Addgene - pSicoR PGK puro Plasmid Data

Plasmid 12084: pSicoR PGK puro

Gene/insert name:
None
Vector backbone:
pSicoR PGK puro
(Search Vector Database)
Vector type:
Mammalian Expression, Lentiviral, RNAi, Cre/Lox
Backbone size (bp):
7700
Modifications to Backbone:
Puromycin is expressed from this plasmid as a selectable marker. Puromycin and the shRNA oligo are flanked by LoxP sites. Cre causes both to be recombined out of the construct, turning off shRNA expression.
Cloning site 5':
HpaI
Site destroyed during cloning:
No
Cloning site 3':
XhoI
Site destroyed during cloning:
No
5' sequencing primer:
mU6-F List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
Puromycin
Sequence: View sequences (2)
Principal Investigator:
Tyler Jacks
Terms and Licenses:
MTA

Comments:

pSicoR PGK Puro allows for conditional (Cre-Lox), stable expression of shRNAs for RNA interference in cells and transgenic mice. Addition of Cre TURNS OFF shRNA expression. See pSicoR and pSico PGK Puro for more information.

The shRNA coding oligos have to be cloned into the HpaI and XhoI restriction sites. Oligo design information can be found on the author's map for pSico and on the Jacks Lab website http://web.mit.edu/ccr/labs/jacks/protocols/pSi...

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Cre-lox-regulated conditional RNA interference from transgenes. Ventura et al (Proc Natl Acad Sci U S A. 2004 Jul 13. 101(28):10380-5. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 12084" in your Materials and Methods section.

Price: $65.00

Available to academic and non-profits only

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This is commonly requested with

11586: pSico PGK puro