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pLKO.3 Thy1.1
(Plasmid #14749)

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Available to Academic and Nonprofits Only

Backbone

  • Vector backbone
    pLKO.3 Thy1.1
  • Backbone size (bp) 8000
  • Vector type
    Mammalian Expression, Lentiviral, RNAi
  • Selectable markers
    Thy1.1

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    High Copy

Sequence Information

Full plasmid sequence is available only if provided by the depositing laboratory.

Gene/Insert

  • Gene/Insert name
    None

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site PacI (not destroyed)
  • 5′ sequencing primer LKO.1 5'
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Derived from the pLKO.1 plasmid from The RNAi Consortium (TRC)
  • Terms and Licenses
  • Reference

Depositor Comments

A variant of pLKO.1 containing an Thy1.1 marker rather than puromycin.

pLKO.3 Thy 1.1 was cloned by excising PAC-GFP from pLKO.3pgw and replacing it with Thy1.1 that was PCR'd from another vector. Cloned into the BamHI and EcoRV sites of pLKO.3pgw.

See http://citdh.org/Lentivectors.html

for more information on pLKO.3pgw.

How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLKO.3 Thy1.1 was a gift from Christophe Benoist & Diane Mathis (Addgene plasmid # 14749)