Feng Zhang Lab Plasmids


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Title Authors Publication
In vivo interrogation of gene function in the mammalian brain using CRISPR-Cas9. Swiech L, Heidenreich M, Banerjee A, Habib N, Li Y, Trombetta J, Sur M, Zhang F Nat Biotechnol. 2014 Oct 19. doi: 10.1038/nbt.3055.
CRISPR-Cas9 Knockin Mice for Genome Editing and Cancer Modeling. Platt RJ, Chen S, Zhou Y, Yim MJ, Swiech L, Kempton HR, Dahlman JE, Parnas O, Eisenhaure TM, Jovanovic M, Graham DB, Jhunjhunwala S, Heidenreich M, Xavier RJ, Langer R, Anderson DG, Hacohen N, Regev A, Feng G, Sharp PA, Zhang F Cell. 2014 Sep 24. pii: S0092-8674(14)01163-5. doi: 10.1016/j.cell.2014.09.014.
Improved vectors and genome-wide libraries for CRISPR screening. Sanjana NE, Shalem O, Zhang F Nat Methods. 2014 Aug;11(8):783-4. doi: 10.1038/nmeth.3047.
Crystal structure of Cas9 in complex with guide RNA and target DNA. Nishimasu H, Ran FA, Hsu PD, Konermann S, Shehata SI, Dohmae N, Ishitani R, Zhang F, Nureki O Cell. 2014 Feb 27;156(5):935-49. doi: 10.1016/j.cell.2014.02.001. Epub 2014 Feb 13.
Genome-scale CRISPR-Cas9 knockout screening in human cells. Shalem O, Sanjana NE, Hartenian E, Shi X, Scott DA, Mikkelsen TS, Heckl D, Ebert BL, Root DE, Doench JG, Zhang F Science. 2014 Jan 3;343(6166):84-7. doi: 10.1126/science.1247005. Epub 2013 Dec 12.
Genome engineering using the CRISPR-Cas9 system. Ran FA, Hsu PD, Wright J, Agarwala V, Scott DA, Zhang F Nat Protoc. 2013 Nov;8(11):2281-308. doi: 10.1038/nprot.2013.143. Epub 2013 Oct 24.
Optical control of mammalian endogenous transcription and epigenetic states. Konermann S, Brigham MD, Trevino A, Hsu PD, Heidenreich M, Le Cong, Platt RJ, Scott DA, Church GM, Zhang F Nature. 2013 Jul 23. doi: 10.1038/nature12466.
Multiplex Genome Engineering Using CRISPR/Cas Systems. Cong L, Ran FA, Cox D, Lin S, Barretto R, Habib N, Hsu PD, Wu X, Jiang W, Marraffini LA, Zhang F Science. 2013 Jan 3.
Comprehensive interrogation of natural TALE DNA-binding modules and transcriptional repressor domains. Cong L, Zhou R, Kuo YC, Cunniff M, Zhang F Nat Commun. 2012 Jul 24;3:968. doi: 10.1038/ncomms1962.
A transcription activator-like effector toolbox for genome engineering. Sanjana NE, Cong L, Zhou Y, Cunniff MM, Feng G, Zhang F Nat Protoc. 2012 Jan 5;7(1):171-92. doi: 10.1038/nprot.2011.431.
Efficient construction of sequence-specific TAL effectors for modulating mammalian transcription. Zhang F, Cong L, Lodato S, Kosuri S, Church GM, Arlotta P Nat Biotechnol. 2011 Jan 19. ():.
