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Plasmid 12093: pSico PGK GFP
  • None

  • pSico PGK GFP
    (Search Vector Database)

  • Mammalian Expression, Lentiviral, RNAi, Cre/Lox

  • 7500

  • EGFP is expressed from this plasmid as a marker, but it is not a fusion protein. Cre causes EGFP to be recombined out of the construct, activating shRNA expression.

  • HpaI

  • No

  • XhoI

  • No

  • EGFP-C List of Sequencing Primers

  • Ampicillin

  • DH5alpha

  • 37

  • High Copy

  • View sequences (4)
  • Tyler Jacks

  • UBMTA
    Ancillary Agreement for Plasmids Containing FP Materials

Comments: 

pSico PGK GFP is similar to pSico, except the CMV promoter is replaced with the PGK promoter. pSico PGK GFP allows for conditional (Cre-Lox), stable expression of shRNAs for RNA interference in cells and transgenic mice. Addition of Cre TURNS ON shRNA expression.

The shRNA coding oligos have to be cloned into the HpaI and XhoI restriction sites. Oligo design information can be found on the author's map or on the Jacks Lab website http://web.mit.edu/jacks-lab/protocols_table.html

Addgene has sequenced a portion of this plasmid for verification. Full plasmid sequence is available only if provided by the depositing laboratory.

Article: Cre-lox-regulated conditional RNA interference from transgenes. Ventura et al (Proc Natl Acad Sci U S A. 2004 Jul 13. 101(28):10380-5. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 12093" in your Materials and Methods section.

Price: US $65

Available to academic and non-profits only