Puromycin is expressed from this plasmid as a selectable marker. Puromycin and the shRNA oligo are flanked by LoxP sites. Cre causes both to be recombined out of the construct, turning off shRNA expression.
Please acknowledge the principal investigator and cite this article if you use
this plasmid in a publication. Also, please include the text "Addgene plasmid
12084" in your Materials and Methods section.