentiviral Efficient construction of sequence-specific TAL effectors for modulating mammalian transcription. Nat Biotechnol. 2011 Jan 19. ():. pLenti
  • Tag / Fusion Protein
    • EGFP (C terminal on insert)
  • Add to Cart
    35425 TALE10 TALE10 (Synthetic) Mammalian Expression, Lentiviral Efficient construction of sequence-specific TAL effectors for modulating mammalian transcription. Nat Biotechnol. 2011 Jan 19. ():. pLenti
  • Tag / Fusion Protein
    • EGFP (C terminal on insert)
  • Add to Cart
    35426 TALE11 TALE11 (Synthetic) Mammalian Expression, Lentiviral Efficient construction of sequence-specific TAL effectors for modulating mammalian transcription. Nat Biotechnol. 2011 Jan 19. ():. pLenti Add to Cart
    35427 TALE12 TALE12 (Synthetic) Mammalian Expression, Lentiviral Efficient construction of sequence-specific TAL effectors for modulating mammalian transcription. Nat Biotechnol. 2011 Jan 19. ():. pLenti
  • Tag / Fusion Protein
    • EGFP (C terminal on insert)
  • Add to Cart
    35431 hAAVS1 1L TALEN hAAVS1 1L TALEN Mammalian Expression A transcription activator-like effector toolbox for genome engineering. Nat Protoc. 2012 Jan 5;7(1):171-92. doi: 10.1038/nprot.2011.431. TALEN designed to target the human AAVS1 locus TALEN BB Add to Cart
    35432 hAAVS1 1R TALEN hAAVS1 1R TALEN Mammalian Expression A transcription activator-like effector toolbox for genome engineering. Nat Protoc. 2012 Jan 5;7(1):171-92. doi: 10.1038/nprot.2011.431. TALEN designed to target the human AAVS1 locus TALEN BB Add to Cart
    37527 Monomer NH_V2 Monomer NH (Synthetic) Comprehensive interrogation of natural TALE DNA-binding modules and transcriptional repressor domains. Nat Commun. 2012 Jul 24;3:968. doi: 10.1038/ncomms1962. template for PCR amplification of the monomer used for assembly of the DNA-binding tandem repeat region of the TALE. PJ201 Add to Cart
    37529 Monomer NA_V2 Monomer NA (Synthetic) Comprehensive interrogation of natural TALE DNA-binding modules and transcriptional repressor domains. Nat Commun. 2012 Jul 24;3:968. doi: 10.1038/ncomms1962. PJ201 Add to Cart
    41863 pX097-U6-SpTracrRNA-EF1a-hSpRNaseIII-mCherry U6-SpTracrRNA-EF1a-hSpRNaseIII-mCherry Mammalian Expression, Lentiviral, CRISPR Multiplex Genome Engineering Using CRISPR/Cas Systems. Science. 2013 Jan 3. This plasmid is used to reconstitute the complete Type II CRISPR system from S. pyogenes and contains the host factor RNaseIII and tracrRNA. pLenti Add to Cart
    42229 pX260-U6-DR-BB-DR-Cbh-NLS-hSpCas9-NLS-H1-shorttracr-PGK-puro humanized S. pyogenes Cas9 Mammalian Expression, CRISPR Multiplex Genome Engineering Using CRISPR/Cas Systems. Science. 2013 Jan 3. This plasmid separately encodes a human codon-optimized SpCas9, a tracrRNA and customizable crRNA. pUC ori vector Add to Cart
    42230 pX330-U6-Chimeric_BB-CBh-hSpCas9 humanized S. pyogenes Cas9 Mammalian Expression, CRISPR Multiplex Genome Engineering Using CRISPR/Cas Systems. Science. 2013 Jan 3. A human codon-optimized SpCas9 and chimeric guide RNA expression plasmid. pUC ori vector
  • Tag / Fusion Protein
    • 3xFLAG (N terminal on insert)
  • Add to Cart
    42333 pX334-U6-DR-BB-DR-Cbh-NLS-hSpCas9n(D10A)-NLS-H1-shorttracr-PGK-puro humanized S. pyogenes Cas9 (D10A) nickase Mammalian Expression, CRISPR Multiplex Genome Engineering Using CRISPR/Cas Systems. Science. 2013 Jan 3. This plasmid separately encodes a human codon-optimized SpCas9 nickase, a tracrRNA and customizable crRNA. pUC ori vector
  • Tag / Fusion Protein
    • HA (N terminal on insert)
  • Add to Cart
    42335 pX335-U6-Chimeric_BB-CBh-hSpCas9n(D10A) humanized S. pyogenes Cas9 (D10A) nickase Mammalian Expression, CRISPR Multiplex Genome Engineering Using CRISPR/Cas Systems. Science. 2013 Jan 3. A human codon-optimized SpCas9 nickase and chimeric guide RNA expression plasmid. pUC ori vector
  • Tag / Fusion Protein
    • HA (N terminal on insert)
  • Add to Cart
    42337 pX261-U6-DR-hEmx1-DR-Cbh-NLS-hSpCas9-NLS-H1-shorttracr-PGK-puro humanized S. pyogenes Cas9 Mammalian Expression, CRISPR Multiplex Genome Engineering Using CRISPR/Cas Systems. Science. 2013 Jan 3. Dual expression plasmid of human codon-optimized SpCas9 and a gRNA to the human Emx1 locus, can be used to test SpCas9 cleavage in cell lines of choice. pUC ori vector
  • Tag / Fusion Protein
    • 3xFLAG (N terminal on insert)
  • Add to Cart
