pLenti CMV Neo DEST (705-1)
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||17392||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
|Cloning Grade DNA||17392-DNA.cg||2 µg of cloning grade DNA in Tris buffer||1||$95|
This material is available to academics and nonprofits only.
Vector backbonep156RRL-sinPPT-CMV-GFP-PRE/Nhe I
- Backbone size (bp) 9796
Vector typeMammalian Expression, Lentiviral ; Destination vector
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Bacterial Resistance(s)Chloramphenicol and Ampicillin
Growth Strain(s)ccdB Survival
Growth instructionsAlso resistant to Chloramphinecol, DB3.1 or ccDB survival, 37oC
Copy numberHigh Copy
- Cloning method Restriction Enzyme
- 5′ sequencing primer CMVforw (Common Sequencing Primers)
Please note that Addgene's quality control sequence shows several gaps with depositor's reference sequence. These gaps are in vector region only and should not affect function.
Information for Cloning Grade DNA (Catalog # 17392-DNA.cg) ( Back to top )
Cloning grade DNA is suitable for use in PCR, cloning reactions, or transformation into E. coli. The purity and amount is not suitable for direct transfections.
- Amount 2 µg
- Guaranteed Concentration 100 ng/µl +/- 5 ng/µl
- Pricing $95 USD
- Storage DNA can be stored at 4℃ (short term) or -20℃ (long term).
Terms and Licenses
- Not Available to Industry
Addgene has verified this plasmid using Next Generation Sequencing. Results are available here
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pLenti CMV Neo DEST (705-1) was a gift from Eric Campeau & Paul Kaufman (Addgene plasmid # 17392 ; http://n2t.net/addgene:17392 ; RRID:Addgene_17392)
For your References section:A versatile viral system for expression and depletion of proteins in mammalian cells. Campeau E, Ruhl VE, Rodier F, Smith CL, Rahmberg BL, Fuss JO, Campisi J, Yaswen P, Cooper PK, Kaufman PD.. PLoS One. 2009 Aug 6;4(8):e6529. 10.1371/journal.pone.0006529 PubMed 19657394