TALE Nuclease (TALEN) (unpublished)
ID Plasmid Gene/Insert Vector Type Publication Hidden Extra Search Info  
27961 pLenti-EF1a-Backbone(NN) TAL-Backbone(NN) (Synthetic) Mammalian Expression, Lentiviral Zhang et al Nat Biotechnol. 2011 Jan 19. ():. pLenti
27962 pLenti-EF1a-Backbone(NI) TAL-Backbone(NI) (Synthetic) Mammalian Expression, Lentiviral Zhang et al Nat Biotechnol. 2011 Jan 19. ():. pLenti
27963 pLenti-EF1a-Backbone(NG) TAL-Backbone(NG) (Synthetic) Mammalian Expression, Lentiviral Zhang et al Nat Biotechnol. 2011 Jan 19. ():. pLenti
27964 pLenti-EF1a-Backbone(HD) TAL-Backbone(HD) (Synthetic) Mammalian Expression, Lentiviral Zhang et al Nat Biotechnol. 2011 Jan 19. ():. pLenti
27965 monomer-NN TAL-monomer-NN Bacterial Expression Zhang et al Nat Biotechnol. 2011 Jan 19. ():. pJ201
27966 monomer-NI TAL-monomer-NI Bacterial Expression Zhang et al Nat Biotechnol. 2011 Jan 19. ():. pJ201
27967 monomer-NG TAL-monomer-NG Bacterial Expression Zhang et al Nat Biotechnol. 2011 Jan 19. ():. pJ201
27968 monomer-HD TAL-monomer-HD Bacterial Expression Zhang et al Nat Biotechnol. 2011 Jan 19. ():. pJ201
27969 pLenti-EF1a-TALE1 TALE1 (Synthetic) Mammalian Expression, Lentiviral Zhang et al Nat Biotechnol. 2011 Jan 19. ():. pLenti
27970 pAAV-minCMV-mCherry mCherry (Synthetic) Mammalian Expression Zhang et al Nat Biotechnol. 2011 Jan 19. ():. pAAV
31178 Monomer NK Monomer-NK Bacterial Expression TALE Nuclease (TALEN) (unpublished) pJ201
31179 TALEN Backbone TALE Nuclease Backbone Mammalian Expression TALE Nuclease (TALEN) (unpublished) pCMV
32180 pNI_v2 TALE Monomer NI Bacterial Expression Sanjana et al Nat Protoc. 2012 Jan 5;7(1):171-92. doi: 10.1038/nprot.2011.431. pJ201
32181 pNG_v2 TALE Monomer NG Bacterial Expression Sanjana et al Nat Protoc. 2012 Jan 5;7(1):171-92. doi: 10.1038/nprot.2011.431. pJ201
32182 pNN_v2 TALE Monomer NN Bacterial Expression Sanjana et al Nat Protoc. 2012 Jan 5;7(1):171-92. doi: 10.1038/nprot.2011.431. pJ201
32183 pHD_v2 TALE Monomer HD Bacterial Expression Sanjana et al Nat Protoc. 2012 Jan 5;7(1):171-92. doi: 10.1038/nprot.2011.431. pJ201
32185 pTALETF_v2 (NN) TALE Transcriptional Activator Backbone, NN for 0.5 repeat Mammalian Expression Sanjana et al Nat Protoc. 2012 Jan 5;7(1):171-92. doi: 10.1038/nprot.2011.431. pCMV
32186 pTALETF_v2 (NG) TALE Transcriptional Activator Backbone, NG for 0.5 repeat Mammalian Expression Sanjana et al Nat Protoc. 2012 Jan 5;7(1):171-92. doi: 10.1038/nprot.2011.431. pCMV
32187 pTALETF_v2 (NI) TALE Transcriptional Activator Backbone, NI for 0.5 repeat Mammalian Expression Sanjana et al Nat Protoc. 2012 Jan 5;7(1):171-92. doi: 10.1038/nprot.2011.431. pCMV
32188 pTALETF_v2 (HD) TALE Transcriptional Activator Backbone, HD for 0.5 repeat Mammalian Expression Sanjana et al Nat Protoc. 2012 Jan 5;7(1):171-92. doi: 10.1038/nprot.2011.431. pCMV
32189 pTALEN_v2 (NI) TALE Nuclease, NI for 0.5 repeat Mammalian Expression Sanjana et al Nat Protoc. 2012 Jan 5;7(1):171-92. doi: 10.1038/nprot.2011.431. pCMV
32190 pTALEN_v2 (NG) TALE Nuclease, NG for 0.5 repeat Mammalian Expression Sanjana et al Nat Protoc. 2012 Jan 5;7(1):171-92. doi: 10.1038/nprot.2011.431. pCMV
32191 pTALEN_v2 (NN) TALE Nuclease, NN for 0.5 repeat Mammalian Expression Sanjana et al Nat Protoc. 2012 Jan 5;7(1):171-92. doi: 10.1038/nprot.2011.431. pCMV
32192 pTALEN_v2 (HD) TALE Nuclease, HD for 0.5 repeat Mammalian Expression Sanjana et al Nat Protoc. 2012 Jan 5;7(1):171-92. doi: 10.1038/nprot.2011.431. pCMV
