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John Calarco Lab Plasmids


The John Calarco Lab has deposited plasmids at Addgene for distribution to the research community. Addgene is a nonprofit plasmid repository dedicated to improving life science research.

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Title Authors Publication
Heritable genome editing in C. elegans via a CRISPR-Cas9 system. Friedland AE, Tzur YB, Esvelt KM, Colaiacovo MP, Church GM, Calarco JA Nat Methods. 2013 Jun 30. doi: 10.1038/nmeth.2532.
ID Plasmid Gene/Insert Vector Type Publication Hidden Extra Search Info  
46168Peft-3::cas9-SV40_NLS::tbb-2 3'UTRcodon optimized Cas9_SV40 NLS with intron (Synthetic)CRISPRHeritable genome editing in C. elegans via a CRISPR-Cas9 system. Nat Methods. 2013 Jun 30. doi: 10.1038/nmeth.2532. pUC57 Add to Cart
46169PU6::unc-119_sgRNAunc-119 targeting sgRNA (Synthetic)CRISPRHeritable genome editing in C. elegans via a CRISPR-Cas9 system. Nat Methods. 2013 Jun 30. doi: 10.1038/nmeth.2532. pUC57 unc-119 CELE_M142.1 Add to Cart
46170PU6::klp-12_sgRNAklp-12 targeting sgRNA (Synthetic)Worm Expression, CRISPRHeritable genome editing in C. elegans via a CRISPR-Cas9 system. Nat Methods. 2013 Jun 30. doi: 10.1038/nmeth.2532. pUC57 klp-12 CELE_T01G1.1a Add to Cart