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Feng Zhang Lab Plasmids


The Feng Zhang Lab has deposited plasmids at Addgene for distribution to the research community. Addgene is a non-profit plasmid repository dedicated to improving life science research.

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Title Authors Publication
Rationally engineered Cas9 nucleases with improved specificity. Slaymaker IM, Gao L, Zetsche B, Scott DA, Yan WX, Zhang F Science. 2015 Dec 1. pii: aad5227.
Discovery and Functional Characterization of Diverse Class 2 CRISPR-Cas Systems. Shmakov S, Abudayyeh OO, Makarova KS, Wolf YI, Gootenberg JS, Semenova E, Minakhin L, Joung J, Konermann S, Severinov K, Zhang F, Koonin EV Mol Cell. 2015 Nov 5;60(3):385-97. doi: 10.1016/j.molcel.2015.10.008. Epub 2015 Oct 22.
Cpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas System. Zetsche B, Gootenberg JS, Abudayyeh OO, Slaymaker IM, Makarova KS, Essletzbichler P, Volz SE, Joung J, van der Oost J, Regev A, Koonin EV, Zhang F Cell. 2015 Sep 23. pii: S0092-8674(15)01200-3. doi: 10.1016/j.cell.2015.09.038.
In vivo genome editing using Staphylococcus aureus Cas9. Ran FA, Cong L, Yan WX, Scott DA, Gootenberg JS, Kriz AJ, Zetsche B, Shalem O, Wu X, Makarova KS, Koonin EV, Sharp PA, Zhang F Nature. 2015 Apr 1. doi: 10.1038/nature14299.
Genome-wide CRISPR screen in a mouse model of tumor growth and metastasis. Chen S, Sanjana NE, Zheng K, Shalem O, Lee K, Shi X, Scott DA, Song J, Pan JQ, Weissleder R, Lee H, Zhang F, Sharp PA Cell. 2015 Mar 12;160(6):1246-60. doi: 10.1016/j.cell.2015.02.038. Epub 2015 Mar 5.
A split-Cas9 architecture for inducible genome editing and transcription modulation. Zetsche B, Volz SE, Zhang F Nat Biotechnol. 2015 Feb 2. doi: 10.1038/nbt.3149.
Genome-scale transcriptional activation by an engineered CRISPR-Cas9 complex. Konermann S, Brigham MD, Trevino AE, Joung J, Abudayyeh OO, Barcena C, Hsu PD, Habib N, Gootenberg JS, Nishimasu H, Nureki O, Zhang F Nature. 2014 Dec 10. doi: 10.1038/nature14136.
In vivo interrogation of gene function in the mammalian brain using CRISPR-Cas9. Swiech L, Heidenreich M, Banerjee A, Habib N, Li Y, Trombetta J, Sur M, Zhang F Nat Biotechnol. 2014 Oct 19. doi: 10.1038/nbt.3055.
CRISPR-Cas9 Knockin Mice for Genome Editing and Cancer Modeling. Platt RJ, Chen S, Zhou Y, Yim MJ, Swiech L, Kempton HR, Dahlman JE, Parnas O, Eisenhaure TM, Jovanovic M, Graham DB, Jhunjhunwala S, Heidenreich M, Xavier RJ, Langer R, Anderson DG, Hacohen N, Regev A, Feng G, Sharp PA, Zhang F Cell. 2014 Sep 24. pii: S0092-8674(14)01163-5. doi: 10.1016/j.cell.2014.09.014.
Improved vectors and genome-wide libraries for CRISPR screening. Sanjana NE, Shalem O, Zhang F Nat Methods. 2014 Aug;11(8):783-4. doi: 10.1038/nmeth.3047.
Crystal structure of Cas9 in complex with guide RNA and target DNA. Nishimasu H, Ran FA, Hsu PD, Konermann S, Shehata SI, Dohmae N, Ishitani R, Zhang F, Nureki O Cell. 2014 Feb 27;156(5):935-49. doi: 10.1016/j.cell.2014.02.001. Epub 2014 Feb 13.
Genome-scale CRISPR-Cas9 knockout screening in human cells. Shalem O, Sanjana NE, Hartenian E, Shi X, Scott DA, Mikkelsen TS, Heckl D, Ebert BL, Root DE, Doench JG, Zhang F Science. 2014 Jan 3;343(6166):84-7. doi: 10.1126/science.1247005. Epub 2013 Dec 12.
Genome engineering using the CRISPR-Cas9 system. Ran FA, Hsu PD, Wright J, Agarwala V, Scott DA, Zhang F Nat Protoc. 2013 Nov;8(11):2281-308. doi: 10.1038/nprot.2013.143. Epub 2013 Oct 24.
Optical control of mammalian endogenous transcription and epigenetic states. Konermann S, Brigham MD, Trevino A, Hsu PD, Heidenreich M, Le Cong, Platt RJ, Scott DA, Church GM, Zhang F Nature. 2013 Jul 23. doi: 10.1038/nature12466.
Multiplex Genome Engineering Using CRISPR/Cas Systems. Cong L, Ran FA, Cox D, Lin S, Barretto R, Habib N, Hsu PD, Wu X, Jiang W, Marraffini LA, Zhang F Science. 2013 Jan 3.
Comprehensive interrogation of natural TALE DNA-binding modules and transcriptional repressor domains. Cong L, Zhou R, Kuo YC, Cunniff M, Zhang F Nat Commun. 2012 Jul 24;3:968. doi: 10.1038/ncomms1962.
A transcription activator-like effector toolbox for genome engineering. Sanjana NE, Cong L, Zhou Y, Cunniff MM, Feng G, Zhang F Nat Protoc. 2012 Jan 5;7(1):171-92. doi: 10.1038/nprot.2011.431.
Efficient construction of sequence-specific TAL effectors for modulating mammalian transcription. Zhang F, Cong L, Lodato S, Kosuri S, Church GM, Arlotta P Nat Biotechnol. 2011 Jan 19. ():.
lenti sgRNA(MS2)_puro optimized backbone (unpublished)
Feng Zhang lab bacterial Cas9 orthologs (unpublished)
TALE Nuclease (TALEN) (unpublished)
ID Plasmid Gene/Insert Vector Type Publication Hidden Extra Search Info  
27961pLenti-EF1a-Backbone(NN)TAL-Backbone(NN) (Synthetic)Mammalian Expression, LentiviralEfficient construction of sequence-specific TAL effectors for modulating mammalian transcription. Nat Biotechnol. 2011 Jan 19. ():. pLenti Add to Cart
27962pLenti-EF1a-Backbone(NI)TAL-Backbone(NI) (Synthetic)Mammalian Expression, LentiviralEfficient construction of sequence-specific TAL effectors for modulating mammalian transcription. Nat Biotechnol. 2011 Jan 19. ():. pLenti Add to Cart
27963pLenti-EF1a-Backbone(NG)TAL-Backbone(NG) (Synthetic)Mammalian Expression, LentiviralEfficient construction of sequence-specific TAL effectors for modulating mammalian transcription. Nat Biotechnol. 2011 Jan 19. ():. pLenti Add to Cart
27964pLenti-EF1a-Backbone(HD)TAL-Backbone(HD) (Synthetic)Mammalian Expression, LentiviralEfficient construction of sequence-specific TAL effectors for modulating mammalian transcription. Nat Biotechnol. 2011 Jan 19. ():. pLenti Add to Cart
27965monomer-NNTAL-monomer-NNBacterial ExpressionEfficient construction of sequence-specific TAL effectors for modulating mammalian transcription. Nat Biotechnol. 2011 Jan 19. ():. pJ201 Add to Cart
27966monomer-NITAL-monomer-NIBacterial ExpressionEfficient construction of sequence-specific TAL effectors for modulating mammalian transcription. Nat Biotechnol. 2011 Jan 19. ():. pJ201 Add to Cart
27967monomer-NGTAL-monomer-NGBacterial ExpressionEfficient construction of sequence-specific TAL effectors for modulating mammalian transcription. Nat Biotechnol. 2011 Jan 19. ():. pJ201 Add to Cart
27968monomer-HDTAL-monomer-HDBacterial ExpressionEfficient construction of sequence-specific TAL effectors for modulating mammalian transcription. Nat Biotechnol. 2011 Jan 19. ():. pJ201 Add to Cart
27969pLenti-EF1a-TALE1TALE1 (Synthetic)Mammalian Expression, LentiviralEfficient construction of sequence-specific TAL effectors for modulating mammalian transcription. Nat Biotechnol. 2011 Jan 19. ():. pLenti Add to Cart
27970pAAV-minCMV-mCherrymCherry (Synthetic)Mammalian Expression, AAVEfficient construction of sequence-specific TAL effectors for modulating mammalian transcription. Nat Biotechnol. 2011 Jan 19. ():. pAAV Add to Cart
31178Monomer NKMonomer-NKBacterial Expression TALE Nuclease (TALEN) (unpublished) pJ201 Add to Cart
