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Mike Boxem Lab Plasmids


The Mike Boxem Lab has deposited plasmids at Addgene for distribution to the research community. Addgene is a nonprofit plasmid repository dedicated to improving life science research.

Learn more about research in the Mike Boxem Lab.

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Title Authors Publication
CRISPR/Cas9-Targeted Mutagenesis in Caenorhabditis elegans. Waaijers S, Portegijs V, Kerver J, Lemmens BB, Tijsterman M, van den Heuvel S, Boxem M Genetics. 2013 Aug 26.
Boxem lab BirA and Avi-tag plasmids (unpublished)
Boxem lab CRISPR plasmids (unpublished)
ID Plasmid Gene/Insert Vector Type Publication Hidden Extra Search Info  
47941pMB60synthetic guide RNA (Synthetic)Worm Expression, CRISPRCRISPR/Cas9-Targeted Mutagenesis in Caenorhabditis elegans. Genetics. 2013 Aug 26. Can be used to generate synthetic guide in vitro from T7 promotor pMK Add to Cart
47943pMB70guide RNA (Synthetic)Worm Expression, CRISPRCRISPR/Cas9-Targeted Mutagenesis in Caenorhabditis elegans. Genetics. 2013 Aug 26. Can be used to express a CRISPR guide RNA in C. elegans from U6 regulatory sequences pMK Add to Cart
47944pMB62Cas9 (Synthetic)Worm Expression, CRISPRCRISPR/Cas9-Targeted Mutagenesis in Caenorhabditis elegans. Genetics. 2013 Aug 26. Expresses C. elegans optimized Cas9::mEGFP from the eef-1A.1 (eft-3) promoter pBluescript SK+ Add to Cart
47945pMB63Cas9 (Synthetic)Worm Expression, CRISPRCRISPR/Cas9-Targeted Mutagenesis in Caenorhabditis elegans. Genetics. 2013 Aug 26. Expresses C. elegans optimized Cas9 from the eef-1A.1 (eft-3) promoter pBluescript SK+ Add to Cart
47946pMB66Cas9 (Synthetic)Worm Expression, CRISPRCRISPR/Cas9-Targeted Mutagenesis in Caenorhabditis elegans. Genetics. 2013 Aug 26. Expresses C. elegans optimized Cas9::mEGFP from the hsp-16.48 promoter pBluescript SK+ Add to Cart
47947pMB67Cas9 (Synthetic)Worm Expression, CRISPRCRISPR/Cas9-Targeted Mutagenesis in Caenorhabditis elegans. Genetics. 2013 Aug 26. Expresses C. elegans optimized Cas9 from the hsp-16.48 promoter pBluescript SK+ Add to Cart
73084pMB37BirAWorm Expression Boxem lab BirA and Avi-tag plasmids (unpublished) Expresses C. elegans optimized BirA under control of the ubiquitous rps-27 promoter pBluescript Add to Cart
73085pMB71BirAWorm Expression Boxem lab BirA and Avi-tag plasmids (unpublished) Expresses C. elegans optimized BirA under control of the intestinal elt-2 promoter pBluescript Add to Cart
73086pMB73BirAWorm Expression Boxem lab BirA and Avi-tag plasmids (unpublished) Expresses C. elegans optimized BirA under control of the seam and hypodermis wrt-2 promoter pBluescript Add to Cart
73087pMB41Avi-GFP tagWorm Expression Boxem lab BirA and Avi-tag plasmids (unpublished) N-terminal Avi-2xTEV-GFP vector, for fosmid recombineering pUC19 Add to Cart
73088pMB72GFP-Avi tagWorm Expression Boxem lab BirA and Avi-tag plasmids (unpublished) C-terminal GFP-2xTEV-Avi vector, for fosmid recombineering pUC19 Add to Cart
75026pJJR50guide RNA, flipped and extended version (Caenorhabditis elegans)CRISPR Boxem lab CRISPR plasmids (unpublished) U6 promoter driven flipped + extended sgRNA expression vector pBluescript Add to Cart
75027pJJR82EGFP^SEC^3xFlag (Caenorhabditis elegans)Worm Expression, CRISPR Boxem lab CRISPR plasmids (unpublished) EGFP^SEC^3xFlag vector with ccdB markers for cloning homology arms pDD282 Add to Cart
75028pJJR83mCherry^SEC^3xFlag (Caenorhabditis elegans)Worm Expression, CRISPR Boxem lab CRISPR plasmids (unpublished) mCherry^SEC^3xFlag vector with ccdB markers for cloning homology arms pDD282 Add to Cart
75029pJJR81tagBFP2^SEC^3xFlag (Caenorhabditis elegans)Worm Expression, CRISPR Boxem lab CRISPR plasmids (unpublished) tagBFP2^SEC^3xFlag vector with ccdB markers for cloning homology arms pDD282 Add to Cart