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David Sabatini Lab Plasmids


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Title Authors Publication
Genetic screens in human cells using the CRISPR-Cas9 system. Wang T, Wei JJ, Sabatini DM, Lander ES Science. 2014 Jan 3;343(6166):80-4. doi: 10.1126/science.1246981. Epub 2013 Dec 12.
mTORC1 phosphorylation sites encode their sensitivity to starvation and rapamycin. Kang SA, Pacold ME, Cervantes CL, Lim D, Lou HJ, Ottina K, Gray NS, Turk BE, Yaffe MB, Sabatini DM Science. 2013 Jul 26;341(6144):1236566. doi: 10.1126/science.1236566.
A Tumor suppressor complex with GAP activity for the Rag GTPases that signal amino acid sufficiency to mTORC1. Bar-Peled L, Chantranupong L, Cherniack AD, Chen WW, Ottina KA, Grabiner BC, Spear ED, Carter SL, Meyerson M, Sabatini DM Science. 2013 May 31;340(6136):1100-6. doi: 10.1126/science.1232044.
Ragulator Is a GEF for the Rag GTPases that Signal Amino Acid Levels to mTORC1. Bar-Peled L, Schweitzer LD, Zoncu R, Sabatini DM Cell. 2012 Sep 14;150(6):1196-208. doi: 10.1016/j.cell.2012.07.032.
A unifying model for mTORC1-mediated regulation of mRNA translation. Thoreen CC, Chantranupong L, Keys HR, Wang T, Gray NS, Sabatini DM Nature. 2012 May 2;485(7396):109-13. doi: 10.1038/nature11083.
mTORC1 senses lysosomal amino acids through an inside-out mechanism that requires the vacuolar H-ATPase. Zoncu R, Bar-Peled L, Efeyan A, Wang S, Sancak Y, Sabatini DM Science. 2011 Nov 4;334(6056):678-83.
mTOR Complex 1 Regulates Lipin 1 Localization to Control the SREBP Pathway. Peterson TR, Sengupta SS, Harris TE, Carmack AE, Kang SA, Balderas E, Guertin DA, Madden KL, Carpenter AE, Finck BN, Sabatini DM Cell. 2011 Aug 5;146(3):408-20.
The mTOR-regulated phosphoproteome reveals a mechanism of mTORC1-mediated inhibition of growth factor signaling. Hsu PP, Kang SA, Rameseder J, Zhang Y, Ottina KA, Lim D, Peterson TR, Choi Y, Gray NS, Yaffe MB, Marto JA, Sabatini DM Science. 2011 Jun 10;332(6035):1317-22.
Defective Regulation of Autophagy upon Leucine Deprivation Reveals a Targetable Liability of Human Melanoma Cells In Vitro and In Vivo. Sheen JH, Zoncu R, Kim D, Sabatini DM Cancer Cell. 2011 May 17. 19(5):613-28.
Ragulator-Rag complex targets mTORC1 to the lysosomal surface and is necessary for its activation by amino acids. Sancak Y, Bar-Peled L, Zoncu R, Markhard AL, Nada S, Sabatini DM Cell. 2010 Apr 16. 141(2):290-303.
DEPTOR Is an mTOR Inhibitor Frequently Overexpressed in Multiple Myeloma Cells and Required for Their Survival. Peterson TR, Laplante M, Thoreen CC, Sancak Y, Kang SA, Kuehl WM, Gray NS, Sabatini DM Cell. 2009 May 13. ():.
An ATP-competitive mammalian target of rapamycin inhibitor reveals rapamycin-resistant functions of mTORC1. Thoreen CC, Kang SA, Chang JW, Liu Q, Zhang J, Gao Y, Reichling LJ, Sim T, Sabatini DM, Gray NS J Biol Chem. 2009 Mar 20. 284(12):8023-32.
The Rag GTPases bind raptor and mediate amino acid signaling to mTORC1. Sancak Y, Peterson TR, Shaul YD, Lindquist RA, Thoreen CC, Bar-Peled L, Sabatini DM Science. 2008 Jun 13. 320(5882):1496-501.
PRAS40 is an insulin-regulated inhibitor of the mTORC1 protein kinase. Sancak Y, Thoreen CC, Peterson TR, Lindquist RA, Kang SA, Spooner E, Carr SA, Sabatini DM Mol Cell. 2007 Mar 23. 25(6):903-15.
mSin1 Is Necessary for Akt/PKB Phosphorylation, and Its Isoforms Define Three Distinct mTORC2s. Frias MA, Thoreen CC, Jaffe JD, Schroder W, Sculley T, Carr SA, Sabatini DM Curr Biol. 2006 Aug 15. ():.
Structure of S6K1 determines if raptor-mTOR or rictor-mTOR phosphorylates its hydrophobic motif site. Ali SM, Sabatini DM J Biol Chem 2005 Apr 4;.
Phosphorylation and regulation of Akt/PKB by the rictor-mTOR complex. Sarbassov DD, Guertin DA, Ali SM, Sabatini DM Science 2005 Feb 18;307(5712):1098-101.
Rictor, a novel binding partner of mTOR, defines a rapamycin-insensitive and raptor-independent pathway that regulates the cytoskeleton. Sarbassov DD, Ali SM, Kim DH, Guertin DA, Latek RR, Erdjument-Bromage H, Tempst P, Sabatini DM Curr Biol 2004 Jul 27;14(14):1296-302.
GbetaL, a positive regulator of the rapamycin-sensitive pathway required for the nutrient-sensitive interaction between raptor and mTOR. Kim DH, Sarbassov DD, Ali SM, Latek RR, Guntur KV, Erdjument-Bromage H, Tempst P, Sabatini DM Mol Cell 2003 Apr;11(4):895-904.
mTOR interacts with raptor to form a nutrient-sensitive complex that signals to the cell growth machinery. Kim DH, Sarbassov DD, Ali SM, King JE, Latek RR, Erdjument-Bromage H, Tempst P, Sabatini DM Cell 2002 Jul 26;110(2):163-75.
pLJM1-Flag (unpublished)
Unpublished plasmid (unpublished)
myc mTOR kinase dead (unpublished)
Plasmid Gene/Insert Vector Type Publication Hidden Extra Search Info  
Rictor_1 shRNA Rictor (Homo sapiens) Mammalian Expression, Lentiviral, RNAi Phosphorylation and regulation of Akt/PKB by the rictor-mTOR complex. Sarbassov et al Science 2005 Feb 18;307(5712):1098-101. pLKO.1 RICTOR AVO3, PIA, hAVO3 Add to Cart
Rictor_2 shRNA Rictor (Homo sapiens) Mammalian Expression, Lentiviral, RNAi Phosphorylation and regulation of Akt/PKB by the rictor-mTOR complex. Sarbassov et al Science 2005 Feb 18;307(5712):1098-101. pLKO.1 RICTOR AVO3, PIA, hAVO3 Add to Cart
mTOR_1 shRNA mTOR (Homo sapiens) Mammalian Expression, Lentiviral, RNAi Phosphorylation and regulation of Akt/PKB by the rictor-mTOR complex. Sarbassov et al Science 2005 Feb 18;307(5712):1098-101. pLKO.1 MTOR FRAP, FRAP1, FRAP2, RAFT1, RAPT1 Add to Cart
mTOR_2 shRNA mTOR (Homo sapiens) Mammalian Expression, Lentiviral, RNAi Phosphorylation and regulation of Akt/PKB by the rictor-mTOR complex. Sarbassov et al Science 2005 Feb 18;307(5712):1098-101. pLKO.1 MTOR FRAP, FRAP1, FRAP2, RAFT1, RAPT1 Add to Cart
Raptor_1 shRNA Raptor (Homo sapiens) Mammalian Expression, Lentiviral, RNAi Phosphorylation and regulation of Akt/PKB by the rictor-mTOR complex. Sarbassov et al Science 2005 Feb 18;307(5712):1098-101. pLKO.1 RPTOR KOG1, Mip1 Add to Cart
Raptor_2 shRNA Raptor (Homo sapiens) Mammalian Expression, Lentiviral, RNAi Phosphorylation and regulation of Akt/PKB by the rictor-mTOR complex. Sarbassov et al Science 2005 Feb 18;307(5712):1098-101. pLKO.1 RPTOR KOG1, Mip1 Add to Cart
myc-Raptor Raptor (Homo sapiens) Mammalian Expression Rictor, a novel binding partner of mTOR, defines a rapamycin-insensitive and raptor-independent pathway that regulates the cytoskeleton. Sarbassov et al Curr Biol 2004 Jul 27;14(14):1296-302. pRK-5 RPTOR KOG1, Mip1 Add to Cart
myc-Rictor Rictor (Homo sapiens) Mammalian Expression Rictor, a novel binding partner of mTOR, defines a rapamycin-insensitive and raptor-independent pathway that regulates the cytoskeleton. Sarbassov et al Curr Biol 2004 Jul 27;14(14):1296-302. pRK-5 RICTOR AVO3, PIA, hAVO3 Add to Cart
