Purpose(Empty Backbone) Tetracycline inducible 3rd generation lentiviral vector
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||39481||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
|Cloning Grade DNA||39481-DNA.cg||2 µg of cloning grade DNA in Tris buffer||1||$95|
This material is available to academics and nonprofits only.
Modifications to backboneRemoved the Gateway elements between the 2 EcoRV sites and replaced the Zeocin resistance gene with a puromycin resistance gene
Vector typeMammalian Expression, Lentiviral
- Promoter CMV
/ Fusion Protein
- V5 and 6XHis (C terminal on backbone)
Growth in Bacteria
Growth instructionsSee Depositor Comments below.
Copy numberHigh Copy
- Cloning method Restriction Enzyme
- 5′ sequencing primer CMV-fwd
- 3′ sequencing primer V5-rev and BGH-Rev (Common Sequencing Primers)
Please note this plasmid appears to be unstable during growth in bacteria. We recommend screening 3-5 smaller colonies after streaking from the original stab. It may be necessary to cut the plasmid with a single-cutting enzyme, gel purify the high molecular weight band, and religate to restore the plasmid.
Information for Cloning Grade DNA (Catalog # 39481-DNA.cg) ( Back to top )
Cloning grade DNA is suitable for use in PCR, cloning reactions, or transformation into E. coli. The purity and amount is not suitable for direct transfections.
- Amount 2 µg
- Guaranteed Concentration 100 ng/µl +/- 5 ng/µl
- Pricing $95 USD
- Storage DNA can be stored at 4℃ (short term) or -20℃ (long term).
Terms and Licenses
- Not Available to Industry
Addgene has verified this plasmid using Next Generation Sequencing. Results are available here
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pLenti-puro was a gift from Ie-Ming Shih (Addgene plasmid # 39481 ; http://n2t.net/addgene:39481 ; RRID:Addgene_39481)
For your References section:ARID1A, a factor that promotes formation of SWI/SNF-mediated chromatin remodeling, is a tumor suppressor in gynecologic cancers. Guan B, Wang TL, Shih IeM. Cancer Res. 2011 Nov 1;71(21):6718-27. Epub 2011 Sep 7. 10.1158/0008-5472.CAN-11-1562 PubMed 21900401