    42581 pAAV_TALE-TF(VP64)-BB_V3 TALE-TF(VP64)-BB Mammalian Expression, AAV ; Adeno-associated Virus () Comprehensive interrogation of natural TALE DNA-binding modules and transcriptional repressor domains. Nat Commun. 2012 Jul 24;3:968. doi: 10.1038/ncomms1962. pAAV Add to Cart
    42588 pAAV_TALEN-FokI(WT)-BB_V3 TALEN-FokI(WT)-BB Mammalian Expression, AAV ; Adeno-associated Virus () Comprehensive interrogation of natural TALE DNA-binding modules and transcriptional repressor domains. Nat Commun. 2012 Jul 24;3:968. doi: 10.1038/ncomms1962. pAAV
  • Tag / Fusion Protein
    • 3XFlag (N terminal on backbone)
  • Add to Cart
    42945 SOX2 TALE Repressor (KRAB 1-75) SOX2 TALE Repressor (KRAB 1-75) Mammalian Expression, Lentiviral Comprehensive interrogation of natural TALE DNA-binding modules and transcriptional repressor domains. Nat Commun. 2012 Jul 24;3:968. doi: 10.1038/ncomms1962. pLenti
  • Tag / Fusion Protein
    • 2A-GFP (C terminal on insert)
  • Add to Cart
    42946 SOX2 TALE Repressor (KRAB 11-75) SOX2 TALE Repressor (KRAB 11-75) Mammalian Expression, Lentiviral Comprehensive interrogation of natural TALE DNA-binding modules and transcriptional repressor domains. Nat Commun. 2012 Jul 24;3:968. doi: 10.1038/ncomms1962. pLenti
  • Tag / Fusion Protein
    • 2A-GFP (C terminal on insert)
  • Add to Cart
    43882 Enhanced Repressor Domain for TAL Effector (SID4X) SID4X Domain Comprehensive interrogation of natural TALE DNA-binding modules and transcriptional repressor domains. Nat Commun. 2012 Jul 24;3:968. doi: 10.1038/ncomms1962. pUC57 Add to Cart
    47445 pAAV_hSyn_TALEBB(NN)-NLS-VP64_2A_GFP_WPRE_bGHpA TALE BB(N136_0.5NN_C63)-VP64 (Synthetic) Mammalian Expression, AAV ; TALE Optical control of mammalian endogenous transcription and epigenetic states. Nature. 2013 Jul 23. doi: 10.1038/nature12466. AAV TALE synthesis backbone with NN half repeat; fused to nuclear localization sequence linker and VP64 transcriptional activator effector domain. Synapsin promoter for neuronal expression. pAAV_hSyn_WPRE_bGHpA Add to Cart
    47446 pAAV_hSyn_TALEBB(HD)-NLS-VP64_2A_GFP_WPRE_bGHpA TALE BB(N136_0.5HD_C63)-VP64 (Synthetic) Mammalian Expression, AAV ; TALE Optical control of mammalian endogenous transcription and epigenetic states. Nature. 2013 Jul 23. doi: 10.1038/nature12466. AAV TALE synthesis backbone with HD half repeat; fused to nuclear localization sequence linker and VP64 transcriptional activator effector domain. Synapsin promoter for neuronal expression. pAAV_hSyn_WPRE_bGHpA Add to Cart
    47447 pAAV_hSyn_TALEBB(NG)-NLS-VP64_2A_GFP_WPRE_bGHpA TALE BB(N136_0.5NG_C63)-VP64 (Synthetic) Mammalian Expression, AAV ; TALE Optical control of mammalian endogenous transcription and epigenetic states. Nature. 2013 Jul 23. doi: 10.1038/nature12466. AAV TALE synthesis backbone with NG half repeat; fused to nuclear localization sequence linker and VP64 transcriptional activator effector domain. Synapsin promoter for neuronal expression. pAAV_hSyn_WPRE_bGHpA Add to Cart
    47448 pAAV_hSyn_TALEBB(NI)-NLS-VP64_2A_GFP_WPRE_bGHpA TALE BB(N136_0.5NI_C63)-VP64 (Synthetic) Mammalian Expression, AAV ; TALE Optical control of mammalian endogenous transcription and epigenetic states. Nature. 2013 Jul 23. doi: 10.1038/nature12466. AAV TALE synthesis backbone with NI half repeat; fused to nuclear localization sequence linker and VP64 transcriptional activator effector domain. Synapsin promoter for neuronal expression. pAAV_hSyn_WPRE_bGHpA Add to Cart
    47449 pAAV_hSyn_TALEBB(NN)-NLS-SID4X_2A_phiLOV2.1_WPRE_bGHpA TALE BB(N136_0.5NN_C63)-SID4X (Synthetic) Mammalian Expression, AAV ; TALE Optical control of mammalian endogenous transcription and epigenetic states. Nature. 2013 Jul 23. doi: 10.1038/nature12466. AAV TALE synthesis backbone with NN half repeat; fused to nuclear localization sequence linker and SID4X transcriptional repressor effector domain. Synapsin promoter for neuronal expression. pAAV_hSyn_WPRE_bGHpA Add to Cart
    47450 pAAV_hSyn_TALEBB(HD)-NLS-SID4X_2A_phiLOV2.1_WPRE_bGHpA TALE BB(N136_0.5HD_C63)-SID4X (Synthetic) Mammalian Expression, AAV ; TALE Optical control of mammalian endogenous transcription and epigenetic states. Nature. 2013 Jul 23. doi: 10.1038/nature12466. AAV TALE synthesis backbone with HD half repeat; fused to nuclear localization sequence linker and SID4X transcriptional repressor effector domain. Synapsin promoter for neuronal expression. pAAV_hSyn_WPRE_bGHpA Add to Cart