myc-mTOR mTOR (Rattus norvegicus) Mammalian Expression Rictor, a novel binding partner of mTOR, defines a rapamycin-insensitive and raptor-independent pathway that regulates the cytoskeleton. Sarbassov et al Curr Biol 2004 Jul 27;14(14):1296-302. pRK5 Mtor Frap1, RAFT1 Add to Cart
scramble shRNA scramble Mammalian Expression, Lentiviral, RNAi Phosphorylation and regulation of Akt/PKB by the rictor-mTOR complex. Sarbassov et al Science 2005 Feb 18;307(5712):1098-101. 3rd gen lentiviral negative control vector containing scrambled shRNA pLKO.1 Add to Cart
HA-GbL GbL (Homo sapiens) Mammalian Expression Rictor, a novel binding partner of mTOR, defines a rapamycin-insensitive and raptor-independent pathway that regulates the cytoskeleton. Sarbassov et al Curr Biol 2004 Jul 27;14(14):1296-302. pRK-5 MLST8 GBL, GbetaL, LST8, POP3, WAT1 Add to Cart
delta NT delta CT p70S6K1 MSCV puro delta NT delta CT p70 S6K1 (Rattus norvegicus) Mammalian Expression, Retroviral Structure of S6K1 determines if raptor-mTOR or rictor-mTOR phosphorylates its hydrophobic motif site. Ali et al J Biol Chem 2005 Apr 4;. mscv puro Rps6kb1 Add to Cart
F5A deltaCT S6K1 MSCV puro f5a delta ctp70 S6K1 (Rattus norvegicus) Mammalian Expression, Retroviral Structure of S6K1 determines if raptor-mTOR or rictor-mTOR phosphorylates its hydrophobic motif site. Ali et al J Biol Chem 2005 Apr 4;. mscv puro Rps6kb1 Add to Cart
deltaCT S6K1 MSCV puro delta ct p70 S6K1 (Rattus norvegicus) Mammalian Expression, Retroviral Structure of S6K1 determines if raptor-mTOR or rictor-mTOR phosphorylates its hydrophobic motif site. Ali et al J Biol Chem 2005 Apr 4;. mscv puro Rps6kb1 Add to Cart
HA GST p85 S6K1 pRK5 p85 s6k1 (Rattus norvegicus) Mammalian Expression Structure of S6K1 determines if raptor-mTOR or rictor-mTOR phosphorylates its hydrophobic motif site. Ali et al J Biol Chem 2005 Apr 4;. prk5 Rps6kb1 Add to Cart
HA GST F5A deltaCT S6K1 pRK5 f5a delta CT p85 s6k1 (Rattus norvegicus) Mammalian Expression Structure of S6K1 determines if raptor-mTOR or rictor-mTOR phosphorylates its hydrophobic motif site. Ali et al J Biol Chem 2005 Apr 4;. prk5 Rps6kb1 Add to Cart
HA GST deltaCT S6K1 pRK5 delta CT p85 s6k1 (Rattus norvegicus) Mammalian Expression Structure of S6K1 determines if raptor-mTOR or rictor-mTOR phosphorylates its hydrophobic motif site. Ali et al J Biol Chem 2005 Apr 4;. prk5 Rps6kb1 Add to Cart
HA Raptor mutant 1 Raptor (Homo sapiens) Mammalian Expression mTOR interacts with raptor to form a nutrient-sensitive complex that signals to the cell growth machinery. Kim et al Cell 2002 Jul 26;110(2):163-75. prk5 RPTOR KOG1, Mip1 Add to Cart
HA Raptor mutant 2 Raptor (Homo sapiens) Mammalian Expression mTOR interacts with raptor to form a nutrient-sensitive complex that signals to the cell growth machinery. Kim et al Cell 2002 Jul 26;110(2):163-75. prk5 RPTOR KOG1, Mip1 Add to Cart
HA Raptor mutant 3 Raptor (Homo sapiens) Mammalian Expression mTOR interacts with raptor to form a nutrient-sensitive complex that signals to the cell growth machinery. Kim et al Cell 2002 Jul 26;110(2):163-75. prk5 RPTOR KOG1, Mip1 Add to Cart
HA Raptor mutant 4 Raptor (Homo sapiens) Mammalian Expression mTOR interacts with raptor to form a nutrient-sensitive complex that signals to the cell growth machinery. Kim et al Cell 2002 Jul 26;110(2):163-75. prk5 RPTOR KOG1, Mip1 Add to Cart
HA Raptor mutant 7 Raptor (Homo sapiens) Mammalian Expression mTOR interacts with raptor to form a nutrient-sensitive complex that signals to the cell growth machinery. Kim et al Cell 2002 Jul 26;110(2):163-75. prk5 RPTOR KOG1, Mip1 Add to Cart
HA Raptor mutant 9 Raptor (Homo sapiens) Mammalian Expression mTOR interacts with raptor to form a nutrient-sensitive complex that signals to the cell growth machinery. Kim et al Cell 2002 Jul 26;110(2):163-75. prk5 RPTOR KOG1, Mip1 Add to Cart
myc GbetaL mutant 1 GbetaL (Homo sapiens) Mammalian Expression GbetaL, a positive regulator of the rapamycin-sensitive pathway required for the nutrient-sensitive interaction between raptor and mTOR. Kim et al Mol Cell 2003 Apr;11(4):895-904. prk5 MLST8 GBL, GbetaL, LST8, POP3, WAT1 Add to Cart
myc GbetaL mutant 2 GbetaL (Homo sapiens) Mammalian Expression GbetaL, a positive regulator of the rapamycin-sensitive pathway required for the nutrient-sensitive interaction between raptor and mTOR. Kim et al Mol Cell 2003 Apr;11(4):895-904. prk5 MLST8 GBL, GbetaL, LST8, POP3, WAT1 Add to Cart
myc GbetaL mutant 5 GbetaL (Homo sapiens) Mammalian Expression GbetaL, a positive regulator of the rapamycin-sensitive pathway required for the nutrient-sensitive interaction between raptor and mTOR. Kim et al Mol Cell 2003 Apr;11(4):895-904. prk5 MLST8 GBL, GbetaL, LST8, POP3, WAT1 Add to Cart
myc GbetaL mutant 6 GbetaL (Homo sapiens) Mammalian Expression GbetaL, a positive regulator of the rapamycin-sensitive pathway required for the nutrient-sensitive interaction between raptor and mTOR. Kim et al Mol Cell 2003 Apr;11(4):895-904. prk5 MLST8 GBL, GbetaL, LST8, POP3, WAT1 Add to Cart
myc GbetaL mutant 7 GbetaL (Homo sapiens) Mammalian Expression GbetaL, a positive regulator of the rapamycin-sensitive pathway required for the nutrient-sensitive interaction between raptor and mTOR. Kim et al Mol Cell 2003 Apr;11(4):895-904. prk5 MLST8 GBL, GbetaL, LST8, POP3, WAT1 Add to Cart
myc mTOR kinase dead mTOR (Rattus norvegicus) Mammalian Expression myc mTOR kinase dead (unpublished) prk5 Mtor Frap1, RAFT1 Add to Cart
HA Raptor Raptor (Homo sapiens) Mammalian Expression mTOR interacts with raptor to form a nutrient-sensitive complex that signals to the cell growth machinery. Kim et al Cell 2002 Jul 26;110(2):163-75. prk5 RPTOR KOG1, Mip1 Add to Cart
myc-Rictor corrected Rictor (Homo sapiens) Mammalian Expression Rictor, a novel binding partner of mTOR, defines a rapamycin-insensitive and raptor-independent pathway that regulates the cytoskeleton. Sarbassov et al Curr Biol 2004 Jul 27;14(14):1296-302. Mammalian expression of myc tagged human RICTOR pRK-5 RICTOR AVO3, PIA, hAVO3 Add to Cart
pMSCV-TAP Mammalian Expression, Retroviral mSin1 Is Necessary for Akt/PKB Phosphorylation, and Its Isoforms Define Three Distinct mTORC2s. Frias et al Curr Biol. 2006 Aug 15. ():. pMSCV-TAP Add to Cart
pMSCV-TAP mLST8 (aka: GbL) mLST8 (Homo sapiens) Mammalian Expression, Retroviral mSin1 Is Necessary for Akt/PKB Phosphorylation, and Its Isoforms Define Three Distinct mTORC2s. Frias et al Curr Biol. 2006 Aug 15. ():. pMSCV-TAP MLST8 GBL, GbetaL, LST8, POP3, WAT1 Add to Cart
pMSCV mSin1.1 myc mSin1.1 (Homo sapiens) Mammalian Expression, Retroviral mSin1 Is Necessary for Akt/PKB Phosphorylation, and Its Isoforms Define Three Distinct mTORC2s. Frias et al Curr Biol. 2006 Aug 15. ():. pMSCV MAPKAP1 RP11-269P11.1, JC310, MIP1, SIN1, SIN1b, SIN1g Add to Cart
pMSCV mSin1.2 myc mSin1.2 (Homo sapiens) Mammalian Expression, Retroviral mSin1 Is Necessary for Akt/PKB Phosphorylation, and Its Isoforms Define Three Distinct mTORC2s. Frias et al Curr Biol. 2006 Aug 15. ():. pMSCV MAPKAP1 RP11-269P11.1, JC310, MIP1, SIN1, SIN1b, SIN1g Add to Cart
