Addgene - pSicoR Plasmid Data

Plasmid 11579: pSicoR

Gene/insert name:
None
Vector backbone:
pSicoR
(Search Vector Database)
Vector type:
Mammalian Expression, Lentiviral, RNAi, Cre/Lox
Backbone size (bp):
7567
Modifications to Backbone:
EGFP is expressed from this plasmid as a marker, but it is not a fusion protein. EGFP and the shRNA oligo are flanked by LoxP sites. Cre causes both to be recombined out of the construct, turning off shRNA expression.
Cloning site 5':
HpaI
Site destroyed during cloning:
No
Cloning site 3':
XhoI
Site destroyed during cloning:
No
5' sequencing primer:
mU6-F (5'-ATATCCCTTGGAGAAAAGCCTT-3') List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (3)
Map:
View map
Supplemental document:
pSico shRNA cloning (application/pdf)
Principal Investigator:
Tyler Jacks
Terms and Licenses:
MTA

Comments:

pSicoR allows for conditional (Cre-Lox), stable expression of shRNAs for RNA interference in cells and transgenic mice. Addition of Cre TURNS OFF shRNA expression.

The shRNA coding oligos have to be cloned into the HpaI and XhoI restriction sites. Oligo design information can be found on the author's map for pSico and on the Jacks Lab website http://web.mit.edu/ccr/labs/jacks/protocols/pSi...

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Cre-lox-regulated conditional RNA interference from transgenes. Ventura et al (Proc Natl Acad Sci U S A. 2004 Jul 13. 101(28):10380-5. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 11579" in your Materials and Methods section.