|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||1733||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector backbonemodified pUC19
- Backbone size w/o insert (bp) 5553
Vector typeMammalian Expression
Growth in Bacteria
Gene/Insert nameVSV-G Envelope
- Cloning method Restriction Enzyme
- 5′ sequencing primer T7 (Common Sequencing Primers)
This is an envelope construct and is a part of the FELIX vector system from Nolan lab. The FELIX vector system consists of three plasmids - structural, envelope, and transfer vector - which are cotransfected into 293T cells to produce virus. The structural construct makes Gag-Pol and Rev (some variants also make Vif and A). Any envelope construct can be used for pseudotyping - VSV-G tends to give the highest efficiency and broadest host range. The transfer vector contains the viral LTRs and packaging signal. Any expression cassette (promoter and gene of interest) can be cloned into the vector. Inserts can be up to 8.5kB.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCI-VSVG was a gift from Garry Nolan (Addgene plasmid # 1733 ; http://n2t.net/addgene:1733 ; RRID:Addgene_1733)