1
Plasmid 14749: pLKO.3 Thy1.1
  • None

  • pLKO.3 Thy1.1
    (Search Vector Database)

  • Mammalian Expression, Lentiviral, RNAi

  • 8000

  • EcoRI

  • No

  • PacI

  • No

  • LKO.1 5' List of Sequencing Primers

  • Ampicillin

  • Stbl3

  • 37

  • High Copy

  • Thy1.1

  • Derived from the pLKO.1 plasmid from The RNAi Consortium (TRC)

  • View sequences (1)
  • Addgene's pLKO.1 protocol

  • Christophe Benoist

  • Diane Mathis

  • UBMTA

Comments: 

A variant of pLKO.1 containing an Thy1.1 marker rather than puromycin.

pLKO.3 Thy 1.1 was cloned by excising PAC-GFP from pLKO.3pgw and replacing it with Thy1.1 that was PCR'd from another vector. Cloned into the BamHI and EcoRV sites of pLKO.3pgw.

See http://citdh.org/Lentivectors.html

for more information on pLKO.3pgw.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 14749" in your Materials and Methods section.