Purpose(Empty Backbone) self-inactivating lentiviral plasmid for co-expression of your gene of interest and EGFP
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||21373||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size (bp) 6900
Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
Growth instructionsGrowth in Stbl3 is preferred
Copy numberHigh Copy
- Cloning method Restriction Enzyme
- 5′ cloning site NA (unknown if destroyed)
- 3′ cloning site NA (unknown if destroyed)
- 5′ sequencing primer 5'-TGGAATTTGCCCTTTTTGAG-3'
- 3′ sequencing primer 5'-AGGAACTGCTTCCTTCACGA-3' (Common Sequencing Primers)
A portion of this plasmid was derived from a plasmid made byEGFP was from Clontech and pSICO was from Tyler Jacks.
Terms and Licenses
Articles Citing this Plasmid
HIV-EGFP is a self-inactivating lentiviral plasmid that was cloned by removing the U6-TATAlox-CMVie-EGFP-TATAlox- WPRE content of pSICO (Ventura et al., 2004), and adding the EF1-alpha promoter, a multiple cloning site (MCS), an internal ribosome entry site (IRES) and EGFP. A cDNA can be cloned into the MCS (HpaI, XbaI, SmaI, and BamHI sites) to enable bicistronic expression with EGFP. This construct was described in the publication: Cell Stem Cell. 2008 Jan 10. 2(1):90-102.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pHIV-EGFP was a gift from Bryan Welm & Zena Werb (Addgene plasmid # 21373 ; http://n2t.net/addgene:21373 ; RRID:Addgene_21373)
For your References section:Lentiviral transduction of mammary stem cells for analysis of gene function during development and cancer. Welm BE, Dijkgraaf GJ, Bledau AS, Welm AL, Werb Z. Cell Stem Cell. 2008 Jan 10. 2(1):90-102. 10.1016/j.stem.2007.10.002 PubMed 18371425