31179TALEN BackboneTALE Nuclease BackboneMammalian Expression TALE Nuclease (TALEN) (unpublished) pCMV Add to Cart
32180pNI_v2TALE Monomer NIBacterial ExpressionA transcription activator-like effector toolbox for genome engineering. Nat Protoc. 2012 Jan 5;7(1):171-92. doi: 10.1038/nprot.2011.431. pJ201 Add to Cart
32181pNG_v2TALE Monomer NGBacterial ExpressionA transcription activator-like effector toolbox for genome engineering. Nat Protoc. 2012 Jan 5;7(1):171-92. doi: 10.1038/nprot.2011.431. pJ201 Add to Cart
32182pNN_v2TALE Monomer NNBacterial ExpressionA transcription activator-like effector toolbox for genome engineering. Nat Protoc. 2012 Jan 5;7(1):171-92. doi: 10.1038/nprot.2011.431. pJ201 Add to Cart
32183pHD_v2TALE Monomer HDBacterial ExpressionA transcription activator-like effector toolbox for genome engineering. Nat Protoc. 2012 Jan 5;7(1):171-92. doi: 10.1038/nprot.2011.431. pJ201 Add to Cart
32185pTALETF_v2 (NN)TALE Transcriptional Activator Backbone, NN for 0.5 repeatMammalian ExpressionA transcription activator-like effector toolbox for genome engineering. Nat Protoc. 2012 Jan 5;7(1):171-92. doi: 10.1038/nprot.2011.431. pCMV Add to Cart
32186pTALETF_v2 (NG)TALE Transcriptional Activator Backbone, NG for 0.5 repeatMammalian ExpressionA transcription activator-like effector toolbox for genome engineering. Nat Protoc. 2012 Jan 5;7(1):171-92. doi: 10.1038/nprot.2011.431. pCMV Add to Cart
32187pTALETF_v2 (NI)TALE Transcriptional Activator Backbone, NI for 0.5 repeatMammalian ExpressionA transcription activator-like effector toolbox for genome engineering. Nat Protoc. 2012 Jan 5;7(1):171-92. doi: 10.1038/nprot.2011.431. pCMV Add to Cart
32188pTALETF_v2 (HD)TALE Transcriptional Activator Backbone, HD for 0.5 repeatMammalian ExpressionA transcription activator-like effector toolbox for genome engineering. Nat Protoc. 2012 Jan 5;7(1):171-92. doi: 10.1038/nprot.2011.431. pCMV Add to Cart
32189pTALEN_v2 (NI)TALE Nuclease, NI for 0.5 repeatMammalian ExpressionA transcription activator-like effector toolbox for genome engineering. Nat Protoc. 2012 Jan 5;7(1):171-92. doi: 10.1038/nprot.2011.431. pCMV Add to Cart
32190pTALEN_v2 (NG)TALE Nuclease, NG for 0.5 repeatMammalian ExpressionA transcription activator-like effector toolbox for genome engineering. Nat Protoc. 2012 Jan 5;7(1):171-92. doi: 10.1038/nprot.2011.431. pCMV Add to Cart
32191pTALEN_v2 (NN)TALE Nuclease, NN for 0.5 repeatMammalian ExpressionA transcription activator-like effector toolbox for genome engineering. Nat Protoc. 2012 Jan 5;7(1):171-92. doi: 10.1038/nprot.2011.431. pCMV Add to Cart
32192pTALEN_v2 (HD)TALE Nuclease, HD for 0.5 repeatMammalian ExpressionA transcription activator-like effector toolbox for genome engineering. Nat Protoc. 2012 Jan 5;7(1):171-92. doi: 10.1038/nprot.2011.431. pCMV Add to Cart
35388pLenti-EF1a-SOX2TALE-Sox2 (Synthetic)Mammalian Expression, LentiviralEfficient construction of sequence-specific TAL effectors for modulating mammalian transcription. Nat Biotechnol. 2011 Jan 19. ():. pLenti Add to Cart
35389pLenti-EF1a-KLF4TALE-Klf4 (Synthetic)Mammalian Expression, LentiviralEfficient construction of sequence-specific TAL effectors for modulating mammalian transcription. Nat Biotechnol. 2011 Jan 19. ():. pLenti Add to Cart
35391pLenti-EF1a-OCT4TALE-Oct4 (Synthetic)Mammalian Expression, LentiviralEfficient construction of sequence-specific TAL effectors for modulating mammalian transcription. Nat Biotechnol. 2011 Jan 19. ():. pLenti Add to Cart
35420TALE5TALE5 (Synthetic)Mammalian Expression, LentiviralEfficient construction of sequence-specific TAL effectors for modulating mammalian transcription. Nat Biotechnol. 2011 Jan 19. ():. pLenti
  • Tag / Fusion Protein
    • EGFP (C terminal on insert)
  • Add to Cart
    35421TALE6TALE6 (Synthetic)Mammalian Expression, LentiviralEfficient construction of sequence-specific TAL effectors for modulating mammalian transcription. Nat Biotechnol. 2011 Jan 19. ():. pLenti
  • Tag / Fusion Protein
    • EGFP (C terminal on insert)
  • Add to Cart
    35423TALE8TALE8 (Synthetic)Mammalian Expression, LentiviralEfficient construction of sequence-specific TAL effectors for modulating mammalian transcription. Nat Biotechnol. 2011 Jan 19. ():. pLenti
  • Tag / Fusion Protein
    • EGFP (C terminal on insert)
  • Add to Cart
    35425TALE10TALE10 (Synthetic)Mammalian Expression, LentiviralEfficient construction of sequence-specific TAL effectors for modulating mammalian transcription. Nat Biotechnol. 2011 Jan 19. ():. pLenti
  • Tag / Fusion Protein
    • EGFP (C terminal on insert)
  • Add to Cart
    35426TALE11TALE11 (Synthetic)Mammalian Expression, LentiviralEfficient construction of sequence-specific TAL effectors for modulating mammalian transcription. Nat Biotechnol. 2011 Jan 19. ():. pLenti Add to Cart
    35427TALE12TALE12 (Synthetic)Mammalian Expression, LentiviralEfficient construction of sequence-specific TAL effectors for modulating mammalian transcription. Nat Biotechnol. 2011 Jan 19. ():. pLenti
  • Tag / Fusion Protein
    • EGFP (C terminal on insert)
  • Add to Cart
    35431hAAVS1 1L TALENhAAVS1 1L TALENMammalian ExpressionA transcription activator-like effector toolbox for genome engineering. Nat Protoc. 2012 Jan 5;7(1):171-92. doi: 10.1038/nprot.2011.431. TALEN designed to target the human AAVS1 locus TALEN BB Add to Cart
    35432hAAVS1 1R TALENhAAVS1 1R TALENMammalian ExpressionA transcription activator-like effector toolbox for genome engineering. Nat Protoc. 2012 Jan 5;7(1):171-92. doi: 10.1038/nprot.2011.431. TALEN designed to target the human AAVS1 locus TALEN BB Add to Cart
    37527Monomer NH_V2Monomer NH (Synthetic)Comprehensive interrogation of natural TALE DNA-binding modules and transcriptional repressor domains. Nat Commun. 2012 Jul 24;3:968. doi: 10.1038/ncomms1962. template for PCR amplification of the monomer used for assembly of the DNA-binding tandem repeat region of the TALE. PJ201 Add to Cart
    37529Monomer NA_V2Monomer NA (Synthetic)Comprehensive interrogation of natural TALE DNA-binding modules and transcriptional repressor domains. Nat Commun. 2012 Jul 24;3:968. doi: 10.1038/ncomms1962. PJ201 Add to Cart
    41863pX097-U6-SpTracrRNA-EF1a-hSpRNaseIII-mCherryU6-SpTracrRNA-EF1a-hSpRNaseIII-mCherryMammalian Expression, Lentiviral, CRISPRMultiplex Genome Engineering Using CRISPR/Cas Systems. Science. 2013 Jan 3. This plasmid is used to reconstitute the complete Type II CRISPR system from S. pyogenes and contains the host factor RNaseIII and tracrRNA. pLenti Add to Cart
    42229pX260-U6-DR-BB-DR-Cbh-NLS-hSpCas9-NLS-H1-shorttracr-PGK-purohumanized S. pyogenes Cas9Mammalian Expression, CRISPRMultiplex Genome Engineering Using CRISPR/Cas Systems. Science. 2013 Jan 3. This plasmid separately encodes a human codon-optimized SpCas9, a tracrRNA and customizable crRNA. pUC ori vector Add to Cart
