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pSico PGK puro
(Plasmid #11586)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 11586 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pSico PGK puro
  • Backbone size (bp) 7703
  • Modifications to backbone
    Puromycin is expressed from this plasmid as a selectable marker. Cre causes the puromycin gene to be recombined out of the construct, activating shRNA expression.
  • Vector type
    Mammalian Expression, Lentiviral, RNAi, Cre/Lox
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    None

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HpaI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer n/a
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

pSico PGK puro allows for conditional (Cre-Lox), stable expression of shRNAs for RNA interference in cells and transgenic mice. Addition of Cre TURNS ON shRNA expression.

The shRNA coding oligos have to be cloned into the HpaI and XhoI restriction sites. Oligo design information can be found on the author's map or on the Jacks Lab website http://web.mit.edu/ccr/labs/jacks/protocols/pSico.html

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSico PGK puro was a gift from Tyler Jacks (Addgene plasmid # 11586)
  • For your References section:

    Cre-lox-regulated conditional RNA interference from transgenes. Ventura A, Meissner A, Dillon CP, McManus M, Sharp PA, Van Parijs L, Jaenisch R, Jacks T. Proc Natl Acad Sci U S A. 2004 Jul 13. 101(28):10380-5. 10.1073/pnas.0403954101 PubMed 15240889