Addgene - pLenti X2 Neo/pTER shEGFP#2 (w22-2) Plasmid Data

Plasmid 17480: pLenti X2 Neo/pTER shEGFP#2 (w22-2)

Gene/insert name:
Enhanced green fluorescent protein shRNA #2
Alt name:
shGFP #2
Insert size:
66
Vector backbone:
p156RRL-sinPPT-CMV-GFP-PRE/Nhe I
(Search Vector Database)
Vector type:
Lentiviral, RNAi
Backbone size w/o insert (bp):
7919
Cloning site 5':
Bgl II
Site destroyed during cloning:
Yes
Cloning site 3':
Hind III
Site destroyed during cloning:
No
5' sequencing primer:
n/a List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
Stbl3
Growth temperature (℃):
37
Growth instructions:
Stbl3, 37OC
High or low copy:
High Copy
Selectable markers:
Neomycin
Sequence: View sequences (3)
Map:
View map
Principal Investigator:
Eric Campeau
Terms and Licenses:
MTA

Comments:

shRNa sequence - CCGCTGGAGTACAACTACAACTTCAAGAGAGTTGTAGTTGTACTCCAGCTTTTT

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: A versatile viral system for expression and depletion of proteins in mammalian cells. Campeau et al (PLoS One. 2009 Aug 6;4(8):e6529. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 17480" in your Materials and Methods section.