pLenti CMV GFP Puro (658-5)
Vector backbonep156RRL-sinPPT-CMV-GFP-PRE/Nhe I
- Backbone size w/o insert (bp) 7889
Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
Growth instructionsStbl3, 37oC
Copy numberHigh Copy
Alt nameenhanced GFP
Insert Size (bp)719
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site Sal I (not destroyed)
- 5′ sequencing primer CMVforw (Common Sequencing Primers)
Terms and Licenses
The 3'LTR of this plasmid has a TT (or AA on the sense strand) in the sequence. This sequence is consistent with other available LTR sequences and should not affect plasmid function.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pLenti CMV GFP Puro (658-5) was a gift from Eric Campeau (Addgene plasmid # 17448)
For your References section:A versatile viral system for expression and depletion of proteins in mammalian cells. Campeau E, Ruhl VE, Rodier F, Smith CL, Rahmberg BL, Fuss JO, Campisi J, Yaswen P, Cooper PK, Kaufman PD.. PLoS One. 2009 Aug 6;4(8):e6529. 10.1371/journal.pone.0006529 PubMed 19657394
Map generated by Addgene from full sequence supplied by depositor.
Map uploaded by the depositor.