pLenti PGK Hygro DEST (w530-1)
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||19066||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector backbonep156RRL-sinPPT-CMV-GFP-PRE/Nhe I
- Backbone size (bp) 10301
Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
Bacterial Resistance(s)Chloramphenicol and Ampicillin
Growth Strain(s)ccdB Survival
Growth instructionsAlso resistant to Chloramphinecol. DB3.1 or ccDB survival, 37oC
Copy numberHigh Copy
- Cloning method Restriction Enzyme
- 5′ sequencing primer PGKforw (Common Sequencing Primers)
Terms and Licenses
Articles Citing this Plasmid
There are several mismatches between Addgene's quality control sequence and depositor's reference sequence. The mismatches are in non-coding regions and do not affect the features.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pLenti PGK Hygro DEST (w530-1) was a gift from Eric Campeau & Paul Kaufman (Addgene plasmid # 19066 ; http://n2t.net/addgene:19066 ; RRID:Addgene_19066)
For your References section:A versatile viral system for expression and depletion of proteins in mammalian cells. Campeau E, Ruhl VE, Rodier F, Smith CL, Rahmberg BL, Fuss JO, Campisi J, Yaswen P, Cooper PK, Kaufman PD.. PLoS One. 2009 Aug 6;4(8):e6529. 10.1371/journal.pone.0006529 PubMed 19657394