    42230pX330-U6-Chimeric_BB-CBh-hSpCas9humanized S. pyogenes Cas9Mammalian Expression, CRISPRMultiplex Genome Engineering Using CRISPR/Cas Systems. Science. 2013 Jan 3. A human codon-optimized SpCas9 and chimeric guide RNA expression plasmid. pUC ori vector
  • Tag / Fusion Protein
    • 3xFLAG (N terminal on insert)
  • Add to Cart
    42333pX334-U6-DR-BB-DR-Cbh-NLS-hSpCas9n(D10A)-NLS-H1-shorttracr-PGK-purohumanized S. pyogenes Cas9 (D10A) nickaseMammalian Expression, CRISPRMultiplex Genome Engineering Using CRISPR/Cas Systems. Science. 2013 Jan 3. This plasmid separately encodes a human codon-optimized SpCas9 nickase, a tracrRNA and customizable crRNA. pUC ori vector
  • Tag / Fusion Protein
    • HA (N terminal on insert)
  • Add to Cart
    42335pX335-U6-Chimeric_BB-CBh-hSpCas9n(D10A)humanized S. pyogenes Cas9 (D10A) nickaseMammalian Expression, CRISPRMultiplex Genome Engineering Using CRISPR/Cas Systems. Science. 2013 Jan 3. A human codon-optimized SpCas9 nickase and chimeric guide RNA expression plasmid. pUC ori vector
  • Tag / Fusion Protein
    • HA (N terminal on insert)
  • Add to Cart
    42337pX261-U6-DR-hEmx1-DR-Cbh-NLS-hSpCas9-NLS-H1-shorttracr-PGK-purohumanized S. pyogenes Cas9Mammalian Expression, CRISPRMultiplex Genome Engineering Using CRISPR/Cas Systems. Science. 2013 Jan 3. Dual expression plasmid of human codon-optimized SpCas9 and a gRNA to the human Emx1 locus, can be used to test SpCas9 cleavage in cell lines of choice. pUC ori vector
  • Tag / Fusion Protein
    • 3xFLAG (N terminal on insert)
  • Add to Cart
    42581pAAV_TALE-TF(VP64)-BB_V3TALE-TF(VP64)-BBMammalian Expression, AAV ; Adeno-associated Virus ()Comprehensive interrogation of natural TALE DNA-binding modules and transcriptional repressor domains. Nat Commun. 2012 Jul 24;3:968. doi: 10.1038/ncomms1962. pAAV Add to Cart
    42588pAAV_TALEN-FokI(WT)-BB_V3TALEN-FokI(WT)-BBMammalian Expression, AAV ; Adeno-associated Virus ()Comprehensive interrogation of natural TALE DNA-binding modules and transcriptional repressor domains. Nat Commun. 2012 Jul 24;3:968. doi: 10.1038/ncomms1962. pAAV
  • Tag / Fusion Protein
    • 3XFlag (N terminal on backbone)
  • Add to Cart
    42945SOX2 TALE Repressor (KRAB 1-75)SOX2 TALE Repressor (KRAB 1-75)Mammalian Expression, LentiviralComprehensive interrogation of natural TALE DNA-binding modules and transcriptional repressor domains. Nat Commun. 2012 Jul 24;3:968. doi: 10.1038/ncomms1962. pLenti
  • Tag / Fusion Protein
    • 2A-GFP (C terminal on insert)
  • Add to Cart
    42946SOX2 TALE Repressor (KRAB 11-75)SOX2 TALE Repressor (KRAB 11-75)Mammalian Expression, LentiviralComprehensive interrogation of natural TALE DNA-binding modules and transcriptional repressor domains. Nat Commun. 2012 Jul 24;3:968. doi: 10.1038/ncomms1962. pLenti
  • Tag / Fusion Protein
    • 2A-GFP (C terminal on insert)
  • Add to Cart
    43882Enhanced Repressor Domain for TAL Effector (SID4X)SID4X DomainComprehensive interrogation of natural TALE DNA-binding modules and transcriptional repressor domains. Nat Commun. 2012 Jul 24;3:968. doi: 10.1038/ncomms1962. pUC57 Add to Cart
    47445pAAV_hSyn_TALEBB(NN)-NLS-VP64_2A_GFP_WPRE_bGHpATALE BB(N136_0.5NN_C63)-VP64 (Synthetic)Mammalian Expression, AAV ; TALEOptical control of mammalian endogenous transcription and epigenetic states. Nature. 2013 Jul 23. doi: 10.1038/nature12466. AAV TALE synthesis backbone with NN half repeat; fused to nuclear localization sequence linker and VP64 transcriptional activator effector domain. Synapsin promoter for neuronal expression. pAAV_hSyn_WPRE_bGHpA Add to Cart
    47446pAAV_hSyn_TALEBB(HD)-NLS-VP64_2A_GFP_WPRE_bGHpATALE BB(N136_0.5HD_C63)-VP64 (Synthetic)Mammalian Expression, AAV ; TALEOptical control of mammalian endogenous transcription and epigenetic states. Nature. 2013 Jul 23. doi: 10.1038/nature12466. AAV TALE synthesis backbone with HD half repeat; fused to nuclear localization sequence linker and VP64 transcriptional activator effector domain. Synapsin promoter for neuronal expression. pAAV_hSyn_WPRE_bGHpA Add to Cart
    47447pAAV_hSyn_TALEBB(NG)-NLS-VP64_2A_GFP_WPRE_bGHpATALE BB(N136_0.5NG_C63)-VP64 (Synthetic)Mammalian Expression, AAV ; TALEOptical control of mammalian endogenous transcription and epigenetic states. Nature. 2013 Jul 23. doi: 10.1038/nature12466. AAV TALE synthesis backbone with NG half repeat; fused to nuclear localization sequence linker and VP64 transcriptional activator effector domain. Synapsin promoter for neuronal expression. pAAV_hSyn_WPRE_bGHpA Add to Cart
    47448pAAV_hSyn_TALEBB(NI)-NLS-VP64_2A_GFP_WPRE_bGHpATALE BB(N136_0.5NI_C63)-VP64 (Synthetic)Mammalian Expression, AAV ; TALEOptical control of mammalian endogenous transcription and epigenetic states. Nature. 2013 Jul 23. doi: 10.1038/nature12466. AAV TALE synthesis backbone with NI half repeat; fused to nuclear localization sequence linker and VP64 transcriptional activator effector domain. Synapsin promoter for neuronal expression. pAAV_hSyn_WPRE_bGHpA Add to Cart
    47449pAAV_hSyn_TALEBB(NN)-NLS-SID4X_2A_phiLOV2.1_WPRE_bGHpATALE BB(N136_0.5NN_C63)-SID4X (Synthetic)Mammalian Expression, AAV ; TALEOptical control of mammalian endogenous transcription and epigenetic states. Nature. 2013 Jul 23. doi: 10.1038/nature12466. AAV TALE synthesis backbone with NN half repeat; fused to nuclear localization sequence linker and SID4X transcriptional repressor effector domain. Synapsin promoter for neuronal expression. pAAV_hSyn_WPRE_bGHpA Add to Cart
    47450pAAV_hSyn_TALEBB(HD)-NLS-SID4X_2A_phiLOV2.1_WPRE_bGHpATALE BB(N136_0.5HD_C63)-SID4X (Synthetic)Mammalian Expression, AAV ; TALEOptical control of mammalian endogenous transcription and epigenetic states. Nature. 2013 Jul 23. doi: 10.1038/nature12466. AAV TALE synthesis backbone with HD half repeat; fused to nuclear localization sequence linker and SID4X transcriptional repressor effector domain. Synapsin promoter for neuronal expression. pAAV_hSyn_WPRE_bGHpA Add to Cart
    47451pAAV_hSyn_TALEBB(NG)-NLS-SID4X_2A_phiLOV2.1_WPRE_bGHpATALE BB(N136_0.5NG_C63)-SID4X (Synthetic)Mammalian Expression, AAV ; TALEOptical control of mammalian endogenous transcription and epigenetic states. Nature. 2013 Jul 23. doi: 10.1038/nature12466. AAV TALE synthesis backbone with NG half repeat; fused to nuclear localization sequence linker and SID4X transcriptional repressor effector domain. Synapsin promoter for neuronal expression. pAAV_hSyn_WPRE_bGHpA Add to Cart
    47452pAAV_hSyn_TALEBB(NI)-NLS-SID4X_2A_phiLOV2.1_WPRE_bGHpATALE BB(N136_0.5NI_C63)-SID4X (Synthetic)Mammalian Expression, AAV ; TALEOptical control of mammalian endogenous transcription and epigenetic states. Nature. 2013 Jul 23. doi: 10.1038/nature12466. AAV TALE synthesis backbone with NI half repeat; fused to nuclear localization sequence linker and SID4X transcriptional repressor effector domain. Synapsin promoter for neuronal expression. pAAV_hSyn_WPRE_bGHpA Add to Cart