35388 pLenti-EF1a-SOX2 TALE-Sox2 (Synthetic) Mammalian Expression, Lentiviral Zhang et al Nat Biotechnol. 2011 Jan 19. ():. pLenti
35389 pLenti-EF1a-KLF4 TALE-Klf4 (Synthetic) Mammalian Expression, Lentiviral Zhang et al Nat Biotechnol. 2011 Jan 19. ():. pLenti
35391 pLenti-EF1a-OCT4 TALE-Oct4 (Synthetic) Mammalian Expression, Lentiviral Zhang et al Nat Biotechnol. 2011 Jan 19. ():. pLenti
35420 TALE5 TALE5 (Synthetic) Mammalian Expression, Lentiviral Zhang et al Nat Biotechnol. 2011 Jan 19. ():. pLenti
  • Tag / Fusion Protein
    • EGFP (C terminal on insert)
  • 35421 TALE6 TALE6 (Synthetic) Mammalian Expression, Lentiviral Zhang et al Nat Biotechnol. 2011 Jan 19. ():. pLenti
  • Tag / Fusion Protein
    • EGFP (C terminal on insert)
  • 35423 TALE8 TALE8 (Synthetic) Mammalian Expression, Lentiviral Zhang et al Nat Biotechnol. 2011 Jan 19. ():. pLenti
  • Tag / Fusion Protein
    • EGFP (C terminal on insert)
  • 35425 TALE10 TALE10 (Synthetic) Mammalian Expression, Lentiviral Zhang et al Nat Biotechnol. 2011 Jan 19. ():. pLenti
  • Tag / Fusion Protein
    • EGFP (C terminal on insert)
  • 35426 TALE11 TALE11 (Synthetic) Mammalian Expression, Lentiviral Zhang et al Nat Biotechnol. 2011 Jan 19. ():. pLenti
    35427 TALE12 TALE12 (Synthetic) Mammalian Expression, Lentiviral Zhang et al Nat Biotechnol. 2011 Jan 19. ():. pLenti
  • Tag / Fusion Protein
    • EGFP (C terminal on insert)
  • 35431 hAAVS1 1L TALEN hAAVS1 1L TALEN Mammalian Expression Sanjana et al Nat Protoc. 2012 Jan 5;7(1):171-92. doi: 10.1038/nprot.2011.431. TALEN designed to target the human AAVS1 locus TALEN BB
    35432 hAAVS1 1R TALEN hAAVS1 1R TALEN Mammalian Expression Sanjana et al Nat Protoc. 2012 Jan 5;7(1):171-92. doi: 10.1038/nprot.2011.431. TALEN designed to target the human AAVS1 locus TALEN BB
    37527 Monomer NH_V2 Monomer NH (Synthetic) Cong et al Nat Commun. 2012 Jul 24;3:968. doi: 10.1038/ncomms1962. template for PCR amplification of the monomer used for assembly of the DNA-binding tandem repeat region of the TALE. PJ201
    37529 Monomer NA_V2 Monomer NA (Synthetic) Cong et al Nat Commun. 2012 Jul 24;3:968. doi: 10.1038/ncomms1962. PJ201
    41863 pX097-U6-SpTracrRNA-EF1a-hSpRNaseIII-mCherry U6-SpTracrRNA-EF1a-hSpRNaseIII-mCherry Mammalian Expression, Lentiviral, CRISPR Cong et al Science. 2013 Jan 3. This plasmid is used to reconstitute the complete Type II CRISPR system from S. pyogenes and contains the host factor RNaseIII and tracrRNA. pLenti
    42229 pX260-U6-DR-BB-DR-Cbh-NLS-hSpCas9-NLS-H1-shorttracr-PGK-puro humanized S. pyogenes Cas9 Mammalian Expression, CRISPR Cong et al Science. 2013 Jan 3. This plasmid separately encodes a human codon-optimized SpCas9, a tracrRNA and customizable crRNA. pUC ori vector
    42230 pX330-U6-Chimeric_BB-CBh-hSpCas9 humanized S. pyogenes Cas9 Mammalian Expression, CRISPR Cong et al Science. 2013 Jan 3. A human codon-optimized SpCas9 and chimeric guide RNA expression plasmid. pUC ori vector
  • Tag / Fusion Protein
    • 3xFLAG (N terminal on insert)
  • 42333 pX334-U6-DR-BB-DR-Cbh-NLS-hSpCas9n(D10A)-NLS-H1-shorttracr-PGK-puro humanized S. pyogenes Cas9 (D10A) nickase Mammalian Expression, CRISPR Cong et al Science. 2013 Jan 3. This plasmid separately encodes a human codon-optimized SpCas9 nickase, a tracrRNA and customizable crRNA. pUC ori vector
