|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||47929||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4100
- Total vector size (bp) 8300
Vector typeMammalian Expression, CRISPR
Growth in Bacteria
Copy numberHigh Copy
Insert Size (bp)4200
- Promoter CMV
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site XbaI (not destroyed)
- 5′ sequencing primer SP6
- 3′ sequencing primer M13 Rev (Common Sequencing Primers)
Note from depositor: Although this plasmid produces smaller transcripts in addition to the expected one, the product mixture displays activity comparable to that from pT3TS-nCas9n.
For more information on Chen and Wente Lab CRISPR Plasmids please refer to: http://www.addgene.org/crispr/Chen/
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCS2-nCas9n was a gift from Wenbiao Chen (Addgene plasmid # 47929 ; http://n2t.net/addgene:47929 ; RRID:Addgene_47929)
For your References section:Efficient multiplex biallelic zebrafish genome editing using a CRISPR nuclease system. Jao LE, Wente SR, Chen W. Proc Natl Acad Sci U S A. 2013 Aug 5. 10.1073/pnas.1308335110 PubMed 23918387