Addgene - pLKO.3 Thy1.1 Plasmid Data

Plasmid 14749: pLKO.3 Thy1.1

Gene/insert name:
None
Vector backbone:
pLKO.3 Thy1.1
(Search Vector Database)
Vector type:
Mammalian Expression, Lentiviral, RNAi
Backbone size (bp):
8000
Cloning site 5':
EcoRI
Site destroyed during cloning:
No
Cloning site 3':
PacI
Site destroyed during cloning:
No
5' sequencing primer:
LKO.1 5' List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
Stbl3
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers, other:
Thy1.1
Person or lab that originally
cloned the gene/insert:
Derived from the pLKO.1 plasmid from The RNAi Consortium (TRC)
Sequence: View sequences (1)
Supplemental document:
Addgene's pLKO.1 protocol
Principal Investigator:
Christophe Benoist
Principal Investigator:
Diane Mathis
Terms and Licenses:
MTA

Comments:

A variant of pLKO.1 containing an Thy1.1 marker rather than puromycin.

pLKO.3 Thy 1.1 was cloned by excising PAC-GFP from pLKO.3pgw and replacing it with Thy1.1 that was PCR'd from another vector. Cloned into the BamHI and EcoRV sites of pLKO.3pgw.

See http://citdh.org/Lentivectors.html

for more information on pLKO.3pgw.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 14749" in your Materials and Methods section.