    47453LITE1.0_pAAV_hSyn_TALE(Grm2)-NLS-CIB1_2A_GFP_WPRE_bGHpATALE (N136,Grm2,C63)-cib1 (Synthetic)Mammalian Expression, AAV ; TALEOptical control of mammalian endogenous transcription and epigenetic states. Nature. 2013 Jul 23. doi: 10.1038/nature12466. epiLITE / LITE1.0 TALE-CIB1. CIB1 binds to blue-light-activated CRY2PHR. Particular TALE targeted to Grm2 promoter. Synapsin promoter for neuronal expression. pAAV_hSyn_WPRE_bGHpA Add to Cart
    47454LITE1.0_pAAV_hSyn_CRY2PHR-NLS-SID4X_2A_phiLOV2.1_WPRE_bGHpACRY2PHR-NLS-SID4X (Synthetic)Mammalian Expression, AAVOptical control of mammalian endogenous transcription and epigenetic states. Nature. 2013 Jul 23. doi: 10.1038/nature12466. epiLITE / LITE1.0 CRY2PHR fused to SID4X transcriptional repressor effector domain. Binds to CIB1 upon blue light stimulation. Synapsin promoter for neuronal expression. pAAV_hSyn_WPRE_bGHpA Add to Cart
    47455LITE2.0 pAAV_hSyn_NLS(alpha-imp)-CRY2PHR-NLS-VP64_2A_GFP_WPRE_bGHpANLS(alpha-imp)-CRY2PHR-NLS-VP64 (Synthetic)Mammalian Expression, AAVOptical control of mammalian endogenous transcription and epigenetic states. Nature. 2013 Jul 23. doi: 10.1038/nature12466. LITE2.0 CRY2PHR-VP64. Changes from LITE1.0: N-terminal alpha-importin nuclear localization sequence. Synapsin promoter for neuronal expression. pAAV_hSyn_WPRE_bGHpA Add to Cart
    47456LITE2.0_pAAV_hSyn_TALE(Grm2)-GS-CIB1(NLS*,∆318-334)_WPRE_bGHpATALE(Grm2)-GS-CIB1(NLS*,∆318-334) (Synthetic)Mammalian Expression, AAV ; TALEOptical control of mammalian endogenous transcription and epigenetic states. Nature. 2013 Jul 23. doi: 10.1038/nature12466. LITE2.0 TALE-CIB1. Changes from LITE1.0: glycine-serine linker, mutated endogenous NLS, aa318-334 deletion. This particular TALE targeted to Grm2. Synapsin promoter for neuronal expression. pAAV_hSyn_WPRE_bGHpA Add to Cart
    47457LITE2.0 EF1a_NLS(alpha-imp)-CRY2PHR-NLS-VP64_2A_GFP_WPRE_hGHpANLS(alpha-imp)-CRY2PHR-NLS-VP64 (Synthetic)Mammalian ExpressionOptical control of mammalian endogenous transcription and epigenetic states. Nature. 2013 Jul 23. doi: 10.1038/nature12466. LITE2.0 CRY2PHR-VP64. Changes from LITE1.0: N-terminal alpha-importin nuclear localization sequence. EF1-alpha promoter for ubiquitous mammalian expression. pAAV_EF1a_WPRE_hGHpA Add to Cart
    47458LITE2.0 EF1a_TALE(Neurog2)-GS-CIB1(NLS*,∆318-334)_WPRE_hGHpATALE (Neurog2)-GS-CIB1(NLS*,∆318-334) (Synthetic)Mammalian ExpressionOptical control of mammalian endogenous transcription and epigenetic states. Nature. 2013 Jul 23. doi: 10.1038/nature12466. LITE2.0 TALE-CIB1. Changes from LITE1.0: glycine-serine linker, mutated endogenous NLS, aa318-334 deletion. This particular TALE targeted to Neurog2. EF1-alpha promoter for ubiquitous mammalian exp. pAAV_EF1a_WPRE_hGHpA Add to Cart
    48137pSpCas9 (PX165)hSpCas9 (Synthetic)Mammalian Expression, CRISPRGenome engineering using the CRISPR-Cas9 system. Nat Protoc. 2013 Nov;8(11):2281-308. doi: 10.1038/nprot.2013.143. Epub 2013 Oct 24. Human codon optimized Cas9 nuclease from Streptococcus pyogenes (Cbh-3X-FLAG-NLS-SpCas9-NLS) PX165 Add to Cart
    48138pSpCas9(BB)-2A-GFP (PX458)hSpCas9 (Synthetic)Mammalian Expression, CRISPRGenome engineering using the CRISPR-Cas9 system. Nat Protoc. 2013 Nov;8(11):2281-308. doi: 10.1038/nprot.2013.143. Epub 2013 Oct 24. Cas9 from S. pyogenes with 2A-EGFP, and cloning backbone for sgRNA PX458 Add to Cart
    48139pSpCas9(BB)-2A-Puro (PX459)hSpCas9-2A-Puro (Synthetic)Mammalian Expression, CRISPRGenome engineering using the CRISPR-Cas9 system. Nat Protoc. 2013 Nov;8(11):2281-308. doi: 10.1038/nprot.2013.143. Epub 2013 Oct 24. NOTE: A new version of this plasmid is now available. See Addgene plasmid 62988. PX459 Add to Cart
    48140pSpCas9n(BB)-2A-GFP (PX461)hSpCas9n (Synthetic)Mammalian Expression, CRISPRGenome engineering using the CRISPR-Cas9 system. Nat Protoc. 2013 Nov;8(11):2281-308. doi: 10.1038/nprot.2013.143. Epub 2013 Oct 24. Cas9n (D10A nickase mutant) from S. pyogenes with 2A-EGFP, and cloning backbone for sgRNA PX461 Add to Cart
    48141pSpCas9n(BB)-2A-Puro (PX462)hSpCas9n-2A-Puro (Synthetic)Mammalian Expression, CRISPRGenome engineering using the CRISPR-Cas9 system. Nat Protoc. 2013 Nov;8(11):2281-308. doi: 10.1038/nprot.2013.143. Epub 2013 Oct 24. NOTE: A new version of this plasmid is now available. See Addgene plasmid 62987 PX462 Add to Cart
    48253LITE1.0_pAAV_hSyn_CRY2PHR-NLS-VP64_2A_GFP_WPRE_bGHpACRY2PHR-NLS-VP64 (Synthetic)Mammalian Expression, AAV ; TALEOptical control of mammalian endogenous transcription and epigenetic states. Nature. 2013 Jul 23. doi: 10.1038/nature12466. LITE1.0 CRY2PHR fused to VP64 transcriptional activator domain. Binds to CIB1 upon blue light stimulation. Synapsin promoter for neuronal expression. See LITE2.0 for optimized LITE activators. pAAV_hSyn_WPRE_bGHpA Add to Cart
    48873pSpCas9n(BB) (PX460)hSpCas9n (Synthetic)Mammalian Expression, CRISPRGenome engineering using the CRISPR-Cas9 system. Nat Protoc. 2013 Nov;8(11):2281-308. doi: 10.1038/nprot.2013.143. Epub 2013 Oct 24. Cas9n (D10A nickase mutant) from S. pyogenes PX460 Add to Cart
    51760lentiCRISPR - EGFP sgRNA 1Cas9 (Synthetic), Puromycin resistance (Other), EGFP sgRNA 1 (Synthetic)Mammalian Expression, Lentiviral, CRISPRGenome-scale CRISPR-Cas9 knockout screening in human cells. Science. 2014 Jan 3;343(6166):84-7. doi: 10.1126/science.1247005. Epub 2013 Dec 12. Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an EGFP targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone. lentiCRISPR (pXPR_001), Addgene plasmid #49535 Add to Cart
    51761lentiCRISPR - EGFP sgRNA 2Cas9 (Synthetic), Puromycin resistance (Other), EGFP sgRNA 2 (Synthetic)Mammalian Expression, Lentiviral, CRISPRGenome-scale CRISPR-Cas9 knockout screening in human cells. Science. 2014 Jan 3;343(6166):84-7. doi: 10.1126/science.1247005. Epub 2013 Dec 12. Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an EGFP targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone. lentiCRISPR (pXPR_001), Addgene plasmid #49535 Add to Cart
    51762lentiCRISPR - EGFP sgRNA 3Cas9 (Synthetic), Puromycin resistance (Other), EGFP sgRNA 3 (Synthetic)Mammalian Expression, Lentiviral, CRISPRGenome-scale CRISPR-Cas9 knockout screening in human cells. Science. 2014 Jan 3;343(6166):84-7. doi: 10.1126/science.1247005. Epub 2013 Dec 12. Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an EGFP targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone. lentiCRISPR (pXPR_001), Addgene plasmid #49535 Add to Cart
    51763lentiCRISPR - EGFP sgRNA 4Cas9 (Synthetic), Puromycin resistance (Other), EGFP sgRNA 4 (Synthetic)Mammalian Expression, Lentiviral, CRISPRGenome-scale CRISPR-Cas9 knockout screening in human cells. Science. 2014 Jan 3;343(6166):84-7. doi: 10.1126/science.1247005. Epub 2013 Dec 12. Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an EGFP targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone. lentiCRISPR (pXPR_001), Addgene plasmid #49535 Add to Cart