pMSCV mSin1.4 myc mSin1.4 (Homo sapiens) Mammalian Expression, Retroviral mSin1 Is Necessary for Akt/PKB Phosphorylation, and Its Isoforms Define Three Distinct mTORC2s. Frias et al Curr Biol. 2006 Aug 15. ():. pMSCV MAPKAP1 RP11-269P11.1, JC310, MIP1, SIN1, SIN1b, SIN1g Add to Cart
pMSCV mSin1.5 myc mSin1.5 (Homo sapiens) Mammalian Expression, Retroviral mSin1 Is Necessary for Akt/PKB Phosphorylation, and Its Isoforms Define Three Distinct mTORC2s. Frias et al Curr Biol. 2006 Aug 15. ():. pMSCV MAPKAP1 RP11-269P11.1, JC310, MIP1, SIN1, SIN1b, SIN1g Add to Cart
pMSCV mLST8 myc (aka: GbL-myc) mLST8 (Homo sapiens) Mammalian Expression, Retroviral mSin1 Is Necessary for Akt/PKB Phosphorylation, and Its Isoforms Define Three Distinct mTORC2s. Frias et al Curr Biol. 2006 Aug 15. ():. pMSCV MLST8 GBL, GbetaL, LST8, POP3, WAT1 Add to Cart
pMSCV tubulin myc tubulin (Homo sapiens) Mammalian Expression, Retroviral mSin1 Is Necessary for Akt/PKB Phosphorylation, and Its Isoforms Define Three Distinct mTORC2s. Frias et al Curr Biol. 2006 Aug 15. ():. pMSCV TUBG1 GCP-1, TUBG, TUBGCP1 Add to Cart
pMSCV mSin1.1 HA mSin1.1 (Homo sapiens) Mammalian Expression, Retroviral mSin1 Is Necessary for Akt/PKB Phosphorylation, and Its Isoforms Define Three Distinct mTORC2s. Frias et al Curr Biol. 2006 Aug 15. ():. pMSCV MAPKAP1 RP11-269P11.1, JC310, MIP1, SIN1, SIN1b, SIN1g Add to Cart
pMSCV mSin1.2 HA mSin1.2 (Homo sapiens) Mammalian Expression, Retroviral mSin1 Is Necessary for Akt/PKB Phosphorylation, and Its Isoforms Define Three Distinct mTORC2s. Frias et al Curr Biol. 2006 Aug 15. ():. pMSCV MAPKAP1 RP11-269P11.1, JC310, MIP1, SIN1, SIN1b, SIN1g Add to Cart
pMSCV-TAP Raptor Raptor (Homo sapiens) Mammalian Expression, Retroviral mSin1 Is Necessary for Akt/PKB Phosphorylation, and Its Isoforms Define Three Distinct mTORC2s. Frias et al Curr Biol. 2006 Aug 15. ():. pMSCV RPTOR KOG1, Mip1 Add to Cart
mSin1 shRNA #1 mSin1 (Homo sapiens) Mammalian Expression, Lentiviral, RNAi mSin1 Is Necessary for Akt/PKB Phosphorylation, and Its Isoforms Define Three Distinct mTORC2s. Frias et al Curr Biol. 2006 Aug 15. ():. pLKO.1 MAPKAP1 RP11-269P11.1, JC310, MIP1, SIN1, SIN1b, SIN1g Add to Cart
mSin1 shRNA #2 mSin1 (Homo sapiens) Mammalian Expression, Lentiviral, RNAi mSin1 Is Necessary for Akt/PKB Phosphorylation, and Its Isoforms Define Three Distinct mTORC2s. Frias et al Curr Biol. 2006 Aug 15. ():. pLKO.1 MAPKAP1 RP11-269P11.1, JC310, MIP1, SIN1, SIN1b, SIN1g Add to Cart
pRK5 Flag PRAS40 PRAS40 (Homo sapiens) Mammalian Expression PRAS40 is an insulin-regulated inhibitor of the mTORC1 protein kinase. Sancak et al Mol Cell. 2007 Mar 23. 25(6):903-15. Flag-pRK5 AKT1S1 Lobe, PRAS40 Add to Cart
pRK5 HA GST Rheb1 Rheb1 (Homo sapiens) Mammalian Expression PRAS40 is an insulin-regulated inhibitor of the mTORC1 protein kinase. Sancak et al Mol Cell. 2007 Mar 23. 25(6):903-15. HA-GST-pRK5 RHEBP1 RHEB, RHEB1 Add to Cart
pRK5 HA GST rap2a rap2a (Homo sapiens) Mammalian Expression PRAS40 is an insulin-regulated inhibitor of the mTORC1 protein kinase. Sancak et al Mol Cell. 2007 Mar 23. 25(6):903-15. HA-GST-pRK5 RAP2A RP11-128N14.2, K-REV, KREV, RAP2, RbBP-30 Add to Cart
HA GST PreScission p70 S6K1 p70 S6K1 (Rattus norvegicus) Mammalian Expression PRAS40 is an insulin-regulated inhibitor of the mTORC1 protein kinase. Sancak et al Mol Cell. 2007 Mar 23. 25(6):903-15. HA-GST-pRK5 Rps6kb1 Add to Cart
pMT-puro Insect Expression ; Drosophila metallothionein gene promoter drives expression Unpublished plasmid (unpublished) Insect expression vector pMT-puro Add to Cart
pRK5-HA GST RagA wt Ras-related GTP binding A (Homo sapiens) Mammalian Expression The Rag GTPases bind raptor and mediate amino acid signaling to mTORC1. Sancak et al Science. 2008 Jun 13. 320(5882):1496-501. pRK5 RRAGA FIP-1, FIP1, RAGA Add to Cart
pRK5-HA GST RagA 21L Ras-related GTP binding A (Homo sapiens) Mammalian Expression The Rag GTPases bind raptor and mediate amino acid signaling to mTORC1. Sancak et al Science. 2008 Jun 13. 320(5882):1496-501. pRK5 RRAGA FIP-1, FIP1, RAGA Add to Cart
pRK5-HA GST RagA 66L Ras-related GTP binding A (Homo sapiens) Mammalian Expression The Rag GTPases bind raptor and mediate amino acid signaling to mTORC1. Sancak et al Science. 2008 Jun 13. 320(5882):1496-501. pRK5 RRAGA FIP-1, FIP1, RAGA Add to Cart
pRK5-HA GST RagB wt Ras-related GTP binding B (Homo sapiens) Mammalian Expression The Rag GTPases bind raptor and mediate amino acid signaling to mTORC1. Sancak et al Science. 2008 Jun 13. 320(5882):1496-501. pRK5 RRAGB RP11-465E19.1, RAGB, bA465E19.1 Add to Cart
pRK5-HA GST RagB 54L Ras-related GTP binding B (Homo sapiens) Mammalian Expression The Rag GTPases bind raptor and mediate amino acid signaling to mTORC1. Sancak et al Science. 2008 Jun 13. 320(5882):1496-501. pRK5 RRAGB RP11-465E19.1, RAGB, bA465E19.1 Add to Cart
pRK5-HA GST RagB 99L Ras-related GTP binding B (Homo sapiens) Mammalian Expression The Rag GTPases bind raptor and mediate amino acid signaling to mTORC1. Sancak et al Science. 2008 Jun 13. 320(5882):1496-501. pRK5 RRAGB RP11-465E19.1, RAGB, bA465E19.1 Add to Cart
pRK5-HA GST RagC wt Ras-related GTP binding C (Homo sapiens) Mammalian Expression The Rag GTPases bind raptor and mediate amino acid signaling to mTORC1. Sancak et al Science. 2008 Jun 13. 320(5882):1496-501. pRK5 RRAGC GTR2, RAGC, TIB929 Add to Cart
pRK5-HA GST RagC 75L Ras-related GTP binding C (Homo sapiens) Mammalian Expression The Rag GTPases bind raptor and mediate amino acid signaling to mTORC1. Sancak et al Science. 2008 Jun 13. 320(5882):1496-501. pRK5 RRAGC GTR2, RAGC, TIB929 Add to Cart
pRK5-HA GST RagC 120L Ras-related GTP binding C (Homo sapiens) Mammalian Expression The Rag GTPases bind raptor and mediate amino acid signaling to mTORC1. Sancak et al Science. 2008 Jun 13. 320(5882):1496-501. pRK5 RRAGC GTR2, RAGC, TIB929 Add to Cart
pRK5-HA GST RagD wt Ras-related GTP binding D (Homo sapiens) Mammalian Expression The Rag GTPases bind raptor and mediate amino acid signaling to mTORC1. Sancak et al Science. 2008 Jun 13. 320(5882):1496-501. pRK5 RRAGD RAGD, bA11D8.2.1 Add to Cart
pRK5-HA GST RagD 77L Ras-related GTP binding D (Homo sapiens) Mammalian Expression The Rag GTPases bind raptor and mediate amino acid signaling to mTORC1. Sancak et al Science. 2008 Jun 13. 320(5882):1496-501. pRK5 RRAGD RAGD, bA11D8.2.1 Add to Cart
pRK5-HA GST RagD 121L Ras-related GTP binding D (Homo sapiens) Mammalian Expression The Rag GTPases bind raptor and mediate amino acid signaling to mTORC1. Sancak et al Science. 2008 Jun 13. 320(5882):1496-501. pRK5 RRAGD RAGD, bA11D8.2.1 Add to Cart
pRK5-HA GST Rheb1 RHEB Ras homolog enriched in brain (Homo sapiens) Mammalian Expression The Rag GTPases bind raptor and mediate amino acid signaling to mTORC1. Sancak et al Science. 2008 Jun 13. 320(5882):1496-501. pRK5 RHEB RHEB2 Add to Cart
