Vector backbonepLKO.3 Thy1.1
- Backbone size (bp) 8000
Vector typeMammalian Expression, Lentiviral, RNAi
Growth in Bacteria
Copy numberHigh Copy
Full plasmid sequence is available only if provided by the depositing laboratory.
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site PacI (not destroyed)
- 5′ sequencing primer LKO.1 5' (Common Sequencing Primers)
A variant of pLKO.1 containing an Thy1.1 marker rather than puromycin.
pLKO.3 Thy 1.1 was cloned by excising PAC-GFP from pLKO.3pgw and replacing it with Thy1.1 that was PCR'd from another vector. Cloned into the BamHI and EcoRV sites of pLKO.3pgw.
for more information on pLKO.3pgw.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pLKO.3 Thy1.1 was a gift from Christophe Benoist & Diane Mathis (Addgene plasmid # 14749)
Map generated by Addgene from plasmid data.