    51764lentiCRISPR - EGFP sgRNA 5Cas9 (Synthetic), Puromycin resistance (Other), EGFP sgRNA 5 (Synthetic)Lentiviral, CRISPRGenome-scale CRISPR-Cas9 knockout screening in human cells. Science. 2014 Jan 3;343(6166):84-7. doi: 10.1126/science.1247005. Epub 2013 Dec 12. Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an EGFP targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone. lentiCRISPR (pXPR_001), Addgene plasmid #49535 Add to Cart
    51765lentiCRISPR - EGFP sgRNA 6Cas9 (Synthetic), Puromycin resistance (Other), EGFP sgRNA 6 (Synthetic)Mammalian Expression, Lentiviral, CRISPRGenome-scale CRISPR-Cas9 knockout screening in human cells. Science. 2014 Jan 3;343(6166):84-7. doi: 10.1126/science.1247005. Epub 2013 Dec 12. Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an EGFP targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone. lentiCRISPR (pXPR_001), Addgene plasmid #49535 Add to Cart
    52961lentiCRISPR v2Cas9 (Synthetic), Puromycin resistanceMammalian Expression, Lentiviral, CRISPRImproved vectors and genome-wide libraries for CRISPR screening. Nat Methods. 2014 Aug;11(8):783-4. doi: 10.1038/nmeth.3047. Replaces original lentiCRISPRv1 (Addgene Plasmid 49535) and produces ~10-fold higher titer virus. 3rd generation lentiviral backbone. Custom Add to Cart
    52962lentiCas9-BlastCas9 (Synthetic), Blasticidin resistanceMammalian Expression, Lentiviral, CRISPRImproved vectors and genome-wide libraries for CRISPR screening. Nat Methods. 2014 Aug;11(8):783-4. doi: 10.1038/nmeth.3047. Expresses human codon-optimized S. pyogenes Cas9 protein and blasticidin resistance from EFS promoter. Lentiviral backbone. pFUGW Add to Cart
    52963lentiGuide-PuroS. pyogenes sgRNA cassette (Synthetic), Puromycin resistanceMammalian Expression, Lentiviral, CRISPRImproved vectors and genome-wide libraries for CRISPR screening. Nat Methods. 2014 Aug;11(8):783-4. doi: 10.1038/nmeth.3047. Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and puromycin resistance from EF-1a promoter. Lentiviral backbone. Custom Add to Cart
    58888PX543 SpCas9(del175-307)hSpCas9 (Synthetic)Mammalian Expression, CRISPRCrystal structure of Cas9 in complex with guide RNA and target DNA. Cell. 2014 Feb 27;156(5):935-49. doi: 10.1016/j.cell.2014.02.001. Epub 2014 Feb 13. Truncated form of SpCas9 without REC2 domain PX165 Add to Cart
    58889PX544 SpCas9(del1098-1368)hSpCas9 (Synthetic)Mammalian Expression, CRISPRCrystal structure of Cas9 in complex with guide RNA and target DNA. Cell. 2014 Feb 27;156(5):935-49. doi: 10.1016/j.cell.2014.02.001. Epub 2014 Feb 13. Truncated form of SpCas9 without C' domain PX165 Add to Cart
    60224AAV:ITR-U6-sgRNA(Kras)-U6-sgRNA(p53)-U6-sgRNA(Lkb1)-pEFS-Rluc-2A-Cre-shortPA-KrasG12D_HDRdonor-ITR (AAV-KPL)sgRNA (Synthetic), Renilla luciferase, Cre recombinase, KrasG12D HDR donorMammalian Expression, Mouse Targeting, AAV, Cre/Lox, CRISPRCRISPR-Cas9 Knockin Mice for Genome Editing and Cancer Modeling. Cell. 2014 Sep 24. pii: S0092-8674(14)01163-5. doi: 10.1016/j.cell.2014.09.014. Expresses Cre recombinase and Renilla luciferase from the EFS promoter and three U6-driven sgRNAs targeting Kras, p53, and Lkb1. Contains KrasG12D HDR donor. AAV backbone. AAV Add to Cart
    60225AAV:ITR-U6-sgRNA(LacZ)-pEFS-Rluc-2A-Cre-WPRE-hGHpA-ITRsgRNA, Renilla luciferase, Cre recombinaseMammalian Expression, Mouse Targeting, AAV, Cre/Lox, CRISPR, LuciferaseCRISPR-Cas9 Knockin Mice for Genome Editing and Cancer Modeling. Cell. 2014 Sep 24. pii: S0092-8674(14)01163-5. doi: 10.1016/j.cell.2014.09.014. Expresses Cre recombinase and Renilla luciferase from the EFS promoter and one U6-driven sgRNA targeting LacZ. AAV backbone. AAV Add to Cart
    60226AAV:ITR-U6-sgRNA(backbone)-pEFS-Rluc-2A-Cre-WPRE-hGHpA-ITRsgRNA, Renilla luciferase, Cre recombinaseMammalian Expression, Mouse Targeting, AAV, Cre/Lox, CRISPR, LuciferaseCRISPR-Cas9 Knockin Mice for Genome Editing and Cancer Modeling. Cell. 2014 Sep 24. pii: S0092-8674(14)01163-5. doi: 10.1016/j.cell.2014.09.014. Expresses Cre recombinase and Renilla luciferase from the EFS promoter and one U6-driven sgRNA. AAV backbone with SapI spacer for sgRNA cloning. AAV Add to Cart
    60227AAV:ITR-U6-sgRNA(NeuN)-pCBh-Cre-WPRE-hGHpA-ITRsgRNA, Cre recombinaseMammalian Expression, Mouse Targeting, AAV, Cre/Lox, CRISPRCRISPR-Cas9 Knockin Mice for Genome Editing and Cancer Modeling. Cell. 2014 Sep 24. pii: S0092-8674(14)01163-5. doi: 10.1016/j.cell.2014.09.014. Expresses Cre recombinase from the Cbh promoter and one U6-driven sgRNA targeting the mouse gene NeuN. AAV backbone. AAV Add to Cart
    60228AAV:ITR-U6-sgRNA(LacZ)-pCBh-Cre-WPRE-hGHpA-ITRsgRNA, Cre recombinaseMammalian Expression, Mouse Targeting, AAV, Cre/Lox, CRISPRCRISPR-Cas9 Knockin Mice for Genome Editing and Cancer Modeling. Cell. 2014 Sep 24. pii: S0092-8674(14)01163-5. doi: 10.1016/j.cell.2014.09.014. Expresses Cre recombinase from the Cbh promoter and one U6-driven sgRNA control targeting LacZ. AAV backbone. AAV Add to Cart
    60229AAV:ITR-U6-sgRNA(backbone)-pCBh-Cre-WPRE-hGHpA-ITRsgRNA, Cre recombinaseMammalian Expression, Mouse Targeting, AAV, Cre/Lox, CRISPRCRISPR-Cas9 Knockin Mice for Genome Editing and Cancer Modeling. Cell. 2014 Sep 24. pii: S0092-8674(14)01163-5. doi: 10.1016/j.cell.2014.09.014. Expresses Cre recombinase from the Cbh promoter and one U6-driven sgRNA. AAV backbone with SapI spacer for sgRNA cloning. AAV Add to Cart
    60230AAV:ITR-U6-sgRNA(NeuN)-hSyn-Cre-2A-EGFP-KASH-WPRE-shortPA-ITRsgRNA, Cre recombinase, EGFPMammalian Expression, Mouse Targeting, AAV, Cre/Lox, CRISPRCRISPR-Cas9 Knockin Mice for Genome Editing and Cancer Modeling. Cell. 2014 Sep 24. pii: S0092-8674(14)01163-5. doi: 10.1016/j.cell.2014.09.014. Expresses Cre recombinase and KASH-tagged EGFP from the hSyn promoter and one U6-driven sgRNA. AAV backbone with SapI spacer for sgRNA cloning. AAV Add to Cart
    60231AAV:ITR-U6-sgRNA(backbone)-hSyn-Cre-2A-EGFP-KASH-WPRE-shortPA-ITRsgRNA, Cre recombinase, EGFPMammalian Expression, Mouse Targeting, AAV, Cre/Lox, CRISPRCRISPR-Cas9 Knockin Mice for Genome Editing and Cancer Modeling. Cell. 2014 Sep 24. pii: S0092-8674(14)01163-5. doi: 10.1016/j.cell.2014.09.014. Expresses Cre recombinase and KASH-tagged EGFP from the hSyn promoter and one U6-driven sgRNA. AAV backbone with SapI spacer for sgRNA cloning. AAV Add to Cart
    60957PX551SpCas9 (Synthetic)Mammalian Expression, AAV, CRISPRIn vivo interrogation of gene function in the mammalian brain using CRISPR-Cas9. Nat Biotechnol. 2014 Oct 19. doi: 10.1038/nbt.3055. pAAV-pMecp2-SpCas9-spA (AAV-SpCas9). AAV plasmid expressing Cas9 in neurons under control of truncated mecp2 promoter. pAAV Add to Cart
    60958PX552U6_(SpaI)_sgRNA (Synthetic), Syn_EGFP-KASH (Synthetic)Mammalian Expression, Mouse Targeting, AAV, CRISPRIn vivo interrogation of gene function in the mammalian brain using CRISPR-Cas9. Nat Biotechnol. 2014 Oct 19. doi: 10.1038/nbt.3055. pAAV-U6sgRNA(SapI)_hSyn-GFP-KASH-bGH (SpGuide acceptor). AAV plasmid for sgRNA cloning. GFP-KASH fusion facilitates FACS sorting of cells and nuclei. pAAV Add to Cart