Flag pLJM1 Rap2A RAP2A, member of RAS oncogene family (Homo sapiens) Mammalian Expression, Lentiviral The Rag GTPases bind raptor and mediate amino acid signaling to mTORC1. Sancak et al Science. 2008 Jun 13. 320(5882):1496-501. pLJM1 RAP2A RP11-128N14.2, K-REV, KREV, RAP2, RbBP-30 Add to Cart
Flag pLJM1 Rheb1 RHEB Ras homolog enriched in brain (Homo sapiens) Mammalian Expression, Lentiviral The Rag GTPases bind raptor and mediate amino acid signaling to mTORC1. Sancak et al Science. 2008 Jun 13. 320(5882):1496-501. pLJM1 RHEB RHEB2 Add to Cart
Flag pLJM1 RagB wt Ras-related GTP binding B (Homo sapiens) Mammalian Expression, Lentiviral The Rag GTPases bind raptor and mediate amino acid signaling to mTORC1. Sancak et al Science. 2008 Jun 13. 320(5882):1496-501. pLJM1 RRAGB RP11-465E19.1, RAGB, bA465E19.1 Add to Cart
Flag pLJM1 RagB 54L Ras-related GTP binding B (Homo sapiens) Mammalian Expression, Lentiviral The Rag GTPases bind raptor and mediate amino acid signaling to mTORC1. Sancak et al Science. 2008 Jun 13. 320(5882):1496-501. pLJM1 RRAGB RP11-465E19.1, RAGB, bA465E19.1 Add to Cart
Flag pLJM1 RagB 99L Ras-related GTP binding B (Homo sapiens) Mammalian Expression, Lentiviral The Rag GTPases bind raptor and mediate amino acid signaling to mTORC1. Sancak et al Science. 2008 Jun 13. 320(5882):1496-501. pLJM1 RRAGB RP11-465E19.1, RAGB, bA465E19.1 Add to Cart
Flag pLJM1 RagD wt Ras-related GTP binding D (Homo sapiens) Mammalian Expression, Lentiviral The Rag GTPases bind raptor and mediate amino acid signaling to mTORC1. Sancak et al Science. 2008 Jun 13. 320(5882):1496-501. pLJM1 RRAGD RAGD, bA11D8.2.1 Add to Cart
Flag pLJM1 RagD 77L Ras-related GTP binding D (Homo sapiens) Mammalian Expression, Lentiviral The Rag GTPases bind raptor and mediate amino acid signaling to mTORC1. Sancak et al Science. 2008 Jun 13. 320(5882):1496-501. pLJM1 RRAGD RAGD, bA11D8.2.1 Add to Cart
Flag pLJM1 RagD 121L Ras-related GTP binding D (Homo sapiens) Mammalian Expression, Lentiviral The Rag GTPases bind raptor and mediate amino acid signaling to mTORC1. Sancak et al Science. 2008 Jun 13. 320(5882):1496-501. pLJM1 RRAGD RAGD, bA11D8.2.1 Add to Cart
pLJM1-EGFP none Mammalian Expression, Lentiviral The Rag GTPases bind raptor and mediate amino acid signaling to mTORC1. Sancak et al Science. 2008 Jun 13. 320(5882):1496-501. 3rd gen lentiviral vector for EGFP fusion; PGK driven puromycin pLJM1
  • Tag / Fusion Protein
    • EGFP
  • Add to Cart
    pRK5 FLAG human DEPTOR DEPTOR (Homo sapiens) Mammalian Expression DEPTOR Is an mTOR Inhibitor Frequently Overexpressed in Multiple Myeloma Cells and Required for Their Survival. Peterson et al Cell. 2009 May 13. ():. pRK5-FLAG DEPTOR DEP.6, DEPDC6 Add to Cart
    pLKO human shRNA 1 DEPTOR DEPTOR shRNA 1 (Homo sapiens) Mammalian Expression, Lentiviral, RNAi DEPTOR Is an mTOR Inhibitor Frequently Overexpressed in Multiple Myeloma Cells and Required for Their Survival. Peterson et al Cell. 2009 May 13. ():. pLKO.1 DEPTOR DEP.6, DEPDC6 Add to Cart
    pLKO human shRNA 2 DEPTOR DEPTOR shRNA 2 (Homo sapiens) Mammalian Expression, Lentiviral, RNAi DEPTOR Is an mTOR Inhibitor Frequently Overexpressed in Multiple Myeloma Cells and Required for Their Survival. Peterson et al Cell. 2009 May 13. ():. pLKO.1 DEPTOR DEP.6, DEPDC6 Add to Cart
    pLKO mouse shRNA 1 DEPTOR DEPTOR shRNA 1 (Mus musculus) Mammalian Expression, Lentiviral, RNAi DEPTOR Is an mTOR Inhibitor Frequently Overexpressed in Multiple Myeloma Cells and Required for Their Survival. Peterson et al Cell. 2009 May 13. ():. pLKO.1 Deptor 4731402B04Rik, 9130412E02Rik, D15Ertd336e, D15Ertd597e, Depdc6, Depdc6-001, Depdc6-002, R75183, mKIAA4200 Add to Cart
    pLKO mouse shRNA 2 DEPTOR DEPTOR shRNA 2 (Mus musculus) Mammalian Expression, Lentiviral, RNAi DEPTOR Is an mTOR Inhibitor Frequently Overexpressed in Multiple Myeloma Cells and Required for Their Survival. Peterson et al Cell. 2009 May 13. ():. pLKO.1 Deptor 4731402B04Rik, 9130412E02Rik, D15Ertd336e, D15Ertd597e, Depdc6, Depdc6-001, Depdc6-002, R75183, mKIAA4200 Add to Cart
    pLKO mouse shRNA 1 raptor raptor shRNA 1 (Mus musculus) Mammalian Expression, Lentiviral, RNAi An ATP-competitive mammalian target of rapamycin inhibitor reveals rapamycin-resistant functions of mTORC1. Thoreen et al J Biol Chem. 2009 Mar 20. 284(12):8023-32. pLKO.1 Rptor RP24-225K18.1, 4932417H02Rik, Rap, Raptor, mKIAA1303 Add to Cart
    pLKO mouse shRNA 2 raptor raptor shRNA 2 (Mus musculus) Mammalian Expression, Lentiviral, RNAi DEPTOR Is an mTOR Inhibitor Frequently Overexpressed in Multiple Myeloma Cells and Required for Their Survival. Peterson et al Cell. 2009 May 13. ():. pLKO.1 Add to Cart
    pLKO mouse shRNA 1 rictor rictor shRNA 1 (Mus musculus) Mammalian Expression, Lentiviral, RNAi An ATP-competitive mammalian target of rapamycin inhibitor reveals rapamycin-resistant functions of mTORC1. Thoreen et al J Biol Chem. 2009 Mar 20. 284(12):8023-32. pLKO.1 Rictor 4921505C17Rik, 6030405M08Rik, AVO3, AW492497, D530039E11Rik Add to Cart
    pRK5 FLAG DEPTOR (DEP domains) DEPTOR DEP domains (Homo sapiens) Mammalian Expression DEPTOR Is an mTOR Inhibitor Frequently Overexpressed in Multiple Myeloma Cells and Required for Their Survival. Peterson et al Cell. 2009 May 13. ():. pRK5-FLAG DEPTOR DEP.6, DEPDC6 Add to Cart
    pRK5 FLAG DEPTOR (PDZ domain) DEPTOR PDZ domain (Homo sapiens) Mammalian Expression DEPTOR Is an mTOR Inhibitor Frequently Overexpressed in Multiple Myeloma Cells and Required for Their Survival. Peterson et al Cell. 2009 May 13. ():. pRK5-FLAG DEPTOR DEP.6, DEPDC6 Add to Cart
    pRK5 FLAG DEPTOR (13×S/T->A) DEPTOR 13xA (Homo sapiens) Mammalian Expression DEPTOR Is an mTOR Inhibitor Frequently Overexpressed in Multiple Myeloma Cells and Required for Their Survival. Peterson et al Cell. 2009 May 13. ():. pRK5-FLAG DEPTOR DEP.6, DEPDC6 Add to Cart
    myc-mTOR (1-1482 aa) mTOR (1-1482) (Rattus norvegicus) Mammalian Expression GbetaL, a positive regulator of the rapamycin-sensitive pathway required for the nutrient-sensitive interaction between raptor and mTOR. Kim et al Mol Cell 2003 Apr;11(4):895-904. pRK5 Mtor Frap1, RAFT1 Add to Cart
    myc-mTOR (1271-2008 aa) mTOR (1271-2008) (Rattus norvegicus) Mammalian Expression GbetaL, a positive regulator of the rapamycin-sensitive pathway required for the nutrient-sensitive interaction between raptor and mTOR. Kim et al Mol Cell 2003 Apr;11(4):895-904. pRK5 Mtor Frap1, RAFT1 Add to Cart
    myc-mTOR (1750-2549 aa) mTOR (1750-2549) (Rattus norvegicus) Mammalian Expression GbetaL, a positive regulator of the rapamycin-sensitive pathway required for the nutrient-sensitive interaction between raptor and mTOR. Kim et al Mol Cell 2003 Apr;11(4):895-904. pRK5 Mtor Frap1, RAFT1 Add to Cart
