Addgene - pLL3.7 Plasmid Data

Gene/insert name:
None
Vector backbone:
pLL3.7
(Search Vector Database)
Vector type:
Mammalian Expression, Lentiviral, RNAi, Cre/Lox
Backbone size w/o insert:
7650
Cloning site 5':
HpaI
Site destroyed during cloning:
No
Cloning site 3':
XhoI
Site destroyed during cloning:
No
5' sequencing primer:
U6 (5'-cagtgcaggggaaagaatagtagac-3') List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
30
Growth instructions:
Although this plasmid grows in standard bacteria, to reduce the chance of recombination we recommend that you grow the plasmid at 30oC in Stbl3 cells from Invitrogen.
High or low copy:
High Copy
Selectable markers, other:
GFP
Sequence: View sequences (4)
Map:
View map
Supplemental document:
pLL3.7 shRNA cloning (application/pdf)
Principal Investigator:
Luk Parijs
Terms and Licenses:
MTA

Comments:

Lentiviral vector that expresses shRNA under the mouse U6 promoter. A CMV-EGFP reporter cassette is included in the vector to monitor expression.

For protocols regarding the use of pLL3.7, visit: http://web.mit.edu/jacks-lab/protocols/rnaireso...

Note: A single base pair deletion at position 11 of the U6 promoter in this plasmid does not impair the efficacy of this reagent.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: A lentivirus-based system to functionally silence genes in primary mammalian cells, stem cells and transgenic mice by RNA interference. Rubinson et al (Nat Genet. 2003 Mar . 33(3):401-6. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 11795" in your Materials and Methods section.