    61408LSL-Cas9-Rosa26TVCas9 (Synthetic), EGFPMammalian Expression, Mouse Targeting, Cre/Lox, CRISPRCRISPR-Cas9 Knockin Mice for Genome Editing and Cancer Modeling. Cell. 2014 Sep 24. pii: S0092-8674(14)01163-5. doi: 10.1016/j.cell.2014.09.014. Cre-dependent Cas9 expression plasmid and targeting vector for the mouse Rosa26 locus. Cas9 expression is Cre-dependent and the targeting vector has positive (Neo) and negative (DTA) selection. Ai9 Add to Cart
    61422dCAS9-VP64_GFPdCAS9(D10A,H840A)-VP64_2A_GFP (Synthetic)Mammalian Expression, Lentiviral, CRISPRGenome-scale transcriptional activation by an engineered CRISPR-Cas9 complex. Nature. 2014 Dec 10. doi: 10.1038/nature14136. Expresses dCAS9-VP64 activator with 2A GFP lenti(AMP) Add to Cart
    61423MS2-P65-HSF1_GFPMS2(N55K)-P65-HSF1_2A_GFP (Homo sapiens)Mammalian Expression, CRISPRGenome-scale transcriptional activation by an engineered CRISPR-Cas9 complex. Nature. 2014 Dec 10. doi: 10.1038/nature14136. Expresses the MS2-P65-HSF1 activator helper complex with a 2A GFP lenti(AMP) Add to Cart
    61424sgRNA(MS2) cloning backboneMammalian Expression, CRISPRGenome-scale transcriptional activation by an engineered CRISPR-Cas9 complex. Nature. 2014 Dec 10. doi: 10.1038/nature14136. sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2. Contains BbsI sites for insertion of spacer sequences. pUC19 Add to Cart
    61425lenti dCAS-VP64_BlastdCAS9(D10A, N863A)-VP64_2A_Blast (Synthetic)Mammalian Expression, Lentiviral, CRISPRGenome-scale transcriptional activation by an engineered CRISPR-Cas9 complex. Nature. 2014 Dec 10. doi: 10.1038/nature14136. 3rd generation lenti vector encoding dCAS9-VP64 with 2A Blast resistance marker (EF1a-NLS-dCas9(N863)-VP64-2A-Blast-WPRE) plenti Add to Cart
    61426lenti MS2-P65-HSF1_HygroMS2-P65-HSF1_2A_Hygro (Homo sapiens)Mammalian Expression, Lentiviral, CRISPRGenome-scale transcriptional activation by an engineered CRISPR-Cas9 complex. Nature. 2014 Dec 10. doi: 10.1038/nature14136. lenti vector encoding the MS2-P65-HSF1 activator helper complex with a 2A Hygro resistance marker (EF1a-MS2-p65-HSF1-2A-Hygro-WPRE) plenti Add to Cart
    61427lenti sgRNA(MS2)_zeo backboneMammalian Expression, Lentiviral, CRISPRGenome-scale transcriptional activation by an engineered CRISPR-Cas9 complex. Nature. 2014 Dec 10. doi: 10.1038/nature14136. 3rd generation lenti sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2 and EF1a-zeo resistance marker. Contains BsmBI sites for insertion of spacer sequences. plenti Add to Cart
    61591pX601-AAV-CMV::NLS-SaCas9-NLS-3xHA-bGHpA;U6::BsaI-sgRNAhSaCas9 (Other), Chimeric guide for SaCas9 (Other)Mammalian Expression, AAV, CRISPRIn vivo genome editing using Staphylococcus aureus Cas9. Nature. 2015 Apr 1. doi: 10.1038/nature14299. A single vector AAV-Cas9 system containing Cas9 from Staphylococcus aureus (SaCas9) and its sgRNA. pAAV Add to Cart
    61592pX600-AAV-CMV::NLS-SaCas9-NLS-3xHA-bGHpAhSaCas9 (Other)Mammalian Expression, AAV, CRISPRIn vivo genome editing using Staphylococcus aureus Cas9. Nature. 2015 Apr 1. doi: 10.1038/nature14299. A human codon-optimized Cas9 from Staphylococcus aureus (SaCas9). pAAV Add to Cart
    61593pX602-AAV-TBG::NLS-SaCas9-NLS-HA-OLLAS-bGHpA;U6::BsaI-sgRNAhSaCas9 (Other), Chimeric guide for SaCas9 (Other)Mammalian Expression, AAV, CRISPRIn vivo genome editing using Staphylococcus aureus Cas9. Nature. 2015 Apr 1. doi: 10.1038/nature14299. A single vector AAV-Cas9 system containing Cas9 from Staphylococcus aureus (SaCas9) and its sgRNA. pAAV Add to Cart
    61594pX603-AAV-CMV::NLS-dSaCas9(D10A,N580A)-NLS-3xHA-bGHpAhSaCas9 (Other)Mammalian Expression, AAV, CRISPRIn vivo genome editing using Staphylococcus aureus Cas9. Nature. 2015 Apr 1. doi: 10.1038/nature14299. A catalytically inactive SaCas9 (dSaCas9) with the D10A and N580A mutations. pAAV Add to Cart
    61597Human SAM libraryHuman SAM library (Synthetic)Mammalian Expression, Lentiviral, CRISPRGenome-scale transcriptional activation by an engineered CRISPR-Cas9 complex. Nature. 2014 Dec 10. doi: 10.1038/nature14136. SAM can be combined with a human genome-wide library to activate all known coding isoforms from the RefSeq database (23,430 isoforms). lenti sgRNA(MS2)_zeo backbone (Addgene plasmid 61427) Add to Cart
    62883PX851SpCas9 (aa 2-535)Mammalian Expression, CRISPRA split-Cas9 architecture for inducible genome editing and transcription modulation. Nat Biotechnol. 2015 Feb 2. doi: 10.1038/nbt.3149. N-term SpCas9 piece of inducible split-4 (Cas9(N)-FRB-NES split-4). PX330 Add to Cart
    62884PX852SpCas9 (aa536-1368)Mammalian Expression, CRISPRA split-Cas9 architecture for inducible genome editing and transcription modulation. Nat Biotechnol. 2015 Feb 2. doi: 10.1038/nbt.3149. C-term SpCas9 piece of inducible split-4 (Cas9(C)-FKBP split-4). PX330 Add to Cart
    62885PX853SpCas9 (aa 2-573)Mammalian Expression, CRISPRA split-Cas9 architecture for inducible genome editing and transcription modulation. Nat Biotechnol. 2015 Feb 2. doi: 10.1038/nbt.3149. N-term SpCas9 piece of inducible split-5 (Cas9(N)-FRB-NES split-5). PX330 Add to Cart
    62886PX854SpCas9 (aa573-1368)Mammalian Expression, CRISPRA split-Cas9 architecture for inducible genome editing and transcription modulation. Nat Biotechnol. 2015 Feb 2. doi: 10.1038/nbt.3149. C-term SpCas9 piece of inducible split-5 (Cas9(C)-FKBP split-5). PX330 Add to Cart
    62887PX855SpCas9 (aa 2-535)Mammalian Expression, CRISPRA split-Cas9 architecture for inducible genome editing and transcription modulation. Nat Biotechnol. 2015 Feb 2. doi: 10.1038/nbt.3149. N-term SpCas9 piece of inducible transcriptional activator (dCas9(N)-FRB-2xNES) PX330 Add to Cart
    62888PX856SpCas9 (aa536-1368)Mammalian Expression, CRISPRA split-Cas9 architecture for inducible genome editing and transcription modulation. Nat Biotechnol. 2015 Feb 2. doi: 10.1038/nbt.3149. C-term SpCas9 piece of inducible transcriptional activator (dCas9(C)-FKBP-2xNLS-VP64) PX330 Add to Cart
    62889pLSC-5SpCas9(574-1368), SpCas9(2-573)Mammalian Expression, Lentiviral, CRISPRA split-Cas9 architecture for inducible genome editing and transcription modulation. Nat Biotechnol. 2015 Feb 2. doi: 10.1038/nbt.3149. Lenti vector for expression of inducible split Cas9 lentiCRISPR Add to Cart
    62987pSpCas9n(BB)-2A-Puro (PX462) V2.0hSpCas9n-2A-Puro (Synthetic)Mammalian Expression, CRISPRGenome engineering using the CRISPR-Cas9 system. Nat Protoc. 2013 Nov;8(11):2281-308. doi: 10.1038/nprot.2013.143. Epub 2013 Oct 24. Cas9n (D10A nickase mutant) from S. pyogenes with 2A-Puro, and cloning backbone for sgRNA (V2.0) PX462 Add to Cart
    62988pSpCas9(BB)-2A-Puro (PX459) V2.0hSpCas9-2A-Puro V2.0 (Synthetic)Mammalian Expression, CRISPRGenome engineering using the CRISPR-Cas9 system. Nat Protoc. 2013 Nov;8(11):2281-308. doi: 10.1038/nprot.2013.143. Epub 2013 Oct 24. Cas9 from S. pyogenes with 2A-Puro, and cloning backbone for sgRNA (V2.0) PX459 Add to Cart
