Addgene - pLB Plasmid Data

Plasmid 11619: pLB

Gene/insert name:
None
Fusion protein or tag:
GFP
Terminal:
C terminal on backbone
Vector backbone:
pLL3.7
(Search Vector Database)
Backbone manufacturer:
N/A
Vector type:
Mammalian Expression, Lentiviral, RNAi, Cre/Lox
Backbone size (bp):
8500
Cloning site 5':
HpaI
Site destroyed during cloning:
No
Cloning site 3':
XhoI
Site destroyed during cloning:
No
5' sequencing primer:
mU6-F List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (2)
Map:
View map
Principal Investigator:
Stephan Kissler
Terms and Licenses:
MTA

Comments:

pLB is a modification of pLL3.7. Two genetic elements known to prevent epigenetic silencing were added. A fragment of one antirepressor element (#40) was cloned upstream of the U6 promoter and a scaffold-attached region (SAR) was cloned downstream of GFP.

Please see author's map for more detailed information.

Note: A single base pair deletion at position 11 of the U6 promoter in this plasmid does not impair the efficacy of this reagent.
There is also a base pair insertion upstream of the promoter. The depositing lab has no indication that it functionally impairs pLB.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: In vivo RNA interference demonstrates a role for Nramp1 in modifying susceptibility to type 1 diabetes. Kissler et al (Nat Genet. 2006 Apr . 38(4):479-83. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 11619" in your Materials and Methods section.