1
Plasmid 14748: pLKO.3G
  • None

  • pLKO.3G
    (Search Vector Database)

  • Mammalian Expression, Lentiviral, RNAi

  • 8174

  • EcoRI

  • No

  • PacI

  • No

  • LKO.1 5' List of Sequencing Primers

  • Ampicillin

  • Stbl3

  • 37

  • High Copy

  • eGFP

  • Derived from the pLKO.1 plasmid from The RNAi Consortium (TRC)

  • View sequences (3)
  • View map

  • Addgene's pLKO.1 protocol

  • Notes from Addgene (1)

  • Christophe Benoist

  • Diane Mathis

  • UBMTA

Comments: 

A variant of pLKO.1 containing an eGFP marker rather than puromycin. Please note that the AgeI site is NOT unique for this vector, so shRNAs should be designed for cloning with EcoRI and PacI instead. Forward oligo: 5'-AATT—21bp sense—CTCGAG—21bp antisense—TTTTTTTAT-3'. Reverse oligo: 5'-AAAAAAA—21bp sense—CTCGAG—21bp antisense-3'.

pLKO.3G was cloned by excising PAC-GFP from pLKO.3pgw and replacing it with eGFP from another vector using the enzymes BamHI and BsRGI.

See http://citdh.org/Lentivectors.html

for more information.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 14748" in your Materials and Methods section.