    pLKO.1 Rheb1 shRNA #1 Rheb1 shRNA (Homo sapiens) Mammalian Expression, Lentiviral, RNAi The Rag GTPases bind raptor and mediate amino acid signaling to mTORC1. Sancak et al Science. 2008 Jun 13. 320(5882):1496-501. pLKO.1 puro RHEBP1 RHEB, RHEB1 Add to Cart
    pLKO.1 Rheb1 shRNA #2 Rheb1 shRNA (Homo sapiens) Mammalian Expression, Lentiviral, RNAi The Rag GTPases bind raptor and mediate amino acid signaling to mTORC1. Sancak et al Science. 2008 Jun 13. 320(5882):1496-501. pLKO.1 puro RHEBP1 RHEB, RHEB1 Add to Cart
    pLKO.1 RagB shRNA #1 RagB shRNA (Homo sapiens) Mammalian Expression, Lentiviral, RNAi The Rag GTPases bind raptor and mediate amino acid signaling to mTORC1. Sancak et al Science. 2008 Jun 13. 320(5882):1496-501. pLKO.1 puro RRAGB RP11-465E19.1, RAGB, bA465E19.1 Add to Cart
    pLKO.1 RagB shRNA #2 RagB shRNA (Homo sapiens) Mammalian Expression, Lentiviral, RNAi The Rag GTPases bind raptor and mediate amino acid signaling to mTORC1. Sancak et al Science. 2008 Jun 13. 320(5882):1496-501. pLKO.1 puro RRAGB RP11-465E19.1, RAGB, bA465E19.1 Add to Cart
    pLKO.1 RagC shRNA #1 RagC shRNA (Homo sapiens) Mammalian Expression, Lentiviral, RNAi The Rag GTPases bind raptor and mediate amino acid signaling to mTORC1. Sancak et al Science. 2008 Jun 13. 320(5882):1496-501. pLKO.1 puro RRAGC GTR2, RAGC, TIB929 Add to Cart
    pLKO.1 RagC shRNA #2 RagC shRNA (Homo sapiens) Mammalian Expression, Lentiviral, RNAi The Rag GTPases bind raptor and mediate amino acid signaling to mTORC1. Sancak et al Science. 2008 Jun 13. 320(5882):1496-501. pLKO.1 puro RRAGC GTR2, RAGC, TIB929 Add to Cart
    pLKO.1 p18 shRNA p18 shRNA (Homo sapiens) Mammalian Expression, Lentiviral, RNAi Ragulator-Rag complex targets mTORC1 to the lysosomal surface and is necessary for its activation by amino acids. Sancak et al Cell. 2010 Apr 16. 141(2):290-303. pLKO.1 puro LAMTOR1 PP7157, C11orf59, PDRO, Ragulator1, p18, p27RF-Rho Add to Cart
    pLKO.1 MP1 shRNA MP1 shRNA (Homo sapiens) Mammalian Expression, Lentiviral, RNAi Ragulator-Rag complex targets mTORC1 to the lysosomal surface and is necessary for its activation by amino acids. Sancak et al Cell. 2010 Apr 16. 141(2):290-303. pLKO.1 puro LAMTOR3 PRO2783, MAP2K1IP1, MAPBP, MAPKSP1, MP1, PRO0633, Ragulator3 Add to Cart
    pLJM1 Flag Raptor raptor (Homo sapiens) Mammalian Expression, Lentiviral Ragulator-Rag complex targets mTORC1 to the lysosomal surface and is necessary for its activation by amino acids. Sancak et al Cell. 2010 Apr 16. 141(2):290-303. pLJM1 RPTOR KOG1, Mip1 Add to Cart
    pLJM1 Flag Raptor Rheb15 raptor-Rheb15 (Homo sapiens) Mammalian Expression, Lentiviral Ragulator-Rag complex targets mTORC1 to the lysosomal surface and is necessary for its activation by amino acids. Sancak et al Cell. 2010 Apr 16. 141(2):290-303. pLJM1 RPTOR KOG1, Mip1 Add to Cart
    pLJM1 Flag Raptor HRas25 raptor-HRas25 (Homo sapiens) Mammalian Expression, Lentiviral Ragulator-Rag complex targets mTORC1 to the lysosomal surface and is necessary for its activation by amino acids. Sancak et al Cell. 2010 Apr 16. 141(2):290-303. pLJM1 RPTOR KOG1, Mip1 Add to Cart
    pLJM1 p18 mito p18 mito (Homo sapiens) Mammalian Expression, Lentiviral Ragulator-Rag complex targets mTORC1 to the lysosomal surface and is necessary for its activation by amino acids. Sancak et al Cell. 2010 Apr 16. 141(2):290-303. pLJM1 LAMTOR1 PP7157, C11orf59, PDRO, Ragulator1, p18, p27RF-Rho Add to Cart
    pLKO human shRNA 1 c-MAF c-MAF shRNA 1 (Homo sapiens) Mammalian Expression, Lentiviral, RNAi DEPTOR Is an mTOR Inhibitor Frequently Overexpressed in Multiple Myeloma Cells and Required for Their Survival. Peterson et al Cell. 2009 May 13. ():. pLKO.1 MAF CCA4, c-MAF Add to Cart
    pLKO human shRNA 2 c-MAF c-MAF shRNA 2 (Homo sapiens) Mammalian Expression, Lentiviral, RNAi DEPTOR Is an mTOR Inhibitor Frequently Overexpressed in Multiple Myeloma Cells and Required for Their Survival. Peterson et al Cell. 2009 May 13. ():. pLKO.1 MAF CCA4, c-MAF Add to Cart
    pLKO.1 RagA shRNA #1 RagA shRNA (Homo sapiens) Mammalian Expression, Lentiviral, RNAi The Rag GTPases bind raptor and mediate amino acid signaling to mTORC1. Sancak et al Science. 2008 Jun 13. 320(5882):1496-501. pLKO.1 puro RRAGA FIP-1, FIP1, RAGA Add to Cart
    pLKO.1 RagA shRNA #2 RagA shRNA (Homo sapiens) Mammalian Expression, Lentiviral, RNAi The Rag GTPases bind raptor and mediate amino acid signaling to mTORC1. Sancak et al Science. 2008 Jun 13. 320(5882):1496-501. pLKO.1 puro RRAGA FIP-1, FIP1, RAGA Add to Cart
    pLKO.1 RagD shRNA #1 RagD shRNA (Homo sapiens) Mammalian Expression, Lentiviral, RNAi The Rag GTPases bind raptor and mediate amino acid signaling to mTORC1. Sancak et al Science. 2008 Jun 13. 320(5882):1496-501. pLKO.1 puro RRAGD RAGD, bA11D8.2.1 Add to Cart
    pLKO.1 RagD shRNA #2 RagD shRNA (Homo sapiens) Mammalian Expression, Lentiviral, RNAi The Rag GTPases bind raptor and mediate amino acid signaling to mTORC1. Sancak et al Science. 2008 Jun 13. 320(5882):1496-501. pLKO.1 puro RRAGD RAGD, bA11D8.2.1 Add to Cart
    pLKO.1 GFP shRNA GFP shRNA Mammalian Expression, Lentiviral, RNAi The Rag GTPases bind raptor and mediate amino acid signaling to mTORC1. Sancak et al Science. 2008 Jun 13. 320(5882):1496-501. pLKO.1 puro Add to Cart
    pQCXI Puro DsRed-LC3-GFP DsRed-LC3-GFP Mammalian Expression, Retroviral Defective Regulation of Autophagy upon Leucine Deprivation Reveals a Targetable Liability of Human Melanoma Cells In Vitro and In Vivo. Sheen et al Cancer Cell. 2011 May 17. 19(5):613-28. pQCXIP Add to Cart
    pQCXI Neo DsRed-LC3-GFP DsRed-LC3-GFP Mammalian Expression, Retroviral Defective Regulation of Autophagy upon Leucine Deprivation Reveals a Targetable Liability of Human Melanoma Cells In Vitro and In Vivo. Sheen et al Cancer Cell. 2011 May 17. 19(5):613-28. pQCXIN Add to Cart
    pRK5 FLAG metap2 metap2 Mammalian Expression mTOR Complex 1 Regulates Lipin 1 Localization to Control the SREBP Pathway. Peterson et al Cell. 2011 Aug 5;146(3):408-20. pRK5 Metap2 4930584B20Rik, A930035J23Rik, AI047573, AL024412, AU014659, Amp2, MGC102452, Mnpep, p67, p67eIF2 Add to Cart
    pRK5 FLAG wildtype, catalytic active lipin 1 lipin1 (Mus musculus) Mammalian Expression mTOR Complex 1 Regulates Lipin 1 Localization to Control the SREBP Pathway. Peterson et al Cell. 2011 Aug 5;146(3):408-20. pRK5
  • Tag / Fusion Protein
    • FLAG (N terminal on backbone)
  • Lpin1 4631420P06, Kiaa0188, Lipin1, fld, mKIAA0188
    Add to Cart
    pRK5 FLAG wildtype, catalytic dead lipin 1 Lipin1 (Mus musculus) Mammalian Expression mTOR Complex 1 Regulates Lipin 1 Localization to Control the SREBP Pathway. Peterson et al Cell. 2011 Aug 5;146(3):408-20. pRK5 Lpin1 4631420P06, Kiaa0188, Lipin1, fld, mKIAA0188 Add to Cart
    pRK5 FLAG 17xS/T->A, catalytic active lipin 1 lipin1 (Mus musculus) Mammalian Expression mTOR Complex 1 Regulates Lipin 1 Localization to Control the SREBP Pathway. Peterson et al Cell. 2011 Aug 5;146(3):408-20. pRK5
