|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||1732||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Vector backbonemodified pUC119
- Backbone size w/o insert (bp) 12905
Vector typeMammalian Expression
Growth in Bacteria
Gene/Insert nameGag-Pol and Rev
SpeciesFeline Immunodeficiency Virus
- Cloning method Restriction Enzyme
- 5′ sequencing primer n/a
- 3′ sequencing primer M13 reverse primer (Common Sequencing Primers)
This is the FIV PPR based second generation packaging vector, part of the FELIX vector system from Nolan lab. The FELIX vector system consists of three plasmids - structural, envelope, and transfer vector - which are cotransfected into 293T cells to produce virus. The structural construct makes Gag-Pol and Rev (some variants also make Vif and A). Any envelope construct can be used for pseudotyping - VSV-G tends to give the highest efficiency and broadest host range. The transfer vector contains the viral LTRs and packaging signal. Any expression cassette (promoter and gene of interest) can be cloned into the vector. Inserts can be up to 8.5kB. Please note that the sequence information has some inaccuracies, so restriction digests may give slightly different bands than predicted.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCPRDEnv was a gift from Garry Nolan (Addgene plasmid # 1732 ; http://n2t.net/addgene:1732 ; RRID_Addgene_1732)