    63592lentiCas9-EGFPCas9 (Synthetic), EGFP (Other)Mammalian Expression, Lentiviral, CRISPRGenome-wide CRISPR screen in a mouse model of tumor growth and metastasis. Cell. 2015 Mar 12;160(6):1246-60. doi: 10.1016/j.cell.2015.02.038. Epub 2015 Mar 5. Expresses human codon-optimized S. pyogenes Cas9 protein and EGFP from EFS promoter. Lentiviral backbone. pFUGW Add to Cart
    63593lentiCas9n(D10A)-BlastCas9 (Synthetic), Blasticidin resistanceMammalian Expression, Lentiviral, CRISPRImproved vectors and genome-wide libraries for CRISPR screening. Nat Methods. 2014 Aug;11(8):783-4. doi: 10.1038/nmeth.3047. Expresses human codon-optimized S. pyogenes Cas9n (D10A nickase) protein and blasticidin resistance from EFS promoter. Lentiviral backbone. pFUGW Add to Cart
    68314PX377 Corynebacter diphtheriae Cas9CdCas9 (Synthetic)Mammalian Expression, CRISPR Feng Zhang lab bacterial Cas9 orthologs (unpublished) Corynebacter diphtheriae Cas9 pDREAM Add to Cart
    68315PX378 Sutterella wadsworthensis Cas9SwCas9 (Synthetic)Mammalian Expression, CRISPR Feng Zhang lab bacterial Cas9 orthologs (unpublished) Sutterella wadsworthensis Cas9 pDREAM Add to Cart
    68316PX379 Legionella pneumophila str. Paris Cas9LpCas9 (Synthetic)Mammalian Expression, CRISPR Feng Zhang lab bacterial Cas9 orthologs (unpublished) Legionella pneumophila str. Paris Cas9 pDREAM Add to Cart
    68317PX380 Treponema denticola Cas9TdCas9 (Synthetic)Mammalian Expression, CRISPR Feng Zhang lab bacterial Cas9 orthologs (unpublished) Treponema denticola Cas9 pDREAM Add to Cart
    68318PX381 Filifactor alocis Cas9FaCas9 (Synthetic)Mammalian Expression, CRISPR Feng Zhang lab bacterial Cas9 orthologs (unpublished) Filifactor alocis Cas9 pDREAM Add to Cart
    68319PX382 Staphylococcus pseudintermedius Cas9SpsCas9 (Synthetic)Mammalian Expression, CRISPR Feng Zhang lab bacterial Cas9 orthologs (unpublished) Staphylococcus pseudintermedius Cas9 pDREAM Add to Cart
    68320PX383 Lactobacillus johnsonii Cas9LjCas9 (Synthetic)Mammalian Expression, CRISPR Feng Zhang lab bacterial Cas9 orthologs (unpublished) Lactobacillus johnsonii Cas9 pDREAM Add to Cart
    68322PX385 Streptococcus pasteurianus Cas9SpaCas9 (Synthetic)Mammalian Expression, CRISPR Feng Zhang lab bacterial Cas9 orthologs (unpublished) Streptococcus pasteurianus Cas9 pDREAM Add to Cart
    68323PX386 Lactobacillus farciminis Cas9LfCas9 (Synthetic)Mammalian Expression, CRISPR Feng Zhang lab bacterial Cas9 orthologs (unpublished) Lactobacillus farciminis Cas9 pDREAM Add to Cart
    68324PX387 Mycoplasma mobile Cas9MmCas9 (Synthetic)Mammalian Expression, CRISPR Feng Zhang lab bacterial Cas9 orthologs (unpublished) Mycoplasma mobile Cas9 pDREAM Add to Cart
    68325PX388 Bacteroides coprophilus Cas9BcCas9 (Synthetic)Mammalian Expression, CRISPR Feng Zhang lab bacterial Cas9 orthologs (unpublished) Bacteroides coprophilus Cas9 pDREAM Add to Cart
    68326PX389 Fluviicola taffensis Cas9FtCas9 (Synthetic)Mammalian Expression, CRISPR Feng Zhang lab bacterial Cas9 orthologs (unpublished) Fluviicola taffensis Cas9 pDREAM Add to Cart
    68327PX390 Flavobacterium columnare Cas9FcCas9 (Synthetic)Mammalian Expression, CRISPR Feng Zhang lab bacterial Cas9 orthologs (unpublished) Flavobacterium columnare Cas9 pDREAM Add to Cart
    68328PX391 Sphaerochaeta globus str. Buddy Cas9SgCas9 (Synthetic)Mammalian Expression, CRISPR Feng Zhang lab bacterial Cas9 orthologs (unpublished) Sphaerochaeta globus str. Buddy Cas9 pDREAM Add to Cart
    68329PX392 Azospirillum B510 Cas9AzoCas9 (Synthetic)Mammalian Expression, CRISPR Feng Zhang lab bacterial Cas9 orthologs (unpublished) Azospirillum B510 Cas9 pDREAM Add to Cart
    68330PX393 Gluconacetobacter diazotrophicus Cas9GdCas9 (Synthetic)Mammalian Expression, CRISPR Feng Zhang lab bacterial Cas9 orthologs (unpublished) Gluconacetobacter diazotrophicus Cas9 pDREAM Add to Cart
    68331PX394 Neisseria cinerea Cas9NcCas9 (Synthetic)Mammalian Expression, CRISPR Feng Zhang lab bacterial Cas9 orthologs (unpublished) Neisseria cinerea Cas9 pDREAM Add to Cart
    68332PX395 Roseburia intestinalis Cas9RiCas9 (Synthetic)Mammalian Expression, CRISPR Feng Zhang lab bacterial Cas9 orthologs (unpublished) Roseburia intestinalis Cas9 pDREAM Add to Cart
    68333PX396 Parvibaculum lavamentivorans Cas9PlCas9 (Synthetic)Mammalian Expression, CRISPR Feng Zhang lab bacterial Cas9 orthologs (unpublished) Parvibaculum lavamentivorans Cas9 pDREAM Add to Cart
    68334PX398 Nitratifractor salsuginis str DSM 16511 Cas9NsCas9 (Synthetic)Mammalian Expression, CRISPR Feng Zhang lab bacterial Cas9 orthologs (unpublished) Nitratifractor salsuginis str DSM 16511 Cas9 pDREAM Add to Cart
    68335PX399 Mycoplasma gallisepticum str. F Cas9MgCas9 (Synthetic)Mammalian Expression, CRISPR Feng Zhang lab bacterial Cas9 orthologs (unpublished) Mycoplasma gallisepticum str. F Cas9 pDREAM Add to Cart
    68336PX400 Campylobacter lari CF89-12 Cas9ClCas9 (Synthetic)Mammalian Expression, CRISPR Feng Zhang lab bacterial Cas9 orthologs (unpublished) Campylobacter lari CF89-12 Cas9 pDREAM Add to Cart
    68337PX403 Streptococcus thermophilus LMD-9 CRISPR 3 Cas9St3Cas9 (Synthetic)Mammalian Expression, CRISPR Feng Zhang lab bacterial Cas9 orthologs (unpublished) Streptococcus thermophilus LMD-9 CRISPR 3 Cas9 Unknown Add to Cart
    68338PX404 Campylobacter jejuni Cas9CjCas9 (Synthetic)Mammalian Expression, CRISPR Feng Zhang lab bacterial Cas9 orthologs (unpublished) Campylobacter jejuni Cas9 Unknown Add to Cart
    68702PX405 Neisseria meningitidis Cas9NmCas9 (Synthetic)Mammalian Expression, CRISPR Feng Zhang lab bacterial Cas9 orthologs (unpublished) Neisseria meningitidis Cas9 pDREAM Add to Cart
    68703PX406 Pasteurella multocida Cas9PmCas9 (Synthetic)Mammalian Expression, CRISPR Feng Zhang lab bacterial Cas9 orthologs (unpublished) Pasteurella multocida Cas9 pDREAM Add to Cart
    68705PX408 Francisella tularensis subsp. novicida Cas9FtCas9 (Synthetic)Mammalian Expression, CRISPR Feng Zhang lab bacterial Cas9 orthologs (unpublished) Francisella tularensis subsp. novicida Cas9 pDREAM Add to Cart
    68706PX429 SpCas9 N863A nickaseSpCas9 N863A nicking mutant (Synthetic)Mammalian Expression, CRISPRCrystal structure of Cas9 in complex with guide RNA and target DNA. Cell. 2014 Feb 27;156(5):935-49. doi: 10.1016/j.cell.2014.02.001. Epub 2014 Feb 13. SpCas9 N863A nickase PX165 Add to Cart
    69973pY001 (pFnCpf1_full)FnCpf1 locus (Other)Bacterial Expression, CRISPRCpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas System. Cell. 2015 Sep 23. pii: S0092-8674(15)01200-3. doi: 10.1016/j.cell.2015.09.038. Expresses FnCpf1 locus including Cas genes and spacers 1-4 of CRISPR array. pACYC184 Add to Cart
    69974pY003 (pFnCpf1_delta Cas)FnCpf1 locus without Cas genes (Other)Bacterial Expression, CRISPRCpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas System. Cell. 2015 Sep 23. pii: S0092-8674(15)01200-3. doi: 10.1016/j.cell.2015.09.038. Expresses FnCpf1 and spacers 1-4 of CRISPR array. Cas genes are removed. pACYC184 Add to Cart
