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A versatile viral system for expression and depletion of proteins in mammalian cells.
Campeau E, Ruhl VE, Rodier F, Smith CL, Rahmberg BL, Fuss JO, Campisi J, Yaswen P, Cooper PK, Kaufman PD.
PLoS One. 2009 Aug 6;4(8):e6529.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
17291pLenti CMV/TO Hygro DEST (693-2)3rd gen lentiviral Gateway destination vector, expression, CMV/TO promoter, Hygro
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17292pLenti CMV/TO Neo DEST (685-3)3rd gen lentiviral Gateway destination vector, expression, CMV/TO promoter, Neo
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17293pLenti CMV/TO Puro DEST (670-1)3rd gen lentiviral Gateway destination vector, expression, CMV/TO promoter, Puro
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17294pLenti CMV/TO Zeo DEST (644-1)3rd gen lentiviral Gateway destination vector, expression, CMV/TO promoter, Zeo
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17295pLenti X2 Hygro DEST (w17-1)3rd gen lentiviral Gateway destination vector, RNAi, 3’LTR insertion, Hygro
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17296pLenti X2 Puro DEST (w16-1)3rd gen lentiviral Gateway destination vector, RNAi, 3’LTR insertion, Puro
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17297pLenti X1 Puro DEST (694-6)3rd gen lentiviral promoter-less Gateway destination vector, Puro
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17338pENTR/pSUPER+ (575-1)Gateway entry vector for shRNA expression under H1 promoter
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17386pQCXP CMV/TO DEST (w360-1)Retroviral Gateway destination vector, Expression, CMV/TO promoter, Puro
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17387pENTR/pSM2(U6) (w51-1)Gateway entry vector for miRNA expression under U6 promoter
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17388pENTR/pSM2(CMV/TO) (w55-1)Gateway entry vector for miRNA expression under CMV/TO promoter
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17389pENTR/pSM2(CMV) (w57-1)Gateway entry vector for miRNA expression under CMV promoter
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17390pLenti X2 Neo DEST (w18-1)3rd gen lentiviral Gateway destination vector, RNAi, 3’LTR insertion, Neo
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17391pLenti X2 Blast DEST (w46-2)3rd gen lentiviral Gateway destination vector, RNAi, 3’LTR insertion, Blast
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17392pLenti CMV Neo DEST (705-1)3rd gen lentiviral Gateway destination vector, expression, CMV promoter, Neo
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17393pQCXP CMV/TO empty (w368-1)Retroviral Empty control vector, CMV/TO promoter, Puro
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17394pQCXIH CMV/TO DEST (w382-1)Retroviral Gateway destination vector, Expression, CMV/TO promoter, Hygro
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17396pENTR4-GFP-C1 (w392-1)Gateway entry vector for N-term eGFP fusion, frame C1
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17397pENTR4-GFP-C3 (w393-1)Gateway entry vector for N-term eGFP fusion, frame C3
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17398pENTR1A no ccDB (w48-1)Gateway entry 1A vector, no ccDB gene
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17399pQCXIN X2 DEST (w310-1)Retroviral Gateway destination vector, RNAi, 3’LTR insertion, Neo
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17400pQCXIB CMV/TO DEST (w320-1)Retroviral Gateway destination vector, Expression, CMV/TO promoter, Blast 
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17401pQCXIZ CMV/TO DEST (w321-2)Retroviral Gateway destination vector, Expression, CMV/TO promoter, Zeo
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17402pENTR1A-NTAP-A (w322-1)Gateway entry vector for NTAP (N-terminal affinity purification), frame A
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17403pENTR1A-NTAP-B (w323-1)Gateway entry vector for NTAP (N-terminal affinity purification), frame B
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17404pENTR1A-NTAP-C (w324-1)Gateway entry vector for NTAP (N-terminal affinity purification), frame C
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17407pENTR4-V5-2-Ubc9 (w345-2)Gateway entry vector for N-term V5 tag, frame B and Ubc9 C-term
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17413pENTR1A-FKBP12DD C-term (w350-2)Gateway entry vector for Degron, C-term
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17414pENTR4-FKBP12DD N-term A (B3-1)Gateway entry vector for Degron, N-term, frame A