  • Tag / Fusion Protein
    • FLAG (N terminal on backbone)
  • Lpin1 4631420P06, Kiaa0188, Lipin1, fld, mKIAA0188
    Add to Cart
    pRK5 FLAG 17xS/T->A, catalytic dead lipin 1 Lipin1 (Mus musculus) Mammalian Expression mTOR Complex 1 Regulates Lipin 1 Localization to Control the SREBP Pathway. Peterson et al Cell. 2011 Aug 5;146(3):408-20. pRK5
  • Tag / Fusion Protein
    • FLAG (N terminal on backbone)
  • Lpin1 4631420P06, Kiaa0188, Lipin1, fld, mKIAA0188
    Add to Cart
    pLKO-puro FLAG wildtype, catalytic active lipin 1 lipin1 (Mus musculus) Mammalian Expression, Lentiviral mTOR Complex 1 Regulates Lipin 1 Localization to Control the SREBP Pathway. Peterson et al Cell. 2011 Aug 5;146(3):408-20. pLKO.1-puro
  • Tag / Fusion Protein
    • FLAG (N terminal on backbone)
  • Lpin1 4631420P06, Kiaa0188, Lipin1, fld, mKIAA0188
    Add to Cart
    pLKO-puro FLAG SREBP1 SREBP1 (Mus musculus) Mammalian Expression, Lentiviral mTOR Complex 1 Regulates Lipin 1 Localization to Control the SREBP Pathway. Peterson et al Cell. 2011 Aug 5;146(3):408-20. pLKO.1-puro Srebf1 RP23-456O18.1, ADD-1, ADD1, D630008H06, SREBP-1, SREBP-1a, SREBP-1c, SREBP1, SREBP1c, bHLHd1 Add to Cart
    pLKO-puro FLAG SREBP2 SREBP2 (Mus musculus) Mammalian Expression, Lentiviral mTOR Complex 1 Regulates Lipin 1 Localization to Control the SREBP Pathway. Peterson et al Cell. 2011 Aug 5;146(3):408-20. pLKO.1-puro
  • Tag / Fusion Protein
    • FLAG (N terminal on backbone)
  • Srebf2 AI608257, SREBP-2, SREBP2, SREBP2gc, bHLHd2, lop13, nuc
    Add to Cart
    pLKO-puro human lipin 1-1 shRNA lipin1 shRNA (Homo sapiens) Mammalian Expression, Lentiviral, RNAi mTOR Complex 1 Regulates Lipin 1 Localization to Control the SREBP Pathway. Peterson et al Cell. 2011 Aug 5;146(3):408-20. pLKO.1-TRC Add to Cart
    pLJM60 deptor (Homo sapiens) Mammalian Expression, Lentiviral pLJM1-Flag (unpublished) pLJM1-Flag DEPTOR DEP.6, DEPDC6 Add to Cart
    LAMP1-mRFP-FLAG LAMP1 (Rattus norvegicus) Mammalian Expression, Lentiviral mTORC1 senses lysosomal amino acids through an inside-out mechanism that requires the vacuolar H-ATPase. Zoncu et al Science. 2011 Nov 4;334(6056):678-83. 3rd gen lentiviral vector expressing a fusion protein of Lamp1, mRFP, and a FLAG tag pLJM1 Add to Cart
    pRK5 Flag Grb10 wt long Grb10 (Homo sapiens) Mammalian Expression The mTOR-regulated phosphoproteome reveals a mechanism of mTORC1-mediated inhibition of growth factor signaling. Hsu et al Science. 2011 Jun 10;332(6035):1317-22. pRK5-FLAG GRB10 GRB-IR, Grb-10, IRBP, MEG1, RSS Add to Cart
    pRK5 Flag Grb10 5A short Grb10 5A (Homo sapiens) Mammalian Expression The mTOR-regulated phosphoproteome reveals a mechanism of mTORC1-mediated inhibition of growth factor signaling. Hsu et al Science. 2011 Jun 10;332(6035):1317-22. pRK5-FLAG
  • Tag / Fusion Protein
    • Flag (N terminal on backbone)
  • GRB10 GRB-IR, Grb-10, IRBP, MEG1, RSS
    Add to Cart
    pRK5 Flag Grb10 9A long Grb10 9A (Homo sapiens) Mammalian Expression The mTOR-regulated phosphoproteome reveals a mechanism of mTORC1-mediated inhibition of growth factor signaling. Hsu et al Science. 2011 Jun 10;332(6035):1317-22. pRK5-FLAG
  • Tag / Fusion Protein
    • Flag (N terminal on backbone)
  • GRB10 GRB-IR, Grb-10, IRBP, MEG1, RSS
    Add to Cart
    pIS1-Actb5UTR-renilla Actb 5'UTR / Renilla (Mus musculus) Mammalian Expression A unifying model for mTORC1-mediated regulation of mRNA translation. Thoreen et al Nature. 2012 May 2;485(7396):109-13. doi: 10.1038/nature11083. pIS1 Actb Actx, E430023M04Rik, beta-actin Add to Cart
    pIS1-Eef25UTR-renilla Eef2 5'UTR / Renilla (Mus musculus) Mammalian Expression, Luciferase A unifying model for mTORC1-mediated regulation of mRNA translation. Thoreen et al Nature. 2012 May 2;485(7396):109-13. doi: 10.1038/nature11083. pIS1 Eef2 Ef-2 Add to Cart
    pIS1-Eef25UTR-TOPmut-renilla Eef2_TOPm_5UTR/Renilla (Mus musculus) Mammalian Expression, Luciferase A unifying model for mTORC1-mediated regulation of mRNA translation. Thoreen et al Nature. 2012 May 2;485(7396):109-13. doi: 10.1038/nature11083. pIS1 Eef2 Ef-2 Add to Cart
    pIS1-Vim5UTR-renilla Vim 5'UTR / Renilla (Mus musculus) Mammalian Expression, Luciferase A unifying model for mTORC1-mediated regulation of mRNA translation. Thoreen et al Nature. 2012 May 2;485(7396):109-13. doi: 10.1038/nature11083. pIS1 Vim RP23-185P20.1 Add to Cart
    pIS1-Vim/Actb5UTR-renilla Vim promoter with Actb 5UTR/Renilla (Mus musculus) Mammalian Expression, Luciferase A unifying model for mTORC1-mediated regulation of mRNA translation. Thoreen et al Nature. 2012 May 2;485(7396):109-13. doi: 10.1038/nature11083. pIS1 Vim RP23-185P20.1 Actb Actx, E430023M04Rik, beta-actin Add to Cart
    pLJM60-eIF4E eIF4E (Rattus norvegicus) Mammalian Expression, Lentiviral A unifying model for mTORC1-mediated regulation of mRNA translation. Thoreen et al Nature. 2012 May 2;485(7396):109-13. doi: 10.1038/nature11083. pLJM60 Eif4e Add to Cart
    pCW57.1-4EBP1_4xAla 4EBP1 (Rattus norvegicus) Mammalian Expression, Lentiviral ; Doxycycline inducible A unifying model for mTORC1-mediated regulation of mRNA translation. Thoreen et al Nature. 2012 May 2;485(7396):109-13. doi: 10.1038/nature11083. pCW57.1 Eif4ebp1 PHAS-I Add to Cart
    pLJM60-Ssb Ssb (Homo sapiens) Mammalian Expression, Lentiviral A unifying model for mTORC1-mediated regulation of mRNA translation. Thoreen et al Nature. 2012 May 2;485(7396):109-13. doi: 10.1038/nature11083. pLJM60 SSB LARP3, La, La/SSB Add to Cart
    pLJM60-Auf1 AUF1 (Homo sapiens) Mammalian Expression, Lentiviral A unifying model for mTORC1-mediated regulation of mRNA translation. Thoreen et al Nature. 2012 May 2;485(7396):109-13. doi: 10.1038/nature11083. pLJM60
  • Tag / Fusion Protein
    • FLAG (N terminal on backbone)
  • AUF1 AUF1A HNRNPD AUF1, AUF1A, HNRPD, P37, hnRNPD0
    Add to Cart
    pLJM60-Tia1 Tia1 (Mus musculus) Mammalian Expression, Lentiviral A unifying model for mTORC1-mediated regulation of mRNA translation. Thoreen et al Nature. 2012 May 2;485(7396):109-13. doi: 10.1038/nature11083. pLJM60
  • Tag / Fusion Protein
    • FLAG (N terminal on backbone)
  • Tia1 2310050N03Rik, AI256674, TIA-1, mTIA-1
    Add to Cart
    pLJM60-Tiar Tiar (Mus musculus) Mammalian Expression, Lentiviral A unifying model for mTORC1-mediated regulation of mRNA translation. Thoreen et al Nature. 2012 May 2;485(7396):109-13. doi: 10.1038/nature11083. pLJM60
  • Tag / Fusion Protein
    • FLAG (N terminal on backbone)
  • Tial1 5330433G13Rik, AL033329, TIAR, mTIAR
    Add to Cart
    pLJM60-Ptb Ptb (Mus musculus) Mammalian Expression, Lentiviral A unifying model for mTORC1-mediated regulation of mRNA translation. Thoreen et al Nature. 2012 May 2;485(7396):109-13. doi: 10.1038/nature11083. pLJM60
  • Tag / Fusion Protein
    • FLAG (N terminal on backbone)
