1
Plasmid 14749: pLKO.3 Thy1.1
  • None

  • pLKO.3 Thy1.1
    (Search Vector Database)

  • Mammalian Expression, Lentiviral, RNAi

  • 8000

  • EcoRI

  • No

  • PacI

  • No

  • LKO.1 5' List of Sequencing Primers

  • Ampicillin

  • Stbl3

  • 37

  • High Copy

  • Thy1.1

  • Derived from the pLKO.1 plasmid from The RNAi Consortium (TRC)

  • View sequences (1)
  • Addgene's pLKO.1 protocol

  • Christophe Benoist

  • Diane Mathis

  • UBMTA

Comments: 

A variant of pLKO.1 containing an Thy1.1 marker rather than puromycin.

pLKO.3 Thy 1.1 was cloned by excising PAC-GFP from pLKO.3pgw and replacing it with Thy1.1 that was PCR'd from another vector. Cloned into the BamHI and EcoRV sites of pLKO.3pgw.

See http://citdh.org/Lentivectors.html

for more information on pLKO.3pgw.

Addgene has sequenced a portion of this plasmid for verification. Full plasmid sequence is available only if provided by the depositing laboratory.

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 14749" in your Materials and Methods section.

Price: US $65

Available to academic and non-profits only