Addgene - pLionII Plasmid Data

Plasmid 1730: pLionII

Gene/insert name:
None
Vector backbone:
pLionII
(Search Vector Database)
Backbone manufacturer:
Nolan Lab
Vector type:
Lentiviral
Backbone size (bp):
7002
5' sequencing primer:
n/a List of Sequencing Primers
3' sequencing primer:
M13 reverse primer
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
Unknown
Sequence: View sequences (2)
Map:
View map
Supplemental document:
Nolan-felix (application/pdf)
Principal Investigator:
Garry Nolan
Terms and Licenses:
MTA

Comments:

This is a transfer vector (slightly lower titer, highest expression level) from Nolan lab's FELIX vector system.
The FELIX vector system consists of three plasmids - structural, envelope, and transfer vector - which are cotransfected into 293T cells to produce virus. The structural construct makes Gag-Pol and Rev (some variants also make Vif and A). Any envelope construct can be used for pseudotyping - VSV-G tends to give the highest efficiency and broadest host range. The transfer vector contains the viral LTRs and packaging signal. Any expression cassette (promoter and gene of interest) can be cloned into the vector. Inserts can be up to 8.5kB.

Please note that the AflII site is not unique, as suggested by the plasmid map and full sequence information.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 1730" in your Materials and Methods section.