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17415pENTR4-FKBP12DD N-term B (w385-4)Gateway entry vector for Degron, N-term, frame B
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17416pENTR4-FKBP12DD N-term C (w385-10)Gateway entry vector for Degron, N-term, frame C
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17423pENTR4-FLAG (w210-2)Gateway entry vector for N-term FLAG tag
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17424pENTR4 no ccDB (686-1)Gateway entry vector, no ccDB gene
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17425pENTR4-V5 (w71-3)Gateway entry vector for N-term V5 tag, frame A
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17426pENTR4-V5-2 (w234-1)Gateway entry vector for N-term V5 tag, frame B
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17427pEF-ENTR A (696-6)Gateway entry vector with EF1-a promoter, V5-His C-term, frame A
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17428pEF-ENTR B (708-1)Gateway entry vector with EF1-a promoter, V5-His C-term, frame B
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17429pEF-ENTR C (709-1)Gateway entry vector with EF1-a promoter, V5-His C-term, frame C 
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17430pEF-ENTR-LacZ (710-1)Gateway entry vector with EF1-a promoter, V5-His C-term, Lac Z
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17431pLenti CMV/TO GFP-Zeo DEST (719-1)3rd gen lentiviral Gateway destination vector, expression, CMV/TO promoter, GFP-Zeo
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17432pLenti CMV/TO DEST (775-1)3rd gen lentiviral Gateway destination vector, expression, CMV/TO promoter
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17446pLenti CMV GFP Hygro (656-4)3rd gen lentiviral eGFP expression vector, CMV promoter, Hygro
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17447pLenti CMV GFP Neo (657-2)3rd gen lentiviral eGFP expression vector, CMV promoter, Neo
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17448pLenti CMV GFP Puro (658-5)3rd gen lentiviral eGFP expression vector, CMV promoter, Puro
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17449pLenti CMV GFP Zeo (637-7)3rd gen lentiviral eGFP expression vector, CMV promoter, Zeo
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17451pLenti CMV Blast DEST (706-1)3rd gen lentiviral Gateway destination vector, expression, CMV promoter, Blast 
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17452pLenti CMV Puro DEST (w118-1)3rd gen lentiviral Gateway destination vector, expression, CMV promoter, Puro
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17453pENTR/pTER+ (430-1)Gateway entry vector for shRNA expression under H1/TO promoter
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17454pLenti CMV Hygro DEST (w117-1)3rd gen lentiviral Gateway destination vector, expression, CMV promoter, Hygro
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17455pL106P-LUC-ENTR1A(w451-1)Gateway entry vector, Degron N-term Luciferase
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17470pENTR/pTER shEGFP #1 (628-2)Gateway entry vector for EGFPshRNA#1 under H1/TO promoter
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17471pENTR/pTER shEGFP #2 (728-2)Gateway entry vector for EGFPshRNA#2 under H1/TO promoter
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17472pENTR/pTER shLUC (w177-1)Gateway entry vector for Firefly Luciferase shRNA under H1/TO promoter
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17473pENTR-LUC (w158-1)Gateway entry vector, Firefly Luciferase
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17474pQCXIP X2 DEST (w270-1)Retroviral Gateway destination vector, RNAi, 3’LTR insertion, Puro
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17475pQCXIB CMV/TO LUC (w431-1)Retroviral Luciferase Expression vector, CMV/TO promoter, Blast 
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17476pQCXP CMV/TO LUC (w432-1)Retroviral Luciferase expression vector, CMV/TO promoter, Puro 
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17477pLenti CMV Puro LUC (w168-1)3rd gen lentiviral Luciferase expression vector, CMV promoter, Puro
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17478pLenti X2 Neo empty (w36-1)3rd gen lentiviral RNAi empty control vector, 3’LTR insertion, Neo 
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17479pLenti X2 Neo/pTER shEGFP#1 (w21-2)3rd gen lentiviral eGFP shRNA #1 (H1/TO promoter), 3’LTR insertion, Neo 
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17480pLenti X2 Neo/pTER shEGFP#2 (w22-2)3rd gen lentiviral eGFP shRNA #2 (H1/TO promoter), 3’LTR insertion, Neo 
