Addgene - pCI-VSVG Plasmid Data

Plasmid 1733: pCI-VSVG

Gene/insert name:
VSV-G Envelope
Vector backbone:
modified pUC19
(Search Vector Database)
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
5553
5' sequencing primer:
T7 List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
Unknown
Sequence: View sequences (2)
Map:
View map
Supplemental document:
Nolan-felix (application/pdf)
Principal Investigator:
Garry Nolan
Terms and Licenses:
MTA

Comments:

This is an envelope construct and is a part of the FELIX vector system from Nolan lab. The FELIX vector system consists of three plasmids - structural, envelope, and transfer vector - which are cotransfected into 293T cells to produce virus. The structural construct makes Gag-Pol and Rev (some variants also make Vif and A). Any envelope construct can be used for pseudotyping - VSV-G tends to give the highest efficiency and broadest host range. The transfer vector contains the viral LTRs and packaging signal. Any expression cassette (promoter and gene of interest) can be cloned into the vector. Inserts can be up to 8.5kB.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 1733" in your Materials and Methods section.

Price: $65.00

Available to academic and non-profits only

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This is commonly requested with

8455: pCMV-dR8.2 dvpr