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Plasmid 17448: pLenti CMV GFP Puro (658-5)
  • 3rd gen lentiviral eGFP expression vector, CMV promoter, Puro

  • GFP

  • enhanced GFP

  • 719

  • p156RRL-sinPPT-CMV-GFP-PRE/Nhe I
    (Search Vector Database)

  • Mammalian Expression, Lentiviral

  • 7889

  • BamHI

  • No

  • Sal I

  • No

  • CMVforw List of Sequencing Primers

  • Ampicillin

  • Stbl3

  • 37

  • Stbl3, 37oC

  • High Copy

  • Puromycin

  • View sequences (8)
  • View map

  • Eric Campeau

  • UBMTA
    Ancillary Agreement for Plasmids Containing FP Materials

Comments: 

The 3'LTR of this plasmid has a TT (or AA on the sense strand) in the sequence. This sequence is consistent with other available LTR sequences and should not affect plasmid function.

Addgene has sequenced a portion of this plasmid for verification. Full plasmid sequence is available only if provided by the depositing laboratory.

Article: A versatile viral system for expression and depletion of proteins in mammalian cells. Campeau et al (PLoS One. 2009 Aug 6;4(8):e6529. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 17448" in your Materials and Methods section.

Price: US $65

Available to academic and non-profits only