Plasmid 12093: pSico PGK GFP

• Gene/insert name: None
• Vector backbone: pSico PGK GFP
  (Search Vector Database)
• Vector type: Mammalian Expression, Lentiviral, RNAi, Cre/Lox
• Backbone size (bp): 7500
• Modifications to Backbone: EGFP is expressed from this plasmid as a marker, but it is not a fusion protein. Cre causes EGFP to be recombined out of the construct, activating shRNA expression.
• Cloning site 5': HpaI
• Site destroyed during cloning: No
• Cloning site 3': XhoI
• Site destroyed during cloning: No
• 5' sequencing primer: EGFP-C
• Bacterial resistance(s) Ampicillin
• Growth strain(s) DH5alpha
• Growth temperature (℃): 37
• High or low copy: High Copy
• Sequence: View sequences (1)
• Principal Investigator: Tyler Jacks
• Terms and Licenses: MTA

Comments: 

pSico PGK GFP is similar to pSico, except the CMV promoter is replaced with the PGK promoter. pSico PGK GFP allows for conditional (Cre-Lox), stable expression of shRNAs for RNA interference in cells and transgenic mice. Addition of Cre TURNS ON shRNA expression.

The shRNA coding oligos have to be cloned into the HpaI and XhoI restriction sites. Oligo design information can be found on the author's map or on the Jacks Lab website http://web.mit.edu/ccr/labs/jacks/protocols/pSi...

Article: Cre-lox-regulated conditional RNA interference from transgenes. Ventura et al (Proc Natl Acad Sci U S A. 2004 Jul 13. 101(28):10380-5. PubMed)