    47451 pAAV_hSyn_TALEBB(NG)-NLS-SID4X_2A_phiLOV2.1_WPRE_bGHpA TALE BB(N136_0.5NG_C63)-SID4X (Synthetic) Mammalian Expression, AAV ; TALE Optical control of mammalian endogenous transcription and epigenetic states. Nature. 2013 Jul 23. doi: 10.1038/nature12466. AAV TALE synthesis backbone with NG half repeat; fused to nuclear localization sequence linker and SID4X transcriptional repressor effector domain. Synapsin promoter for neuronal expression. pAAV_hSyn_WPRE_bGHpA Add to Cart
    47452 pAAV_hSyn_TALEBB(NI)-NLS-SID4X_2A_phiLOV2.1_WPRE_bGHpA TALE BB(N136_0.5NI_C63)-SID4X (Synthetic) Mammalian Expression, AAV ; TALE Optical control of mammalian endogenous transcription and epigenetic states. Nature. 2013 Jul 23. doi: 10.1038/nature12466. AAV TALE synthesis backbone with NI half repeat; fused to nuclear localization sequence linker and SID4X transcriptional repressor effector domain. Synapsin promoter for neuronal expression. pAAV_hSyn_WPRE_bGHpA Add to Cart
    47453 LITE1.0_pAAV_hSyn_TALE(Grm2)-NLS-CIB1_2A_GFP_WPRE_bGHpA TALE (N136,Grm2,C63)-cib1 (Synthetic) Mammalian Expression, AAV ; TALE Optical control of mammalian endogenous transcription and epigenetic states. Nature. 2013 Jul 23. doi: 10.1038/nature12466. epiLITE / LITE1.0 TALE-CIB1. CIB1 binds to blue-light-activated CRY2PHR. Particular TALE targeted to Grm2 promoter. Synapsin promoter for neuronal expression. pAAV_hSyn_WPRE_bGHpA Add to Cart
    47454 LITE1.0_pAAV_hSyn_CRY2PHR-NLS-SID4X_2A_phiLOV2.1_WPRE_bGHpA CRY2PHR-NLS-SID4X (Synthetic) Mammalian Expression, AAV Optical control of mammalian endogenous transcription and epigenetic states. Nature. 2013 Jul 23. doi: 10.1038/nature12466. epiLITE / LITE1.0 CRY2PHR fused to SID4X transcriptional repressor effector domain. Binds to CIB1 upon blue light stimulation. Synapsin promoter for neuronal expression. pAAV_hSyn_WPRE_bGHpA Add to Cart
    47455 LITE2.0 pAAV_hSyn_NLS(alpha-imp)-CRY2PHR-NLS-VP64_2A_GFP_WPRE_bGHpA NLS(alpha-imp)-CRY2PHR-NLS-VP64 (Synthetic) Mammalian Expression, AAV Optical control of mammalian endogenous transcription and epigenetic states. Nature. 2013 Jul 23. doi: 10.1038/nature12466. LITE2.0 CRY2PHR-VP64. Changes from LITE1.0: N-terminal alpha-importin nuclear localization sequence. Synapsin promoter for neuronal expression. pAAV_hSyn_WPRE_bGHpA Add to Cart
    47456 LITE2.0_pAAV_hSyn_TALE(Grm2)-GS-CIB1(NLS*,∆318-334)_WPRE_bGHpA TALE(Grm2)-GS-CIB1(NLS*,∆318-334) (Synthetic) Mammalian Expression, AAV ; TALE Optical control of mammalian endogenous transcription and epigenetic states. Nature. 2013 Jul 23. doi: 10.1038/nature12466. LITE2.0 TALE-CIB1. Changes from LITE1.0: glycine-serine linker, mutated endogenous NLS, aa318-334 deletion. This particular TALE targeted to Grm2. Synapsin promoter for neuronal expression. pAAV_hSyn_WPRE_bGHpA Add to Cart
    47457 LITE2.0 EF1a_NLS(alpha-imp)-CRY2PHR-NLS-VP64_2A_GFP_WPRE_hGHpA NLS(alpha-imp)-CRY2PHR-NLS-VP64 (Synthetic) Mammalian Expression Optical control of mammalian endogenous transcription and epigenetic states. Nature. 2013 Jul 23. doi: 10.1038/nature12466. LITE2.0 CRY2PHR-VP64. Changes from LITE1.0: N-terminal alpha-importin nuclear localization sequence. EF1-alpha promoter for ubiquitous mammalian expression. pAAV_EF1a_WPRE_hGHpA Add to Cart
    47458 LITE2.0 EF1a_TALE(Neurog2)-GS-CIB1(NLS*,∆318-334)_WPRE_hGHpA TALE (Neurog2)-GS-CIB1(NLS*,∆318-334) (Synthetic) Mammalian Expression Optical control of mammalian endogenous transcription and epigenetic states. Nature. 2013 Jul 23. doi: 10.1038/nature12466. LITE2.0 TALE-CIB1. Changes from LITE1.0: glycine-serine linker, mutated endogenous NLS, aa318-334 deletion. This particular TALE targeted to Neurog2. EF1-alpha promoter for ubiquitous mammalian exp. pAAV_EF1a_WPRE_hGHpA Add to Cart
    48137 pSpCas9 (PX165) hSpCas9 (Synthetic) Mammalian Expression, CRISPR Genome engineering using the CRISPR-Cas9 system. Nat Protoc. 2013 Nov;8(11):2281-308. doi: 10.1038/nprot.2013.143. Epub 2013 Oct 24. Human codon optimized Cas9 nuclease from Streptococcus pyogenes (Cbh-3X-FLAG-NLS-SpCas9-NLS) PX165 Add to Cart
    48138 pSpCas9(BB)-2A-GFP (PX458) hSpCas9 (Synthetic) Mammalian Expression, CRISPR Genome engineering using the CRISPR-Cas9 system. Nat Protoc. 2013 Nov;8(11):2281-308. doi: 10.1038/nprot.2013.143. Epub 2013 Oct 24. Cas9 from S. pyogenes with 2A-EGFP, and cloning backbone for sgRNA PX458 Add to Cart
