Addgene - pLKO.3G Plasmid Data

Gene/insert name:
None
Vector backbone:
pLKO.3G
(Search Vector Database)
Vector type:
Mammalian Expression, Lentiviral, RNAi
Backbone size w/o insert:
8174
Cloning site 5':
EcoRI
Site destroyed during cloning:
No
Cloning site 3':
PacI
Site destroyed during cloning:
No
5' sequencing primer:
LKO.1 5' List of Sequencing Primers
Bacterial resistance:
Ampicillin
Growth strain:
Stbl3
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers, other:
eGFP
Person or lab that originally
cloned the gene/insert:
Derived from the pLKO.1 plasmid from The RNAi Consortium (TRC)
Sequence: View sequences (2)
Map:
View map
Supplemental document:
Addgene's pLKO.1 protocol
Principal Investigator:
Christophe Benoist
Principal Investigator:
Diane Mathis
Terms and Licenses:
MTA

Comments:

A variant of pLKO.1 containing an eGFP marker rather than puromycin. Please note that the AgeI site is NOT unique for this vector, so shRNAs should be designed for cloning with EcoRI and PacI instead. Forward oligo: 5'-AATT—21bp sense—CTCGAG—21bp antisense—TTTTTTTAT-3'. Reverse oligo: 5'-AAAAAAA—21bp sense—CTCGAG—21bp antisense-3'.

pLKO.3G was cloned by excising PAC-GFP from pLKO.3pgw and replacing it with eGFP from another vector using the enzymes BamHI and BsRGI.

See http://citdh.org/Lentivectors.html

for more information.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 14748" in your Materials and Methods section.