    69975pY002 (pFnCpf1_min)FnCpf1 locus without Cas genes nd artificial promoter (Other)Bacterial Expression, CRISPRCpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas System. Cell. 2015 Sep 23. pii: S0092-8674(15)01200-3. doi: 10.1016/j.cell.2015.09.038. Expresses FnCpf1 and spacers 1-4 of CRISPR array under artificial promoter. Cas genes are removed. pACYC184 Add to Cart
    69976pY004 (pcDNA3.1-hFnCpf1)hFnCpf1 (Other)Mammalian Expression, CRISPRCpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas System. Cell. 2015 Sep 23. pii: S0092-8674(15)01200-3. doi: 10.1016/j.cell.2015.09.038. Expresses humanized FnCpf1 pcDNA3.1 Add to Cart
    69977pY005 (pcDNA3.1-hLb3Cpf1)hLb3Cpf1 (Other)Mammalian Expression, CRISPRCpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas System. Cell. 2015 Sep 23. pii: S0092-8674(15)01200-3. doi: 10.1016/j.cell.2015.09.038. Expresses humanized Lb3Cpf1 pcDNA3.1 Add to Cart
    69978pY006 (pcDNA3.1-hBpCpf1)hBpCpf1 (Other)Mammalian Expression, CRISPRCpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas System. Cell. 2015 Sep 23. pii: S0092-8674(15)01200-3. doi: 10.1016/j.cell.2015.09.038. Expresses humanized BpCpf1 pcDNA3.1 Add to Cart
    69979pY007 (pcDNA3.1-hPeCpf1)hPeCpf1 (Other)Mammalian Expression, CRISPRCpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas System. Cell. 2015 Sep 23. pii: S0092-8674(15)01200-3. doi: 10.1016/j.cell.2015.09.038. Expresses humanized PeCpf1 pcDNA3.1 Add to Cart
    69980pY008 (pcDNA3.1-hPbCpf1)hPbCpf1 (Other)Mammalian Expression, CRISPRCpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas System. Cell. 2015 Sep 23. pii: S0092-8674(15)01200-3. doi: 10.1016/j.cell.2015.09.038. Expresses humanized LPbCpf1 pcDNA3.1 Add to Cart
    69981pY009 (pcDNA3.1-hSsCpf1)hSsCpf1 (Other)Mammalian Expression, CRISPRCpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas System. Cell. 2015 Sep 23. pii: S0092-8674(15)01200-3. doi: 10.1016/j.cell.2015.09.038. Expresses humanized SsCpf1 pcDNA3.1 Add to Cart
    69982pY010 (pcDNA3.1-hAsCpf1)hAsCpf1 (Other)Mammalian Expression, CRISPRCpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas System. Cell. 2015 Sep 23. pii: S0092-8674(15)01200-3. doi: 10.1016/j.cell.2015.09.038. Expresses humanized AsCpf1 pcDNA3.1 Add to Cart
    69983pY011 (pcDNA3.1-hLb2Cpf1)hLb2Cpf1 (Other)Mammalian Expression, CRISPRCpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas System. Cell. 2015 Sep 23. pii: S0092-8674(15)01200-3. doi: 10.1016/j.cell.2015.09.038. Expresses humanized Lb2Cpf1 pcDNA3.1 Add to Cart
    69984pY012 (pcDNA3.1-hCMtCpf1)hCMtCpf1 (Other)Mammalian Expression, CRISPRCpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas System. Cell. 2015 Sep 23. pii: S0092-8674(15)01200-3. doi: 10.1016/j.cell.2015.09.038. Expresses humanized CMtCpf1 pcDNA3.1 Add to Cart
    69985pY013 (pcDNA3.1-hEeCpf1)hEeCpf1 (Other)Mammalian Expression, CRISPRCpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas System. Cell. 2015 Sep 23. pii: S0092-8674(15)01200-3. doi: 10.1016/j.cell.2015.09.038. Expresses humanized EeCpf1 pcDNA3.1 Add to Cart
    69986pY014 (pcDNA3.1-hMbCpf1)hMbCpf1 (Other)Mammalian Expression, CRISPRCpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas System. Cell. 2015 Sep 23. pii: S0092-8674(15)01200-3. doi: 10.1016/j.cell.2015.09.038. Expresses humanized MbCpf1 pcDNA3.1 Add to Cart
    69987pY015 (pcDNA3.1-hLiCpf1)hLiCpf1 (Other)Mammalian Expression, CRISPRCpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas System. Cell. 2015 Sep 23. pii: S0092-8674(15)01200-3. doi: 10.1016/j.cell.2015.09.038. Expresses humanized LiCpf1 pcDNA3.1 Add to Cart
    69988pY016 (pcDNA3.1-hLbCpf1)hLbCpf1 (Other)Mammalian Expression, CRISPRCpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas System. Cell. 2015 Sep 23. pii: S0092-8674(15)01200-3. doi: 10.1016/j.cell.2015.09.038. Expresses humanized LbCpf1 pcDNA3.1 Add to Cart
    69989pY017 (pcDNA3.1-hPcCpf1)hPcCpf1 (Other)Mammalian Expression, CRISPRCpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas System. Cell. 2015 Sep 23. pii: S0092-8674(15)01200-3. doi: 10.1016/j.cell.2015.09.038. Expresses humanized PcCpf1 pcDNA3.1 Add to Cart
    69990pY018 (pcDNA3.1-hPdCpf1)hPdCpf1 (Other)Mammalian Expression, CRISPRCpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas System. Cell. 2015 Sep 23. pii: S0092-8674(15)01200-3. doi: 10.1016/j.cell.2015.09.038. Expresses humanized PdCpf1 pcDNA3.1 Add to Cart
    69991pY019 (pcDNA3.1-hPmCpf1)hPmCpf1 (Other)Mammalian Expression, CRISPRCpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas System. Cell. 2015 Sep 23. pii: S0092-8674(15)01200-3. doi: 10.1016/j.cell.2015.09.038. Expresses humanized PmCpf1 pcDNA3.1 Add to Cart
    70166pZ001 (AacC2c1 locus)AacC2c1 locus (Other)Bacterial ExpressionDiscovery and Functional Characterization of Diverse Class 2 CRISPR-Cas Systems. Mol Cell. 2015 Nov 5;60(3):385-97. doi: 10.1016/j.molcel.2015.10.008. Epub 2015 Oct 22. Expresses AacC2c1 full locus pACYC184 Add to Cart
    70168pZ003 (LshC2c2 locus)LshC2c2 full locus (Other)Bacterial ExpressionDiscovery and Functional Characterization of Diverse Class 2 CRISPR-Cas Systems. Mol Cell. 2015 Nov 5;60(3):385-97. doi: 10.1016/j.molcel.2015.10.008. Epub 2015 Oct 22. Expresses LshC2c2 full locus pACYC184 Add to Cart
    70170pZ004 (LseC2c2 locus)LseC2c2 locus with one spacer in array (Other)Bacterial ExpressionDiscovery and Functional Characterization of Diverse Class 2 CRISPR-Cas Systems. Mol Cell. 2015 Nov 5;60(3):385-97. doi: 10.1016/j.molcel.2015.10.008. Epub 2015 Oct 22. Expresses LseC2c2 locus with one spacer in array pET28a Add to Cart
    70171pZ005 (AacC2c1 human codon optimized protein)AacC2c1 protein (Other)Discovery and Functional Characterization of Diverse Class 2 CRISPR-Cas Systems. Mol Cell. 2015 Nov 5;60(3):385-97. doi: 10.1016/j.molcel.2015.10.008. Epub 2015 Oct 22. Expresses AacC2c1 human codon optimized protein BPV00356 pET21GG2-His(6)-MBP_Flag-Avi Add to Cart
    71814eSpCas9(1.1)enhanced specificity Cas9 (1.1) (Other)Mammalian Expression, CRISPRRationally engineered Cas9 nucleases with improved specificity. Science. 2015 Dec 1. pii: aad5227. Expresses high specificity SpCas9. Px330-like plasmid. pX330-U6-Chimeric_BB-CBh-hSpCas9 Add to Cart
    73795lenti sgRNA(MS2)_puro backboneMammalian Expression, Lentiviral, CRISPRGenome-scale transcriptional activation by an engineered CRISPR-Cas9 complex. Nature. 2014 Dec 10. doi: 10.1038/nature14136. lenti sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2 and EF1a-puro resistance marker. Contains BsmBI sites for insertion of spacer sequences. plenti Add to Cart
    73797lenti sgRNA(MS2)_puro optimized backboneMammalian Expression, Lentiviral, CRISPR lenti sgRNA(MS2)_puro optimized backbone (unpublished) optimized lenti sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2 and EF1a-puro resistance marker. Contains BsmBI sites for insertion of spacer sequences. pLKO Add to Cart