    48139 pSpCas9(BB)-2A-Puro (PX459) hSpCas9-2A-Puro (Synthetic) Mammalian Expression, CRISPR Genome engineering using the CRISPR-Cas9 system. Nat Protoc. 2013 Nov;8(11):2281-308. doi: 10.1038/nprot.2013.143. Epub 2013 Oct 24. NOTE: A new version of this plasmid is now available. See Addgene plasmid 62988. PX459 Add to Cart
    48140 pSpCas9n(BB)-2A-GFP (PX461) hSpCas9n (Synthetic) Mammalian Expression, CRISPR Genome engineering using the CRISPR-Cas9 system. Nat Protoc. 2013 Nov;8(11):2281-308. doi: 10.1038/nprot.2013.143. Epub 2013 Oct 24. Cas9n (D10A nickase mutant) from S. pyogenes with 2A-EGFP, and cloning backbone for sgRNA PX461 Add to Cart
    48141 pSpCas9n(BB)-2A-Puro (PX462) hSpCas9n-2A-Puro (Synthetic) Mammalian Expression, CRISPR Genome engineering using the CRISPR-Cas9 system. Nat Protoc. 2013 Nov;8(11):2281-308. doi: 10.1038/nprot.2013.143. Epub 2013 Oct 24. NOTE: A new version of this plasmid is now available. See Addgene plasmid 62987 PX462 Add to Cart
    48253 LITE1.0_pAAV_hSyn_CRY2PHR-NLS-VP64_2A_GFP_WPRE_bGHpA CRY2PHR-NLS-VP64 (Synthetic) Mammalian Expression, AAV ; TALE Optical control of mammalian endogenous transcription and epigenetic states. Nature. 2013 Jul 23. doi: 10.1038/nature12466. LITE1.0 CRY2PHR fused to VP64 transcriptional activator domain. Binds to CIB1 upon blue light stimulation. Synapsin promoter for neuronal expression. See LITE2.0 for optimized LITE activators. pAAV_hSyn_WPRE_bGHpA Add to Cart
    48873 pSpCas9n(BB) (PX460) hSpCas9n (Synthetic) Mammalian Expression, CRISPR Genome engineering using the CRISPR-Cas9 system. Nat Protoc. 2013 Nov;8(11):2281-308. doi: 10.1038/nprot.2013.143. Epub 2013 Oct 24. Cas9n (D10A nickase mutant) from S. pyogenes PX460 Add to Cart
    51760 lentiCRISPR - EGFP sgRNA 1 Cas9 (Synthetic), Puromycin resistance (Other), EGFP sgRNA 1 (Synthetic) Mammalian Expression, Lentiviral, CRISPR Genome-scale CRISPR-Cas9 knockout screening in human cells. Science. 2014 Jan 3;343(6166):84-7. doi: 10.1126/science.1247005. Epub 2013 Dec 12. Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an EGFP targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone. lentiCRISPR (pXPR_001), Addgene plasmid #49535 Add to Cart
    51761 lentiCRISPR - EGFP sgRNA 2 Cas9 (Synthetic), Puromycin resistance (Other), EGFP sgRNA 2 (Synthetic) Mammalian Expression, Lentiviral, CRISPR Genome-scale CRISPR-Cas9 knockout screening in human cells. Science. 2014 Jan 3;343(6166):84-7. doi: 10.1126/science.1247005. Epub 2013 Dec 12. Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an EGFP targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone. lentiCRISPR (pXPR_001), Addgene plasmid #49535 Add to Cart
    51762 lentiCRISPR - EGFP sgRNA 3 Cas9 (Synthetic), Puromycin resistance (Other), EGFP sgRNA 3 (Synthetic) Mammalian Expression, Lentiviral, CRISPR Genome-scale CRISPR-Cas9 knockout screening in human cells. Science. 2014 Jan 3;343(6166):84-7. doi: 10.1126/science.1247005. Epub 2013 Dec 12. Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an EGFP targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone. lentiCRISPR (pXPR_001), Addgene plasmid #49535 Add to Cart
    51763 lentiCRISPR - EGFP sgRNA 4 Cas9 (Synthetic), Puromycin resistance (Other), EGFP sgRNA 4 (Synthetic) Mammalian Expression, Lentiviral, CRISPR Genome-scale CRISPR-Cas9 knockout screening in human cells. Science. 2014 Jan 3;343(6166):84-7. doi: 10.1126/science.1247005. Epub 2013 Dec 12. Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an EGFP targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone. lentiCRISPR (pXPR_001), Addgene plasmid #49535 Add to Cart
    51764 lentiCRISPR - EGFP sgRNA 5 Cas9 (Synthetic), Puromycin resistance (Other), EGFP sgRNA 5 (Synthetic) Lentiviral, CRISPR Genome-scale CRISPR-Cas9 knockout screening in human cells. Science. 2014 Jan 3;343(6166):84-7. doi: 10.1126/science.1247005. Epub 2013 Dec 12. Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an EGFP targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone. lentiCRISPR (pXPR_001), Addgene plasmid #49535 Add to Cart
