Addgene - Tet-pLKO-puro Plasmid Data

Plasmid 21915: Tet-pLKO-puro

Gene/insert name:
None
Vector backbone:
Tet-pLKO-puro
(Search Vector Database)
Vector type:
Lentiviral
Backbone size (bp):
10633
Cloning site 5':
AgeI
Site destroyed during cloning:
No
Cloning site 3':
EcoRI
Site destroyed during cloning:
No
5' sequencing primer:
GGCAGGGATATTCACCATTATCGTTTCAGA List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
Stbl3
Growth temperature (℃):
37
Growth instructions:
recommend rec-deficient E.coli (Stbl3, SURE) at 37C
High or low copy:
Low Copy
Selectable markers:
Puromycin
Sequence: View sequences (3)
Supplemental document:
Tet-pLKO Manual (application/pdf)
Supplemental document:
Addgene's pLKO.1 protocol
Supplemental document:
Notes from Addgene (3)
Principal Investigator:
Dmitri Wiederschain
Terms and Licenses:
MTA

Comments:

This plasmid was called pLKO-Tet-On in the original publication. The name was changed to clarify that this plasmid does not contain and is not related to the trademarked Tet-On(R) system sold by Clontech. No other changes were made. Clontech does not support or recommend this product.

Please look at the manual associated with this plasmid.

For additional information please review the following reference -
Wee, S., Wiederschain, D., Maira, S.-M., Loo, A., Miller, C., deBeaumont, R., Stegmeier, F., Yao, Y.-M., and Lengauer, C. PTEN-deficient cancers depend on PIK3CB, Proc Natl Acad Sci U S A. 105: 13057-62, 2008.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Single-vector inducible lentiviral RNAi system for oncology target validation. Wiederschain et al (Cell Cycle. 2009 Feb 1. 8(3):498-504. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 21915" in your Materials and Methods section.