  • Tag / Fusion Protein
    • HA (N terminal on insert)
  • 42335 pX335-U6-Chimeric_BB-CBh-hSpCas9n(D10A) humanized S. pyogenes Cas9 (D10A) nickase Mammalian Expression, CRISPR Cong et al Science. 2013 Jan 3. A human codon-optimized SpCas9 nickase and chimeric guide RNA expression plasmid. pUC ori vector
  • Tag / Fusion Protein
    • HA (N terminal on insert)
  • 42337 pX261-U6-DR-hEmx1-DR-Cbh-NLS-hSpCas9-NLS-H1-shorttracr-PGK-puro humanized S. pyogenes Cas9 Mammalian Expression, CRISPR Cong et al Science. 2013 Jan 3. Dual expression plasmid of human codon-optimized SpCas9 and a gRNA to the human Emx1 locus, can be used to test SpCas9 cleavage in cell lines of choice. pUC ori vector
  • Tag / Fusion Protein
    • 3xFLAG (N terminal on insert)
  • 42581 pAAV_TALE-TF(VP64)-BB_V3 TALE-TF(VP64)-BB Mammalian Expression ; Adeno-associated Virus (AAV) Cong et al Nat Commun. 2012 Jul 24;3:968. doi: 10.1038/ncomms1962. pAAV
    42588 pAAV_TALEN-FokI(WT)-BB_V3 TALEN-FokI(WT)-BB Mammalian Expression ; Adeno-associated Virus (AAV) Cong et al Nat Commun. 2012 Jul 24;3:968. doi: 10.1038/ncomms1962. pAAV
  • Tag / Fusion Protein
    • 3XFlag (N terminal on backbone)
  • 42945 SOX2 TALE Repressor (KRAB 1-75) SOX2 TALE Repressor (KRAB 1-75) Mammalian Expression, Lentiviral Cong et al Nat Commun. 2012 Jul 24;3:968. doi: 10.1038/ncomms1962. pLenti
  • Tag / Fusion Protein
    • 2A-GFP (C terminal on insert)
  • 42946 SOX2 TALE Repressor (KRAB 11-75) SOX2 TALE Repressor (KRAB 11-75) Mammalian Expression, Lentiviral Cong et al Nat Commun. 2012 Jul 24;3:968. doi: 10.1038/ncomms1962. pLenti
  • Tag / Fusion Protein
    • 2A-GFP (C terminal on insert)
  • 43882 Enhanced Repressor Domain for TAL Effector (SID4X) SID4X Domain Cong et al Nat Commun. 2012 Jul 24;3:968. doi: 10.1038/ncomms1962. pUC57
    47445 pAAV_hSyn_TALEBB(NN)-NLS-VP64_2A_GFP_WPRE_bGHpA TALE BB(N136_0.5NN_C63)-VP64 (Synthetic) Mammalian Expression, AAV ; TALE Konermann et al Nature. 2013 Jul 23. doi: 10.1038/nature12466. AAV TALE synthesis backbone with NN half repeat; fused to nuclear localization sequence linker and VP64 transcriptional activator effector domain. Synapsin promoter for neuronal expression. pAAV_hSyn_WPRE_bGHpA
    47446 pAAV_hSyn_TALEBB(HD)-NLS-VP64_2A_GFP_WPRE_bGHpA TALE BB(N136_0.5HD_C63)-VP64 (Synthetic) Mammalian Expression, AAV ; TALE Konermann et al Nature. 2013 Jul 23. doi: 10.1038/nature12466. AAV TALE synthesis backbone with HD half repeat; fused to nuclear localization sequence linker and VP64 transcriptional activator effector domain. Synapsin promoter for neuronal expression. pAAV_hSyn_WPRE_bGHpA
    47447 pAAV_hSyn_TALEBB(NG)-NLS-VP64_2A_GFP_WPRE_bGHpA TALE BB(N136_0.5NG_C63)-VP64 (Synthetic) Mammalian Expression, AAV ; TALE Konermann et al Nature. 2013 Jul 23. doi: 10.1038/nature12466. AAV TALE synthesis backbone with NG half repeat; fused to nuclear localization sequence linker and VP64 transcriptional activator effector domain. Synapsin promoter for neuronal expression. pAAV_hSyn_WPRE_bGHpA
    47448 pAAV_hSyn_TALEBB(NI)-NLS-VP64_2A_GFP_WPRE_bGHpA TALE BB(N136_0.5NI_C63)-VP64 (Synthetic) Mammalian Expression, AAV ; TALE Konermann et al Nature. 2013 Jul 23. doi: 10.1038/nature12466. AAV TALE synthesis backbone with NI half repeat; fused to nuclear localization sequence linker and VP64 transcriptional activator effector domain. Synapsin promoter for neuronal expression. pAAV_hSyn_WPRE_bGHpA
