Addgene - pLenti PGK Hygro DEST (w530-1) Plasmid Data

Plasmid 19066: pLenti PGK Hygro DEST (w530-1)

Gene/insert name:
None
Vector backbone:
p156RRL-sinPPT-CMV-GFP-PRE/Nhe I
(Search Vector Database)
Vector type:
Mammalian Expression, Lentiviral
Backbone size (bp):
10301
5' sequencing primer:
PGKforw List of Sequencing Primers
Bacterial resistance(s)
Ampicillin (50 ug/ml)
Growth strain(s)
DB3.1
Growth temperature (℃):
37
Growth instructions:
Also resistant to Chloramphinecol. DB3.1 or ccDB survival, 37oC
High or low copy:
High Copy
Selectable markers:
Hygromycin
Sequence: View sequences (2)
Map:
View map
Principal Investigator:
Eric Campeau
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: A versatile viral system for expression and depletion of proteins in mammalian cells. Campeau et al (PLoS One. 2009 Aug 6;4(8):e6529. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 19066" in your Materials and Methods section.