    51765 lentiCRISPR - EGFP sgRNA 6 Cas9 (Synthetic), Puromycin resistance (Other), EGFP sgRNA 6 (Synthetic) Mammalian Expression, Lentiviral, CRISPR Genome-scale CRISPR-Cas9 knockout screening in human cells. Science. 2014 Jan 3;343(6166):84-7. doi: 10.1126/science.1247005. Epub 2013 Dec 12. Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an EGFP targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone. lentiCRISPR (pXPR_001), Addgene plasmid #49535 Add to Cart
    52961 lentiCRISPR v2 Cas9 (Synthetic), Puromycin resistance Mammalian Expression, Lentiviral, CRISPR Improved vectors and genome-wide libraries for CRISPR screening. Nat Methods. 2014 Aug;11(8):783-4. doi: 10.1038/nmeth.3047. Replaces original lentiCRISPRv1 (Addgene Plasmid 49535) and produces ~10-fold higher titer virus. Custom Add to Cart
    52962 lentiCas9-Blast Cas9 (Synthetic), Blasticidin resistance Mammalian Expression, Lentiviral, CRISPR Improved vectors and genome-wide libraries for CRISPR screening. Nat Methods. 2014 Aug;11(8):783-4. doi: 10.1038/nmeth.3047. Expresses human codon-optimized S. pyogenes Cas9 protein and blasticidin resistance from EFS promoter. Lentiviral backbone. pFUGW Add to Cart
    52963 lentiGuide-Puro S. pyogenes sgRNA cassette (Synthetic), Puromycin resistance Mammalian Expression, Lentiviral, CRISPR Improved vectors and genome-wide libraries for CRISPR screening. Nat Methods. 2014 Aug;11(8):783-4. doi: 10.1038/nmeth.3047. Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and puromycin resistance from EF-1a promoter. Lentiviral backbone. Custom Add to Cart
    58888 PX543 SpCas9(del175-307) hSpCas9 (Synthetic) Mammalian Expression, CRISPR Crystal structure of Cas9 in complex with guide RNA and target DNA. Cell. 2014 Feb 27;156(5):935-49. doi: 10.1016/j.cell.2014.02.001. Epub 2014 Feb 13. Truncated form of SpCas9 without REC2 domain PX165 Add to Cart
    58889 PX544 SpCas9(del1098-1368) hSpCas9 (Synthetic) Mammalian Expression, CRISPR Crystal structure of Cas9 in complex with guide RNA and target DNA. Cell. 2014 Feb 27;156(5):935-49. doi: 10.1016/j.cell.2014.02.001. Epub 2014 Feb 13. Truncated form of SpCas9 without C' domain PX165 Add to Cart
    60224 AAV:ITR-U6-sgRNA(Kras)-U6-sgRNA(p53)-U6-sgRNA(Lkb1)-pEFS-Rluc-2A-Cre-shortPA-KrasG12D_HDRdonor-ITR (AAV-KPL) sgRNA (Synthetic), Renilla luciferase, Cre recombinase, KrasG12D HDR donor Mammalian Expression, Mouse Targeting, AAV, Cre/Lox, CRISPR CRISPR-Cas9 Knockin Mice for Genome Editing and Cancer Modeling. Cell. 2014 Sep 24. pii: S0092-8674(14)01163-5. doi: 10.1016/j.cell.2014.09.014. Expresses Cre recombinase and Renilla luciferase from the EFS promoter and three U6-driven sgRNAs targeting Kras, p53, and Lkb1. Contains KrasG12D HDR donor. AAV backbone. AAV Add to Cart
    60225 AAV:ITR-U6-sgRNA(LacZ)-pEFS-Rluc-2A-Cre-WPRE-hGHpA-ITR sgRNA, Renilla luciferase, Cre recombinase Mammalian Expression, Mouse Targeting, AAV, Cre/Lox, CRISPR, Luciferase CRISPR-Cas9 Knockin Mice for Genome Editing and Cancer Modeling. Cell. 2014 Sep 24. pii: S0092-8674(14)01163-5. doi: 10.1016/j.cell.2014.09.014. Expresses Cre recombinase and Renilla luciferase from the EFS promoter and one U6-driven sgRNA targeting LacZ. AAV backbone. AAV Add to Cart
    60226 AAV:ITR-U6-sgRNA(backbone)-pEFS-Rluc-2A-Cre-WPRE-hGHpA-ITR sgRNA, Renilla luciferase, Cre recombinase Mammalian Expression, Mouse Targeting, AAV, Cre/Lox, CRISPR, Luciferase CRISPR-Cas9 Knockin Mice for Genome Editing and Cancer Modeling. Cell. 2014 Sep 24. pii: S0092-8674(14)01163-5. doi: 10.1016/j.cell.2014.09.014. Expresses Cre recombinase and Renilla luciferase from the EFS promoter and one U6-driven sgRNA. AAV backbone with SapI spacer for sgRNA cloning. AAV Add to Cart
    60227 AAV:ITR-U6-sgRNA(NeuN)-pCBh-Cre-WPRE-hGHpA-ITR sgRNA, Cre recombinase Mammalian Expression, Mouse Targeting, AAV, Cre/Lox, CRISPR CRISPR-Cas9 Knockin Mice for Genome Editing and Cancer Modeling. Cell. 2014 Sep 24. pii: S0092-8674(14)01163-5. doi: 10.1016/j.cell.2014.09.014. Expresses Cre recombinase from the Cbh promoter and one U6-driven sgRNA targeting the mouse gene NeuN. AAV backbone. AAV Add to Cart
    60228 AAV:ITR-U6-sgRNA(LacZ)-pCBh-Cre-WPRE-hGHpA-ITR sgRNA, Cre recombinase Mammalian Expression, Mouse Targeting, AAV, Cre/Lox, CRISPR CRISPR-Cas9 Knockin Mice for Genome Editing and Cancer Modeling. Cell. 2014 Sep 24. pii: S0092-8674(14)01163-5. doi: 10.1016/j.cell.2014.09.014. Expresses Cre recombinase from the Cbh promoter and one U6-driven sgRNA control targeting LacZ. AAV backbone. AAV Add to Cart