    47449 pAAV_hSyn_TALEBB(NN)-NLS-SID4X_2A_phiLOV2.1_WPRE_bGHpA TALE BB(N136_0.5NN_C63)-SID4X (Synthetic) Mammalian Expression, AAV ; TALE Konermann et al Nature. 2013 Jul 23. doi: 10.1038/nature12466. AAV TALE synthesis backbone with NN half repeat; fused to nuclear localization sequence linker and SID4X transcriptional repressor effector domain. Synapsin promoter for neuronal expression. pAAV_hSyn_WPRE_bGHpA
    47450 pAAV_hSyn_TALEBB(HD)-NLS-SID4X_2A_phiLOV2.1_WPRE_bGHpA TALE BB(N136_0.5HD_C63)-SID4X (Synthetic) Mammalian Expression, AAV ; TALE Konermann et al Nature. 2013 Jul 23. doi: 10.1038/nature12466. AAV TALE synthesis backbone with HD half repeat; fused to nuclear localization sequence linker and SID4X transcriptional repressor effector domain. Synapsin promoter for neuronal expression. pAAV_hSyn_WPRE_bGHpA
    47451 pAAV_hSyn_TALEBB(NG)-NLS-SID4X_2A_phiLOV2.1_WPRE_bGHpA TALE BB(N136_0.5NG_C63)-SID4X (Synthetic) Mammalian Expression, AAV ; TALE Konermann et al Nature. 2013 Jul 23. doi: 10.1038/nature12466. AAV TALE synthesis backbone with NG half repeat; fused to nuclear localization sequence linker and SID4X transcriptional repressor effector domain. Synapsin promoter for neuronal expression. pAAV_hSyn_WPRE_bGHpA
    47452 pAAV_hSyn_TALEBB(NI)-NLS-SID4X_2A_phiLOV2.1_WPRE_bGHpA TALE BB(N136_0.5NI_C63)-SID4X (Synthetic) Mammalian Expression, AAV ; TALE Konermann et al Nature. 2013 Jul 23. doi: 10.1038/nature12466. AAV TALE synthesis backbone with NI half repeat; fused to nuclear localization sequence linker and SID4X transcriptional repressor effector domain. Synapsin promoter for neuronal expression. pAAV_hSyn_WPRE_bGHpA
    47453 LITE1.0_pAAV_hSyn_TALE(Grm2)-NLS-CIB1_2A_GFP_WPRE_bGHpA TALE (N136,Grm2,C63)-cib1 (Synthetic) Mammalian Expression, AAV ; TALE Konermann et al Nature. 2013 Jul 23. doi: 10.1038/nature12466. epiLITE / LITE1.0 TALE-CIB1. CIB1 binds to blue-light-activated CRY2PHR. Particular TALE targeted to Grm2 promoter. Synapsin promoter for neuronal expression. pAAV_hSyn_WPRE_bGHpA
    47454 LITE1.0_pAAV_hSyn_CRY2PHR-NLS-SID4X_2A_phiLOV2.1_WPRE_bGHpA CRY2PHR-NLS-SID4X (Synthetic) Mammalian Expression, AAV Konermann et al Nature. 2013 Jul 23. doi: 10.1038/nature12466. epiLITE / LITE1.0 CRY2PHR fused to SID4X transcriptional repressor effector domain. Binds to CIB1 upon blue light stimulation. Synapsin promoter for neuronal expression. pAAV_hSyn_WPRE_bGHpA
    47455 LITE2.0 pAAV_hSyn_NLS(alpha-imp)-CRY2PHR-NLS-VP64_2A_GFP_WPRE_bGHpA NLS(alpha-imp)-CRY2PHR-NLS-VP64 (Synthetic) Mammalian Expression, AAV Konermann et al Nature. 2013 Jul 23. doi: 10.1038/nature12466. LITE2.0 CRY2PHR-VP64. Changes from LITE1.0: N-terminal alpha-importin nuclear localization sequence. Synapsin promoter for neuronal expression. pAAV_hSyn_WPRE_bGHpA
    47456 LITE2.0_pAAV_hSyn_TALE(Grm2)-GS-CIB1(NLS*,∆318-334)_WPRE_bGHpA TALE(Grm2)-GS-CIB1(NLS*,∆318-334) (Synthetic) Mammalian Expression, AAV ; TALE Konermann et al Nature. 2013 Jul 23. doi: 10.1038/nature12466. LITE2.0 TALE-CIB1. Changes from LITE1.0: glycine-serine linker, mutated endogenous NLS, aa318-334 deletion. This particular TALE targeted to Grm2. Synapsin promoter for neuronal expression. pAAV_hSyn_WPRE_bGHpA
