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DR274
(Plasmid #42250)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 42250 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    Zebrafish-gRNA-ExpressionVector
  • Backbone manufacturer
    Joung lab DR274
  • Backbone size (bp) 2147
  • Vector type
    Worm Expression, CRISPR ; zebrafish expression
  • Promoter T7

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    XL1 Blue
  • Copy number
    Unknown

Cloning Information

Resource Information

Depositor Comments

For more information on Joung Lab CRISPR Plasmids please refer to: http://www.addgene.org/crispr/jounglab/

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    DR274 was a gift from Keith Joung (Addgene plasmid # 42250)
  • For your References section:

    Efficient genome editing in zebrafish using a CRISPR-Cas system. Hwang WY, Fu Y, Reyon D, Maeder ML, Tsai SQ, Sander JD, Peterson RT, Yeh JR, Joung JK. Nat Biotechnol. 2013 Jan 29. doi: 10.1038/nbt.2501. 10.1038/nbt.2501 PubMed 23360964