    60229 AAV:ITR-U6-sgRNA(backbone)-pCBh-Cre-WPRE-hGHpA-ITR sgRNA, Cre recombinase Mammalian Expression, Mouse Targeting, AAV, Cre/Lox, CRISPR CRISPR-Cas9 Knockin Mice for Genome Editing and Cancer Modeling. Cell. 2014 Sep 24. pii: S0092-8674(14)01163-5. doi: 10.1016/j.cell.2014.09.014. Expresses Cre recombinase from the Cbh promoter and one U6-driven sgRNA. AAV backbone with SapI spacer for sgRNA cloning. AAV Add to Cart
    60230 AAV:ITR-U6-sgRNA(NeuN)-hSyn-Cre-2A-EGFP-KASH-WPRE-shortPA-ITR sgRNA, Cre recombinase, EGFP Mammalian Expression, Mouse Targeting, AAV, Cre/Lox, CRISPR CRISPR-Cas9 Knockin Mice for Genome Editing and Cancer Modeling. Cell. 2014 Sep 24. pii: S0092-8674(14)01163-5. doi: 10.1016/j.cell.2014.09.014. Expresses Cre recombinase and KASH-tagged EGFP from the hSyn promoter and one U6-driven sgRNA. AAV backbone with SapI spacer for sgRNA cloning. AAV Add to Cart
    60231 AAV:ITR-U6-sgRNA(backbone)-hSyn-Cre-2A-EGFP-KASH-WPRE-shortPA-ITR sgRNA, Cre recombinase, EGFP Mammalian Expression, Mouse Targeting, AAV, Cre/Lox, CRISPR CRISPR-Cas9 Knockin Mice for Genome Editing and Cancer Modeling. Cell. 2014 Sep 24. pii: S0092-8674(14)01163-5. doi: 10.1016/j.cell.2014.09.014. Expresses Cre recombinase and KASH-tagged EGFP from the hSyn promoter and one U6-driven sgRNA. AAV backbone with SapI spacer for sgRNA cloning. AAV Add to Cart
    60957 PX551 SpCas9 (Synthetic) Mammalian Expression, AAV, CRISPR In vivo interrogation of gene function in the mammalian brain using CRISPR-Cas9. Nat Biotechnol. 2014 Oct 19. doi: 10.1038/nbt.3055. pAAV-pMecp2-SpCas9-spA (AAV-SpCas9). AAV plasmid expressing Cas9 in neurons under control of truncated mecp2 promoter. pAAV Add to Cart
    60958 PX552 U6_(SpaI)_sgRNA (Synthetic), Syn_EGFP-KASH (Synthetic) Mammalian Expression, Mouse Targeting, AAV, CRISPR In vivo interrogation of gene function in the mammalian brain using CRISPR-Cas9. Nat Biotechnol. 2014 Oct 19. doi: 10.1038/nbt.3055. pAAV-U6sgRNA(SapI)_hSyn-GFP-KASH-bGH (SpGuide acceptor). AAV plasmid for sgRNA cloning. GFP-KASH fusion facilitates FACS sorting of cells and nuclei. pAAV Add to Cart
    61408 LSL-Cas9-Rosa26TV Cas9 (Synthetic), EGFP Mammalian Expression, Mouse Targeting, Cre/Lox, CRISPR CRISPR-Cas9 Knockin Mice for Genome Editing and Cancer Modeling. Cell. 2014 Sep 24. pii: S0092-8674(14)01163-5. doi: 10.1016/j.cell.2014.09.014. Cre-dependent Cas9 expression plasmid and targeting vector for the mouse Rosa26 locus. Cas9 expression is Cre-dependent and the targeting vector has positive (Neo) and negative (DTA) selection. Ai9 Add to Cart
    61422 dCAS9-VP64_GFP dCAS9(D10A,H840A)-VP64_2A_GFP (Synthetic) Mammalian Expression, Lentiviral, CRISPR Genome-scale transcriptional activation by an engineered CRISPR-Cas9 complex. Nature. 2014 Dec 10. doi: 10.1038/nature14136. Expresses dCAS9-VP64 activator with 2A GFP lenti(AMP) Add to Cart
    61423 MS2-P65-HSF1_GFP MS2(N55K)-P65-HSF1_2A_GFP (Homo sapiens) Mammalian Expression, CRISPR Genome-scale transcriptional activation by an engineered CRISPR-Cas9 complex. Nature. 2014 Dec 10. doi: 10.1038/nature14136. Expresses the MS2-P65-HSF1 activator helper complex with a 2A GFP lenti(AMP) Add to Cart
    61424 sgRNA(MS2) cloning backbone Mammalian Expression, CRISPR Genome-scale transcriptional activation by an engineered CRISPR-Cas9 complex. Nature. 2014 Dec 10. doi: 10.1038/nature14136. sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2. Contains BbsI sites for insertion of spacer sequences. pUC19 Add to Cart
    61425 lenti dCAS-VP64_Blast dCAS9(D10A, N863A)-VP64_2A_Blast (Synthetic) Mammalian Expression, Lentiviral, CRISPR Genome-scale transcriptional activation by an engineered CRISPR-Cas9 complex. Nature. 2014 Dec 10. doi: 10.1038/nature14136. lenti vector encoding dCAS9-VP64 with 2A Blast resistance marker (EF1a-NLS-dCas9(N863)-VP64-2A-Blast-WPRE) plenti Add to Cart
    61426 lenti MS2-P65-HSF1_Hygro MS2-P65-HSF1_2A_Hygro (Homo sapiens) Mammalian Expression, Lentiviral, CRISPR Genome-scale transcriptional activation by an engineered CRISPR-Cas9 complex. Nature. 2014 Dec 10. doi: 10.1038/nature14136. lenti vector encoding the MS2-P65-HSF1 activator helper complex with a 2A Hygro resistance marker (EF1a-MS2-p65-HSF1-2A-Hygro-WPRE) plenti Add to Cart