    47457 LITE2.0 EF1a_NLS(alpha-imp)-CRY2PHR-NLS-VP64_2A_GFP_WPRE_hGHpA NLS(alpha-imp)-CRY2PHR-NLS-VP64 (Synthetic) Mammalian Expression Konermann et al Nature. 2013 Jul 23. doi: 10.1038/nature12466. LITE2.0 CRY2PHR-VP64. Changes from LITE1.0: N-terminal alpha-importin nuclear localization sequence. EF1-alpha promoter for ubiquitous mammalian expression. pAAV_EF1a_WPRE_hGHpA
    47458 LITE2.0 EF1a_TALE(Neurog2)-GS-CIB1(NLS*,∆318-334)_WPRE_hGHpA TALE (Neurog2)-GS-CIB1(NLS*,∆318-334) (Synthetic) Mammalian Expression Konermann et al Nature. 2013 Jul 23. doi: 10.1038/nature12466. LITE2.0 TALE-CIB1. Changes from LITE1.0: glycine-serine linker, mutated endogenous NLS, aa318-334 deletion. This particular TALE targeted to Neurog2. EF1-alpha promoter for ubiquitous mammalian exp. pAAV_EF1a_WPRE_hGHpA
    48137 pSpCas9 (PX165) hSpCas9 (Synthetic) Mammalian Expression, CRISPR Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi: 10.1038/nprot.2013.143. Epub 2013 Oct 24. Human codon optimized Cas9 nuclease from Streptococcus pyogenes (Cbh-3X-FLAG-NLS-SpCas9-NLS) PX165
    48138 pSpCas9(BB)-2A-GFP (PX458) hSpCas9 (Synthetic) CRISPR Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi: 10.1038/nprot.2013.143. Epub 2013 Oct 24. Cas9 from S. pyogenes with 2A-EGFP, and cloning backbone for sgRNA PX458
    48139 pSpCas9(BB)-2A-Puro (PX459) hSpCas9-2A-Puro (Synthetic) CRISPR Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi: 10.1038/nprot.2013.143. Epub 2013 Oct 24. Cas9 from S. pyogenes with 2A-Puro, and cloning backbone for sgRNA PX459
    48140 pSpCas9n(BB)-2A-GFP (PX461) hSpCas9n (Synthetic) CRISPR Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi: 10.1038/nprot.2013.143. Epub 2013 Oct 24. Cas9n (D10A nickase mutant) from S. pyogenes with 2A-EGFP, and cloning backbone for sgRNA PX461
    48141 pSpCas9n(BB)-2A-Puro (PX462) hSpCas9n-2A-Puro (Synthetic) CRISPR Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi: 10.1038/nprot.2013.143. Epub 2013 Oct 24. Cas9n (D10A nickase mutant) from S. pyogenes with 2A-Puro, and cloning backbone for sgRNA PX462
    48253 LITE1.0_pAAV_hSyn_CRY2PHR-NLS-VP64_2A_GFP_WPRE_bGHpA CRY2PHR-NLS-VP64 (Synthetic) Mammalian Expression, AAV ; TALE Konermann et al Nature. 2013 Jul 23. doi: 10.1038/nature12466. LITE1.0 CRY2PHR fused to VP64 transcriptional activator domain. Binds to CIB1 upon blue light stimulation. Synapsin promoter for neuronal expression. See LITE2.0 for optimized LITE activators. pAAV_hSyn_WPRE_bGHpA
    48873 pSpCas9n(BB) (PX460) hSpCas9n (Synthetic) Mammalian Expression, CRISPR Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi: 10.1038/nprot.2013.143. Epub 2013 Oct 24. Cas9n (D10A nickase mutant) from S. pyogenes PX460
    51760 lentiCRISPR - EGFP sgRNA 1 Cas9 (Synthetic), Puromycin resistance (Other), EGFP sgRNA 1 (Synthetic) Mammalian Expression, Lentiviral, CRISPR Shalem et al Science. 2014 Jan 3;343(6166):84-7. doi: 10.1126/science.1247005. Epub 2013 Dec 12. Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an EGFP targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone. lentiCRISPR (pXPR_001), Addgene plasmid #49535
    51761 lentiCRISPR - EGFP sgRNA 2 Cas9 (Synthetic), Puromycin resistance (Other), EGFP sgRNA 2 (Synthetic) Mammalian Expression, Lentiviral, CRISPR Shalem et al Science. 2014 Jan 3;343(6166):84-7. doi: 10.1126/science.1247005. Epub 2013 Dec 12. Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an EGFP targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone. lentiCRISPR (pXPR_001), Addgene plasmid #49535
