Addgene - pBMN-I-GFP Plasmid Data

Plasmid 1736: pBMN-I-GFP

Gene/insert name:
IRES-GFP
Vector backbone:
pBMN
(Search Vector Database)
Backbone manufacturer:
Nolan Lab
Vector type:
Retroviral
Backbone size w/o insert (bp):
6332
5' sequencing primer:
pBMN 5' List of Sequencing Primers
Bacterial resistance(s)
Ampicillin (50 ug/ml)
Growth strain(s)
Stbl3
Growth temperature (℃):
30
Growth instructions:
Grow in Stbl3 at 30C
High or low copy:
Low Copy
Sequence: View sequences (2)
Map:
View map
Supplemental document:
Sal1/BamH1 digest 1736 (application/pdf)
Principal Investigator:
Garry Nolan
Terms and Licenses:
MTA

Comments:

The pBMN backbone with IRES allowing for expression of GFP. Please note that the sequence information has some inaccuracies, so restriction digests may give slightly different bands than predicted.

This plasmid is extremely slow growing and may need 2 days to grow.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 1736" in your Materials and Methods section.

Price: $65.00

Available to academic and non-profits only

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