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Plasmid 11578: pSico
  • None

  • pSico
    (Search Vector Database)

  • Mammalian Expression, Lentiviral, RNAi, Cre/Lox

  • 7566

  • EGFP is expressed from this plasmid as a marker, but it is not a fusion protein. Cre causes EGFP to be recombined out of the construct, activating shRNA expression.

  • HpaI

  • No

  • XhoI

  • No

  • EGFP-C List of Sequencing Primers

  • Ampicillin

  • DH5alpha

  • 37

  • High Copy

  • View sequences (4)
  • View map

  • pSico shRNA cloning (application/pdf)

  • Notes from Addgene (1)

  • Tyler Jacks

  • UBMTA

Comments: 

pSico allows for conditional (Cre-Lox), stable expression of shRNAs for RNA interference in cells and transgenic mice. Addition of Cre TURNS ON shRNA expression.

The shRNA coding oligos have to be cloned into the HpaI and XhoI restriction sites. Oligo design information can be found on the author's map or on http://web.mit.edu/jacks-lab/ (the Jacks Lab website)

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Cre-lox-regulated conditional RNA interference from transgenes. Ventura et al (Proc Natl Acad Sci U S A. 2004 Jul 13. 101(28):10380-5. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 11578" in your Materials and Methods section.

Price: US $65

Available to academic and non-profits only