    51762 lentiCRISPR - EGFP sgRNA 3 Cas9 (Synthetic), Puromycin resistance (Other), EGFP sgRNA 3 (Synthetic) Mammalian Expression, Lentiviral, CRISPR Shalem et al Science. 2014 Jan 3;343(6166):84-7. doi: 10.1126/science.1247005. Epub 2013 Dec 12. Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an EGFP targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone. lentiCRISPR (pXPR_001), Addgene plasmid #49535
    51763 lentiCRISPR - EGFP sgRNA 4 Cas9 (Synthetic), Puromycin resistance (Other), EGFP sgRNA 4 (Synthetic) Mammalian Expression, Lentiviral, CRISPR Shalem et al Science. 2014 Jan 3;343(6166):84-7. doi: 10.1126/science.1247005. Epub 2013 Dec 12. Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an EGFP targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone. lentiCRISPR (pXPR_001), Addgene plasmid #49535
    51764 lentiCRISPR - EGFP sgRNA 5 Cas9 (Synthetic), Puromycin resistance (Other), EGFP sgRNA 5 (Synthetic) Lentiviral, CRISPR Shalem et al Science. 2014 Jan 3;343(6166):84-7. doi: 10.1126/science.1247005. Epub 2013 Dec 12. Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an EGFP targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone. lentiCRISPR (pXPR_001), Addgene plasmid #49535
    51765 lentiCRISPR - EGFP sgRNA 6 Cas9 (Synthetic), Puromycin resistance (Other), EGFP sgRNA 6 (Synthetic) Mammalian Expression, Lentiviral, CRISPR Shalem et al Science. 2014 Jan 3;343(6166):84-7. doi: 10.1126/science.1247005. Epub 2013 Dec 12. Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an EGFP targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone. lentiCRISPR (pXPR_001), Addgene plasmid #49535
    52961 lentiCRISPR v2 Cas9 (Synthetic), Puromycin resistance Lentiviral, CRISPR Sanjana et al Nat Methods. 2014 Aug;11(8):783-4. doi: 10.1038/nmeth.3047. Replaces original lentiCRISPRv1 (Addgene Plasmid 49535) and produces ~10-fold higher titer virus. Custom
    52962 lentiCas9-Blast Cas9 (Synthetic), Blasticidin resistance Mammalian Expression, Lentiviral, CRISPR Sanjana et al Nat Methods. 2014 Aug;11(8):783-4. doi: 10.1038/nmeth.3047. Expresses human codon-optimized S. pyogenes Cas9 protein and blasticidin resistance from EFS promoter. Lentiviral backbone. pFUGW
    52963 lentiGuide-Puro S. pyogenes sgRNA cassette (Synthetic), Puromycin resistance Lentiviral, CRISPR Sanjana et al Nat Methods. 2014 Aug;11(8):783-4. doi: 10.1038/nmeth.3047. Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and puromycin resistance from EF-1a promoter. Lentiviral backbone. Custom
    58888 PX543 SpCas9(del175-307) hSpCas9 (Synthetic) Mammalian Expression, CRISPR Nishimasu et al Cell. 2014 Feb 27;156(5):935-49. doi: 10.1016/j.cell.2014.02.001. Epub 2014 Feb 13. Truncated form of SpCas9 without REC2 domain PX165
    58889 PX544 SpCas9(del1098-1368) hSpCas9 (Synthetic) Mammalian Expression, CRISPR Nishimasu et al Cell. 2014 Feb 27;156(5):935-49. doi: 10.1016/j.cell.2014.02.001. Epub 2014 Feb 13. Truncated form of SpCas9 without C' domain PX165
    60224 AAV:ITR-U6-sgRNA(Kras)-U6-sgRNA(p53)-U6-sgRNA(Lkb1)-pEFS-Rluc-2A-Cre-shortPA-KrasG12D_HDRdonor-ITR (AAV-KPL) sgRNA (Synthetic), Renilla luciferase, Cre recombinase, KrasG12D HDR donor Mammalian Expression, Mouse Targeting, AAV, Cre/Lox, CRISPR Platt et al Cell. 2014 Sep 24. pii: S0092-8674(14)01163-5. doi: 10.1016/j.cell.2014.09.014. Expresses Cre recombinase and Renilla luciferase from the EFS promoter and three U6-driven sgRNAs targeting Kras, p53, and Lkb1. Contains KrasG12D HDR donor. AAV backbone. AAV