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17481pLenti CMV/TO eGFP Puro (w159-1)3rd gen lentiviral eGFP expression vector, CMV/TO promoter, Puro 
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17482pLenti CMV/TO Puro empty (w175-1)3rd gen lentiviral empty control vector, CMV/TO promoter, Puro
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17483pLenti X2 Neo/pTER shLUC (w181-1)3rd gen lentiviral Luciferase shRNA (H1/TO promoter), 3’LTR insertion, Neo 
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17484pLenti CMV/TO Hygro empty (w214-1)3rd gen lentiviral empty control vector, CMV/TO promoter, Hygro 
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17485pLenti CMV/TO Neo empty (w215-1)3rd gen lentiviral empty control vector, CMV/TO promoter, Neo
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17486pLenti CMV Blast empty (w263-1)3rd gen lentiviral empty control vector, CMV promoter, Blast 
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17487pQCXIB empty (w335-1)Retroviral Empty control vector, CMV/TO promoter, Blast 
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17488pQCXIN X2/pSM2(CMV/TO) empty (w334-1)Retroviral Empty control vector, miRNA (CMV/TO), 3’LTR insertion, Neo 
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17489pQCXIN X2/pTER shLUC (w347-1)Retroviral Luciferase shRNA (H1/TO promoter), 3’LTR insertion, Neo
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17491pQCXIP X2/pTER shLUC (w454-1)Retroviral Luciferase shRNA (H1/TO promoter), 3’LTR insertion, Puro
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17492pLenti CMV TetR Blast (716-1)3rd gen lentiviral Tetracycline repressor expression vector, CMV promoter, Blast 
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17740pQCXP CMV/TO V5-LUC (w476-1)Retroviral V5-Luciferase expression vector, CMV/TO promoter, Puro 
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17741pENTR4-GST 6P-1 (w487-1)Gateway entry vector for N-term GST fusion, frame 6P-1
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19065pLenti PGK Blast DEST (w524-1)3rd gen lentiviral Gateway destination vector, expression, PGK promoter, Blast 
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19066pLenti PGK Hygro DEST (w530-1)3rd gen lentiviral Gateway destination vector, expression, PGK promoter, Hygro
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19067pLenti PGK Neo DEST (w531-1)3rd gen lentiviral Gateway destination vector, expression, PGK promoter, Neo
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19068pLenti PGK Puro DEST (w529-2)3rd gen lentiviral Gateway destination vector, expression, PGK promoter, Puro
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19069pLenti PGK GFP Blast (w510-5)3rd gen lentiviral eGFP expression vector, PGK promoter, Blast
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19070pLenti PGK GFP Puro (w509-5)3rd gen lentiviral eGFP expression vector, PGK promoter, Puro 
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19135pENTR4-V5-LUC (w468-1)Gateway entry vector, V5-Luciferase
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19166pLenti PGK Blast V5-LUC (w528-1)3rd gen lentiviral V5-Luciferase expression vector, PGK promoter, Blast
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19167pQCXIN X2/pTER shEGFP#1 (w491-1)Retroviral eGFP shRNA #2 (H1/TO promoter), 3’LTR insertion, Neo
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19170pENTR/pSM2(CMV) GFP (w513-1)Gateway entry vector for miRNA expression under CMV promoter and GFP reporter
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19360pLenti PGK V5-LUC Puro (w543-1)3rd gen lentiviral V5-Luciferase expression vector, PGK promoter, Puro
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19364pENTR1A-GFP-N2 (FR1)Gateway entry vector for C-term eGFP fusion, frame N2
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19732pLenti CMV GFP DEST (736-1)3rd gen lentiviral Gateway destination vector, expression/RNAi, eGFP co-expression
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19785pLenti CMV/TO V5-LUC Puro (w549-1)3rd gen lentiviral V5-Luciferase expression vector, CMV/TO promoter, Puro
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20025pENTR4-HA-myc-nuc (w557-1)Gateway entry vector for nuclear targeting of domains (N-term HA , C-term NLS, myc tags)
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20026pENTR4-myc-nuc (w556-1)Gateway entry vector for nuclear targeting of domains (C-term NLS, myc tags)
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Antibodies from Article