pSicoR PGK puro
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||12084||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Vector backbonepSicoR PGK puro
- Backbone size (bp) 7700
Modifications to backbonePuromycin is expressed from this plasmid as a selectable marker. Puromycin and the shRNA oligo are flanked by LoxP sites. Cre causes both to be recombined out of the construct, turning off shRNA expression.
Vector typeMammalian Expression, Lentiviral, RNAi, Cre/Lox
Growth in Bacteria
Copy numberHigh Copy
- Cloning method Restriction Enzyme
- 5′ cloning site HpaI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer mU6-F (Common Sequencing Primers)
pSicoR PGK Puro allows for conditional (Cre-Lox), stable expression of shRNAs for RNA interference in cells and transgenic mice. Addition of Cre TURNS OFF shRNA expression. See pSicoR and pSico PGK Puro for more information.
The shRNA coding oligos have to be cloned into the HpaI and XhoI restriction sites. Oligo design information can be found on the author's map for pSico and on the Jacks Lab website http://web.mit.edu/jacks-lab/protocols/pSico.html
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pSicoR PGK puro was a gift from Tyler Jacks (Addgene plasmid # 12084 ; http://n2t.net/addgene:12084 ; RRID:Addgene_12084)
For your References section:Cre-lox-regulated conditional RNA interference from transgenes. Ventura A, Meissner A, Dillon CP, McManus M, Sharp PA, Van Parijs L, Jaenisch R, Jacks T. Proc Natl Acad Sci U S A. 2004 Jul 13. 101(28):10380-5. 10.1073/pnas.0403954101 PubMed 15240889