    60225 AAV:ITR-U6-sgRNA(LacZ)-pEFS-Rluc-2A-Cre-WPRE-hGHpA-ITR sgRNA, Renilla luciferase, Cre recombinase Mammalian Expression, Mouse Targeting, AAV, Cre/Lox, CRISPR, Luciferase Platt et al Cell. 2014 Sep 24. pii: S0092-8674(14)01163-5. doi: 10.1016/j.cell.2014.09.014. Expresses Cre recombinase and Renilla luciferase from the EFS promoter and one U6-driven sgRNA targeting LacZ. AAV backbone. AAV
    60226 AAV:ITR-U6-sgRNA(backbone)-pEFS-Rluc-2A-Cre-WPRE-hGHpA-ITR sgRNA, Renilla luciferase, Cre recombinase Mammalian Expression, Mouse Targeting, AAV, Cre/Lox, TALEN, Luciferase Platt et al Cell. 2014 Sep 24. pii: S0092-8674(14)01163-5. doi: 10.1016/j.cell.2014.09.014. Expresses Cre recombinase and Renilla luciferase from the EFS promoter and one U6-driven sgRNA. AAV backbone with SapI spacer for sgRNA cloning. AAV
    60227 AAV:ITR-U6-sgRNA(NeuN)-pCBh-Cre-WPRE-hGHpA-ITR sgRNA, Cre recombinase Mammalian Expression, Mouse Targeting, AAV, Cre/Lox, CRISPR Platt et al Cell. 2014 Sep 24. pii: S0092-8674(14)01163-5. doi: 10.1016/j.cell.2014.09.014. Expresses Cre recombinase from the Cbh promoter and one U6-driven sgRNA targeting the mouse gene NeuN. AAV backbone. AAV
    60228 AAV:ITR-U6-sgRNA(LacZ)-pCBh-Cre-WPRE-hGHpA-ITR sgRNA, Cre recombinase Mammalian Expression, Mouse Targeting, AAV, Cre/Lox, CRISPR Platt et al Cell. 2014 Sep 24. pii: S0092-8674(14)01163-5. doi: 10.1016/j.cell.2014.09.014. Expresses Cre recombinase from the Cbh promoter and one U6-driven sgRNA control targeting LacZ. AAV backbone. AAV
    60229 AAV:ITR-U6-sgRNA(backbone)-pCBh-Cre-WPRE-hGHpA-ITR sgRNA, Cre recombinase Mammalian Expression, Mouse Targeting, AAV, Cre/Lox, CRISPR Platt et al Cell. 2014 Sep 24. pii: S0092-8674(14)01163-5. doi: 10.1016/j.cell.2014.09.014. Expresses Cre recombinase from the Cbh promoter and one U6-driven sgRNA. AAV backbone with SapI spacer for sgRNA cloning. AAV
    60230 AAV:ITR-U6-sgRNA(NeuN)-hSyn-Cre-2A-EGFP-KASH-WPRE-shortPA-ITR sgRNA, Cre recombinase, EGFP Mammalian Expression, Mouse Targeting, AAV, Cre/Lox, CRISPR Platt et al Cell. 2014 Sep 24. pii: S0092-8674(14)01163-5. doi: 10.1016/j.cell.2014.09.014. Expresses Cre recombinase and KASH-tagged EGFP from the hSyn promoter and one U6-driven sgRNA. AAV backbone with SapI spacer for sgRNA cloning. AAV
    60231 AAV:ITR-U6-sgRNA(backbone)-hSyn-Cre-2A-EGFP-KASH-WPRE-shortPA-ITR sgRNA, Cre recombinase, EGFP Mammalian Expression, Mouse Targeting, AAV, Cre/Lox, CRISPR Platt et al Cell. 2014 Sep 24. pii: S0092-8674(14)01163-5. doi: 10.1016/j.cell.2014.09.014. Expresses Cre recombinase and KASH-tagged EGFP from the hSyn promoter and one U6-driven sgRNA. AAV backbone with SapI spacer for sgRNA cloning. AAV
    60957 PX551 SpCas9 (Synthetic) Mammalian Expression, AAV, CRISPR Swiech et al Nat Biotechnol. 2014 Oct 19. doi: 10.1038/nbt.3055. pAAV-pMecp2-SpCas9-spA (AAV-SpCas9). AAV plasmid expressing Cas9 in neurons under control of truncated mecp2 promoter. pAAV
    60958 PX552 U6_(SpaI)_sgRNA (Synthetic), Syn_EGFP-KASH (Synthetic) Mammalian Expression, Mouse Targeting, AAV, CRISPR Swiech et al Nat Biotechnol. 2014 Oct 19. doi: 10.1038/nbt.3055. pAAV-U6sgRNA(SapI)_hSyn-GFP-KASH-bGH (SpGuide acceptor). AAV plasmid for sgRNA cloning. GFP-KASH fusion facilitates FACS sorting of cells and nuclei. pAAV