    61427 lenti sgRNA(MS2)_zeo backbone Mammalian Expression, Lentiviral, CRISPR Genome-scale transcriptional activation by an engineered CRISPR-Cas9 complex. Nature. 2014 Dec 10. doi: 10.1038/nature14136. lenti sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2 and EF1a-zeo resistance marker. Contains BsmBI sites for insertion of spacer sequences. plenti Add to Cart
    61591 pX601-AAV-CMV::NLS-SaCas9-NLS-3xHA-bGHpA;U6::BsaI-sgRNA hSaCas9 (Other), Chimeric guide for SaCas9 (Other) Mammalian Expression, AAV, CRISPR In vivo genome editing using Staphylococcus aureus Cas9. Nature. 2015 Apr 1. doi: 10.1038/nature14299. A single vector AAV-Cas9 system containing Cas9 from Staphylococcus aureus (SaCas9) and its sgRNA. pAAV Add to Cart
    61592 pX600-AAV-CMV::NLS-SaCas9-NLS-3xHA-bGHpA hSaCas9 (Other) Mammalian Expression, AAV, CRISPR In vivo genome editing using Staphylococcus aureus Cas9. Nature. 2015 Apr 1. doi: 10.1038/nature14299. A human codon-optimized Cas9 from Staphylococcus aureus (SaCas9). pAAV Add to Cart
    61593 pX602-AAV-TBG::NLS-SaCas9-NLS-HA-OLLAS-bGHpA;U6::BsaI-sgRNA hSaCas9 (Other), Chimeric guide for SaCas9 (Other) Mammalian Expression, AAV, CRISPR In vivo genome editing using Staphylococcus aureus Cas9. Nature. 2015 Apr 1. doi: 10.1038/nature14299. A single vector AAV-Cas9 system containing Cas9 from Staphylococcus aureus (SaCas9) and its sgRNA. pAAV Add to Cart
    61594 pX603-AAV-CMV::NLS-dSaCas9(D10A,N580A)-NLS-3xHA-bGHpA hSaCas9 (Other) Mammalian Expression, AAV, CRISPR In vivo genome editing using Staphylococcus aureus Cas9. Nature. 2015 Apr 1. doi: 10.1038/nature14299. A catalytically inactive SaCas9 (dSaCas9) with the D10A and N580A mutations. pAAV Add to Cart
    61597 Human SAM library Human SAM library (Synthetic) Mammalian Expression, Lentiviral, CRISPR Zhang SAM library (unpublished) SAM can be combined with a human genome-wide library to activate all known coding isoforms from the RefSeq database (23,430 isoforms). lenti sgRNA(MS2)_zeo backbone (Addgene plasmid 61427) Add to Cart
    62883 PX851 SpCas9 (aa 2-535) Mammalian Expression, CRISPR A split-Cas9 architecture for inducible genome editing and transcription modulation. Nat Biotechnol. 2015 Feb 2. doi: 10.1038/nbt.3149. N-term SpCas9 piece of inducible split-4 (Cas9(N)-FRB-NES split-4). PX330 Add to Cart
    62884 PX852 SpCas9 (aa536-1368) Mammalian Expression, CRISPR A split-Cas9 architecture for inducible genome editing and transcription modulation. Nat Biotechnol. 2015 Feb 2. doi: 10.1038/nbt.3149. C-term SpCas9 piece of inducible split-4 (Cas9(C)-FKBP split-4). PX330 Add to Cart
    62885 PX853 SpCas9 (aa 2-573) Mammalian Expression, CRISPR A split-Cas9 architecture for inducible genome editing and transcription modulation. Nat Biotechnol. 2015 Feb 2. doi: 10.1038/nbt.3149. N-term SpCas9 piece of inducible split-5 (Cas9(N)-FRB-NES split-5). PX330 Add to Cart
    62886 PX854 SpCas9 (aa573-1368) Mammalian Expression, CRISPR A split-Cas9 architecture for inducible genome editing and transcription modulation. Nat Biotechnol. 2015 Feb 2. doi: 10.1038/nbt.3149. C-term SpCas9 piece of inducible split-5 (Cas9(C)-FKBP split-5). PX330 Add to Cart
    62887 PX855 SpCas9 (aa 2-535) Mammalian Expression, CRISPR A split-Cas9 architecture for inducible genome editing and transcription modulation. Nat Biotechnol. 2015 Feb 2. doi: 10.1038/nbt.3149. N-term SpCas9 piece of inducible transcriptional activator (dCas9(N)-FRB-2xNES) PX330 Add to Cart
    62888 PX856 SpCas9 (aa536-1368) Mammalian Expression, CRISPR A split-Cas9 architecture for inducible genome editing and transcription modulation. Nat Biotechnol. 2015 Feb 2. doi: 10.1038/nbt.3149. C-term SpCas9 piece of inducible transcriptional activator (dCas9(C)-FKBP-2xNLS-VP64) PX330 Add to Cart
    62889 pLSC-5 SpCas9(574-1368), SpCas9(2-573) Mammalian Expression, Lentiviral, CRISPR A split-Cas9 architecture for inducible genome editing and transcription modulation. Nat Biotechnol. 2015 Feb 2. doi: 10.1038/nbt.3149. Lenti vector for expression of inducible split Cas9 lentiCRISPR Add to Cart
    62987 pSpCas9n(BB)-2A-Puro (PX462) V2.0 hSpCas9n-2A-Puro (Synthetic) Mammalian Expression, CRISPR Genome engineering using the CRISPR-Cas9 system. Nat Protoc. 2013 Nov;8(11):2281-308. doi: 10.1038/nprot.2013.143. Epub 2013 Oct 24. Cas9n (D10A nickase mutant) from S. pyogenes with 2A-Puro, and cloning backbone for sgRNA (V2.0) PX462 Add to Cart