  • Add to Cart
    pLJM60-Cnbp Cnbp (Mus musculus) Mammalian Expression, Lentiviral A unifying model for mTORC1-mediated regulation of mRNA translation. Thoreen et al Nature. 2012 May 2;485(7396):109-13. doi: 10.1038/nature11083. pLJM60
  • Tag / Fusion Protein
    • FLAG (N terminal on backbone)
  • Cnbp AA408710, Cnbp1, Znf9
    Add to Cart
    pRK5-FLAG-RagB-D163N RagB D163N (Homo sapiens) Mammalian Expression Ragulator Is a GEF for the Rag GTPases that Signal Amino Acid Levels to mTORC1. Bar-Peled et al Cell. 2012 Sep 14;150(6):1196-208. doi: 10.1016/j.cell.2012.07.032. pRK5 RRAGB RP11-465E19.1, RAGB, bA465E19.1 Add to Cart
    pRK5-FLAG-RagC-D181N RagC D181N (Homo sapiens) Mammalian Expression Ragulator Is a GEF for the Rag GTPases that Signal Amino Acid Levels to mTORC1. Bar-Peled et al Cell. 2012 Sep 14;150(6):1196-208. doi: 10.1016/j.cell.2012.07.032. pRK5 RRAGC GTR2, RAGC, TIB929 Add to Cart
    pRK5-FLAG-HBXIP HBXIP (Homo sapiens) Mammalian Expression Ragulator Is a GEF for the Rag GTPases that Signal Amino Acid Levels to mTORC1. Bar-Peled et al Cell. 2012 Sep 14;150(6):1196-208. doi: 10.1016/j.cell.2012.07.032. pRK5 LAMTOR5 HBXIP, XIP Add to Cart
    pRK5-p18G2A-HA p18 G2A (Homo sapiens) Mammalian Expression Ragulator Is a GEF for the Rag GTPases that Signal Amino Acid Levels to mTORC1. Bar-Peled et al Cell. 2012 Sep 14;150(6):1196-208. doi: 10.1016/j.cell.2012.07.032. pRK5 LAMTOR1 PP7157, C11orf59, PDRO, Ragulator1, p18, p27RF-Rho Add to Cart
    pRK5-HA-HBXIP HBXIP (Homo sapiens) Mammalian Expression Ragulator Is a GEF for the Rag GTPases that Signal Amino Acid Levels to mTORC1. Bar-Peled et al Cell. 2012 Sep 14;150(6):1196-208. doi: 10.1016/j.cell.2012.07.032. pRK5
  • Tag / Fusion Protein
    • HA (N terminal on insert)
  • LAMTOR5 HBXIP, XIP
    Add to Cart
    pRK5-HA-mp1 mp1 (Homo sapiens) Mammalian Expression Ragulator Is a GEF for the Rag GTPases that Signal Amino Acid Levels to mTORC1. Bar-Peled et al Cell. 2012 Sep 14;150(6):1196-208. doi: 10.1016/j.cell.2012.07.032. pRK5 LAMTOR3 PRO2783, MAP2K1IP1, MAPBP, MAPKSP1, MP1, PRO0633, Ragulator3 Add to Cart
    pRK5-FLAG-p14 p14 (Homo sapiens) Mammalian Expression Ragulator Is a GEF for the Rag GTPases that Signal Amino Acid Levels to mTORC1. Bar-Peled et al Cell. 2012 Sep 14;150(6):1196-208. doi: 10.1016/j.cell.2012.07.032. pRK5 LAMTOR2 RP11-336K24.9, ENDAP, HSPC003, MAPBPIP, MAPKSP1AP, ROBLD3, Ragulator2, p14 Add to Cart
    pRK5-p18-FLAG p18 (Homo sapiens) Mammalian Expression Ragulator Is a GEF for the Rag GTPases that Signal Amino Acid Levels to mTORC1. Bar-Peled et al Cell. 2012 Sep 14;150(6):1196-208. doi: 10.1016/j.cell.2012.07.032. pRK5
  • Tag / Fusion Protein
    • Flag (C terminal on insert)
  • LAMTOR1 PP7157, C11orf59, PDRO, Ragulator1, p18, p27RF-Rho
    Add to Cart
    pRK5-FLAG-C7orf59 C7orf59 (Homo sapiens) Mammalian Expression Ragulator Is a GEF for the Rag GTPases that Signal Amino Acid Levels to mTORC1. Bar-Peled et al Cell. 2012 Sep 14;150(6):1196-208. doi: 10.1016/j.cell.2012.07.032. pRK5 LAMTOR4 C7orf59 Add to Cart
    N1-p18-EGFP p18 (Homo sapiens) Mammalian Expression Ragulator Is a GEF for the Rag GTPases that Signal Amino Acid Levels to mTORC1. Bar-Peled et al Cell. 2012 Sep 14;150(6):1196-208. doi: 10.1016/j.cell.2012.07.032. pEGFP-N1
  • Tag / Fusion Protein
    • EGFP (C terminal on backbone)
  • LAMTOR1 PP7157, C11orf59, PDRO, Ragulator1, p18, p27RF-Rho
    Add to Cart
    pRK5-HA-C7orf59 C7orf59 (Homo sapiens) Mammalian Expression Ragulator Is a GEF for the Rag GTPases that Signal Amino Acid Levels to mTORC1. Bar-Peled et al Cell. 2012 Sep 14;150(6):1196-208. doi: 10.1016/j.cell.2012.07.032. pRK5
  • Tag / Fusion Protein
    • HA (N terminal on insert)
  • LAMTOR4 C7orf59
    Add to Cart
    pRK5-p18-HA p18 (Homo sapiens) Mammalian Expression Ragulator Is a GEF for the Rag GTPases that Signal Amino Acid Levels to mTORC1. Bar-Peled et al Cell. 2012 Sep 14;150(6):1196-208. doi: 10.1016/j.cell.2012.07.032. pRK5
  • Tag / Fusion Protein
    • HA (C terminal on insert)
  • LAMTOR1 PP7157, C11orf59, PDRO, Ragulator1, p18, p27RF-Rho
    Add to Cart
    C terminal Flag Mios pRK5 Mios (Homo sapiens) Mammalian Expression A Tumor suppressor complex with GAP activity for the Rag GTPases that signal amino acid sufficiency to mTORC1. Bar-Peled et al Science. 2013 May 31;340(6136):1100-6. doi: 10.1126/science.1232044. pRK5 MIOS Add to Cart
    HA Depdc5 pRK5 Depdc5 (Homo sapiens) Mammalian Expression A Tumor suppressor complex with GAP activity for the Rag GTPases that signal amino acid sufficiency to mTORC1. Bar-Peled et al Science. 2013 May 31;340(6136):1100-6. doi: 10.1126/science.1232044. pRK5 DEPDC5 LL22NC03-113A11.1, DEP.5, FFEVF Add to Cart
    Cterminal HA WDR59 pRK5 WDR59 (Homo sapiens) Mammalian Expression A Tumor suppressor complex with GAP activity for the Rag GTPases that signal amino acid sufficiency to mTORC1. Bar-Peled et al Science. 2013 May 31;340(6136):1100-6. doi: 10.1126/science.1232044. pRK5 WDR59 FP977 Add to Cart
    Cterminal HA Mios pRK5 Mios (Homo sapiens) Mammalian Expression A Tumor suppressor complex with GAP activity for the Rag GTPases that signal amino acid sufficiency to mTORC1. Bar-Peled et al Science. 2013 May 31;340(6136):1100-6. doi: 10.1126/science.1232044. pRK5 MIOS Add to Cart
    HA Nprl3 pRK5 Nprl3 (Homo sapiens) Mammalian Expression A Tumor suppressor complex with GAP activity for the Rag GTPases that signal amino acid sufficiency to mTORC1. Bar-Peled et al Science. 2013 May 31;340(6136):1100-6. doi: 10.1126/science.1232044. pRK5 NPRL3 Z69666.1-003, C16orf35, CGTHBA, HS-40, MARE, NPR3, RMD11 Add to Cart
    HA Seh1L pRK5 Seh1 (Homo sapiens) Mammalian Expression A Tumor suppressor complex with GAP activity for the Rag GTPases that signal amino acid sufficiency to mTORC1. Bar-Peled et al Science. 2013 May 31;340(6136):1100-6. doi: 10.1126/science.1232044. pRK5 SEH1L SEC13L, SEH1A, SEH1B, Seh1 Add to Cart
    HA Sec13 pRK5 Sec13 (Homo sapiens) Mammalian Expression A Tumor suppressor complex with GAP activity for the Rag GTPases that signal amino acid sufficiency to mTORC1. Bar-Peled et al Science. 2013 May 31;340(6136):1100-6. doi: 10.1126/science.1232044. pRK5 SEC13 D3S1231E, SEC13L1, SEC13R, npp-20 Add to Cart
    Flag Nprl2 pRK5 Nprl2 (Homo sapiens) Mammalian Expression A Tumor suppressor complex with GAP activity for the Rag GTPases that signal amino acid sufficiency to mTORC1. Bar-Peled et al Science. 2013 May 31;340(6136):1100-6. doi: 10.1126/science.1232044. pRK5 NPRL2 NPR2, NPR2L, TUSC4 Add to Cart
    Flag WDR24 pRK5 WDR24 (Homo sapiens) Mammalian Expression A Tumor suppressor complex with GAP activity for the Rag GTPases that signal amino acid sufficiency to mTORC1. Bar-Peled et al Science. 2013 May 31;340(6136):1100-6. doi: 10.1126/science.1232044. pRK5 WDR24 LA16c-313D11.2, C16orf21, JFP7 Add to Cart
