Addgene - pLenti CMV Blast DEST (706-1) Plasmid Data

Plasmid 17451: pLenti CMV Blast DEST (706-1)

Gene/insert name:
None
Vector backbone:
p156RRL-sinPPT-CMV-GFP-PRE/Nhe I
(Search Vector Database)
Vector type:
Mammalian Expression, Lentiviral ; Destination vector
Backbone size (bp):
9331
5' sequencing primer:
CMVforw List of Sequencing Primers
Bacterial resistance(s)
Ampicillin and Chloramphenicol
Growth strain(s)
DB3.1
Growth temperature (℃):
37
Growth instructions:
Use 50ug/mL Amp
High or low copy:
High Copy
Selectable markers:
Blasticidin
Sequence: View sequences (3)
Map:
View map
Principal Investigator:
Eric Campeau
Terms and Licenses:
MTA

Comments:

There are 2 gaps between depositor's reference sequence and Addgene's quality control sequence. The gaps are in vector regions only and do not affect any of the features.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: A versatile viral system for expression and depletion of proteins in mammalian cells. Campeau et al (PLoS One. 2009 Aug 6;4(8):e6529. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 17451" in your Materials and Methods section.