1
Plasmid 17454: pLenti CMV Hygro DEST (w117-1)
  • 3rd gen lentiviral Gateway destination vector, expression, CMV promoter, Hygro

  • None

  • p156RRL-sinPPT-CMV-GFP-PRE/Nhe I
    (Search Vector Database)

  • Mammalian Expression, Lentiviral ; Destination vector

  • 10319

  • CMVforw List of Sequencing Primers

  • Ampicillin and Chloramphenicol

  • DB3.1

  • 37

  • DB3.1 or ccDB survival, 37oC

  • High Copy

  • Hygromycin

  • View sequences (2)
  • View map

  • Eric Campeau

  • MTA

Comments: 

Please note that there are several mismatches between depositor's sequence and Addgene's quality control sequence. These mismatches are in the backbone regions only and do not affect any of the features.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: A versatile viral system for expression and depletion of proteins in mammalian cells. Campeau et al (PLoS One. 2009 Aug 6;4(8):e6529. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 17454" in your Materials and Methods section.