    HA WDR24 pRK5 WDR24 (Homo sapiens) Mammalian Expression A Tumor suppressor complex with GAP activity for the Rag GTPases that signal amino acid sufficiency to mTORC1. Bar-Peled et al Science. 2013 May 31;340(6136):1100-6. doi: 10.1126/science.1232044. pRK5 WDR24 LA16c-313D11.2, C16orf21, JFP7 Add to Cart
    Flag Depdc5 pLJM1 Depdc5 (Homo sapiens) Mammalian Expression, Lentiviral A Tumor suppressor complex with GAP activity for the Rag GTPases that signal amino acid sufficiency to mTORC1. Bar-Peled et al Science. 2013 May 31;340(6136):1100-6. doi: 10.1126/science.1232044. pLJM1 DEPDC5 LL22NC03-113A11.1, DEP.5, FFEVF Add to Cart
    Flag WDR24 pLJM1 WDR24 (Homo sapiens) Mammalian Expression, Lentiviral A Tumor suppressor complex with GAP activity for the Rag GTPases that signal amino acid sufficiency to mTORC1. Bar-Peled et al Science. 2013 May 31;340(6136):1100-6. doi: 10.1126/science.1232044. pLJM1 WDR24 LA16c-313D11.2, C16orf21, JFP7 Add to Cart
    Flag Nprl2 pLJM1 Nprl2 (Homo sapiens) Mammalian Expression, Lentiviral A Tumor suppressor complex with GAP activity for the Rag GTPases that signal amino acid sufficiency to mTORC1. Bar-Peled et al Science. 2013 May 31;340(6136):1100-6. doi: 10.1126/science.1232044. pLJM1 NPRL2 NPR2, NPR2L, TUSC4 Add to Cart
    Flag Depdc5 pRK5 Depdc5 (Homo sapiens) Mammalian Expression A Tumor suppressor complex with GAP activity for the Rag GTPases that signal amino acid sufficiency to mTORC1. Bar-Peled et al Science. 2013 May 31;340(6136):1100-6. doi: 10.1126/science.1232044. pRK5 DEPDC5 LL22NC03-113A11.1, DEP.5, FFEVF Add to Cart
    Flag Leucyl tRNA synthetase pRK5 leucyl tRNA synthetase (Homo sapiens) Mammalian Expression A Tumor suppressor complex with GAP activity for the Rag GTPases that signal amino acid sufficiency to mTORC1. Bar-Peled et al Science. 2013 May 31;340(6136):1100-6. doi: 10.1126/science.1232044. pRK5 LARS HSPC192, LARS1, LEURS, LEUS, LFIS, LRS, PIG44, RNTLS, hr025Cl Add to Cart
    eGFP Depdc5 pLJM1 Depdc5 (Homo sapiens) Mammalian Expression, Lentiviral A Tumor suppressor complex with GAP activity for the Rag GTPases that signal amino acid sufficiency to mTORC1. Bar-Peled et al Science. 2013 May 31;340(6136):1100-6. doi: 10.1126/science.1232044. pLJM1 DEPDC5 LL22NC03-113A11.1, DEP.5, FFEVF Add to Cart
    pLJM60 mUlk1 (wt) barcoded ULK1 (Mus musculus) Mammalian Expression, Lentiviral mTORC1 phosphorylation sites encode their sensitivity to starvation and rapamycin. Kang et al Science. 2013 Jul 26;341(6144):1236566. doi: 10.1126/science.1236566. expresses wt Ulk1 pLJM1 Ulk1 AU041434, Unc51.1, mKIAA0722 Add to Cart
    pLJM60-S6K1(T389S) barcoded S6K1 T389S (Rattus norvegicus) Mammalian Expression, Lentiviral mTORC1 phosphorylation sites encode their sensitivity to starvation and rapamycin. Kang et al Science. 2013 Jul 26;341(6144):1236566. doi: 10.1126/science.1236566. expresses mutant S6K1 pLJM1 Rps6kb1 Add to Cart
    pLJM60-S6K1(wt) barcoded S6K1 (Rattus norvegicus) Mammalian Expression, Lentiviral mTORC1 phosphorylation sites encode their sensitivity to starvation and rapamycin. Kang et al Science. 2013 Jul 26;341(6144):1236566. doi: 10.1126/science.1236566. expresses wt S6K1 pLJM1 Rps6kb1 Add to Cart
    pRK5 HA-GST-Presc-mAkt1 (wt) Akt1 (Mus musculus) Mammalian Expression mTORC1 phosphorylation sites encode their sensitivity to starvation and rapamycin. Kang et al Science. 2013 Jul 26;341(6144):1236566. doi: 10.1126/science.1236566. expresses wt Akt1 pRK5 Akt1 Akt, PKB, PKB/Akt, PKBalpha, Rac Add to Cart
    pRK5 HA-GST-Presc-mAkt1 (S473T) Akt1 (Mus musculus) Mammalian Expression mTORC1 phosphorylation sites encode their sensitivity to starvation and rapamycin. Kang et al Science. 2013 Jul 26;341(6144):1236566. doi: 10.1126/science.1236566. expresses mutant Akt1 pRK5 Akt1 Akt, PKB, PKB/Akt, PKBalpha, Rac Add to Cart
    pCW-Cas9 humanized S. pyogenes Cas9 Mammalian Expression, Lentiviral, CRISPR Genetic screens in human cells using the CRISPR-Cas9 system. Wang et al Science. 2014 Jan 3;343(6166):80-4. doi: 10.1126/science.1246981. Epub 2013 Dec 12. Inducible lentiviral expression of SpCas9 pCW57.1 Add to Cart
    pLX-sgRNA sgAAVS1 Mammalian Expression, Lentiviral, CRISPR Genetic screens in human cells using the CRISPR-Cas9 system. Wang et al Science. 2014 Jan 3;343(6166):80-4. doi: 10.1126/science.1246981. Epub 2013 Dec 12. Lentiviral expression of AAVS1-targeting sgRNA; insert can be replaced with custom sgRNA (see protocol) pLX304 Add to Cart
    Human Lentiviral sgRNA Library - Unknown Function sgRNA library (enriched for genes of unknown function) (Homo sapiens) Mammalian Expression, Lentiviral, CRISPR Genetic screens in human cells using the CRISPR-Cas9 system. Wang et al Science. 2014 Jan 3;343(6166):80-4. doi: 10.1126/science.1246981. Epub 2013 Dec 12. Sub-pool of Sabatini/Lander sgRNA library enriched for gene targets of unknown function. pLX-sgRNA Add to Cart
    Human Lentiviral sgRNA Library - Kinases sgRNA library (enriched for kinase targets) (Homo sapiens) Mammalian Expression, Lentiviral, CRISPR Genetic screens in human cells using the CRISPR-Cas9 system. Wang et al Science. 2014 Jan 3;343(6166):80-4. doi: 10.1126/science.1246981. Epub 2013 Dec 12. Sub-pool of Sabatini/Lander sgRNA library enriched for kinase targets. pLX-sgRNA Add to Cart
    Human Lentiviral sgRNA Library - Ribosomal Proteins sgRNA library (enriched for ribosomal targets) (Homo sapiens) Mammalian Expression, Lentiviral, CRISPR Genetic screens in human cells using the CRISPR-Cas9 system. Wang et al Science. 2014 Jan 3;343(6166):80-4. doi: 10.1126/science.1246981. Epub 2013 Dec 12. Sub-pool of Sabatini/Lander sgRNA library enriched for ribosomal gene targets. pLX-sgRNA Add to Cart
    Human Lentiviral sgRNA Library - Cell Cycle sgRNA library (enriched for cell cycle targets) (Homo sapiens) Mammalian Expression, Lentiviral, CRISPR Genetic screens in human cells using the CRISPR-Cas9 system. Wang et al Science. 2014 Jan 3;343(6166):80-4. doi: 10.1126/science.1246981. Epub 2013 Dec 12. Sub-pool of Sabatini/Lander sgRNA library enriched for cell cycle-related targets. pLX-sgRNA Add to Cart
    Human Lentiviral sgRNA Library - Nuclear sgRNA library (enriched for nuclear targets) (Homo sapiens) Mammalian Expression, Lentiviral, CRISPR Genetic screens in human cells using the CRISPR-Cas9 system. Wang et al Science. 2014 Jan 3;343(6166):80-4. doi: 10.1126/science.1246981. Epub 2013 Dec 12. Sub-pool of Sabatini/Lander sgRNA library enriched for nuclear targets. pLX-sgRNA Add to Cart
    Human Lentiviral sgRNA Library - Control sgRNA library (enriched for control targets) (Homo sapiens) Mammalian Expression, Lentiviral, CRISPR Genetic screens in human cells using the CRISPR-Cas9 system. Wang et al Science. 2014 Jan 3;343(6166):80-4. doi: 10.1126/science.1246981. Epub 2013 Dec 12. Sub-pool of Sabatini/Lander sgRNA library enriched for control targets. pLX-sgRNA Add to Cart