pCI-VSVG
(Plasmid #1733)

• Purpose: VSV-G expressing envelope vector; recommended for the FELIX (FIV-based) vector system from the Nolan lab
• Depositing Lab: Garry Nolan
• Publication: Nolan Lab Plasmids (unpublished)
• Sequence Information:

  • Depositor Sequences: Full (1)
  • Addgene Sequences: Partial (1)

Ordering

Item	Catalog #	Description	Quantity	Price (USD)
Plasmid	1733	Plasmid sent as bacteria in agar stab	1	$65	Add to Cart

This material is available to academics and nonprofits only.

Backbone

• Vector backbone: modified pUC19
  (Search Vector Database)
• Backbone size w/o insert (bp): 5553
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: VSV-G Envelope

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: T7

Resource Information

• Supplemental Documents:
  • Nolan-felix
• Terms and Licenses:
  • UBMTA
  • Nolan plasmid Ancillary Agreement
• Articles Citing this Plasmid:
  • 12 References

Depositor Comments

This is an envelope construct and is a part of the FELIX vector system from Nolan lab. The FELIX vector system consists of three plasmids - structural, envelope, and transfer vector - which are cotransfected into 293T cells to produce virus. The structural construct makes Gag-Pol and Rev (some variants also make Vif and A). Any envelope construct can be used for pseudotyping - VSV-G tends to give the highest efficiency and broadest host range. The transfer vector contains the viral LTRs and packaging signal. Any expression cassette (promoter and gene of interest) can be cloned into the vector. Inserts can be up to 8.5kB.