    62988 pSpCas9(BB)-2A-Puro (PX459) V2.0 hSpCas9-2A-Puro V2.0 (Synthetic) Mammalian Expression, CRISPR Genome engineering using the CRISPR-Cas9 system. Nat Protoc. 2013 Nov;8(11):2281-308. doi: 10.1038/nprot.2013.143. Epub 2013 Oct 24. Cas9 from S. pyogenes with 2A-Puro, and cloning backbone for sgRNA (V2.0) PX459 Add to Cart
    63592 lentiCas9-EGFP Cas9 (Synthetic), EGFP (Other) Mammalian Expression, Lentiviral, CRISPR Genome-wide CRISPR screen in a mouse model of tumor growth and metastasis. Cell. 2015 Mar 12;160(6):1246-60. doi: 10.1016/j.cell.2015.02.038. Epub 2015 Mar 5. Expresses human codon-optimized S. pyogenes Cas9 protein and EGFP from EFS promoter. Lentiviral backbone. pFUGW Add to Cart
    63593 lentiCas9n(D10A)-Blast Cas9 (Synthetic), Blasticidin resistance Mammalian Expression, Lentiviral, CRISPR Improved vectors and genome-wide libraries for CRISPR screening. Nat Methods. 2014 Aug;11(8):783-4. doi: 10.1038/nmeth.3047. Expresses human codon-optimized S. pyogenes Cas9n (D10A nickase) protein and blasticidin resistance from EFS promoter. Lentiviral backbone. pFUGW Add to Cart
    68315 PX378 Sutterella wadsworthensis Cas9 SwCas9 (Synthetic) Mammalian Expression, CRISPR PX377 Corynebacter diphtheriae (unpublished) Sutterella wadsworthensis Cas9 pDREAM Add to Cart
    68317 PX380 Treponema denticola Cas9 TdCas9 (Synthetic) Mammalian Expression, CRISPR PX377 Corynebacter diphtheriae (unpublished) Treponema denticola Cas9 pDREAM Add to Cart
    68318 PX381 Filifactor alocis Cas9 FaCas9 (Synthetic) Mammalian Expression, CRISPR PX377 Corynebacter diphtheriae (unpublished) Filifactor alocis Cas9 pDREAM Add to Cart
    68319 PX382 Staphylococcus pseudintermedius Cas9 SpsCas9 (Synthetic) Mammalian Expression, CRISPR PX377 Corynebacter diphtheriae (unpublished) Staphylococcus pseudintermedius Cas9 pDREAM Add to Cart
    68322 PX385 Streptococcus pasteurianus Cas9 SpaCas9 (Synthetic) Mammalian Expression, CRISPR PX377 Corynebacter diphtheriae (unpublished) Streptococcus pasteurianus Cas9 pDREAM Add to Cart
    68323 PX386 Lactobacillus farciminis Cas9 LfCas9 (Synthetic) Mammalian Expression, CRISPR PX377 Corynebacter diphtheriae (unpublished) Lactobacillus farciminis Cas9 pDREAM Add to Cart
    68324 PX387 Mycoplasma mobile Cas9 MmCas9 (Synthetic) Mammalian Expression, CRISPR PX377 Corynebacter diphtheriae (unpublished) Mycoplasma mobile Cas9 pDREAM Add to Cart
    68327 PX390 Flavobacterium columnare Cas9 FcCas9 (Synthetic) Mammalian Expression, CRISPR PX377 Corynebacter diphtheriae (unpublished) Flavobacterium columnare Cas9 pDREAM Add to Cart
    68328 PX391 Sphaerochaeta globus str. Buddy Cas9 SgCas9 (Synthetic) Mammalian Expression, CRISPR PX377 Corynebacter diphtheriae (unpublished) Sphaerochaeta globus str. Buddy Cas9 pDREAM Add to Cart
    68329 PX392 Azospirillum B510 Cas9 AzoCas9 (Synthetic) Mammalian Expression, CRISPR PX377 Corynebacter diphtheriae (unpublished) Azospirillum B510 Cas9 pDREAM Add to Cart
    68330 PX393 Gluconacetobacter diazotrophicus Cas9 GdCas9 (Synthetic) Mammalian Expression, CRISPR PX377 Corynebacter diphtheriae (unpublished) Gluconacetobacter diazotrophicus Cas9 pDREAM Add to Cart
    68331 PX394 Neisseria cinerea Cas9 NcCas9 (Synthetic) Mammalian Expression, CRISPR PX377 Corynebacter diphtheriae (unpublished) Neisseria cinerea Cas9 pDREAM Add to Cart
    68332 PX395 Roseburia intestinalis Cas9 RiCas9 (Synthetic) Mammalian Expression, CRISPR PX377 Corynebacter diphtheriae (unpublished) Roseburia intestinalis Cas9 pDREAM Add to Cart
    68333 PX396 Parvibaculum lavamentivorans Cas9 PlCas9 (Synthetic) Mammalian Expression, CRISPR PX377 Corynebacter diphtheriae (unpublished) Parvibaculum lavamentivorans Cas9 pDREAM Add to Cart
    68334 PX398 Nitratifractor salsuginis str DSM 16511 Cas9 NsCas9 (Synthetic) Mammalian Expression, CRISPR PX377 Corynebacter diphtheriae (unpublished) Nitratifractor salsuginis str DSM 16511 Cas9 pDREAM Add to Cart
    68336 PX400 Campylobacter lari CF89-12 Cas9 ClCas9 (Synthetic) Mammalian Expression, CRISPR PX377 Corynebacter diphtheriae (unpublished) Campylobacter lari CF89-12 Cas9 pDREAM Add to Cart
    68702 PX405 Neisseria meningitidis Cas9 NmCas9 (Synthetic) Mammalian Expression, CRISPR PX405 Neisseria meningiditis Cas9 (unpublished) Neisseria meningitidis Cas9 pDREAM Add to Cart
    68703 PX406 Pasteurella multocida Cas9 PmCas9 (Synthetic) Mammalian Expression, CRISPR PX405 Neisseria meningiditis Cas9 (unpublished) Pasteurella multocida Cas9 pDREAM Add to Cart